Cellular Immune Responses in Lymphoid Tissues of Broiler Chickens Experimentally Infected with Necrotic Enteritis–Producing Clostridium perfringens Strains

Avian Diseases, Год журнала: 2023, Номер 67(2)

Опубликована: Июнь 29, 2023

Respuestas inmunes celulares en tejidos linfoides de pollos engorde infectados experimentalmente con cepas Clostridium perfringens productoras enteritis necrótica. Las respuestas del huésped contra (CP), el agente causante la necrótica (NE) pollos, son poco conocidas. En presente estudio, primero se analizó capacidad producción siete C. netB+ (CP5, CP18, CP26, CP64, CP67, CP68 y NCNE-1), utilizando un modelo infección experimental engorde. La evaluación las lesiones macroscópicas intestinales mostró que todas cepas, excepto CP5, podían producir necrótica, mientras CP26 CP64 produjeron relativamente más severas comparación los otros grupos. Posteriormente, evaluaron tonsilas cecales (CT), bolsa Fabricio bazo representan variaciones nivel virulencia, por ejemplo CP5 avirulenta, CP18 virulenta cepa virulenta. El análisis inmunofenotipado frecuencias macrófagos esplénicos o fueron significativamente altas controles no infectados, células T γd B grupos CP18. tenían número mayor esplénicas CD4+ CD8+ expresaban moléculas activación CD44 CD28, también una frecuencia estas activadas CP5. conjunto, estos hallazgos sugirieron celulares, incluida T, inducen selectivamente virulentas características este patógeno pueden influir resultado inmunidad

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Comparative Efficacy of Plant Extracts and Probiotics on Growth and Gut Health in Chickens with Necrotic Enteritis

Animals, Год журнала: 2024, Номер 14(22), С. 3312 - 3312

Опубликована: Ноя. 18, 2024

Necrotic enteritis (NE), caused by

Язык: Английский

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Dextran sulfate-coated curcumin nanocrystals for the treatment of DSS-induced ulcerative colitis in mice

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125428 - 125428

Опубликована: Март 1, 2025

Язык: Английский

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Phloretin supplementation ameliorates intestinal injury of broilers with necrotic enteritis by alleviating inflammation, enhancing antioxidant capacity, regulating intestinal microbiota, and producing plant secondary metabolites

Poultry Science, Год журнала: 2025, Номер unknown, С. 105187 - 105187

Опубликована: Апрель 1, 2025

The present study aimed to explore the effects of dietary phloretin (PT) on growth performance, immune response, and intestinal function in broilers with necrotic enteritis (NE). A total 288 1-day-old Arbor Acres chicks were assigned 3 groups, 8 replicates per group 12 replicate. Over 6 weeks, birds fed a basal diet or same supplemented 200 mg/kg phloretin. Birds challenged groups inoculated coccildia during d 7 9 Clostridium perfringens(CP) 14 18. Results showed that CP coccidia challenge reduced average daily gain feed intake, increased conversion ratio broilers, induced inflammation oxidative stress, inhibited mRNA expression levels for genes associated barrier nutrient transporters (P < 0.05). PT addition improved performance at early phase morphology, elevated antioxidant capacity via increasing activity superoxide dismutase ileum 0.01). Dietary regulated intesetinal as observed by increases content secretory IgA decreased cytokines (Interleukin-1β, Interleukin-10) Moreover, NE infection significantly disrupted balance flora, led lower level short-chain fatty acids such butyric acid concentration ileum, while microbiota structure, acetic 0.001). Furthermore, metabolomics analysis indicated treatment improve plant secondary metabolites contents like 2'-o-glucuronide. Additionally, we significant positive correlation among PT, Ligilactobacillus acid, between metabolites. Overall, supplementation could ameliorate injury enhancing function, regulating flora structure producing

Язык: Английский

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In Silico Design, Optimization, and Evaluation of a Multi-Epitope Vaccine Targeting the Clostridium perfringens Collagen Adhesin Protein

Microorganisms, Год журнала: 2025, Номер 13(5), С. 1147 - 1147

Опубликована: Май 16, 2025

Necrotic enteritis (NE), caused by pathogenic Clostridium perfringens, poses a significant threat to global poultry health, with estimated annual losses exceeding USD 6 billion. The rising incidence of NE has been associated the reduced use antibiotic growth promoters, underscoring urgent need for alternative control measures such as vaccination. Collagen adhesin protein (CNA), key virulence factor in pathogenesis, represents promising vaccine target. US Food and Drug Administration begun phasing out animal testing requirements biologics monoclonal antibody drugs. In this study, computational multi-epitope (MEV) targeting CNA was designed integrating predicted Cluster Differentiation (CD)4+ helper T lymphocyte (Th), CD8+ cytotoxic (CTL), B-cell epitopes. Bioinformatics tools were used identify immunogenic, antigenic, non-allergenic epitopes assembled into 115-amino-acid peptide construct. candidate demonstrated strong stability solubility. silico immune simulation robust responses, including elevated IgG IgM levels, plasma cell proliferation, Th memory formation, CTL activation, comparable responses elicited full-length CNA. These findings support potential one multiple effective components, offering antibiotic-based approaches disease management.

Язык: Английский

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Clostridium butyricum: a promising approach to enhancing intestinal health in poultry

Frontiers in Veterinary Science, Год журнала: 2025, Номер 12

Опубликована: Май 21, 2025

The intestinal tract is essential for the overall health and productivity of animals, including poultry. Clostridium butyricum (C. butyricum) a probiotic bacterium that has been shown to be promising candidate improving function subsequently optimizing poultry growth. beneficial effects C. on can attributed several key mechanisms. First, it helps maintain balance microbiota by inhibiting growth harmful bacteria promoting proliferation bacteria. This microbial homeostasis efficient nutrient digestion absorption. Second, enhances integrity barrier. It epithelial tight junctions, reducing permeability mucosa preventing invasion pathogenic substances. Furthermore, participates in regulation immune responses within environment. stimulates production immunoglobulins cytokines, enhancing defense mechanisms host. Additionally, influences metabolism nutrients intestine. promotes synthesis short-chain fatty acids (SCFAs), which provide an energy source cells contribute maintaining healthy Intestinal basis animal growth, ultimately performance regulating health. Studies have demonstrated administration leads improved feed conversion efficiency, increased weight gain, enhanced However, further research still needed fully elucidate complex interactions precise molecular underlying its effects. Understanding these detail will not only important insights efficiency but also development more effective sustainable strategies industry.

Язык: Английский

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Dietary Lactobacillus fermentum and Lactobacillus paracasei improve the intestinal health of broilers challenged with coccidia and Clostridium perfringens

Frontiers in Veterinary Science, Год журнала: 2022, Номер 9

Опубликована: Дек. 15, 2022

Necrotic enteritis (NE) is a great threat to the intestinal health of broilers, resulting in decreased growth performance and significant economic losses. Lactobacillus fermentum (LF) paracasei (LP) exert beneficial effects on health. The aim present study was investigate dietary LF LP broilers challenged with coccidia Clostridium perfringens (CCP). animal trial carried out using 336 (Ross 308) for 35 days completely randomized design. were divided into 4 groups based treatment as follows: control (CTR) group fed basal diet without CCP challenge challenge. CCP+LF CCP+LP by CCP, meanwhile, (1 × 109 CFU/g) supplemented diets, respectively. results showed that morphology negatively affected In addition, number digesta relative abundance Escherichia coli cecal increased. Besides, mRNA level IgA jejunum downregulated, transcript IL-8 upregulated Dietary failed improve However, they barrier function. able alleviate downregulation TGF-β spleen lesion scores compared group. Furthermore, alleviated upregulation reduced ileal digesta. conclusion, mitigate negative infection performance; however, strengthening alleviating inflammation.

Язык: Английский

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Association of Bacillus subtilis and Bacillus amyloliquefaciens: minimizes the adverse effects of necrotic enteritis in the gastrointestinal tract and improves zootechnical performance in broiler chickens

Poultry Science, Год журнала: 2023, Номер 103(3), С. 103394 - 103394

Опубликована: Дек. 19, 2023

This study aimed to evaluate the efficiency and capacity of probiotic composed Bacillus subtilis amyloliquefaciens, in improving zootechnical performance broiler chickens challenged with Eimeria spp. Clostridium perfringens. The broilers were distributed a completely randomized design poultry isolators (12 birds each), resulting 3 treatments: T1 (control, no challenge diet), T2 (challenged spp., followed by perfringens infection T3 treated amyloliquefaciens). They evaluated for period 29 d, divided into preinitial (1-7 d age), initial (8-21 d), growth (22-29 d) phases. Assessments body weight, weight gain, feed consumption, conversion conducted, along classification scores optical microscopy tract gastrointestinal. animals containing showed improved indicators performance. Additionally, (T3) had better score intestinal lesions compared other treatment groups. Therefore, consisting amyloliquefaciens could be considered an effective option disease prevention chickens.

Язык: Английский

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Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Сен. 25, 2023

Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since use antibiotics feed withdrawn, it imperative to find out suitable alternatives control NE. Baicalin-aluminum complex synthesized from baicalin, a flavonoid isolated Scutellaria baicalensis Georgi. The present study investigated effects baicalin-aluminum on virulence-associated traits and virulence genes expression C. CVCC2030, also evaluated vivo therapeutic effect results showed that inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity Caco-2 cells, downregulated encoding for clostridial toxins extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), sialidases (NanI, NanJ). Additionally, was found negatively regulate involved quorum sensing (QS) communication, including Agr QS system ( agrB , agrD ) VirS/R two-component regulatory virS virR ). In experiments, lightened intestinal lesions histological damage, pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) jejunal ileal tissues. Besides, alleviated upregulation Escherichia coli raised relative abundance Lactobacillus digesta. This suggests may be potential candidate against infection by inhibiting expression.

Язык: Английский

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An Overview of the Use and Applications of Limosilactobacillus fermentum in Broiler Chickens

Microorganisms, Год журнала: 2023, Номер 11(8), С. 1944 - 1944

Опубликована: Июль 29, 2023

The implementation of government regulations on antibiotic use, along with the public's concern for drug resistance, has strengthened interest in developing alternatives not only aimed at preserving animal production but also reducing effects pathogenic infections. Probiotics, particular, are considered microorganisms that induce health benefits host after consumption adequate amounts; they have been established as a potential strategy improving growth, especially by stimulating intestinal homeostasis. Probiotics commonly associated lactic acid bacteria, and Limosilactobacillus fermentum is well-studied species recognized its favorable characteristics, including adhesion to epithelial cells, antimicrobial compounds, activation receptors prompt transcription immune-associated genes. Recently, this used production. Different studies shown application L. strains improves ecosystem reduces caused potentially microorganisms. These revealed key insights into mechanisms behind actions exerted probiotic. In manuscript, we aim provide concise overview administration broiler chicken performance.

Язык: Английский

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