Ecotoxicology and Environmental Safety,
Год журнала:
2023,
Номер
258, С. 114983 - 114983
Опубликована: Май 4, 2023
Water
pollution
caused
by
widely
used
agricultural
pesticide
chlorpyrifos
(CPF)
has
aroused
extensive
public
concern.
While
previous
studies
have
reported
on
toxic
effect
of
CPF
aquatic
animal,
little
is
known
about
its
common
carp
(Cyprinus
carpio
L.)
livers.
In
this
experiment,
we
exposed
to
(11.6
μg/L)
for
15,
30,
and
45
days
establish
a
poisoning
model.
Histological
observation,
biochemical
assay,
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR),
Western
blot,
integrated
biomarker
response
(IBR)
were
applied
assess
the
hepatotoxicity
in
carp.
Our
results
displayed
that
exposure
damaged
histostructural
integrity
induced
liver
injury
Furthermore,
found
CPF-induced
may
be
associated
with
mitochondrial
dysfunction
autophagy,
as
evidenced
swollen
mitochondria,
broken
ridges,
increased
number
autophagosomes.
Moreover,
decreased
activities
ATPase
(Na+/K+-ATPase,
Ca2+-ATPase,
Mg2+-ATPase,
Ca2+Mg2+-ATPase),
altered
glucose
metabolism-related
genes
(GCK,
PCK2,
PHKB,
GYS2,
PGM1,
DLAT),
activated
energy-sensing
AMPK,
indicating
energy
metabolism
disorder.
The
activation
AMPK
further
mitophagy
via
AMPK/Drp1
pathway,
autophagy
AMPK/mTOR
pathway.
Additionally,
oxidative
stress
(abnormal
levels
SOD,
GSH,
MDA,
H2O2)
livers,
which
contributed
induction
autophagy.
Subsequently,
confirmed
time-dependent
IBR
assessment.
findings
presented
new
insight
into
molecular
mechanism
induced-hepatotoxicity
carp,
provided
theoretical
basis
evaluating
toxicity
organisms.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 24, 2023
Aquatic
organisms
such
as
fish
can
accumulate
high
concentrations
of
arsenic
(As),
which
has
toxic
effects
on
fish.
However,
whether
the
intestinal
flora
are
involved
in
As
damage
to
tissues
and
underlying
process
unclear.
Common
carp
(Cyprinus
carpio)
were
exposed
(2.83
mg/L)
water
for
30
days,
blood,
muscle,
intestine,
intestine
samples
collected.
Intestinal
pathological
sections
observed,
lipopolysaccharide
(LPS)
levels
serum
accumulation
tight
junction-related
factors
measured.
The
gut
microbiota
was
analysed
by
16S
rRNA
sequencing.
results
showed
that
treatment
decreased
abundance
microbiota,
increased
number
harmful
bacteria,
beneficial
bacteria
intestine.
In
our
experiment,
top
with
highest
relative
identified.
Among
resulted
a
significant
(P
<
0.05)
increase
(such
Fusobacteriota,
Bacteroidota
(LPS-producing
bacteria),
Verrucomicrobiota,
Bacteroides,
Aeromonas,
Stenotrophomonas)
decrease
Actinobacteriota,
Planctomycetota,
Firmicutes,
Reyranella,
Akkermansia,
Pseudorhodobacter),
further
demonstrated
affects
flora.
addition,
exposure
LPS
level
bacteria)
exhibits
six
at
phylum
level,
indicates
produced
serum.
Additionally,
protein
gene
junction
markers
ZO-1
occludin
reduced
treatment,
indicated
impaired
structural
integrity
conclusion,
obtained
study
indicate
flora,
LPS,
junctions
participate
impairment
common
resulting
from
exposure.
Comparative Immunology Reports,
Год журнала:
2024,
Номер
6, С. 200127 - 200127
Опубликована: Янв. 12, 2024
Selenium
(Se)
is
an
essential
trace
microelement
required
for
the
overall
health
of
humans
and
animals.
The
importance
Se
mainly
related
to
its
participation
in
structure
selenoproteins
with
diverse
biological
functions,
including
antioxidant
defense,
immunity,
thyroid
hormone
metabolism.
functionality
depends
on
chemical
form
(inorganic
organic
Se).
Due
low
toxicity
higher
efficacy,
nanoparticles
(nano-Se)
have
been
recently
applied
aquafeeds
enhance
fish
performance.
New
technological
advances
offered
different
Omics
approaches,
such
as
transcriptomics,
proteomics,
metabolomics,
realize
molecular
mechanisms
underlying
processes.
In
recent
years,
approaches
employed
study
nano-Se
effects
fish.
present
article
summarizes
impacts
supplementation
performance,
then
reviews
qRT-PCR
assay
Omics-based
used
dietary
Toxics,
Год журнала:
2023,
Номер
11(9), С. 734 - 734
Опубликована: Авг. 25, 2023
Lead
(Pb),
a
hazardous
heavy
metal,
can
damage
the
health
of
organisms.
However,
it
is
not
clear
whether
Pb
chicken
cerebellums
and
thalami.
Selenium
(Se),
an
essential
nutrient
for
organisms,
has
palliative
effect
on
poisoning
in
chickens.
In
our
experiment,
model
chickens
treated
with
Se
alone
combination
was
established
to
investigate
molecular
mechanism
alleviating
Pb-caused
both
Our
morphological
results
indicated
that
caused
apoptotic
lesions,
such
as
mitochondrial
nuclear
damage.
Further,
anti-apoptotic
gene
Bcl-2
decreased;
contrary,
four
pro-apoptotic
genes
(p53,
Bax,
Cyt
c,
Caspase-3)
increased
under
treatment,
meaning
apoptosis
via
p53-Cyt
c-Caspase-3
pathway.
Furthermore,
we
further
demonstrated
elevated
HSPs
(HSP27,
HSP40,
HSP70,
HSP90),
well
HSP70
took
part
apoptosis.
addition,
found
exposure
led
oxidative
stress
up-regulating
oxidant
H2O2
down-regulating
antioxidants
(CAT,
SOD,
GST,
GPx).
Moreover,
decreased
three
Se-containing
factors
(Txnrd1,
Txnrd2,
Txnrd3),
confirming
stress.
Interestingly,
supplementation
reversed
above
changes
by
alleviated
Pb-induced
A
time
dependency
Bcl-2,
c
cerebellums,
CAT,
GPx,
p53
thalami
Pb-exposed
HSP27
were
more
sensitive
than
those
respectively,
exposure.
severe
cerebellums.
conclusion,
after
Txnrds
mediated
stress,
up-regulated
HSPs,
finally,
triggered
antagonized
pathway
selenoproteins
This
study
provides
new
information
environmental
pollutant
detoxification
abiotic
