This
short
communication
discusses
the
duplication
issue
in
Scopus's
new
interface.
After
searching
for
bibliographic
records,
author
noticed
many
duplicates
downloadable
file.
lasted
a
month,
with
67%
of
records
duplicated.
alerting
Scopus,
version
was
confirmed
to
have
issue,
prompting
recommendation
revert
back
old
version.
The
study
advises
caution
when
utilizing
Scopus
data,
particularly
exporting
large
files.
Additionally,
it
highlights
novel
attributes
interface,
including
ability
download
20,000
at
time,
notable
increase
from
previous
limit
2,000.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 23, 2024
An
increasing
population,
climate
change,
and
diminishing
natural
resources
present
severe
threats
to
global
food
security,
with
traditional
breeding
genetic
engineering
methods
often
falling
short
in
addressing
these
rapidly
evolving
challenges.
CRISPR/Cas
systems
have
emerged
as
revolutionary
tools
for
precise
modifications
crops,
offering
significant
advancements
resilience,
yield,
nutritional
value,
particularly
staple
crops
like
rice
maize.
This
review
highlights
the
transformative
potential
of
technology,
emphasizing
recent
innovations
such
prime
base
editing,
development
novel
CRISPR-associated
proteins,
which
significantly
improved
specificity,
efficiency,
scope
genome
editing
agriculture.
These
enable
targeted
that
enhance
tolerance
abiotic
stresses
well
biotic
stresses.
Additionally,
plays
a
crucial
role
improving
crop
yield
quality
by
enhancing
photosynthetic
nutrient
uptake,
resistance
lodging,
while
also
taste,
texture,
shelf
life,
content
through
biofortification.
Despite
challenges
off-target
effects,
need
more
efficient
delivery
methods,
ethical
regulatory
concerns,
underscores
importance
security
sustainability
It
calls
continued
research
integration
CRISPR
other
emerging
technologies
nanotechnology,
synthetic
biology,
machine
learning
fully
realize
its
developing
resilient,
productive,
sustainable
agricultural
systems.
Microsystems & Nanoengineering,
Год журнала:
2025,
Номер
11(1)
Опубликована: Фев. 20, 2025
The
microinjection
of
Zebrafish
embryos
is
significant
to
life
science
and
biomedical
research.
In
this
article,
a
novel
automated
system
developed
for
cell
microinjection.
A
sophisticated
microfluidic
chip
designed
transport,
hold,
inject
cells
continuously.
For
the
first
time,
microinjector
with
microforce
perception
proposed
integrated
within
enclosed
judge
whether
have
been
successfully
punctured.
deep
learning
model
employed
detect
yolk
center
zebrafish
locate
position
injection
needle
yolk,
which
enables
enhancing
precision
injection.
prototype
fabricated
achieve
automatic
batch
Experimental
results
demonstrated
that
efficiency
about
20
seconds
per
cell.
Cell
puncture
success
rate
survival
are
100%
84%,
respectively.
Compared
manual
operation,
improves
operation
rate.
has
potential
greatly
reduce
workload
experimenters
shorten
relevant
study
period.
FEBS Letters,
Год журнала:
2023,
Номер
597(13), С. 1679 - 1680
Опубликована: Июнь 19, 2023
Photosynthesis
uses
the
energy
of
sunlight
to
convert
water
and
atmospheric
CO2
into
sugars,
providing
food
oxygen
for
life.
The
fixation
in
this
crucial
biological
process
is
mediated
by
enzyme
Rubisco.
inefficiencies
Rubisco
have
inspired
researchers
decades
explore
ways
improve
its
function
with
goal
increasing
crop
yields
[1–4],
more
recently
combat
global
warming
[5].
In
graphical
review
we
highlight
challenges
involved
engineering
plant
Rubisco,
a
focus
on
extensive
chaperone
requirement
biogenesis.
We
discuss
strategies
catalytic
properties
sequestering
membraneless
compartments
increase
fixation.
iScience,
Год журнала:
2024,
Номер
27(3), С. 109053 - 109053
Опубликована: Фев. 1, 2024
The
optimization
of
the
CRISPR-Cas9
system
for
enhancing
editing
efficiency
holds
significant
value
in
scientific
research.
In
this
study,
we
optimized
single
guide
RNA
and
The Plant Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 8, 2024
SUMMARY
The
implementation
of
genome
editing
strategies
in
grapevine
is
the
easiest
way
to
improve
sustainability
and
resilience
while
preserving
original
genotype.
Among
others,
Mildew
Locus‐O
(
MLO
)
genes
have
already
been
reported
as
good
candidates
develop
powdery
mildew‐immune
plants.
A
never‐explored
target
NPR3
,
a
negative
regulator
systemic
acquired
resistance.
We
report
exploitation
cisgenic
approach
with
Cre‐lox
recombinase
technology
generate
grapevine‐edited
plants
potential
be
transgene‐free
their
genetic
background.
characterization
three
edited
lines
for
each
demonstrated
immunity
development
against
Erysiphe
necator
MLO6‐7
‐edited
Concomitantly,
significant
improvement
resilience,
associated
increased
leaf
thickness
specific
biochemical
responses,
was
observed
defective
E.
Plasmopara
viticola
.
Transcriptomic
analysis
revealed
that
both
modulated
gene
expression
profiles,
pointing
distinct
though
partially
overlapping
responses.
Furthermore,
targeted
metabolite
highlighted
an
overaccumulation
stilbenes
coupled
improved
oxidative
scavenging
targets,
likely
protecting
mutants
from
detrimental
pleiotropic
effects.
Finally,
Cre‐loxP
allowed
recovery
one
plant
complete
removal
transgene.
Taken
together,
our
achievements
provide
comprehensive
understanding
molecular
adjustments
occurring
double
‐defective
grape
In
parallel,
multiple
purposes
has
demonstrated,
raising
new
questions
on
its
wide
role
orchestrating
biotic
stress
Journal of Genetic Engineering and Biotechnology,
Год журнала:
2024,
Номер
22(2), С. 100380 - 100380
Опубликована: Апрель 30, 2024
An
effective
CRISPR/Cas9
reagent
delivery
system
has
been
developed
in
a
commercially
significant
crop,
the
chilli
pepper
using
construct
harboring
two
distinct
gRNAs
targeting
exons
14
and
15
of
Phytoene
desaturase
(CaPDS)
gene,
whose
loss-of-function
mutation
causes
photo-bleaching
phenotype
impairs
biosynthesis
carotenoids.
The
carrying
sgRNAs
was
observed
to
create
visible
albino
phenotypes
cotyledons
regenerating
on
medium
containing
80
mg/L
kanamycin,
plants
regenerated
therefrom
after
biolistic-mediated
transfer
reagents
into
cells.
Analysis
genome-editing
events,
including
kanamycin
screening
mutants
assessing
homozygosity
T7
endonuclease
assay
(T7E1),
revealed
62.5
%
transformed
exhibited
successful
editing
at
target
region
displayed
both
mosaic
phenotypes.
Interestingly,
sequence
analysis
showed
that
insertions
substitutions
were
present
all
plant
lines
targeted
CaPDS
region.
detected
mutations
mostly
12-
24-bp
deletions
disrupted
exon-intron
junction,
along
with
base
insertion
1-bp
protospacer
adjacent
motif
(PAM)
site.
reduction
essential
photosynthetic
pigments
(chlorophyll
a,
chlorophyll
b
carotenoid)
knockout
provided
further
evidence
gene
had
functionally
disrupted.
In
this
study,
we
report
biolistic
peppers
is
very
produces
multiple
events
short
span
time.
