Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 10, 2023
Abstract
The
cell-derived
vesicles
(CDVs)
obtained
using
a
proprietary
extrusion
process
are
the
foundation
of
BioDrone™
platform
technology.
With
superior
productivity
and
versatility,
this
technology
has
garnered
increasing
attention
in
broad
applications,
particularly
as
drug
delivery
vehicle.
Previously,
we
showed
that
CDVs
exhibited
varying
levels
expression
for
tetraspanin
organelle
membrane
markers
while
revealing
no
discernible
differences
physical
characteristics
compared
to
naturally
produced
extracellular
(EVs).
To
further
understand
utilize
therapeutic
potentials
CDVs,
more
comprehensive
study
protein
profiles
is
necessary.
In
addition,
it
crucial
validate
from
indeed
intact
lipid
rather
than
other
impurities.
Here,
multiple
batches
EVs
HEK293
cells.
were
subjected
same
purification
processes
subsequent
proteome
particle
analyses.
analyses
revealed
unique
signatures
between
EVs,
parental
Extensive
identified
nine
most
prominent
abundant
cells
EVs.
Subsequent
western
blotting
nanoparticle
flow
cytometry
confirmed
CD63,
lysosome-associated
glycoprotein
1
(LAMP1),
nicastrin
(NCSTN)
highly
enriched
whereas
CD81,
CD9,
prostaglandin
F2
receptor
negative
regulator
(PTGFRN)
This
highlights
composition
marker
signature
distinct
Lastly,
demonstrated
90%
genuine
by
combining
two
different
classes
vesicle
labeling
dyes
detergents
disrupt
membranes.
indicates
our
compatible
with
well-characterized
EV
production
methods.
robust
CDV
will
also
facilitate
engineering
achieve
enhanced
effects
or
tissue-selective
cargo
delivery.
Abstract
Cancer
immunotherapy,
which
harnesses
the
power
of
immune
system,
has
shown
immense
promise
in
fight
against
malignancies.
Messenger
RNA
(mRNA)
stands
as
a
versatile
instrument
this
context,
with
its
capacity
to
encode
tumor-associated
antigens
(TAAs),
cell
receptors,
cytokines,
and
antibodies.
Nevertheless,
inherent
structural
instability
mRNA
requires
development
effective
delivery
systems.
Lipid
nanoparticles
(LNPs)
have
emerged
significant
candidates
for
cancer
providing
both
protection
enhanced
intracellular
efficiency.
In
review,
we
offer
comprehensive
summary
recent
advancements
LNP-based
systems,
focus
on
strategies
optimizing
design
mRNA-encoded
therapeutics
treatment.
Furthermore,
delve
into
challenges
encountered
field
contemplate
future
perspectives,
aiming
improve
safety
efficacy
immunotherapies.
Graphical
Biomaterials Research,
Год журнала:
2023,
Номер
27(1)
Опубликована: Фев. 9, 2023
Extracellular
vesicles
(EVs)
are
nanosized
particles
that
released
from
cells
and
reflect
the
characteristics
of
mother
cell.
Recently,
EVs
have
been
used
in
several
types
studies
across
many
different
fields.
In
field
EV
research,
multiple
cell
culture
isolation
techniques
highlighted
importance.
Various
strategies,
including
exclusive
component
media,
three-dimensional
(3D)
cultures,
hypoxic
conditions,
proposed
for
to
control
function
EVs.
Ultracentrifugation,
ultrafiltration,
precipitation,
tangential
flow
filtration
(TFF)
utilized
isolation.
Although
isolated
their
own
functionalities,
researchers
trying
functionalize
by
applying
various
engineering
approaches.
Gene
editing,
exogenous,
endogenous,
hybridization
methods
four
well-known
functionalization
strategies.
engineered
through
these
processes
has
applied
regenerative
medicine,
kidney
diseases,
osteoarthritis,
rheumatoid
arthritis,
nervous
system-related
others.
this
review,
it
was
focused
on
approaches
applications
medicine.
Journal of Nanotheranostics,
Год журнала:
2023,
Номер
4(3), С. 299 - 322
Опубликована: Июль 24, 2023
The
introduction
of
cancer
therapeutics
and
nanotechnology
has
resulted
in
a
paradigm
shift
from
conventional
therapy
to
precision
medicine.
Nanotechnology,
an
interdisciplinary
field
with
focus
on
biomedical
applications,
holds
immense
promise
bringing
about
novel
approaches
for
detection,
diagnosis,
therapy.
past
decade
witnessed
significant
research
material
applications
related
nanoparticles
(NPs).
NPs
differ
small-molecule
drugs
as
they
possess
unique
physicochemical
characteristics,
such
large
surface-to-volume
ratio,
enabling
them
penetrate
live
cells
efficiently.
Traditional
therapies,
chemotherapy,
radiation
therapy,
targeted
immunotherapy,
have
limitations,
cytotoxicity,
lack
specificity,
multiple
drug
resistance,
which
pose
challenges
effective
treatment.
However,
nanomaterials
properties
that
enable
new
therapeutic
modalities
beyond
delivery
the
fight
against
cancer.
Moreover,
(1–100
nm)
numerous
benefits,
biocompatibility,
reduced
toxicity,
excellent
stability,
enhanced
permeability
retention
effect,
precise
targeting,
making
ideal
purpose
this
article
is
provide
consolidated
information
various
bio-inspired
aid
theranostics.
Acta Pharmaceutica Sinica B,
Год журнала:
2024,
Номер
14(7), С. 3169 - 3183
Опубликована: Март 7, 2024
Optimum
genetic
delivery
for
modulating
target
genes
to
diseased
tissue
is
a
major
obstacle
profitable
gene
therapy.
Lipid
nanoparticles
(LNPs),
considered
prospective
vehicle
nucleic
acid
delivery,
have
demonstrated
efficacy
in
human
use
during
the
COVID-19
pandemic.
This
study
introduces
novel
biomaterial-based
platform,
M1-polarized
macrophage-derived
cellular
nanovesicle-coated
LNPs
(M1-C-LNPs),
specifically
engineered
combined
gene-immunotherapy
approach
against
solid
tumor.
The
dual-function
system
of
M1-C-LNPs
encapsulates
Bcl2-targeting
siRNA
within
and
immune-modulating
cytokines
M1
nanovesicles
(M1-NVs),
effectively
facilitating
apoptosis
cancer
cells
without
impacting
T
NK
cells,
which
activate
intratumoral
immune
response
promote
granule-mediating
killing
tumor
eradication.
Enhanced
retention
was
observed
upon
administration
M1-C-LNPs,
owing
presence
adhesion
molecules
on
M1-NVs,
thereby
contributing
superior
growth
inhibition.
These
findings
represent
promising
strategy
development
targeted
effective
nanoparticle-based
genetic-immunotherapy,
with
significant
implications
advancing
biomaterial
therapeutics.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(51), С. 59199 - 59208
Опубликована: Ноя. 20, 2023
Particulate
matter
(PM)
exposure
disrupts
the
skin
barrier,
causing
cutaneous
inflammation
that
may
eventually
contribute
to
development
of
various
diseases.
Herein,
we
introduce
anti-inflammatory
artificial
extracellular
vesicles
(AEVs)
fabricated
through
cell
extrusion
using
biosurfactant
PEGylated
mannosylerythritol
lipid
(P-MEL),
hereafter
named
AEVP-MEL.
The
P-MEL
has
abilities
with
demonstrated
efficacy
in
inhibiting
secretion
pro-inflammatory
mediators.
Mechanistically,
AEVP-MEL
enhanced
response
by
mitogen-activated
protein
kinase
(MAPK)
pathway
and
decreasing
release
inflammatory
mediators
such
as
reactive
oxygen
species
(ROS),
cyclooxygenase-2
(COX-2),
cytokines
human
keratinocytes.
Moreover,
promoted
increased
expression
levels
barrier
proteins
(e.g.,
involucrin,
IVL)
water–proteins
aquaporin
3,
AQP3).
In
vivo
studies
revealed
repeated
PM
intact
resulted
responses,
including
thickness
(hyperkeratosis)
mast
infiltration.
Importantly,
our
data
showed
treatment
significantly
restored
immune
homeostasis
affected
PM-induced
intrinsic
function.
This
study
highlights
potential
promoting
health
against
its
promising
implications
for
prevention
PM-related
disorders.
Extracellular
vesicles
(EVs)
possess
the
characteristics
of
their
parent
cells,
based
on
which
various
studies
have
actively
investigated
treatments
for
diseases
using
mesenchymal
stem
cell-derived
EVs
due
to
regenerative
activity.
Furthermore,
in
recent
years,
there
been
significant
efforts
engineer
improve
native
activities
and
integrate
additional
functions.
Although
both
endogenous
exogenous
methods
are
used
engineering
EVs,
may
pose
problem
administering
substances
cells
undergoing
metabolic
changes,
can
cause
potential
side
effects.
In
addition,
limitation
losing
beneficial
factors
inside
membrane
disruption
during
processes.
Surface
modification
also
impair
efficiency
presence
proteins
EV
surface.
Therefore,
this
study,
a
stable
efficient
method
was
achieved
through
ethanol-mediated
hybridization
functionalized
lipid
nanoparticles
(LNPs)
with
fusogenic
component.
During
hybridization,
internal
bioactive
targeting
moiety
were
maintained
LNPs
EVs.
The
Ab-Hybrid,
successfully
synthesized
nicotinamide-encapsulated
Col2A1
antibody-modified
liposome
Transforming
growth
factor-β1
(TGF-β1)-overexpressed
administered
osteoarthritis
(OA)-induced
rats
destabilization
medial
meniscus
surgery.
Ultimately,
Ab-Hybrid
demonstrated
excellent
chondroprotective
anti-inflammatory
effects
long-lasting
properties
OA
lesions.
We
anticipate
that
approach
manufacturing
hybrid
particles
will
serve
as
valuable
versatile
platform
technology
applicable
diseases.
Abstract
Although
immunotherapy
demonstrates
considerable
prospect
in
overcoming
solid
tumors,
its
clinical
efficacy
is
limited
by
several
factors,
such
as
poor
tumor
immunogenicity,
inadequate
immune
activation,
and
immunosuppressive
microenvironment
(TME).
To
overcome
these
challenges,
a
versatile
universal
modulation
platform
should
be
developed,
lipid
nanovesicles
with
membrane
fusion
capabilities
(LNV‐Fs)
have
attracted
great
attention
for
this
purpose.
By
mimicking
natural
processes,
LNV‐Fs
enable
the
precise
presentation
of
immunogenic
components
on
cell
membranes,
effectively
activating
anti‐tumor
surveillance.
Similarly,
can
equip
multiple
functionalities
autologous
adoptive
effector
cells
enhanced
therapies.
Additionally,
function
vaccines
that
elicit
robust
immunity
while
promoting
long‐term
memory.
Furthermore,
different
powerful
ability
reprogramming
TME
been
reported.
Given
recent
advancements
absence
comprehensive
reviews
topic,
analysis
LNV‐F
systems,
including
their
structural
classifications,
mechanisms,
applications
cancer
provided.
future
prospects
LNV‐Fs,
particular
emphasis
artificial
intelligence‐assisted
design
are
explored.
This
review
intended
to
engage
researchers
from
diverse
interdisciplinary
fields
provide
valuable
insights
advancing
precision
immunotherapy.
