Although
bacteriophage-based
biosensors
are
promising
tools
for
rapid,
convenient,
and
sensitive
detection
of
Staphylococcus
aureus
in
food
products,
the
effect
using
temperate
phages
as
biorecognition
elements
to
detect
viable
S.
isolates
remains
unclear.
In
this
study,
three
were
isolated,
their
biological
features
(one-step
growth,
host
range,
pH
stability,
temperature
adsorption
rate)
evaluated
element.
The
selected
phage
SapYZUs8
was
immobilized
on
Cu-MOF
nanozyme
via
electrostatic
interactions
prepare
SapYZUs8@Cu-MOF;
its
performance
real
(skim
milk
pork)
evaluated.
Phage
exhibited
a
higher
titre
(6.2×108
PFU/mL),
broader
stronger
stability
(3–12),
outstanding
absorption
rate
(92%,
8
min)
suitable
contained
unique
gene
content
associated
with
DNA
replication
tail
protein.
Therefore,
fixed
SapYZUs8@Cu-MOF,
which
good
sensitivity
specificity
rapid
colourimetric
determination
aureus.
method
took
<0.5
h,
limit
1.09×102
CFU
mL-1.
addition,
SapYZUs8@Cu-MOF
successfully
employed
samples
while
ignoring
different
additives,
NaCl
concentrations,
values.
With
these
benefits,
it
provides
visual
inspection
ACS Applied Bio Materials,
Год журнала:
2024,
Номер
7(3), С. 1643 - 1655
Опубликована: Фев. 17, 2024
Pathogens
and
pests
pose
significant
threats
to
global
crop
productivity
plant
immunity,
necessitating
urgent
measures
from
researchers
prevent
pathogen
contamination
pest
damage
crops.
A
natural
plant-based
antibacterial
agent,
eugenol
(EUG),
has
demonstrated
excellent
antimicrobial
insect
repellent
capabilities,
but
the
characteristics
of
volatilization
poor
dissolution
limit
practical
application.
The
nanoization
pesticide
formulations
holds
promise
in
development
highly
effective
pesticides
for
insecticidal
purposes.
Herein,
a
eugenol-loaded
nano
delivery
system
(EUG@CMC-PGMA-CS)
was
synthesized
using
glycidyl
methacrylate
(GMA)
as
functional
monomer
connect
carrier
core
structure
carboxymethyl
cellulose
(CMC)
with
shell
chitosan
(CS),
EUG
encapsulated
within
carrier.
EUG@CMC-PGMA-CS
leaf
affinity,
minimum
contact
angles
(CAs)
37.83
70.52°
on
hydrophilic
hydrophobic
vegetable
surfaces,
respectively.
Moreover,
maximum
liquid
holding
capacity
(LHC)
both
surfaces
demonstrates
noteworthy
55.24%
enhancement
compared
LHC
pure
EUG.
vitro
release
curve
exhibited
an
initial
burst
followed
by
stable
sustained
release.
It
is
satisfaction
that
exceptional
properties
against
S.
aureus
satisfactory
efficacy
Spodoptera
litura.
this
potential
enhanced
repellents
agriculture,
paving
way
application
volatile
bioactive
substances.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(41), С. 15146 - 15152
Опубликована: Сен. 21, 2023
The
landscape
of
diagnostic
assessments
has
experienced
a
paradigm
shift
driven
by
the
advent
isothermal
amplification
techniques
on
point-of-care
testing
(POCT).
development
compact,
portable
devices
further
emphasizes
their
transformative
influence
approaches.
However,
in
prioritizing
portability,
these
may
exhibit
limitations
functionality,
rendering
them
less
effective
addressing
urgent
public
health
emergencies
during
sudden
pathogen
outbreaks.
In
this
paper,
an
efficient
fluorescence
device
been
fabricated
for
rapid
detection
pathogens
crises.
features
multichannel
capability
simultaneous
various
targets,
integrates
with
Internet
Medical
Things
(IoMT)
remote
control
and
data
uploading,
includes
deep
learning-based
batch
processing
system
(9.4
ms)
accurate
discrimination
type
excellent
accuracy.
successfully
employed
to
simultaneously
detect
Staphylococcus
aureus
(SA)
methicillin-resistant
(MRSA)
limits
(LODs)
18
CFU/mL
20
within
35
min
multiplex
RPA
assay
CRISPR/Cas12a-mediated
nucleic
acid
assay.
