Division of labor in honey bee gut microbiota for plant polysaccharide digestion

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(51), С. 25909 - 25916

Опубликована: Ноя. 27, 2019

Bees acquire carbohydrates from nectar and lipids; amino acids pollen, which also contains polysaccharides including cellulose, hemicellulose, pectin. These potential energy sources could be degraded fermented through microbial enzymatic activity, resulting in short chain fatty available to hosts. However, the contributions of individual microbiota members polysaccharide digestion have remained unclear. Through analysis bacterial isolate genomes a metagenome honey bee gut microbiota, we identify that Bifidobacterium Gilliamella are principal degraders hemicellulose Both show extensive strain-level diversity gene repertoires linked digestion. Strains bees possess more such genes than strains bumble bees. In Bifidobacterium, encoding carbohydrate-active enzymes colocated within loci devoted utilization, as Bacteroides human gut. Carbohydrate-active enzyme-encoding expressions up-regulated response particular hemicelluloses both vitro vivo. Metabolomic analyses document experimentally colonized by different generate distinctive metabolomic profiles, with enrichment for specific monosaccharides, corresponding predictions genomic data. The other 3 core species clusters (Snodgrassella 2 Lactobacillus clusters) few or no Together, these findings indicate strain composition hosts determines metabolic capabilities potentially affects host nutrition. Furthermore, niche specialization revealed our study may promote overall community stability microbiomes

Язык: Английский

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Simple animal models for microbiome research

Nature Reviews Microbiology, Год журнала: 2019, Номер 17(12), С. 764 - 775

Опубликована: Авг. 15, 2019

Язык: Английский

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Honeybee gut Lactobacillus modulates host learning and memory behaviors via regulating tryptophan metabolism

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Апрель 19, 2022

Honeybees are highly social insects with a rich behavioral repertoire and versatile model for neurobiological research. Their gut microbiota comprises limited number of host-restricted bacterial phylotypes that important honeybee health. However, it remains unclear how specific members affect behaviors. Here, we find antibiotic exposure disturbs the community influences phenotypes under field conditions. Using laboratory-generated gnotobiotic bees, show normal is required olfactory learning memory abilities. Brain transcriptomic profiling reveals distinct brain gene expression patterns between microbiota-free conventional bees. Subsequent metabolomic analyses both hemolymph samples mainly regulates tryptophan metabolism. Our results indicate host-specific Lactobacillus strains promote behavior by transforming to indole derivatives activate host aryl hydrocarbon receptor. findings highlight contributions neurological processes, thus providing promising understand host-microbe interactions.

Язык: Английский

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Specific Strains of Honeybee Gut Lactobacillus Stimulate Host Immune System to Protect against Pathogenic Hafnia alvei

Microbiology Spectrum, Год журнала: 2022, Номер 10(1)

Опубликована: Янв. 5, 2022

Honeybee gut microbiota plays an important role in host physiology and metabolism. Recent studies have shown that the influence of resident microorganisms regulation honeybee immune system is profound, which protects against pathogen Serratia marcescens. However, only few core members functions been studied. Here, we explored how different bee bacterial species aided clearance pathogenic Hafnia alvei, causes septicemia with a high mortality rate. We found both Gilliamella apicola W8136 Lactobacillus apis W8172 protect honeybees from opportunistic pathogen, while two other strains did not affect invasion H. alvei. Transcriptomic analysis revealed induced expression profiles gut. Specifically, regulator genes Toll pathway, PGRP-S3 recognizing Gram-positive Spätzle bind to protein for downstream signal transduction, were elevated by L. apis. Correspondingly, multiple encoding antibacterial proteins also stimulated Interestingly, increased apidaecin, exhibited vitro inhibitory effect on To elucidate difference host's regulation, comparative genomic analyses indicate S-layer unique are potentially involved signaling activation production. IMPORTANCE Honeybees essential pollinators supporting global agricultural economies food supplies. decline has linked several factors, infection considered one most significant contributing factors. Although limited number pathogens identified, alvei causing adult bees. In this study, showed members, Lactobacillus, can clear invasion. Mono-colonization specific stimulate pathway AMPs. apidaecin upregulated symbionts more effective pathogen. Moreover, our suggests surface-layer driver signaling, highlighting variation regulating system.

Язык: Английский

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Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi

Nature Microbiology, Год журнала: 2024, Номер 9(2), С. 477 - 489

Опубликована: Янв. 15, 2024

Diverse bacteria can colonize the animal gut using dietary nutrients or by engaging in microbial crossfeeding interactions. Less is known about role of host-derived enabling bacterial colonization. Here we examined metabolic interactions within evolutionary ancient symbiosis between honey bee (Apis mellifera) and core microbiota member Snodgrassella alvi. This betaproteobacterium incapable metabolizing saccharides, yet colonizes presence a sugar-only diet. Using comparative metabolomics,

Язык: Английский

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Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions

Biomolecules, Год журнала: 2020, Номер 10(6), С. 923 - 923

Опубликована: Июнь 18, 2020

Stingless bees are a type of honey producers that commonly live in tropical countries. Their use for is being abandoned due to its limited production. However, the recent improvements stingless bee production, particularly South East Asia, have brought products back into picture. Although there many species produce wide spread products, known since old eras traditional medicine, modern medical community still missing more investigational studies on products. Whereas comprehensive current era attest biological and medicinal properties honeybee (Apis mellifera) less known. This review highlights first time benefits (honey, propolis, pollen cerumen), investigations promising future directions. emphasizes potential antioxidant these turn play vital role preventing treating diseases associated with oxidative stress, microbial infections inflammatory disorders. Summarizing all data insights one manuscript may increase commercial value as food ingredient. will also highlight utility context therapeutic properties, some which yet be discovered.

Язык: Английский

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Oral or Topical Exposure to Glyphosate in Herbicide Formulation Impacts the Gut Microbiota and Survival Rates of Honey Bees

Applied and Environmental Microbiology, Год журнала: 2020, Номер 86(18)

Опубликована: Июль 10, 2020

The honey bee gut microbial community plays a vital role in immune response and defense against opportunistic pathogens. Environmental stressors, such as the herbicide glyphosate, may affect microbiota, with negative consequences for health. Glyphosate is usually sprayed field mixed adjuvants, which enhance herbicidal activity. These adjuvants also undesired effects nontargeted organisms. This seems to be case glyphosate-based on bees. As we show this study, oral exposure either pure glyphosate or commercial formulation perturbs microbiota of bees, topical has direct effect health, increasing mortality dose-dependent way leaving surviving bees perturbed microbiota. Understanding formulations help protect these important agricultural pollinators.

Язык: Английский

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Advances in Microbiome Research for Animal Health

Annual Review of Animal Biosciences, Год журнала: 2020, Номер 9(1), С. 289 - 311

Опубликована: Дек. 15, 2020

Host-associated microbiomes contribute in many ways to the homeostasis of metaorganism. The microbiome's contributions range from helping provide nutrition and aiding growth, development, behavior protecting against pathogens toxic compounds. Here we summarize current knowledge diversity importance microbiome animals, using representative examples wild domesticated species. We demonstrate how beneficial ecological roles animal-associated can be generally grouped into well-defined main categories microbe-based alternative treatments applied mitigate problems for both economic conservation purposes crucial about host-microbiota symbiotic interactions. suggest a Customized Combination Microbial-Based Therapies promote animal health practice sustainable husbandry. also discuss connections threats associated with biodiversity loss, microorganism extinction, emerging diseases, such as COVID-19 pandemic.

Язык: Английский

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Symbionts shape host innate immunity in honeybees

Proceedings of the Royal Society B Biological Sciences, Год журнала: 2020, Номер 287(1933)

Опубликована: Авг. 26, 2020

The gut microbiome plays a critical role in the health of many animals. Honeybees are no exception, as they host core that affects their nutrition and immune function. However, relationship between honeybee system its symbionts is poorly understood. Here, we explore how beneficial symbiont Snodgrassella alvi gene expression. We show both live heat-killed S. protect honeybees from opportunistic pathogen Serratia marcescens lead to expression antimicrobial peptides . Honeybee genes respond differently compared alvi, latter causing more extensive response. preference for Toll pathway upregulation over Imd presence Finally, find aids clearance gut, supporting potential colonization resistance. Our results by triggers replicable These responses may benefit symbiont, helping regulate microbial members preventing overgrowth or invasion opportunists.

Язык: Английский

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Microbiome-mediated plasticity directs host evolution along several distinct time scales

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2020, Номер 375(1808), С. 20190589 - 20190589

Опубликована: Авг. 9, 2020

Host-associated microbiomes influence their host's fitness in myriad ways and can be viewed as a source of phenotypic plasticity. This plasticity may allow the host to accommodate novel environmental challenges thus evolutionary adaptation. As with other modalities phenomena such Baldwin effect genetic assimilation, microbiome-mediated adaptation by facilitating accelerating it, slowing it down, or even preventing it. The dynamics involved are likely more complex than those previously studied related plasticity, involve different processes on each time scale, acquired recognition newly associated microbes immune system single- multiple-generation scales, selection transmission between hosts, acting longer scales. To date, is unclear if how any these shape evolution. opinion piece article provides conceptual framework for considering which directs evolution concludes suggestions key experimental tests presented ideas. part theme issue 'The role microbiome evolution'.

Язык: Английский

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