Liquiritin inhibits H₂O₂‐induced oxidative stress injury in H9c2 cells via the AMPK/SIRT1/NF‐κB signaling pathway

Journal of Food Biochemistry, Год журнала: 2022, Номер 46(10)

Опубликована: Авг. 5, 2022

Heart failure (HF) is a serious disease with high mortality. Oxidative stress plays vital role in its occurrence and development. Licorice commonly used to treat HF traditional Chinese medicine. Liquiritin, the main ingredient of licorice, has antioxidant anti-inflammatory properties, but mechanism against oxidative cardiomyocytes not been reported. Establishment damage model H9c2 cells by hydrogen peroxide (H2 O2 ). Liquiritin (5, 10, 20 μmol/L) could significantly prevent loss cell viability decrease apoptosis rate. It can reduce levels reactive oxygen species (ROS), malonedialdehyde (MDA), lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), increase activity ATP, superoxidedismutase (SOD), glutathione (GSH-px), reductase (GR) catalase (CAT) alleviate inflammation dose-dependent manner. was found be related AMP-Activated Protein Kinase (AMPK) pathway molecular docking. Western blotting (WB) quantitative reverse transcription PCR (RT-qPCR) confirmed that liquiritin promote AMPKα phosphorylation sirtuin 1 (SIRT1) protein expression, inhibit nuclear factor kappa B p65 (NF-κB p65). Compound C, EX 527, PDTC effects liquiritin, indicating effect achieved regulating AMPK/SIRT1/NF-κB signaling pathway. PRACTICAL APPLICATIONS: one most common cardiovascular diseases, treatment remains worldwide problem. food dietary supplement widely medicine (TCM). active components which pharmacological effects. This study revealed licorice cardiomyocytes, provided scientific basis for as an heart failure.

Язык: Английский

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Liquiritin Protects Against Cardiac Fibrosis After Myocardial Infarction by Inhibiting CCL5 Expression and the NF-κB Signaling Pathway

Drug Design Development and Therapy, Год журнала: 2022, Номер Volume 16, С. 4111 - 4125

Опубликована: Дек. 1, 2022

Purpose: Despite significant advances in interventional treatment, myocardial infarction (MI) and subsequent cardiac fibrosis remain major causes of high mortality worldwide. Liquiritin (LQ) is a flavonoid extract from licorice that possesses variety pharmacological properties. However, to our knowledge, the effects LQ on after MI have not been reported detail. The aim research was explore potential role mechanism MI-induced damage. Methods: models were established by ligating left anterior descending branch coronary artery. Next, rats orally administered once day for 14 days. Biochemical assays, histopathological observations, ELISA, Western blotting analyses then conducted. Results: improved heart appearance ECG, decreased weight index reduced levels cardiac-specific markers such as CK, CK-MB, LDH, cTnI BNP. Meanwhile, infarct size hemodynamic parameters LVEDP, LVSP ±dp/dt max . Moreover, H&E staining showed attenuated pathological damage caused MI. Masson alleviated cell disorder while reducing collagen deposition. also oxidative stress inflammation. demonstrated significantly down-regulated expressions Collagen I, III, TGF-β 1, MMP-9, α-SMA, CCL5, p-NF-κB. Conclusion: protected against following improving function, attenuating inflammatory response, which may be associated with inhibition CCL5 expression NF-κB pathway. Keywords: liquiritin, fibrosis, infarction, expression, signaling pathway A Letter Editor has published this article

Язык: Английский

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Natural Product-Derived Compounds Targeting Keratinocytes and Molecular Pathways in Psoriasis Therapeutics

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6068 - 6068

Опубликована: Май 31, 2024

Psoriasis is a chronic autoimmune inflammatory skin disorder that affects approximately 2–3% of the global population due to significant genetic predisposition. It characterized by an uncontrolled growth and differentiation keratinocytes, leading formation scaly erythematous plaques. extends beyond dermatological manifestations impact joints nails often associated with systemic disorders. Although traditional treatments provide relief, their use limited potential side effects nature disease. This review aims discuss therapeutic keratinocyte-targeting natural products in psoriasis highlight efficacy safety comparison conventional treatments. comprehensively examines pathogenesis within keratinocytes various related signaling pathways (such as JAK-STAT NF-κB) cytokines. presents molecular targets such high-mobility group box-1 (HMGB1), dual-specificity phosphatase-1 (DUSP1), aryl hydrocarbon receptor (AhR) for treating psoriasis. evaluates ability compounds luteolin, piperine, glycyrrhizin modulate psoriasis-related pathways. Finally, it offers insights into alternative sustainable treatment options fewer effects.

Язык: Английский

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A comparative molecular dynamics approach guides the tailoring of glycosyltransferases to meet synthetic applications

Green Chemistry, Год журнала: 2024, Номер 26(16), С. 9186 - 9194

Опубликована: Янв. 1, 2024

The comparative molecular dynamics approach provides insights into the structural of glycosyltransferases and facilitates precise enzyme engineering for producing valuable glycosides.

Язык: Английский

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Liquiritin targeting Th17 cells differentiation and abnormal proliferation of keratinocytes alleviates psoriasis via NF‐κB and AP‐1 pathway

Phytotherapy Research, Год журнала: 2023, Номер 38(1), С. 174 - 186

Опубликована: Окт. 17, 2023

Abstract Psoriasis is a common immune‐mediated inflammatory skin disease, caused by disturbed interactions between keratinocytes and immune cells. Chinese medicine shows potential clinical application for its treatment. Liquiritin flavone compound extracted from licorice antitussive, antioxidant antiinflammatory effects, therefore may have as psoriasis therapeutic. The aim of this work was to examine the possible roles that liquiritin in treating psoriasis. HaCaT cells were stimulated TNF‐α with or without liquiritin, harvested analysis western blots RT‐qPCR, cellular supernatants collected analyzed ELISA cytokines. In addition, 4 groups mice examined: Normal, Vehicle, LQ‐L LQ‐H. sacrificed after 6 days using IHC, ELISA, RT‐qPCR flow cytometry. results showed could significantly inhibit progression both vitro vivo. strongly suppressed proliferation but did not affect cell viability. Moreover, alleviated imiquimod‐induced psoriasis‐like inflammation accumulation Th17 DCs TNF‐α‐induced keratinocytes, protein mRNA expression levels cytokines sharply decreased. mice, activation NF‐κB AP‐1 reduced treatment liquiritin. Collectively, our show might act pivotal regulator via modulating signal pathways.

Язык: Английский

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Characterization and identification of chemical components in Si-Ni-San based on UPLC-Q-Orbitrap-MS, HS-SPME-GC–MS, and HS-GC-IMS techniques

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112718 - 112718

Опубликована: Янв. 1, 2025

Язык: Английский

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Liquiritin Ameliorates Palmitic Acid‐Induced Hepatic Steatosis in Mouse Primary Hepatocytes by Suppressing the VEGFA Signaling Pathway

Journal of Food Biochemistry, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Nonalcoholic fatty liver disease (NAFLD) has become a global pandemic, imposing significant socioeconomic burden. Hepatic steatosis is key pathological event in NAFLD. However, there still lack of effective drugs for NAFLD treatment. Liquiritin (LIQ), derived from licorice root, exhibits antioxidative and anti‐inflammatory properties, making it valuable managing various conditions such as dermatological disorders, respiratory ailments, gastritis. Here, we investigated the impact underlying mechanisms LIQ on hepatic mouse primary hepatocytes (MPHs). Our study found that significantly decreased palmitic acid (PA)‐induced lipid accumulation MPHs. Additionally, remarkably increased mRNA expression Cpt1a, PPAR α , Ehhadh, Cyp4a10, Acox1, suggesting achieves its lipid‐lowering effect by regulating mitochondria‐mediated β‐oxidation. Furthermore, regulatory mitochondrial oxidative phosphorylation (OXPHOS) process was confirmed Seahorse analysis. Mechanistically, bioinformatics analysis predicted VEGFA crucial target mediating beneficial effects against PA‐induced Notably, treatment with purified protein partially counteracted properties LIQ. our data showed promoted degradation through ubiquitin–proteasome pathway. Therefore, findings not only confirm MPHs but also identify potential target. These results highlight therapeutic promise introduce novel treating steatosis.

Язык: Английский

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Crystalline Liquiritigenin and Liquiritin: Structural Characterization, Molecular Docking Studies, and Anti-Amyloid-β Evaluation in Caenorhabditis elegans

ACS Omega, Год журнала: 2025, Номер 10(7), С. 7112 - 7119

Опубликована: Фев. 16, 2025

Two new crystalline compounds, named [LG·H2O]n (1; LG = liquiritigenin) and [LQ·C2H5OH·H2O]n (2; LQ liquiritin), have been synthesized structurally characterized by single-crystal powder X-ray diffraction, thermogravimetric analyses (TGA), nuclear magnetic resonance (NMR), high-resolution mass spectrometry (HR-MS), infrared spectra (IR). 1 2 crystallize in space groups Pna21 P212121, respectively. In the structure of 1, liquiritigenin water molecules are connected hydrogen bonds for construction a novel 3,5-connected network topology with point symbol (63)(67·83), which each molecule acts as 5-connected 3-connected node, Both reduce amyloid-β-induced toxicity Caenorhabditis elegans (CL4176 strain) improving expression level SOD. Gene studies RT-qPCR indicate upregulation skn-1 sod-3 while downregulation daf-16 hsf-1 C. elegans. Molecular docking that combine well vascular endothelial growth factor A (VEGFA), free binding energies calculated to be −6.7 −7.9 kcal·mol–1, Moreover, anti-amyloid-β ability amorphous or has studied.

Язык: Английский

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Research Progress of Traditional Chinese Medicine in the Treatment of Gastric Cancer Based on Different Signaling Pathways

Advances in Clinical Medicine, Год журнала: 2025, Номер 15(04), С. 2136 - 2143

Опубликована: Янв. 1, 2025

Язык: Английский

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Precision Strike Strategy for Liver Diseases Trilogy with Xiao-Chai-Hu Decoction: A Meta-Analysis with Machine Learning

Phytomedicine, Год журнала: 2025, Номер 142, С. 156796 - 156796

Опубликована: Апрель 23, 2025

Язык: Английский

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