Life Sciences, Год журнала: 2024, Номер 358, С. 123198 - 123198
Опубликована: Окт. 31, 2024
Язык: Английский
Journal of Neuroinflammation, Год журнала: 2023, Номер 20(1)
Опубликована: Окт. 10, 2023
Abstract Neuroinflammation is a complex biological process that plays significant role in various brain disorders. Microglia and astrocytes are the key cell types involved inflammatory responses central nervous system. results increased levels of secreted factors, such as cytokines, chemokines, reactive oxygen species. To model neuroinflammation vitro, human induced pluripotent stem (iPSC)-based models have been utilized, including monocultures, transfer conditioned media between types, co-culturing multiple neural organoids, xenotransplantation cells into mouse brain. induce neuroinflammatory several stimuli established can either microglia, astrocytes, or both. Here, we describe critically evaluate different iPSC be used to study highlight how has measured these cultures.
Язык: Английский
Процитировано
24
Microchimica Acta, Год журнала: 2024, Номер 191(1)
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
12
Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18
Опубликована: Июль 9, 2024
In 2013, M. Lancaster described the first protocol to obtain human brain organoids. These organoids, usually generated from human-induced pluripotent stem cells, can mimic three-dimensional structure of brain. While they recapitulate salient developmental stages brain, their use investigate onset and mechanisms neurodegenerative diseases still faces crucial limitations. this review, we aim highlight these limitations, which hinder organoids becoming reliable models study such as Alzheimer’s disease (AD), Parkinson’s (PD), amyotrophic lateral sclerosis (ALS). Specifically, will describe structural biological impediments, including lack an aging footprint, angiogenesis, myelination, inclusion functional immunocompetent microglia—all important factors in neurodegeneration AD, PD, ALS. Additionally, discuss technical limitations for monitoring microanatomy electrophysiology parallel, propose solutions overcome current thereby making a more tool model neurodegeneration.
Язык: Английский
Процитировано
10
Medical Review, Год журнала: 2024, Номер 4(2), С. 129 - 153
Опубликована: Март 13, 2024
Abstract In the field of biomedical research, organoids represent a remarkable advancement that has potential to revolutionize our approach studying human diseases even before clinical trials. Organoids are essentially miniature 3D models specific organs or tissues, enabling scientists investigate causes diseases, test new drugs, and explore personalized medicine within controlled laboratory setting. Over past decade, organoid technology made substantial progress, allowing researchers create highly detailed environments closely mimic body. These can be generated from various sources, including pluripotent stem cells, specialized tissue tumor cells. This versatility enables replicate wide range affecting different organ systems, effectively creating disease replicas in dish. exciting capability provided us with unprecedented insights into progression how we develop improved treatments. this paper, will provide an overview progress utilizing as preclinical models, aiding understanding providing more effective addressing diseases.
Язык: Английский
Процитировано
8
Advanced Drug Delivery Reviews, Год журнала: 2024, Номер 207, С. 115202 - 115202
Опубликована: Фев. 8, 2024
Язык: Английский
Процитировано
7
Journal of Biotechnology, Год журнала: 2024, Номер 386, С. 10 - 18
Опубликована: Март 20, 2024
Microglia are the resident macrophages in central nervous system, accounting for 10-15% of cell mass brain. Next to their physiological role development, monitoring neuronal function and maintenance homeostasis, microglia crucial brain's immune defense. Brain injury chronic neurological disorders associated with neuroinflammation, which activation is a element. acquire wide spectrum states diseased or injured brain, some neurotoxic. The investigation (patho)physiology therapeutic interventions targeting neuroinflammation substantial challenge. In addition vivo approaches, application vitro model systems has gained significant ground essential complement work. Primary cultures have proved be useful tool. offered opportunity explore mechanistic, molecular elements activation, secretome, efficacy treatments against neuroinflammation. As all systems, primary distinct strengths limitations weighed when experiments designed data interpreted. Here, we set out provide succinct overview advantages pitfalls use cultures, instructs refinement further development this technique remain toolbox researchers. Since there no conclusive therapy combat neurotoxicity linked acute brain neurodegenerative disorders, these research tools opportunities.
Язык: Английский
Процитировано
7
Acta Neuropathologica Communications, Год журнала: 2025, Номер 13(1)
Опубликована: Янв. 11, 2025
Abstract Glioblastoma (GBM) is a highly aggressive adult brain cancer, characterised by poor prognosis and dismal five-year survival rate. Despite significant knowledge gains in tumour biology, meaningful advances patient remain elusive. The field of neuro-oncology faces many disease obstacles, one being the paucity faithful models to advance preclinical research guide personalised medicine approaches. Recent technological developments have permitted maintenance, expansion cryopreservation GBM explant organoid (GBO) tissue. GBOs represent translational leap forward are currently state-of-the-art 3D vitro culture system, retaining cancer heterogeneity, transiently maintaining immune infiltrate microenvironment (TME). Here, we provide review existing technologies, vivo xenograft approaches, evaluate in-detail key advantages limitations this rapidly emerging technology, consider solutions overcome these difficulties. hold promise, with potential emerge as tool synergise enhance next-generation omics efforts approaches for patients into future.
Язык: Английский
Процитировано
1
Brain Behavior and Immunity, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
6
Inflammopharmacology, Год журнала: 2024, Номер 32(3), С. 1903 - 1928
Опубликована: Апрель 17, 2024
Penconazole (PEN) is a systemic triazole fungicide used to control various fungal diseases on grapes, stone fruits, cucurbits, and strawberries. Still, it leaves residues treated crops after collection with many hazardous effects population including neurotoxicity. Withania somnifera extract (WSLE) known for its memory brain function enhancing ability. To evoke such action efficiently, WSLE bioactive metabolites are needed cross the blood-brain barrier, that could limit availability of compounds be localized within brain. Therefore, in present study, association between PEN exposure neurotoxicity was evaluated, formulated nanoemulsion investigated improving permeability plant across barrier. The rats were divided into five groups (n = 6). group administered distilled water, II W. (WSLE NE), III received PEN, IV WSLE, V NE. All gavaged daily 6 weeks. Characterization using LC-MS/MS analysis estimated. Neurobehavioral disorders evaluated all groups. Oxidative stress biomarkers, antioxidant enzyme activities, inflammatory cytokines measured tissue. Furthermore, gene expression patterns GFAP, APP, vimentin, TGF-β1, Smad2 Bax measured. Histopathological changes immunohistochemical peripheral sciatic nerve cerebral cortex evaluated. A total 91 different chemo-types detected identified both ionization modes. Our data showed behavioral impairment PEN-treated group, significant elevation oxidative proinflammatory cytokines, neuronal damage, apoptosis. In contrast, NE marked improvement performance histopathological alteration increase activity anti-inflammatory compared alone. turn significantly downregulated levels conclusion, markedly enhanced constituents through blood barrier boost neuroprotective effect against PEN-induced
Язык: Английский
Процитировано
5
Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18
Опубликована: Май 15, 2024
Over the past two decades, Opioid Use Disorder (OUD) among pregnant women has become a major global public health concern. OUD been characterized as problematic pattern of opioid use despite adverse physical, psychological, behavioral, and or social consequences. Due to relapsing–remitting nature this disorder, mothers are chronically exposed exogenous opioids, resulting in neurological neuropsychiatric outcomes. Collateral fetal exposure opioids also precipitates severe neurodevelopmental neurocognitive sequelae. At present, much what is known regarding neurobiological consequences prenatal (POE) derived from preclinical studies animal models postnatal postmortem investigations humans. However, species-specific differences brain development, variations subject age/health/background, disparities sample collection storage have complicated interpretation findings produced by these explorations. The ethical logistical inaccessibility human tissue limited direct examinations drug effects. To circumvent confounding factors, recent groups begun employing induced pluripotent stem cell (iPSC)-derived organoid technology, which provides access key aspects cellular molecular structure, function vitro . In review, we endeavor encapsulate advancements culture that enabled scientists model dissect neural underpinnings effects POE. We hope not only emphasize utility organoids for investigating conditions, but highlight opportunities further technical conceptual progress. Although application critical field research still its nascent stages, understanding neurobiology POE via modality will provide insights improving maternal
Язык: Английский
Процитировано
4