Process Biochemistry, Год журнала: 2024, Номер 145, С. 288 - 301
Опубликована: Июль 2, 2024
Язык: Английский
European Journal of Medicinal Chemistry, Год журнала: 2024, Номер 268, С. 116274 - 116274
Опубликована: Фев. 21, 2024
Язык: Английский
Процитировано
16
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 1125 - 1143
Опубликована: Фев. 1, 2024
Abstract: The rapid spread of multidrug resistance (MDR), due to abusive use antibiotics has led global health emergency, causing substantial morbidity and mortality. Bacteria attain MDR by different means such as antibiotic modification/degradation, target protection/modification/bypass, enhanced efflux mechanisms. classical approaches counteracting bacteria are expensive time-consuming, thus, it is highly significant understand the molecular mechanisms this curb problem from core level. revolutionary approach clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated sequence 9 (CRISPR/Cas9), considered a next-generation genome-editing tool presents an innovative opportunity precisely edit bacterial genome alter their strategy. Different possessing genes mec A, erm B, ram R, tet mqr B bla KPC that have been targeted CRISPR/Cas9 re-sensitize these pathogens against antibiotics, methicillin, erythromycin, tigecycline, colistin carbapenem, respectively. S. pyogenes most widely studied tool, consisting Cas9 DNA endonuclease associated with tracrRNA crRNA, which can be systematically coupled sgRNA. targeting strategies cells mediated through phage, plasmids, vesicles nanoparticles. However, specific challenging task still remains at very preliminary stage numerous obstacles awaiting solved. This review elaborates some recent updates about role system in modulating Furthermore, delivery discussed. In addition, challenges future prospects also described. Keywords: bacteria, resistance, CRISPR/Cas9, gene editing, nanoparticle,
Язык: Английский
Процитировано
14
The Science of The Total Environment, Год журнала: 2024, Номер 927, С. 172150 - 172150
Опубликована: Апрель 4, 2024
Язык: Английский
Процитировано
14
Antibiotics, Год журнала: 2024, Номер 13(9), С. 794 - 794
Опубликована: Авг. 23, 2024
The emergence of drug resistance genes and the detrimental health effects caused by overuse antibiotics are increasingly prominent problems. There is an urgent need for effective strategies to or antimicrobial in fields biomedicine therapeutics. pathogen-killing ability peptides (AMPs) linked their structure physicochemical properties, including conformation, electrical charges, hydrophilicity, hydrophobicity. AMPs a form innate immune protection found all life forms. A key aspect application involves potential combat emerging antibiotic resistance; certain against resistant microbial strains can be modified through peptide engineering. This review summarizes various used tackle resistance, with particular focus on role as agents that enhance host’s immunological functions. Most recent studies properties impregnation methods AMPs, along biomedical applications, discussed. provides researchers insights into latest advancements AMP research, highlighting compelling evidence effectiveness agents.
Язык: Английский
Процитировано
13
Advances in Colloid and Interface Science, Год журнала: 2024, Номер 325, С. 103099 - 103099
Опубликована: Фев. 1, 2024
Язык: Английский
Процитировано
12
Journal of Industrial Microbiology & Biotechnology, Год журнала: 2024, Номер 51
Опубликована: Янв. 1, 2024
The growing prevalence of fungal infections alongside rising resistance to antifungal drugs poses a significant challenge public health safety. At the close 2000s, major pharmaceutical firms began scale back on antimicrobial research due repeated setbacks and diminished economic gains, leaving only smaller companies labs pursue new solutions. Among various natural sources explored for novel compounds, peptides (AFPs) emerge as particularly promising. Despite their potential, AFPs receive less focus than antibacterial counterparts. These have been sourced extensively from nature, including plants, animals, insects, especially bacteria fungi. Furthermore, with advancements in recombinant biotechnology computational biology, can also be synthesized lab settings, facilitating peptide production. are noted wide-ranging efficacy, vitro vivo safety, ability combat biofilms. They distinguished by high specificity, minimal toxicity cells, reduced likelihood development. This review aims comprehensively cover AFPs, sources-both synthetic-their biofilm-fighting capabilities laboratory real-world action mechanisms, current status AFP research.
Язык: Английский
Процитировано
10
Microorganisms, Год журнала: 2025, Номер 13(1), С. 156 - 156
Опубликована: Янв. 14, 2025
The intensification of aquaculture has escalated disease outbreaks and overuse antibiotics, driving the global antimicrobial resistance (AMR) crisis. Antimicrobial peptides (AMPs) provide a promising alternative due to their rapid, broad-spectrum activity, low AMR risk, additional bioactivities, including immunomodulatory, anticancer, antifouling properties. AMPs derived from aquatic invertebrates, particularly marine-derived, are well-suited for aquaculture, offering enhanced stability in high-salinity environments. This study compiles analyzes data AMP databases over 200 scientific sources, identifying approximately 350 mostly cationic α-helical, across 65 protein families. While vitro assays highlight potential, limited vivo studies hinder practical application. These could serve as feed additives, therapeutic agents, or genetic engineering approaches like CRISPR/Cas9-mediated transgenesis enhance resilience farmed species. Despite challenges such stability, ecological impacts, regulatory hurdles, advancements peptidomimetics hold significant promise. Future research should emphasize refining enhancement techniques, expanding diversity bioactivity profiles, prioritizing comprehensive evaluations. Harnessing potential represents step forward on path sustainability, reducing antibiotic dependency, combating AMR, ultimately safeguarding public health ecosystem resilience.
Язык: Английский
Процитировано
2
Advanced Biology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 6, 2025
Abstract The increasing morbidity and mortality caused by multidrug‐resistant bacteria alerts human beings to the fact that conventional antibiotics are no longer reliable effective alternatives imperatively needed. Owing wide range of sources, diverse structures, unique mode action, antimicrobial peptides have been highly anticipated extensively studied in recent years. Besides, integration artificial intelligence helps researchers gain access vast unexplored chemical space, which opens more opportunities for optimization design novel structures. Moreover, Due advances chemistry synthetic biology, also begun focus on potential mimetics peptides. In this review, a comprehensive discussion about natural synthesized as well their is made, so provide summary field inspire follow‐up research.
Язык: Английский
Процитировано
2
Life Sciences, Год журнала: 2025, Номер 366-367, С. 123485 - 123485
Опубликована: Фев. 19, 2025
Язык: Английский
Процитировано
2
Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108570 - 108570
Опубликована: Март 1, 2025
This review provides a comprehensive analysis of antimicrobial peptides (AMPs), exploring their diverse sources, secondary structures, and unique characteristics. The explores into the mechanisms underlying antibacterial, immunomodulatory effects, antiviral, antiparasitic antitumour AMPs. Furthermore, it discusses three principal synthesis pathways for AMPs assesses current clinical applications preclinical research status. paper also addresses limitations AMPs, including issues related to stability, resistance, toxicity, while offering insights strategies enhancement. Recent advancements in AMP research, such as chemical modifications (including amino acid sequence optimisation, terminal side-chain modifications, PEGylation, conjugation with small molecules, photosensitisers, metal ligands, polymerisation, cyclisation specifically targeted peptides) are highlighted. goal is provide foundation future design optimisation
Язык: Английский
Процитировано
1