Bee venom (Apis mellifera L.) rescues zinc oxide nanoparticles induced neurobehavioral and neurotoxic impact via controlling neurofilament and GAP-43 in rat brain

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(38), P. 88685 - 88703

Published: July 13, 2023

This study investigated the possible beneficial role of bee venom (BV, Apis mellifera L.) against zinc oxide nanoparticles (ZNPs)-induced neurobehavioral and neurotoxic impacts in rats. Fifty male Sprague Dawley rats were alienated into five groups. Three groups intraperitoneally injected distilled water (C 28D group), ZNPs (100 mg/kg b.wt) (ZNPs or mg/kg.wt) BV (1 mg/ kg.bwt) + group) for 28 days. One group was with 1 mL 56 days 56D group). The last days, then another at same earlier doses duration (ZNPs/BV Depression, anxiety, locomotor activity, spatial learning, memory evaluated using forced swimming test, elevated plus maze, open field Morris maze respectively. brain contents dopamine, serotonin, total antioxidant capacity (TAC), malondialdehyde (MDA), Zn estimated. histopathological changes immunoexpressions neurofilament GAP-43 protein tissues followed. results displayed that significantly decreased ZNPs-induced depression, impairment, learning disorders. Moreover, increment serotonin dopamine levels content suppressed by BV. Besides, restored depleted TAC but minimized augmented MDA associated exposure. Likewise, neurodegenerative induced abolished Also, increased immunoexpression due to exposure alleviated Of note, achieved better than ZNPs/BV group. Conclusively, these demonstrated could be employed as a biologically effective therapy mitigate effects ZNPs, particularly when used during

Language: Английский

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Phyto-mediated synthesis of zinc oxide nanoparticles from Clerodendrum infortunatum L. leaf extract and evaluation of antibacterial potential

South African Journal of Botany, Journal Year: 2023, Volume and Issue: 164, P. 146 - 151

Published: Dec. 2, 2023

Language: Английский

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Ammodaucus leucotrichus Coss. & Dur. Attenuates acrylamide-induced hepatic injury through enhancing autophagy and inhibiting endoplasmic reticulum stress and apoptotic signaling

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 115, P. 106090 - 106090

Published: March 7, 2024

In our study, we investigated the potential of Ammodaucus leucotrichus Coss. & Dur. (Al) seed extract and essential oil (Al oil) to mitigate acrylamide-induced liver injury in rats. We administered (100 200 mg/kg), (0.25 mL/kg) or reference drug (silymarin, 100 mg/kg) for four days. On fourth day, was induced by acrylamide (25 mg/kg, PO), continuing treatment seven more assessed enzymes, oxidative endoplasmic reticulum (ER) stress markers, apoptotic autophagy conducted histopathological examinations. The ameliorated functional structural alterations caused acrylamide, reduced ER stress, inhibited apoptosis, enhanced autophagy. high-dose showed effects like silymarin, potentially attributed its phenolic contents. These activities indicated hepatoprotective A. against xenobiotics-induced damage, supporting their use as nutraceuticals stress-related disorders.

Language: Английский

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Thymol’s modulation of cellular macromolecules, oxidative stress, DNA damage, and NF-kB/Caspase-3 signaling in the liver of imidacloprid-exposed rats

Environmental Toxicology and Pharmacology, Journal Year: 2024, Volume and Issue: 109, P. 104492 - 104492

Published: June 3, 2024

Language: Английский

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Acrylamide-targeting renal miR−21a−5p/Fibrotic and miR122-5p/ inflammatory signaling pathways and the role of a green approach for nano-zinc detected via in silico and in vivo approaches

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 17, 2024

Introduction: Any disruption in renal function can have cascading effects on overall health. Understanding how a heat-born toxicant like acrylamide (ACR) affects kidney tissue is vital for realizing its broader implications systemic Methods: This study investigated the ACR-induced damage mechanisms, particularly focusing regulating role of miR-21a-5p/fibrotic and miR-122-5p/inflammatory signaling pathways via targeting Timp-3 TP53 proteins an In silico preliminary study. Besides, assessment, oxidative status, protein profile, expression biomarkers (Timp-1, Keap-1, Kim-1, P53, TNF-α, Bax, Caspase3) were assessed 60-day experiment. The examination was additionally extended to explore potential protective green-synthesized zinc oxide nanoparticles (ZNO-MONPs). A four-group experiment including control, ZNO-MONPs (10 mg/kg b.wt.), ACR (20 + established encompassing biochemical, histological, molecular levels. further protein-binding ability ZNO MONPs inactivate caspase-3, TNFRS-1A. Results: significantly reduced as evidenced by increased serum creatinine, uric acid, albumin, stress markers. stress, apoptosis, inflammationare revealed biochemical tests, gene expression, presence apoptotic nuclei microscopically. Also, docking binding affinity between ACR-BCL-2 glutathione-synthetase, elucidating mechanisms through which induces damage. Notably, against Zn levels tissues ACR-exposed rats restored those treated with ZNO-MONPs. conclusion, this establishes efficacy mitigating disturbances functions, inflammation, apoptosis. findings shed light renoprotective activity nanomaterials, offering insights into novel therapeutic approaches countering

Language: Английский

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Restorative effects of gallic acid against sub-chronic hepatic toxicity of co-exposure to zinc oxide nanoparticles and arsenic trioxide in male rats

Heliyon, Journal Year: 2023, Volume and Issue: 9(6), P. e17326 - e17326

Published: June 1, 2023

This study aimed to assess the effect of zinc oxide nanoparticles (ZNPs) and/or arsenic trioxide (ATO) exposure on liver adult male Sprague Dawley rats. Moreover, probable ameliorative impact gallic acid (GA) against ZNPs and ATO-induced hepatotoxicity possible underlying mechanisms were evaluated.Sixty rats distributed into six groups. The 1st 2nd groups orally given distilled water (1 ml/kg) 20 mg GA/kg b. wt, respectively. 3rd 4th 100 ZNPs/kg wt 8 ATO/kg 5th group was co-administered ATO at doses mentioned above. last one ZNPs, ATO, GA earlier described doses. All tested compounds once a day for 60 successive days. Then, serum levels alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate (AST), total, direct, indirect bilirubin, triglycerides, total cholesterol, HDL, VLDL, LDL estimated. hepatic content malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) evaluated. Bcl-2 Bax's reactive proteins immunohistochemically detected, Zn As residual patterns in tissues assessed.ZNPs, ZNPs+ATO-exposed showed significantly (P < 0.001) elevated AST (219%, 233%, 333%), ALT (300%, 400%, 475%), ALP (169%, 205%, 294%), bilirubin (42%, 68%, 109%) compared control ones. On other hand, declined SOD (58%, 49%, 43%) GPx (70%, 63%, 56%) but increased MDA (133%, 150%, 224%) recorded ZNPs+ATO exposed rats, respectively, relative significant decrease (28%, 33%, 23%) elevation Bax (217%, 267%, 236%) immunoreactivities These findings consistent with microscopic alterations architecture accumulation As. Furthermore, notable hyperlipidemic condition following exposure. contrary, notably reduced enzymes Additionally, markedly improved ZNPs+ATO-afforded tissue damage apoptotic events.Overall, oral dosing mitigated negative effects by improving antioxidant defense system controlling changes.

Language: Английский

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Thymoquinone Abrogates Acrylamide-Induced Cerebellar Toxicity via Modulation of Nuclear Factor Erythroid 2-Related Factor 2/Nuclear Factor Kappa B Signaling, Oxidative Neuroinflammation, and Neuroapoptosis in Rats

Journal of Medicinal Food, Journal Year: 2024, Volume and Issue: 27(11), P. 1062 - 1069

Published: Sept. 25, 2024

Acrylamide (ACR) is an obligate human neurotoxicant ubiquitously produced and found in foods processed at high temperature. There increasing public health concern regarding its probable carcinogenic potential. Its prevailing toxicity mechanism oxidative inflammation apoptosis. Herein, we explored whether thymoquinone (TQ), a bioactive quinone

Language: Английский

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Biofabricated zinc oxide nanoparticles mitigate acrylamide-induced immune toxicity and modulate immune-related genes and microRNA in rats

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Language: Английский

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Effects of dietary acrylamide on kidney and liver health: Molecular mechanisms and pharmacological implications

Toxicology Reports, Journal Year: 2024, Volume and Issue: 14, P. 101859 - 101859

Published: Dec. 10, 2024

Acrylamide (AA) has raised concerns throughout the world in recent years because of its potential negative effects on human health. Numerous researches humans and animals have connected a high dietary exposure to AA possible risk cancer. Additionally, higher consumption acrylamide also been associated with dysfunctioning various organ systems from nervous system reproductive system. is primarily metabolised into glycidamide inside body which gets accumulated different tissues including kidney liver, chronic this can lead nephrotoxicity hepatotoxicity through molecular mechanisms. This review summarizes sources, formation metabolism along mechanisms such as oxidative stress, inflammation, DNA damage, autophagy, mitochondrial dysfunction morphological changes nephron hepatocytes exerts deleterious effect liver causing hepatotoxicity. animal cellular studies that demonstrate AA-induced Lastly, article emphasizes underlying protective pharmacological interventions against induced nephrotoxicity.

Language: Английский

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