Characterisation of the British honey bee metagenome DOI Creative Commons
Tim Regan, Mark Barnett, Dominik R. Laetsch

и другие.

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Ноя. 20, 2018

The European honey bee (Apis mellifera) plays a major role in pollination and food production. Honey health is complex product of the environment, host genetics associated microbes (commensal, opportunistic pathogenic). Improved understanding these factors will help manage modern challenges to health. Here we used DNA sequencing characterise genomes metagenomes 19 colonies from across Britain. Low heterozygosity was observed many Scottish which had high similarity native dark bee. Colonies exhibited diversity composition relative abundance individual microbiome taxa. Most non-bee sequences were derived known commensal bacteria or pathogens. However, also detected additional fungal, protozoan metazoan species. To classify cobionts lacking genomic information, developed novel network analysis approach for clustering orphan contigs. Our analyses shed light on microbial communities with bees demonstrate power high-throughput, directed metagenomics identifying biological threats agroecosystems.

Язык: Английский

Synthetic microbiota reveal priority effects and keystone strains in the Arabidopsis phyllosphere DOI

Charlotte I. Carlström,

Christopher M. Field,

Miriam Bortfeld‐Miller

и другие.

Nature Ecology & Evolution, Год журнала: 2019, Номер 3(10), С. 1445 - 1454

Опубликована: Сен. 26, 2019

Язык: Английский

Процитировано

317
Honey bees as models for gut microbiota research DOI
Hao Zheng, Margaret I. Steele, Sean P. Leonard

и другие.

Lab Animal, Год журнала: 2018, Номер 47(11), С. 317 - 325

Опубликована: Окт. 11, 2018

Язык: Английский

Процитировано

265
Genomic diversity landscape of the honey bee gut microbiota DOI Creative Commons
Kirsten Ellegaard, Philipp Engel

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Янв. 25, 2019

Abstract The structure and distribution of genomic diversity in natural microbial communities is largely unexplored. Here, we used shotgun metagenomics to assess the honey bee gut microbiota, a community consisting few bacterial phylotypes. Our results show that most phylotypes are composed sequence-discrete populations, which co-exist individual bees age-specific abundance profiles. In contrast, strains present within these populations were found segregate into bees. Consequently, despite conserved phylotype composition, each harbors distinct at functional level. While ecological differentiation seems facilitate coexistence higher taxonomic levels, our findings suggest that, level strains, priority effects during assembly result individualized profiles, social lifestyle host. study underscores need move beyond phylotype-level characterizations understand function this community, illustrates its potential for strain-level analysis.

Язык: Английский

Процитировано

252
Gut microbiota structure differs between honeybees in winter and summer DOI Creative Commons
Lucie Kešnerová,

Olivier Emery,

Michaël Troilo

и другие.

The ISME Journal, Год журнала: 2019, Номер 14(3), С. 801 - 814

Опубликована: Дек. 13, 2019

Abstract Adult honeybees harbor a specialized gut microbiota of relatively low complexity. While seasonal differences in community composition have been reported, previous studies focused on compositional changes rather than absolute bacterial loads. Moreover, little is known about the winter bees, which live much longer bees during foraging season, and are critical for colony survival. We quantified seven core members bee single over 2 years characterized 14 colonies summer winter. Our data show that total loads substantially differ between foragers, nurses, bees. Long-lived had highest lowest α-diversity, with characteristic shift toward high levels Bartonella Commensalibacter, reduction opportunistic colonizers. Using gnotobiotic experiments, we diet major contributor to observed Overall, our study reveals remarkably different from foragers nurses. Considering importance survival, future work should focus role health disease.

Язык: Английский

Процитировано

250
Mechanisms underlying gut microbiota–host interactions in insects DOI Open Access

Konstantin Schmidt,

Philipp Engel

Journal of Experimental Biology, Год журнала: 2021, Номер 224(2)

Опубликована: Янв. 15, 2021

ABSTRACT Insects are the most diverse group of animals and colonize almost all environments on our planet. This diversity is reflected in structure function microbial communities inhabiting insect digestive system. As mammals, gut microbiota insects can have important symbiotic functions, complementing host nutrition, facilitating dietary breakdown or providing protection against pathogens. There an increasing number models that experimentally tractable, mechanistic studies microbiota–host interactions. In this Review, we will summarize recent findings advanced understanding molecular mechanisms underlying symbiosis between their microbiota. We open article with a general introduction to then turn towards discussion particular processes governing colonization environment as well beneficial roles mediated by The Review highlights that, although active field research implications for fundamental applied science, still early stage mechanisms. However, expanding capability culture microbiomes manipulate microbe–host interactions promises new insights from symbioses.

Язык: Английский

Процитировано

106
Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi DOI Creative Commons
Andrew Quinn, Yassine El Chazli, Stéphane Escrig

и другие.

Nature Microbiology, Год журнала: 2024, Номер 9(2), С. 477 - 489

Опубликована: Янв. 15, 2024

Diverse bacteria can colonize the animal gut using dietary nutrients or by engaging in microbial crossfeeding interactions. Less is known about role of host-derived enabling bacterial colonization. Here we examined metabolic interactions within evolutionary ancient symbiosis between honey bee (Apis mellifera) and core microbiota member Snodgrassella alvi. This betaproteobacterium incapable metabolizing saccharides, yet colonizes presence a sugar-only diet. Using comparative metabolomics,

Язык: Английский

Процитировано

22
The interaction and response of gut microbes to exposure to chiral ethiprole in honeybees (Apis mellifera) DOI
Yonghong Zhang, Jianhui Liu,

Jingliang Shi

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер 486, С. 137112 - 137112

Опубликована: Янв. 5, 2025

Язык: Английский

Процитировано

3
An Ecological Loop: Host Microbiomes across Multitrophic Interactions DOI
Hongwei Liu, Catriona A. Macdonald, James M. Cook

и другие.

Trends in Ecology & Evolution, Год журнала: 2019, Номер 34(12), С. 1118 - 1130

Опубликована: Авг. 15, 2019

Язык: Английский

Процитировано

124
Missing Microbes in Bees: How Systematic Depletion of Key Symbionts Erodes Immunity DOI
Brendan A. Daisley,

John A. Chmiel,

Andrew P. Pitek

и другие.

Trends in Microbiology, Год журнала: 2020, Номер 28(12), С. 1010 - 1021

Опубликована: Июль 14, 2020

Язык: Английский

Процитировано

100
The queen’s gut refines with age: longevity phenotypes in a social insect model DOI Creative Commons
Kirk E. Anderson, Vincent Ricigliano,

Brendon M. Mott

и другие.

Microbiome, Год журнала: 2018, Номер 6(1)

Опубликована: Июнь 18, 2018

In social insects, identical genotypes can show extreme lifespan variation providing a unique perspective on age-associated microbial succession. honey bees, short- and long-lived host phenotypes are polarized by suite of factors including hormones, nutrition, immune senescence, oxidative stress. Similar to other model organisms, the aging gut microbiota short-lived (worker) bees accrue Proteobacteria depleted Lactobacillus Bifidobacterium, consistent with senescence markers. contrast, (queen) maintain youthful cellular function much lower expression stress genes, suggesting very different environment for succession.We sequenced 63 bee queens exploring two chronological ages four alimentary tract niches. To control genetic environmental variation, we quantified carbonyl accumulation in queen fat body tissue as proxy biological aging. We compared our results age-specific succession worker guts. Accounting source or more bacterial species per niche differed significantly age. Biological was correlated composition highlighting relationship Queens workers shared many major species, but differ markedly community structure age stark contrast workers, associated increased Bifidobacterium depletion various Proteobacteria.We present system linking changes diet longevity, most confounding variables human research. The pattern is largely reverse that demonstrated workers. guts progressively dominated three Proteobacteria, these same were sparse phenotypes. More broadly, age-related reflect regulatory anatomy reproductive metabolism. Our synthesis suggests evolution colony-level physiology formed context host-microbial interactions microbiota.

Язык: Английский

Процитировано

93