Natural Product Communications,
Год журнала:
2023,
Номер
18(11)
Опубликована: Ноя. 1, 2023
Introduction
Cladonia
rangiformis,
also
known
as
reindeer
lichen,
has
been
used
for
various
remedies
in
folk
medicine.
The
rich
nutritional
value
of
this
lichen
is
reflected
the
fact
that
it
contains
atranorin,
which
proven
to
have
numerous
biological
activities.
Objective
chemical
composition,
antioxidant,
anticholinesterase,
and
antigenotoxic
activities
C.
rangiformis
acetone
extract
were
investigated.
novelty
work
anticholinesterase
activity,
protective
effect
on
human
lymphocytes,
antioxidant
activity
by
cupric-reducing
capacity
(CUPRAC)
method
determined
first
time,
well
gas
chromatography
-
mass
spectrometry
(GC-MS)
profile.
Methods
Chemical
composition
was
carried
out
high-performance
liquid
(HPLC)-diode
array
detector
GC-MS.
Antigenotoxic
evaluated
lymphocytes
cytokinesis-block
micronucleus
assay.
Total
phenolic
content,
DPPH
ABTS
radical
scavenging
capacities,
CUPRAC,
total
reducing
power
spectrophotometrically.
Determination
acetylcholinesterase
performed
Ellman's
colorimetry
statistical
analysis
variance
(One-way
ANOVA)
using
Origin
software
package
version
7.0.
Results
HPLC
identified
3
compounds
with
atranorin
predominant
constituent
(97.2%).
Fumarprotocetraric
atraric
acid
represented
a
much
smaller
mutually
approximate
amount,
0.3
0.5%,
respectively.
Rangiformic
most
abundant
volatile
compound.
Acetone
(2
μg/mL)
decreased
frequency
micronuclei
15.5%
while
radioprotectant
amifostine
reduced
occurrence
11.4%.
On
contrary,
at
concentration
1.0
mg/mL
exhibited
an
activating
cholinesterase
(2.0%),
10.0
showed
weak
inhibitory
(7.3%).
phenol
content
132.71
μg
gallic
equivalents
per
mg
dry
extract.
IC
50
experiment
16.19
11.80
mg/mL.
Cupric
17.81
Trolox
0.45
ascorbic
extract,
Conclusion
In
addition
previously
time
method,
orcinol,
β-orcinol,
lauric
alcohol,
atranol,
rangiformic
GC-MS
method.
results
proved
had
activity.
Reduction
number
classified
promising
substances
source
beneficial
effects
DNA
damage.
Molecules,
Год журнала:
2023,
Номер
28(23), С. 7840 - 7840
Опубликована: Ноя. 29, 2023
The
present
study
was
intended
for
the
identification
of
secondary
metabolites
in
acetone
extract
lichen
Hypotrachyna
cirrhata
using
UPLC-ESI-QToF-MS/MS
and
detection
bioactive
compounds.
This
led
to
22
based
on
their
MS/MS
spectra,
accurate
molecular
masses,
formula
from
a
comparison
literature
database
(DNP),
fragmentation
patterns.
In
addition,
potent
antioxidant
α-glucosidase
inhibitory
potentials
H.
motivated
us
isolate
10
metabolites,
which
were
characterized
as
salazinic
acid
(11),
norlobaridone
(12),
atranorin
(13),
lecanoric
(14),
lichesterinic
(15),
protolichesterinic
(16),
methyl
hematommate
(17),
iso-rhizonic
(18),
atranol
(19),
methylatratate
(20)
spectral
data.
All
these
isolates
assessed
free
radicals
scavenging,
radical-induced
DNA
damage,
intestinal
activities.
results
indicated
that
(19)
showed
activity,
while
depsidones
(salazinic
(12))
monophenolic
compound
(iso-rhizonic
acid,
(18))
displayed
significant
activities
(p
<
0.001),
is
comparable
standard
acarbose.
These
further
correlated
with
docking
studies,
alkyl
chain
norlobaridione
(12)
hooked
into
finger-like
cavity
allosteric
pocket;
moreover,
it
also
established
Van
der
Waals
interactions
hydrophobic
residues
pocket.
Thus,
potency
inhibit
enzyme
might
be
associated
its
binding.
Also,
MM-GBSA
(Molecular
Mechanics-Generalized
Born
Surface
Area)
binding
energies
(11)
superior
acarbose
may
have
contributed
high
activity
compared
Drug and Chemical Toxicology,
Год журнала:
2024,
Номер
unknown, С. 1 - 14
Опубликована: Сен. 25, 2024
In
our
study,
the
antioxidant
and
anti-inflammatory
effects
of
different
lichen
applications
were
investigated
in
rats
using
an
experimental
ethanol
toxicity
model.
48
used
study
they
divided
into
6
groups
with
8
each
group.
These
were:
control,
(2
g/kg),
+
Eurasian Journal of Medicine,
Год журнала:
2023,
Номер
54(Supp1), С. S195 - S208
Опубликована: Янв. 18, 2023
Lichens
are
a
unique
group
of
organisms,
which
can
produce
compounds
that
named
secondary
metabolites
and
rarely
or
not
produced
in
other
organisms.
possess
pharmacological
actions
related
to
their
metabolites.
Our
knowledge
lichens
rapidly
increases
as
new
technologies
devices,
facilitate
the
investigation
chemical
profile
biological
activities
lichens,
introduced
become
more
readily
available.
In
addition,
methods
perspectives,
well
suggestions
for
mechanisms,
accumulate
daily.
Furthermore,
lichen
substances
stand
relatively
untapped
source
natural
products.
Accordingly,
researchers
investigate
lichen-derived
material
frequently
than
it
was
past.
This
review
focused
on
published
last
11
years
(2012-2022).
Literature
data
obtained
from
WebOfScience
PubMed
databases
using
search
keywords
revealed
anti-genotoxicity,
anticancer,
anti-microbial
activity
studies
have
constantly
been
conducted.
More
recently,
immunomodulatory
inflammation-related
took
stage.
Enzyme
inhibition
were
popular
well.
selection
based
novelty
mechanistic
insight
papers
presented.
Applied Sciences,
Год журнала:
2023,
Номер
13(3), С. 1976 - 1976
Опубликована: Фев. 3, 2023
The
antimicrobial
properties
of
two
lichen
extracts
(LC1
and
LC2
solutions
extracted
in
acetone
cyclohexane,
respectively)
were
investigated
against
both
Gram-positive
Gram-negative
microorganisms
through
the
agar
well
diffusion
assay.
Results
displayed
that
samples
similarly
effective
all
indicator
strains.
activity
was
maintained
up
to
30
days
Candida
albicans
ATCC
10231
with
an
inhibition
zone
38
mm
37
for
LC1
extracted,
respectively.
In
order
separate
single
chemical
components
associate
them
biological
activity,
subjected
activity-guided
fractionation
followed
by
a
liquid
chromatography
mass
spectrometry
(LC–MS)
Ion
Trap
6310A
characterization.
Chromatogram
analysis
each
sample
revealed
presence
significant
peak,
at
retention
time
(tR)
10.8
min,
corresponding
scabrosin
derivative
could
likely
be
associated
activity.
obtained
present
investigation,
especially
opportunistic
pathogen
C.
albicans,
are
encouraging
represent
preliminary
step
future
solution
toward
microorganism
responsible
fungal
infections,
mainly
occurring
immunocompromised
patients
recently
caused
drug-resistant
Ecological Engineering & Environmental Technology,
Год журнала:
2023,
Номер
24(9), С. 183 - 190
Опубликована: Окт. 11, 2023
The
study
was
aimed
to
evaluate
the
impact
of
extraction
solvent
on
phenolic
content,
total
flavonoids
and
antioxidant
activities
acetonic,
methanolic,
aqueous
extracts
two
lichen
species:
Evernia
prunastri
Ramalina
lacera
collected
from
trunks
Argania
spinosa
using
ultrasound
assistance
extraction.Various
in
vitro
assays
were
utilized
such
as
2,2-diphenly-picryly-hydroxyl
free
radical
(DPPH)
assay,
ferric
reducing
power
(FRAP)
assay.All
tested
samples
exhibited
a
good
activity,
for
DPPH
inhibition
percentage
ranged
85±0.2%
27±0.01%,
content
13.17±0.5mgGAE/g
DW
3.31±0.3mgGAW/g
DW,
5.84±0.03mgRE/g
0.01±0.03mgRE/g
DW.This
demonstrates
that
has
significant
influence
lichens
compounds
their
also
showed
contents
are
significantly
correlated
activity
studies
lichens;
moreover,
it
shows
method
extract
compound.This
suggests
could
be
natural
source.
Natural Product Research,
Год журнала:
2024,
Номер
unknown, С. 1 - 14
Опубликована: Май 30, 2024
Lichens
contain
different
types
of
chemical
compounds
with
multiple
biological
activities
that
demonstrate
their
potential
pharmacological
use.
This
research
aims
to
report
the
metabolomic
identification
ethanolic
extracts
P.
antarcticum
and
hypnorum,
antioxidant,
enzyme
inhibitory,
cytoprotection
activity.
Sixteen
metabolites
were
identified
in
twelve
hypnorum;
reported
variable
antioxidant
activity
IC50
>350
µg/mL
DPPH·,
values
>18
µmol
Trolox/g
ORAC
>40
FRAP
a
phenolic
compound
content
>10
mg
GAE/g,
as
well
significant
results
cholinesterases,
α-glucosidase,
pancreatic
lipase,
α-amylase,
tyrosinase
inhibition
ranging
from
18
510
µg/mL,
which
complemented
by
molecular
docking
experiments.
Both
showed
improved
at
concentrations
0.5
1.0
μg/mL.
study
contributes
knowledge
diversity
Antarctic
lichen
effectiveness
evaluation
related
neurodegenerative
diseases
metabolic
syndrome.
Lichen
taxonomy
is
a
dynamic
and
ever-changing
field
that
critical
to
understanding
lichen
variety,
distribution,
ecological
relevance.
This
chapter
presents
comprehensive
review
of
the
current
state
taxonomy,
highlighting
challenges
advancements
in
species
identification,
classification,
nomenclature,
molecular
approaches.
Various
methodologies
employed
are
discussed,
including
morphological
examination,
chemical
analysis,
techniques.
Traditional
approaches
involving
detailed
examination
thallus
characteristics,
reproductive
structures,
secondary
metabolite
production
remain
crucial
for
identification.
Chemical
analyses,
such
as
thin-layer
chromatography
spot
tests,
provide
valuable
information
on
profiles
aid
delimitation.
Furthermore,
techniques,
DNA
barcoding,
have
revolutionized
by
providing
insights
into
phylogenetic
relationships
genetic
diversity
lichens.
The
manuscript
highlights
recent
integration
multi-locus
data
application
methods
resolving
complex
taxonomic
groups.
These
resulted
discovery
cryptic
species,
revisions,
description
new
taxa.
Additionally,
discusses
importance
standardized
nomenclature
following
International
Code
Nomenclature
algae,
fungi,
plants
(ICN).
It
emphasizes
need
collaboration
among
taxonomists
open-access
databases
online
resources
disseminating
information.
In
conclusion,
this
provides
overview
its
importance,
methodologies,
advancements,
challenges.
serves
resource
researchers,
taxonomists,
conservationists
working
with
lichens
underscores
role
conserving
diversity.
