Frontiers in Cellular Neuroscience,
Год журнала:
2025,
Номер
19
Опубликована: Апрель 7, 2025
CRISPR/Cas9
technology
has
revolutionized
genetic
and
biomedical
research
in
recent
years.
It
enables
editing
modulation
of
gene
function
with
an
unparalleled
precision
effectiveness.
Among
the
various
applications
prospects
this
technology,
opportunities
it
offers
unraveling
molecular
underpinnings
a
myriad
central
nervous
system
diseases,
including
neurodegenerative
disorders,
psychiatric
conditions,
developmental
abnormalities,
are
unprecedented.
In
review,
we
highlight
CRISPR/Cas9-based
therapeutics
as
promising
strategy
for
management
Alzheimer’s
disease
transformative
impact
on
AD
research.
Further,
emphasize
role
generating
accurate
models
identification
novel
therapeutic
targets,
besides
CRISPR-based
therapies
aimed
at
correcting
AD-associated
mutations
modulating
processes.
Furthermore,
delivery
systems
reviewed
potential
non-viral
nanotechnology-based
carriers
overcoming
critical
limitations
effective
is
discussed.
Overall,
review
highlights
promise
intricate
processes
underlying
development
AD,
discusses
its
limitations,
ethical
concerns
several
challenges
efficient
across
BBB,
ensuring
specificity,
avoiding
off-target
effects.
This
article
can
be
helpful
better
understanding
based
approaches
way
forward
utilizing
enormous
targeted,
gene-specific
treatments
that
could
change
trajectory
debilitating
incurable
illness.
Journal of Nanobiotechnology,
Год журнала:
2020,
Номер
18(1)
Опубликована: Сен. 5, 2020
Abstract
Incidents
of
viral
outbreaks
have
increased
at
an
alarming
rate
over
the
past
decades.
The
most
recent
human
coronavirus
known
as
COVID-19
(SARS-CoV-2)
has
already
spread
around
world
and
shown
R
0
values
from
2.2
to
2.68.
However,
ratio
between
mortality
number
infections
seems
be
lower
in
this
case
comparison
other
coronaviruses
(such
severe
acute
respiratory
syndrome
(SARS-CoV)
Middle
East
(MERS-CoV)).
These
tested
limits
healthcare
systems
posed
serious
questions
about
management
using
conventional
therapies
diagnostic
tools.
In
regard,
use
nanotechnology
offers
new
opportunities
for
development
novel
strategies
terms
prevention,
diagnosis
treatment
infections.
review,
we
discuss
virus
by
nano-based
materials,
such
disinfectants,
personal
protective
equipment,
nanocarrier
systems,
treatments
vaccine
development,
well
challenges
drawbacks
that
need
addressing.
Frontiers in Plant Science,
Год журнала:
2021,
Номер
12
Опубликована: Май 28, 2021
Agriculture
is
an
important
source
of
human
food.
However,
current
agricultural
practices
need
modernizing
and
strengthening
to
fulfill
the
increasing
food
requirements
growing
worldwide
population.
Genome
editing
(GE)
technology
has
been
used
produce
plants
with
improved
yields
nutritional
value
as
well
higher
resilience
herbicides,
insects,
diseases.
Several
GE
tools
have
developed
recently,
including
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
nucleases,
a
customizable
successful
method.
The
main
steps
process
involve
introducing
transgenes
or
CRISPR
into
via
specific
gene
delivery
systems.
certain
limitations,
time-consuming
complicated
protocols,
potential
tissue
damage,
DNA
incorporation
in
host
genome,
low
transformation
efficiency.
To
overcome
these
issues,
nanotechnology
emerged
groundbreaking
modern
technique.
Nanoparticle-mediated
superior
conventional
biomolecular
approaches
because
it
enhances
efficiency
for
both
temporal
(transient)
permanent
(stable)
genetic
modifications
various
plant
species.
discoveries
advanced
technologies,
challenges
developing
short-term
breeding
strategy
remain.
Thus,
this
review,
nanobased
systems
engineering
are
discussed
detail.
Moreover,
we
suggested
effective
method
hasten
crop
improvement
programs
by
combining
such
speed
CRISPR/Cas,
nanotechnology.
overall
aim
review
provide
detailed
overview
nanotechnology-based
techniques
suggest
applications
possible
enhancement.
International Journal of Nanomedicine,
Год журнала:
2022,
Номер
Volume 17, С. 3735 - 3749
Опубликована: Авг. 1, 2022
Abstract:
Cancer
remains
the
most
devastating
disease
and
major
cause
of
mortality
worldwide.
Although
early
diagnosis
treatment
are
key
approach
in
fighting
against
cancer,
available
conventional
diagnostic
therapeutic
methods
not
efficient.
Besides,
ineffective
cancer
cell
selectivity
toxicity
traditional
chemotherapy
remain
significant
challenge.
These
limitations
entail
need
for
development
both
safe
effective
options.
Due
to
its
robust
application,
nanotechnology
could
be
a
promising
method
in-vivo
imaging
detection
cells
biomarkers.
Nanotechnology
provide
quick,
safe,
cost-effective,
efficient
management.
It
also
provides
simultaneous
using
nano-theragnostic
particles
that
facilitate
selective
destruction
cells.
Updated
recent
discussions
important
selecting
best
diagnosis,
treatment,
management
options,
new
insights
on
designing
protocols
utmost
important.
This
review
discusses
application
therapeutics,
theragnosis
future
perspectives
field.
Keywords:
nanotechnology,
theragnostics
Abstract
The
CRISPR
system
is
a
revolutionary
genome
editing
tool
that
has
the
potential
to
revolutionize
field
of
cancer
research
and
therapy.
ability
precisely
target
edit
specific
genetic
mutations
drive
growth
spread
tumors
opened
up
new
possibilities
for
development
more
effective
personalized
treatments.
In
this
review,
we
will
discuss
different
CRISPR-based
strategies
have
been
proposed
therapy,
including
inactivating
genes
tumor
growth,
enhancing
immune
response
cells,
repairing
cause
cancer,
delivering
cancer-killing
molecules
directly
cells.
We
also
summarize
current
state
preclinical
studies
clinical
trials
highlighting
most
promising
results
challenges
still
need
be
overcome.
Safety
delivery
are
important
therapy
become
viable
option.
limitations
overcome,
such
as
off-target
effects,
safety,
site.
Finally,
provide
an
overview
opportunities
in
future
directions
development.
change
landscape
research,
review
aims
overcome
realize
potential.
Nanotechnology Reviews,
Год журнала:
2023,
Номер
12(1)
Опубликована: Янв. 1, 2023
Abstract
Solid
lipid
nanoparticles
(SLNs),
the
spheroidal-shaped,
colloids
state
lipophilic-natured,
innovative
nanoscale
particulate
materials,
are
being
concurrently
prepared
by
quality-by-design
approach
for
cellular
and
sub-cellular
delivery
of
drugs
other
payloads
with
facilitated
physicochemical
characteristics
targeted
delivery.
The
drugs,
pharmaceuticals
biopharmaceutical
genes
to
diseased
body
organs,
tissues,
mass
have
been
developed
as
promising
nanocarriers
different
high-incidence
cancers
disease
therapies,
including
Alzheimer’s,
Parkinson’s,
tuberculosis.
SLNs
evolved
favorable
lipid-based
formulation,
served
oral
intravenous
carriers
that
drug
stable
sterile
transport,
sustained
delivery,
controlled
drug/payload
deloading,
requisite
biodistributions.
advantages,
shortcomings,
bottlenecks
discussed
plausible
remediation
strategies.
laboratory-scale
bulk
preparations,
use
lipids
in
various
preparation,
surface
coatings,
properties
final
product,
characterization
protocols
also
encompassed,
routes
administrations,
specific-sites-targeting,
on-site
outreach
biocompatibility,
bioavailability,
absorption,
distribution,
metabolism,
excretion
pharmacokinetics,
pharmacodynamics
inputs
relevance
therapy.
Plausible
applications
complex
genetic
disorders,
personalized
medicine,
traditional
alternative
medicine
prospects,
discussed.
RGD
peptide
can
be
found
in
cell
adhesion
and
signaling
proteins,
such
as
fibronectin,
vitronectin,
fibrinogen.
peptides'
principal
function
is
to
facilitate
by
interacting
with
integrin
receptors
on
the
surface.
They
have
been
intensively
researched
for
use
biotechnology
medicine,
including
incorporation
into
biomaterials,
conjugation
medicinal
molecules
or
nanoparticles,
labeling
imaging
agents.
peptides
utilized
specifically
target
cancer
cells
tumor
vasculature
engaging
these
integrins,
improving
drug
delivery
efficiency
minimizing
adverse
effects
healthy
tissues.
RGD-functionalized
carriers
are
a
viable
option
therapy
this
focused
approach
has
demonstrated
promise
future.
Writing
review
significantly
influence
how
drugs
developed
future
our
understanding
of
peptide,
finding
knowledge
gaps,
fostering
innovation,
making
design
easier.
Journal of Advanced Research,
Год журнала:
2021,
Номер
40, С. 207 - 221
Опубликована: Июль 6, 2021
Alzheimer's
disease
(AD)
is
an
insidious,
irreversible,
and
progressive
neurodegenerative
health
condition
manifesting
as
cognitive
deficits
amyloid
beta
(Aβ)
plaques
neurofibrillary
tangles.
Approximately
50
million
individuals
are
affected
by
AD,
the
number
rapidly
increasing
globally.
This
review
explores
role
of
CRISPR/Cas9
gene
editing
in
management
AD
its
clinical
manifestations.
aims
to
provide
a
deep
insight
into
recent
progress
CRISPR/Cas9-mediated
genome
use
against
disorders,
specifically
AD.
However,
we
have
referred
parkinsons's
(PD),
Huntington's
(HD),
other
human
diseases,
one
most
promising
emerging
technologies
for
treatment.
The
pathophysiology
known
be
linked
with
mutations,
that
is,
presenilin
(PSEN)
precursor
protein
(APP).
trials
focused
at
genetic
level
could
not
meet
desired
efficiency.
tool
powerful
correcting
inconsistent
signatures
now
extensively
used
management.
It
has
significant
potential
correction
undesired
mutations
associated
technology
allowed
development
empirical
models,
therapeutic
lines,
diagnostic
approaches
better
understanding
nervous
system,
from
vitro
vivo
models.
Journal of Controlled Release,
Год журнала:
2021,
Номер
337, С. 193 - 211
Опубликована: Июль 20, 2021
Targeted
nanomaterials
for
cancer
theranostics
have
been
the
subject
of
an
expanding
volume
research
studies
in
recent
years.
Mesoporous
silica
nanoparticles
(MSNs)
are
particularly
attractive
such
applications
due
to
possibilities
synthesize
(NPs)
different
morphologies,
pore
diameters
and
arrangements,
large
surface
areas
various
options
functionalization.
Functionalization
MSNs
with
organic
inorganic
molecules,
polymers,
surface-attachment
other
NPs,
loading
entrapping
cargo
molecules
on-desire
release
capabilities,
lead
seemingly
endless
prospects
designing
advanced
nanoconstructs
exerting
multiple
functions,
as
simultaneous
cancer-targeting,
imaging
therapy.
Describing
composition
multifunctional
capabilities
these
nanoassemblies
targeted
therapy
(passive,
ligand-functionalized
MSNs,
stimuli-responsive
therapy),
including
one
or
more
modalities
tumors,
is
this
review
article,
along
overview
developments
within
a
novel
trend,
comprising
use
CRISPR/Cas9
systems
delivery
gene
editing
cancer.
Such
nanconstructs
exhibit
high
potential
image-guided
therapies
development
personalized
treatment.
Acta Biomaterialia,
Год журнала:
2022,
Номер
157, С. 1 - 23
Опубликована: Дек. 12, 2022
Pancreatic
cancer
is
one
of
the
harshest
and
most
challenging
cancers
to
treat,
often
labeled
as
incurable.
Chemotherapy
continues
be
popular
treatment
yet
yields
a
very
poor
prognosis.
The
main
barriers
such
inefficient
drug
penetration
resistance,
have
led
development
carrier
systems.
benefits,
ease
fabrication
modification
liposomes
render
them
ideal
future
delivery
This
review
delves
into
versatility
achieve
various
mechanisms
for
pancreatic
cancer.
Not
only
are
there
benefits
loading
chemotherapy
drugs
targeting
agents
onto
liposomes,
well
mRNA
combined
therapy,
but
also
been
exploited
immunotherapy
can
programmed
respond
photothermal
therapy.
Multifunctional
liposomal
formulations
demonstrated
significant
pre-clinical
success.
Functionalising
drug-encapsulated
has
resulted
in
triggered
release,
specific
targeting,
remodeling
tumor
environment.
Suppressing
progression
achieved,
due
their
ability
more
efficiently
precisely
deliver
chemotherapy.
Currently,
no
multifunctional
surface-modified
clinically
approved
thus
we
aim
shed
light
on
trials
tribulations
progress
so
far,
with
hope
therapy
improved
patient
outcomes.
STATEMENT
OF
SIGNIFICANCE:
Considering
that
conventional
treatments
highly
associated
sub-optimal
performance
systemic
toxicity,
novel
therapeutic
strategies
holds
outmost
relevance
management.
Liposomes
being
increasingly
considered
promising
nanocarriers
providing
not
an
early
diagnosis
effective,
specific,
safer
treatment,
improving
overall
outcome.
manuscript
first
last
10
years
revises
advances
application
liposome-based
bioimaging,
chemotherapy,
phototherapy,
immunotherapy,
combination
therapies,
emergent
therapies
Prospective
insights
provided
regarding
several
advantages
resulting
from
use
liposome
technology
precision
strategies,
fostering
new
ideas
next-generation
targeted
