Augmentation of Fermentability and Bioavailability Characteristics of Wheat Bran via the Synergistic Interaction between Arabinoxylan-Specific Degrading Enzymes and Lactic Acid Bacteria DOI

Yanwei Xu,

Yihong Huang, Wei Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

To enhance the use of wheat bran in chicken feed, a solid-state fermentation approach was used with

Language: Английский

Phloretin supplementation ameliorates intestinal injury of broilers with necrotic enteritis by alleviating inflammation, enhancing antioxidant capacity, regulating intestinal microbiota, and producing plant secondary metabolites DOI Creative Commons
Mengjun Wu, Meng Peng,

J. Zang

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105187 - 105187

Published: April 1, 2025

Language: Английский

Citations

0
Dextran sulfate-coated curcumin nanocrystals for the treatment of DSS-induced ulcerative colitis in mice DOI
Y. Li, Huilin Ma, Huan Shi

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125428 - 125428

Published: March 1, 2025

Language: Английский

Citations

0
Cellular Immune Responses in Lymphoid Tissues of Broiler Chickens Experimentally Infected with Necrotic Enteritis–Producing Clostridium perfringens Strains DOI
Raveendra R. Kulkarni, Carissa Gaghan, Javid P. Mohammed

et al.

Avian Diseases, Journal Year: 2023, Volume and Issue: 67(2)

Published: June 29, 2023

Respuestas inmunes celulares en tejidos linfoides de pollos engorde infectados experimentalmente con cepas Clostridium perfringens productoras enteritis necrótica. Las respuestas del huésped contra (CP), el agente causante la necrótica (NE) pollos, son poco conocidas. En presente estudio, primero se analizó capacidad producción siete C. netB+ (CP5, CP18, CP26, CP64, CP67, CP68 y NCNE-1), utilizando un modelo infección experimental engorde. La evaluación las lesiones macroscópicas intestinales mostró que todas cepas, excepto CP5, podían producir necrótica, mientras CP26 CP64 produjeron relativamente más severas comparación los otros grupos. Posteriormente, evaluaron tonsilas cecales (CT), bolsa Fabricio bazo representan variaciones nivel virulencia, por ejemplo CP5 avirulenta, CP18 virulenta cepa virulenta. El análisis inmunofenotipado frecuencias macrófagos esplénicos o fueron significativamente altas controles no infectados, células T γd B grupos CP18. tenían número mayor esplénicas CD4+ CD8+ expresaban moléculas activación CD44 CD28, también una frecuencia estas activadas CP5. conjunto, estos hallazgos sugirieron celulares, incluida T, inducen selectivamente virulentas características este patógeno pueden influir resultado inmunidad

Citations

9
Association of Bacillus subtilis and Bacillus amyloliquefaciens: minimizes the adverse effects of necrotic enteritis in the gastrointestinal tract and improves zootechnical performance in broiler chickens DOI Creative Commons
Romário Alves Rodrigues, Leandro A.M. Silva, Heloisa Cristina Brugnera

et al.

Poultry Science, Journal Year: 2023, Volume and Issue: 103(3), P. 103394 - 103394

Published: Dec. 19, 2023

This study aimed to evaluate the efficiency and capacity of probiotic composed Bacillus subtilis amyloliquefaciens, in improving zootechnical performance broiler chickens challenged with Eimeria spp. Clostridium perfringens. The broilers were distributed a completely randomized design poultry isolators (12 birds each), resulting 3 treatments: T1 (control, no challenge diet), T2 (challenged spp., followed by perfringens infection T3 treated amyloliquefaciens). They evaluated for period 29 d, divided into preinitial (1-7 d age), initial (8-21 d), growth (22-29 d) phases. Assessments body weight, weight gain, feed consumption, conversion conducted, along classification scores optical microscopy tract gastrointestinal. animals containing showed improved indicators performance. Additionally, (T3) had better score intestinal lesions compared other treatment groups. Therefore, consisting amyloliquefaciens could be considered an effective option disease prevention chickens.

Language: Английский

Citations

8
Dietary Lactobacillus fermentum and Lactobacillus paracasei improve the intestinal health of broilers challenged with coccidia and Clostridium perfringens DOI Creative Commons
Peng Li,

Liyun Zheng,

QI Ya

et al.

Frontiers in Veterinary Science, Journal Year: 2022, Volume and Issue: 9

Published: Dec. 15, 2022

Necrotic enteritis (NE) is a great threat to the intestinal health of broilers, resulting in decreased growth performance and significant economic losses. Lactobacillus fermentum (LF) paracasei (LP) exert beneficial effects on health. The aim present study was investigate dietary LF LP broilers challenged with coccidia Clostridium perfringens (CCP). animal trial carried out using 336 (Ross 308) for 35 days completely randomized design. were divided into 4 groups based treatment as follows: control (CTR) group fed basal diet without CCP challenge challenge. CCP+LF CCP+LP by CCP, meanwhile, (1 × 109 CFU/g) supplemented diets, respectively. results showed that morphology negatively affected In addition, number digesta relative abundance Escherichia coli cecal increased. Besides, mRNA level IgA jejunum downregulated, transcript IL-8 upregulated Dietary failed improve However, they barrier function. able alleviate downregulation TGF-β spleen lesion scores compared group. Furthermore, alleviated upregulation reduced ileal digesta. conclusion, mitigate negative infection performance; however, strengthening alleviating inflammation.

Language: Английский

Citations

12
Protection of Broiler Chickens Against Necrotic Enteritis by Intrapulmonary Delivery of a Live Clostridium perfringens Vaccine Exploiting the Gut-Lung–Axis Concept DOI

Hemlata Gautam,

Khawaja Ashfaque Ahmed,

Iresha Subhasinghe

et al.

Avian Diseases, Journal Year: 2024, Volume and Issue: 68(3)

Published: Aug. 21, 2024

(CP)-induced necrotic enteritis (NE) is an economically important disease in the broiler chicken industry. The incidence of NE common 3-to-6-wk-old chickens, once maternal antibodies start declining. Developing effective vaccination strategy against NE, preferably delivering a single dose vaccine at hatch to protect chickens without booster vaccine, enormous challenge. objective this study was induce mucosal immunity intestines by intrapulmonary (IPL) delivery live CP hatch, exploiting gut-lung-axis (GLA) concept following

Language: Английский

Citations

2
Comparative Efficacy of Plant Extracts and Probiotics on Growth and Gut Health in Chickens with Necrotic Enteritis DOI Creative Commons
Ruiting Zhang, Jia Yang, Qingjie Wang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(22), P. 3312 - 3312

Published: Nov. 18, 2024

Necrotic enteritis (NE), caused by

Language: Английский

Citations

2
Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens DOI Creative Commons

Jin Liu,

Shuangqi Wu,

Honghao Zhao

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Sept. 25, 2023

Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since use antibiotics feed withdrawn, it imperative to find out suitable alternatives control NE. Baicalin-aluminum complex synthesized from baicalin, a flavonoid isolated Scutellaria baicalensis Georgi. The present study investigated effects baicalin-aluminum on virulence-associated traits and virulence genes expression C. CVCC2030, also evaluated vivo therapeutic effect results showed that inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity Caco-2 cells, downregulated encoding for clostridial toxins extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), sialidases (NanI, NanJ). Additionally, was found negatively regulate involved quorum sensing (QS) communication, including Agr QS system ( agrB , agrD ) VirS/R two-component regulatory virS virR ). In experiments, lightened intestinal lesions histological damage, pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) jejunal ileal tissues. Besides, alleviated upregulation Escherichia coli raised relative abundance Lactobacillus digesta. This suggests may be potential candidate against infection by inhibiting expression.

Language: Английский

Citations

6
Effects of challenge with Clostridium perfringens, Eimeria and both on ileal microbiota of yellow feather broilers DOI Creative Commons
Xin Feng,

Tonghao Li,

Hui Zhu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 1, 2022

In the poultry industry worldwide, Clostridium perfringens has been causing major economic loss as it can cause necrotic enteritis (NE). The coccidial infection considered most important predisposing factor of NE caused by C. perfringens. this study, we aimed to advance our knowledge on ileal microbiota yellow feather broilers under and/or Eimeria challenge. Total 80 healthy day old were randomly assigned four groups including: Control, challenge group (C. Per), (Cocc), and plus (Comb). On 14, Cocc Comb orally gavaged 1 ml PBS solution containing 25,000 oocysts brunetti maxima. Starting 17, Per 10 mL per bird (4 × 107 CFU/mL, ATCC® 13124™ Strain) every for 6 days. 16S rRNA gene sequencing was performed extracted DNA digesta samples. results showed that alone did not affect alpha diversity microbiome in but co-infection with significantly decreased microbiota. also relative abundance beneficial bacteria including Bacteroidetes at phylum level Faecalibacterium genus level. At species level, Candidatus Arthromitus challenged groups. This microbial shift information Perfringens provide reference data development therapeutic approaches yellow-feather broiler chickens.

Language: Английский

Citations

9
Immune Response Evaluation in Commercial Turkeys Affected with Clostridial Dermatitis DOI

Valeria Criollo,

Carissa Gaghan,

Feba Ann John

et al.

Avian Diseases, Journal Year: 2023, Volume and Issue: 67(1)

Published: March 15, 2023

Evaluación de la respuesta inmune en pavos comerciales afectados por dermatitis clostridial. La clostridial (CD), causada Clostridium septicum y perfringens, es una enfermedad emergente económicamente importante los caracterizada muerte súbitas necrótica. Se conoce poco acerca las respuestas inmunitarias En el presente estudio, se aisló C. durante un brote reciente, tejidos (piel, músculo bazo) recolectaron analizaron para determinar expresión genes inmunitarios junto con muestras aves clínicamente sanas. Los resultados mostraron que tenían niveles significativamente más altos transcritos IL-1β, IL-6, IFNγ, and iNOS piel, músculos bazo comparación también transcripción elevada del gene receptor tipo toll (TLR21) piel bazo, lo sugiere papel este reconocimiento inmunitario. IL-4 e IL-13 fue mayor afectadas. Aves adicionales mismas granjas afectadas sanas fueron examinadas serología revelaron anticuerpos séricos IgM IgY. Además, estimulación in vitro macrófagos MQ-NCSU condujo a regulación transcripcional al alza IL-1β mientras IL-10 reguló baja. superficial proteína MHC-II producción celular óxido nítrico aumentaron estimulados septicum, indica activación celular. Colectivamente, estos hallazgos sugieren huésped implican inflamatoria robusta, así como mediada citoquinas IL4/IL-13 pueden ayudar inmunidad anticuerpos.

Citations

5