AMP-Mimetic Antimicrobial Polymer-Involved Synergic Therapy with Various Coagents for Improved Efficiency

Biomacromolecules, Год журнала: 2024, Номер 25(8), С. 4619 - 4638

Опубликована: Май 8, 2024

The misuse of antibiotics contributes to the emergence multidrug-resistant (MDR) bacteria. Infections caused by MDR bacteria are rapidly evolving into a significant threat global healthcare due lack effective and safe treatments. Antimicrobial peptides (AMPs) with broad-spectrum antibacterial activity kill generally through membrane disruption mechanism; hence, they tend not induce resistance readily. However, AMPs exhibit disadvantages, such as high cost susceptibility proteolytic degradation, which limit their clinical application. AMP-mimetic antimicrobial polymers, low cost, stability proteolysis, activity, negligible resistance, rapid bactericidal effect, have received extensive attention new type drugs. Lately, polymer-involved synergic therapy provides superior alternative combat distinct mechanisms. In this Review, we summarize polymers involved in therapy, particularly focusing on different combinations between commercially available antimicrobials, organic small molecule photosensitizers, inorganic nanomaterials, nitric oxide.

Язык: Английский

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Self-Assembled Borneol-Guanidine-Based Amphiphilic Polymers as an Efficient Antibiofilm Agent

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(29), С. 38429 - 38441

Опубликована: Июнь 29, 2024

Biofilm-associated infections remain a tremendous obstacle to the treatment of microbial globally. However, poor penetrability dense extracellular polymeric substance matrix traditional antibacterial agents limits their antibiofilm activity. Here, we show that nanoaggregates formed by self-assembly amphiphilic borneol-guanidine-based cationic polymers (BGNx-n) possess strong activity and can eliminate mature Staphylococcus aureus (S. aureus) biofilms. The introduction guanidine moiety improves hydrophilicity membrane BGNx-n. self-assembled with highly localized positive charges are expected enhance interaction negatively charged bacteria Furthermore, dissociate on surface biofilms into smaller BGNx-n polymers, which enhances ability penetrate exhibit superior have minimum inhibitory concentration (MIC) 62.5 μg·mL–1 against S. eradicate at 4 × MIC negligible hemolysis. Taken together, this size-variable system offers promising strategy for development effective agents.

Язык: Английский

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Interface/Dipole Polarized Antibiotics‐Loaded Fe₃O₄/PB Nanoparticles for Non‐Invasive Therapy of Osteomyelitis Under Medical Microwave Irradiation

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Сен. 30, 2024

Abstract Due to their poor light penetration, photothermal therapy and photodynamic are ineffective in treating deep tissue infections, such as osteomyelitis caused by Staphylococcus aureus ( S. ). Here, a microwave (MW)‐responsive magnetic targeting composite system consisting of ferric oxide (Fe 3 O 4 )/Prussian blue (PB) nanoparticles, gentamicin (Gent), biodegradable poly(lactic‐co‐glycolic acid) (PLGA) is reported. The PLGA/Fe /PB/Gent complex used combination with MW thermal (MTT), dynamic (MDT), chemotherapy (CT) treat acute infected ‐infected. powerful antibacterial effect the determined synergistic effects heat reactive oxygen species (ROS) generation Fe /PB nanoparticles under irradiation effective release Gent at infection site via targeting. mechanism analyzed using bacterial transcriptome RNA sequencing. ROS reduce activity protein transporters on membrane, along transport various ions acceleration phosphate metabolism, which can lead increased permeability damage ribosome DNA, accompany internal efflux bacteria, thus effectively killing bacteria.

Язык: Английский

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Photothermal‐Mediated In Situ Delivery of Polycations Into Bacteria via Alternating Polymer‐Modified Nanoparticles for Targeted Bacterial Killing and Enhanced Biofilm Penetration

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 18, 2024

Abstract To achieve targeted clearance of bacteria and their biofilms, a straightforward strategy to integrate antimicrobial alternating polymers with photothermal polydopamine nanoparticles (PDA NPs) is proposed. By manipulating the distribution electrostatic, hydrophobic, hydrogen bonding units in polymer backbone, thermal stability polymer‐particle interaction can be tuned, enabling photothermal‐mediated situ delivery cationic into bacteria. The coating significantly enhances penetration capability NPs Methicillin‐resistant Staphylococcus aureus ( MRSA ) Escherichia coli E. biofilm, achieving 99% bactericidal rate within biofilm at low concentration after 10 min near‐infrared irradiation, whereas pristine PDA shows negligible effects. in‐vitro co‐culture model demonstrates that alternating‐polymer‐loaded selectively eradicate 1 × 7 CFU mL −1 while preserving over 75% viability mammalian cells, including mice fibroblasts, human kidney cervical cancer macrophages. efficacy these biocompatible targeting further validated mouse ‐infected wound model. This approach represents significant advancement developing safe efficient therapies bacterial killing minimal off‐target effects based on polymers.

Язык: Английский

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Structural Element of Vitamin U‐Mimicking Antibacterial Polypeptide with Ultrahigh Selectivity for Effectively Treating MRSA Infections

Angewandte Chemie International Edition, Год журнала: 2023, Номер 63(7)

Опубликована: Дек. 22, 2023

Antimicrobial peptides (AMPs) exhibit mighty antibacterial properties without inducing drug resistance. Achieving much higher selectivity of AMPs towards bacteria and normal cells has always been a continuous goal to be pursued. Herein, series sulfonium-based polypeptides with different degrees branching polymerization were synthesized by mimicking the structure vitamin U. The polypeptide, G

Язык: Английский

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Emerging Trends in Dissolving-Microneedle Technology for Antimicrobial Skin-Infection Therapies

Pharmaceutics, Год журнала: 2024, Номер 16(9), С. 1188 - 1188

Опубликована: Сен. 8, 2024

Skin and soft-tissue infections require significant consideration because of their prolonged treatment duration propensity to rapidly progress, resulting in severe complications. The primary challenge stems from the involvement drug-resistant microorganisms that can form impermeable biofilms, as well possibility infection extending deep into tissues, thereby complicating drug delivery. Dissolving microneedle patches are an innovative transdermal drug-delivery system effectively enhances penetration through stratum corneum barrier, increasing concentration at site infection. They offer highly efficient, safe, patient-friendly alternatives conventional topical formulations. This comprehensive review focuses on recent advances emerging trends dissolving-microneedle technology for antimicrobial skin-infection therapy. Conventional antibiotic microneedles compared with those based agents, such quorum-sensing inhibitors, peptides, antimicrobial-matrix materials. also highlights potential incorporating chemodynamic, nanoenzyme antimicrobial, photodynamic, photothermal antibacterial therapies. explores advantages various therapies emphasizes combined application improve efficacy microneedles. Finally, this analyzes druggability different discusses possible future developments.

Язык: Английский

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Open-vessel polymerization of N-carboxyanhydride (NCA) for polypeptide synthesis

Nature Protocols, Год журнала: 2024, Номер unknown

Опубликована: Окт. 8, 2024

Язык: Английский

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Shape matters: Effect of amphiphilic polymer topology on antibacterial activity and hemocompatibility

European Polymer Journal, Год журнала: 2023, Номер 205, С. 112698 - 112698

Опубликована: Дек. 20, 2023

Understanding the intricate relationship between polymer architecture and biological activity is critical for advancing development of antibacterial polymers. In this study, we systematically investigated properties hemocompatibility 27 different synthetic polymers prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization. The activities these were probed against two gram-negative bacteria, namely Pseudomonas aeruginosa PAO1 Escherichia coli K12. Furthermore, efficacy correlated with their topology, encompassing linear (LPs), hyperbranched (HPs), star (SPs), characterized varying hydrophobic compositions (20 %, 25 30 %). HPs using types crosslinking monomer (acrylate vs. acrylamide). [crosslinker]:[RAFT agent] ratio in synthesis greatly affect hemocompatibility, while type crosslinker had a negligible impact. showed remarkable 2- to 4-fold enhancement compared LPs at same ratio. Interestingly, SPs low ratio, did not display toxicity towards red blood cells, maintaining similar potent as HPs. Overall, exhibited superior bioactivity (SPs > LPs), demonstrating that manipulation topology offers promising avenue enhancing performance

Язык: Английский

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A dual-enhancement antibacterial strategy for hierarchically functionalized surfaces with photodynamic and antifouling performance

Materials Today Chemistry, Год журнала: 2024, Номер 36, С. 101945 - 101945

Опубликована: Фев. 1, 2024

Язык: Английский

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Peptide-mimicking poly(2-oxazoline) displaying potent antibacterial and antibiofilm activities against multidrug-resistant Gram-positive pathogenic bacteria

Journal of Material Science and Technology, Год журнала: 2024, Номер 214, С. 233 - 244

Опубликована: Июль 16, 2024

Язык: Английский

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