Molecules,
Год журнала:
2024,
Номер
29(5), С. 1082 - 1082
Опубликована: Фев. 29, 2024
Enhalus
arcoides
is
a
highly
beneficial
type
of
seagrass.
Prior
studies
have
presented
proof
the
bioactivity
E.
acoroides,
suggesting
its
potential
to
combat
cancer.
Therefore,
this
study
aims
delve
deeper
into
acoroides
bioactive
molecule
profiles
and
their
direct
biological
anticancer
activities
potentials
through
combination
in-silico
in-vitro
studies.
This
conducted
metabolite
profile
analysis
on
utilizing
HPLC-ESI-HRMS/MS
analysis.
Two
extraction
techniques,
ethanol
hexane,
were
employed
for
process.
Furthermore,
was
using
molecular
docking
simulations
HER2,
EGFR
tyrosine
kinase
HIF-1α
protein
receptor.
Afterward,
antioxidant
activity
metabolites
examined
ABTS,
antiproliferative
tested
an
MTT
assay.
An
revealed
ability
breast
cancer
by
inhibiting
HER2/EGFR/HIF-1α
pathway
docking.
In
addition,
assay
demonstrated
that
higher
dosages
from
increased
effectiveness
toxicity
against
cell
lines.
Additionally,
possess
function
as
potent
antioxidants,
effectively
series
carcinogenic
mechanisms.
Ultimately,
showed
new
approach
unveiling
metabolites’
receptors,
with
great
cytotoxicity
property
prevent
cascade.
Molecules,
Год журнала:
2023,
Номер
28(20), С. 7037 - 7037
Опубликована: Окт. 11, 2023
Coffee
became
a
beverage
that
was
in
demand
the
world
and
consequently
produced
millions
of
tons
coffee
byproducts
namely
silverskin
(CS).
Unutilized
CS
will
be
waste
cause
environmental
pollution
such
as
greenhouse
gas
emissions,
landfill
waste,
groundwater
contamination.
This
is
research
concern
at
this
time,
although
many
studies
have
been
conducted
to
find
newer
applications
CS,
exploration
its
benefits
health
sector
still
limited.
Therefore,
exploring
prevent
or
delay
aging
very
interesting
develop
functional
food
industry
technology.
study
aims
report
profiling
metabolites
phytochemicals,
biological
activities
terms
antioxidant
activity,
potential
anti-aging
via
molecular
docking
simulation
vitro
modulation
mTOR/AMPK/SIRT1
pathway.
Something
new
has
obtained
from
work,
profile
phytocompounds,
both
studies.
Some
compounds
observed
Robusta
extract
(rCSE)
Epicatechin,
Kaempferol,
Quercitrin,
Arabica
(aCSE)
(+)-Catechin
dan
Naringin
promising
inhibitors
iNOS,
mTOR,
HIF-1α
simulation.
Interestingly,
activity
assay
rCSE
showed
same
results
More
interestingly,
AMPK/SIRT1/mTOR
expressions
are
well
modulated
by
compared
aCSE
significantly
(p
<
0.05).
makes
candidate
for
development
and/or
treatment
agent
combating
free
radicals
process.
In
vivo
human
trials
certainly
needed
see
further
efficacy
future.
Future Foods,
Год журнала:
2024,
Номер
9, С. 100354 - 100354
Опубликована: Апрель 23, 2024
Novel
dietary
strategies
are
urgently
needed
to
address
the
limitations
of
current
management
and
treatment
options
Type-2
Diabetes
(T2D).
Marine
algae-derived
peptides
(MAP)
represent
a
promising
avenue,
although,
their
potential
remains
mostly
underexplored.
This
study
employs
pharmacoinformatics
in
vitro
methods
evaluate
antidiabetic
properties
MAP
provide
new
insights
mechanisms
mitigate
prevalence
T2D.
Through
systematic
search
predictive
modeling,
were
identified
assessed
for
bioactivity,
toxicity,
drug-likeness.
Furthermore,
molecular
docking
simulations
with
protein
targets
related
T2D
binding
sites
that
be
used
optimize
activity
MAP.
The
structure-activity
relationship
profile
reveals
13
candidates
probable
(Pa)
scores
>0.4,
indicative
insulin
promoter.
peptide
FDGIP
(P13;Phe-Asp-Gly-Ile-Pro)
from
Caulerpa
lentillifera
had
best
silico
assessment
value
compared
50
other
its
was
confirmed
by
data
(e.g.EC50
60.4
57.9
α-amylase
α-glucosidase
inhibitions).
Interestingly,
3T3-L1
cells,
P13
exhibited
inhibitory
activities
against
transcription
factors
hormones
(MAPK8-JNK1/PPARGC1A/Ghrelin/GLP-1/CPT-1)
can
regulate
blood
sugar
decrease
as
anti-diabetes.
then
appears
action
may
formulation
foods
manage
Marine Drugs,
Год журнала:
2024,
Номер
22(8), С. 365 - 365
Опубликована: Авг. 12, 2024
Enhalus
acoroides,
a
tropical
seagrass,
is
known
for
its
significant
contribution
to
marine
ecosystems
and
potential
health
benefits
due
bioactive
compounds.
This
study
aims
compare
the
carotenoid
levels
in
E.
acoroides
using
green
extraction
via
ultrasound-assisted
(UAE)
microwave-assisted
(MAE)
evaluate
biological
properties
of
these
extracts
against
oxidative
stress,
diabetes,
obesity
through
silico
vitro
analyses.
samples
were
collected
from
Manado
City,
Indonesia,
subjected
UAE
MAE.
The
analyzed
UHPLC-ESI-MS/MS
identify
carotenoids,
including
β-carotene,
lutein,
lycopene,
β-cryptoxanthin,
zeaxanthin.
In
analysis
was
conducted
predict
compounds’
bioactivity,
toxicity,
drug-likeness
WAY2DRUG
PASS
molecular
docking
with
CB-Dock2.
compounds
C3,
C4,
C7
demonstrated
notable
interactions,
key
metabolic
proteins
microRNAs,
further
validating
their
therapeutic
benefits.
assays
evaluated
antioxidant
activities
DPPH
FRAP
assays,
antidiabetic
α-glucosidase
α-amylase
inhibition,
antiobesity
effects
lipase
inhibition
MTT
assay
3T3-L1
cells.
Results
indicated
that
both
MAE
exhibited
antioxidant,
antidiabetic,
activities.
showed
higher
content
greater
activity
compared
extracts.
These
findings
suggest
mainly
when
extracted
MAE,
has
promising
as
source
natural
developing
marine-based
agents.
supplements
existing
literature
by
providing
insights
into
efficient
methods
carotenoids.
Molecules,
Год журнала:
2023,
Номер
28(13), С. 5076 - 5076
Опубликована: Июнь 28, 2023
The
roots
of
Peucedanum
praeruptorum
Dunn
and
Angelica
decursiva
Franchet
et
Savatier
are
designated
Zenko,
which
is
a
crude
drug
defined
by
the
Japanese
Pharmacopoeia.
This
used
as
an
antitussive
expectorant
included
in
Kampo
formula
Jinsoin,
improves
cough,
fever,
headache.
Although
anti-inflammatory
effects
this
have
been
determined,
constituents
responsible
for
effect
remain
unknown.
To
investigate
biologically
active
compounds,
rat
hepatocytes
were
used,
produce
proinflammatory
mediator
nitric
oxide
(NO)
response
to
cytokine
interleukin
1β
(IL-1β).
A
methanol
extract
P.
roots,
suppressed
IL-1β-induced
NO
production,
was
fractionated
into
three
fractions
(ethyl
acetate
(EtOAc)-soluble,
n-butanol-soluble,
water-soluble
fractions)
based
on
hydrophobicity.
EtOAc-soluble
fraction
markedly
inhibited
production.
After
purified,
compounds
identified
praeruptorins
A,
B,
E,
contents
high.
comparison
their
activities
indicated
that
praeruptorin
B
exhibited
highest
potency
inhibit
production
decreasing
inducible
synthase
expression
mRNAs
encoding
cytokines.
Collectively,
may
primarily
contribute
roots.
Nutrients,
Год журнала:
2024,
Номер
16(10), С. 1441 - 1441
Опубликована: Май 10, 2024
Metabolic
syndrome
is
a
global
health
problem.
The
use
of
functional
foods
as
dietary
components
has
been
increasing.
One
food
interest
forest
onion
extract
(FOE).
This
study
aimed
to
investigate
the
effect
FOE
on
lipid
and
glucose
metabolism
in
silico
vitro
using
3T3-L1
mouse
cell
line.
was
comprehensive
that
used
multi-modal
computational
network
pharmacology
analysis
molecular
docking
cells
vitro.
phytochemical
were
analyzed
untargeted
ultra-performance
liquid
chromatography–tandem
mass
spectrometry
(UPLC-MS).
Next,
an
performed
determine
FOE’s
bioactive
compounds,
toxicity
analysis,
protein
target
identification,
pharmacology,
carried
out.
pancreatic
lipase,
α-glucosidase,
α-amylase
inhibition
determined.
Finally,
we
determined
its
accumulation
MAPK8,
PPARG,
HMGCR,
CPT-1,
GLP1
expression
preadipocyte
We
showed
potential
metabolites
targeted
inhibited
lipase
levels,
Furthermore,
significantly
(p
<
0.05)
inhibits
expressions
GLP-1
dose-dependent
manner.
contains
several
reduce
α-amylase,
proteins
associated
with
Journal of Agriculture and Food Research,
Год журнала:
2024,
Номер
18, С. 101334 - 101334
Опубликована: Июль 30, 2024
Functional
foods
containing
antioxidant
molecules
can
limit
the
prevalence
of
various
diseases,
increase
immunity,
slow
aging,
and
improve
physical
appearance.
The
aim
this
study
was
to
use
non-toxic
prepare
extracts
from
Sonneratia
alba
fruit,
determine
phytochemical
composition,
assess
biological
activity
via
in
silico
vitro
capability.
Variations
temperatures
times
showed
that
conditions
for
best
phytochemicals
were
100
°C
10
min.
highest
values
23.33
mg
quercetin
equivalent/g
(flavonoids),
1.24
tannic
acid
(tannins),
IC50
=
62.38
ppm
(antioxidant
activity).
Gas
chromatography
displayed
7
peaks
attributed
21
possible
compounds.
Molecular
docking
analysis
results
mechanism
three
compounds
(1)
Ethyl
iso-allocholate,
(2)
Gibb-3-ene-1,10-dicarboxylic
acid,2,4a,7-trihydroxy-1-methyl-8-methylene-,1,4a-lactone,10-methyl
ester,(1a,2β,4aa,4bβ,10β)-,
(3)
Pregn-4-ene-3,20-dione,17,21-dihydroxy-,bis(O-methyloxime)
could
be
due
their
binding
hydrogen
bonding
3
reactive
oxygen
species
(ROS)
proteins
(nitric
oxide
synthases).
Subcritical
water
extract
Mangrove
fruit
(S.
alba)
has
potential
as
a
functional
food
vitro.
Nutrients,
Год журнала:
2023,
Номер
15(18), С. 3886 - 3886
Опубликована: Сен. 6, 2023
Metabolic
dysfunction,
which
includes
intra-abdominal
adiposity,
glucose
intolerance,
insulin
resistance,
dyslipidemia,
and
hypertension,
manifests
into
metabolic
syndrome
related
diseases.
Therefore,
the
discovery
of
new
therapies
in
fight
against
is
very
challenging.
This
study
aims
to
reveal
existence
an
edible
bird
nest
(EBN)
as
a
functional
food
candidate
that
may
be
alternative
fighting
syndrome.
The
included
three
approaches:
silico
molecular
docking
simulation,
vitro,
vivo
rats
fed
on
cholesterol-
fat-enriched
diets.
Four
terpenoids
Bakuchiol,
Curculigosaponin
A,
Dehydrolindestrenolide,
1-methyl-3-(1-methyl-ethyl)-benzene
EBN
have
been
identified
through
LCMS/MS-QTOF.
In
simulations,
Bakuchiol
Dehydrolindestrenolide
are
considered
potent
because
they
higher
inhibitory
power
four
receptors
(iNOS,
ROS1
kinase,
FTO,
lipase)
than
standard
drugs.
vitro
tests
also
provide
insight
antioxidant,
antidiabetic,
antiobesity
activities
EBN,
quite
feasible
due
smaller
EC50
value
compared
Interestingly,
studies
showed
significant
improvements
(p
<
0.05)
lipid
profile,
blood
glucose,
enzymatic
levels,
inflammatory
biomarkers
given
high-dose
dietary
supplementation
EBN.
More
interestingly,
upregulates
PGC-1α
downregulates
HMG-CoA
reductase.
Comprehensively,
it
has
revealed
can
novel
foods
for
combating
