Molecules,
Год журнала:
2025,
Номер
30(4), С. 830 - 830
Опубликована: Фев. 11, 2025
This
study
optimized
the
extraction
process
of
crude
polysaccharides
from
Panax
notoginseng
leaves
(PNLP)
using
ultrasonic-assisted
dual-enzyme
method
through
a
single-factor
combined
with
response
surface
experiment.
The
were
subsequently
purified
and
isolated
DEAE-Cellulose
52,
followed
by
structural
analysis,
evaluation
antioxidant
activity,
examination
digestive
enzyme
inhibition.
hypoglycemic
effects
components
further
clarified.
results
indicated
that
polysaccharide
had
an
yield
17.13
±
0.29%.
fraction
PNLP-3
(eluted
0.3
M
NaCl)
was
obtained
52
chromatography,
exhibiting
total
sugar
content
81.2%
molecular
weight
16.57
kDa.
PNLP
is
primarily
composed
arabinose,
galactose,
galacturonic
acid,
molar
percentages
20.24%,
33.54%,
24.27%,
respectively.
mainly
arabinose
29.97%
49.35%,
In
this
IC50
values
for
α-glucosidase
α-amylase
inhibition
1.045
mg/mL
9.53
mg/mL,
Molecular
docking
confirmed
exhibits
better
inhibitory
activity
against
α-glucosidase.
Furthermore,
alleviated
hyperglycemia
in
insulin-resistant
HepG2
cells
enhancing
glucose
consumption
glycogen
synthesis.
exhibited
positive
correlation
its
concentration,
potentially
contributing
to
reducing
oxidative
stress.
These
findings
underscore
therapeutic
potential
leaf
managing
type
2
diabetes
offer
new
perspectives
on
use
natural
regulating
blood
glucose.
Ultrasonics Sonochemistry,
Год журнала:
2024,
Номер
107, С. 106918 - 106918
Опубликована: Май 18, 2024
In
this
research,
the
extraction
process
of
polysaccharides
from
Pithecellobium
clypearia
Benth
(PCBPs)
was
optimized
using
dual-frequency
ultrasound-assisted
(DUAE).
The
biological
activities
PCBPs
were
investigated
by
in
vitro
antioxidant,
hypoglycemic,
and
anti-hyperlipidemic
assay.
High-performance
anion-exchange
chromatography,
high-performance
gel
permeation
SEM,
UV–Vis
spectroscopy,
FT-IR
spectra
used
to
analyze
monosaccharide
composition,
molecular
weight,
microscopic
morphology,
characteristic
structure
PCBPs.
results
showed
that
maximum
rate
9.90
±
0.16
%
when
ultrasonic
time
8
min,
liquid-to-material
ratio
32
mL/g,
power
510
W.
also
possessed
excellent
activities.
addition,
average
weight
15.07
kDa.
consisted
rhamnose,
arabinose,
galactose,
glucose,
xylose,
mannose,
glucuronic
acid,
with
molar
ratios
11.07
%,
18.54
48.17
10.44
4.62
4.96
2.20
respectively.
Moreover,
SEM
mainly
a
fine
spherical
mesh
structure.
above
studies
provided
valuable
theoretical
basis
for
subsequent
in-depth
study
Precision medicine and engineering.,
Год журнала:
2024,
Номер
1(1), С. 100001 - 100001
Опубликована: Июль 28, 2024
Stimulus-responsive
hydrogels
can
undergo
controllable
shape
deformation
and
exhibit
sol-gel
transition
behavior
under
stimulus
signals
such
as
pH,
reactive
oxygen
species
(ROS),
heat,
light.
Polysaccharides
have
become
ideal
candidates
for
constructing
stimulus-responsive
due
to
their
biocompatibility
biodegradability.
The
diversity
of
molecular
weights
functional
groups
polysaccharides
allows
them
self-assemble
or
cooperate
with
other
materials
obtain
through
physical
chemical
crosslinking.
Bioactive
ingredients
nanomaterials
be
conveniently
encapsulated
in
the
hydrogel
matrix
meet
various
requirements.
polysaccharide-based
are
widely
used
biomedical
field,
especially
fields
drug
delivery,
tissue
engineering,
biosensors,
imaging.
In
this
review,
design
strategies
recent
research
advances
stimuli-responsive
summarized.
Future
challenges
also
presented,
review
will
guide
study
polysaccharide
hydrogels.
