Electrochemical Sensors based on Gold–Silver Core–Shell Nanoparticles Combined with a Graphene/PEDOT:PSS Composite Modified Glassy Carbon Electrode for Paraoxon-ethyl Detection

ACS Omega, Год журнала: 2024, Номер 9(2), С. 2896 - 2910

Опубликована: Янв. 2, 2024

Herein, a nonenzymatic detection of paraoxon-ethyl was developed by modifying glassy carbon electrode (GCE) with gold–silver core–shell (Au–Ag) nanoparticles combined the composite graphene poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT:PSS). These were synthesized using seed-growth method and characterized UV–vis spectroscopy high-resolution transmission electron microscopy (HR-TEM) techniques. Meanwhile, structural properties, surface morphology topography, electrochemical characterization Au–Ag core–shell/graphene/PEDOT:PSS analyzed infrared spectroscopy, field emission scanning (FE-SEM), atomic force (AFM), impedance (EIS) Moreover, proposed sensor for based on modified GCE demonstrates good electroanalytical performance when investigated cyclic voltammetry (CV), differential pulse (DPV), chronoamperometry It found that synergistic effect between graphene/PEDOT:PSS provides higher conductivity enhanced electrocatalytic activity at an optimum pH 7. At 7, shows linear range concentrations from 0.2 to 100 μM limit 10 nM high sensitivity 3.24 μA μM–1 cm–2. In addition, confirmed reproducibility, possibility being further as disposable electrode. This also displayed selectivity in presence several interfering species such diazinon, carbaryl, ascorbic acid, glucose, nitrite, sodium bicarbonate, magnesium sulfate. For practical applications, this employed determination real samples (fruits vegetables) showed no significant difference standard spectrophotometric technique. conclusion, might have potential be platform sensors pesticide detection.

Язык: Английский

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The looming threat of profenofos organophosphate and microbes in action for their sustainable degradation

Environmental Science and Pollution Research, Год журнала: 2024, Номер 31(10), С. 14367 - 14387

Опубликована: Янв. 30, 2024

Язык: Английский

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Enzyme-Immobilized Porous Crystals for Environmental Applications

Environmental Science & Technology, Год журнала: 2024, Номер 58(27), С. 11869 - 11886

Опубликована: Июнь 28, 2024

Developing efficient technologies to eliminate or degrade contaminants is paramount for environmental protection. Biocatalytic decontamination offers distinct advantages in terms of selectivity and efficiency; however, it still remains challenging when applied complex matrices. The main challenge originates from the instability difficult-to-separate attributes fragile enzymes, which also results issues compromised activity, poor reusability, low cost-effectiveness, etc. One viable solution harness biocatalysis environments known as enzyme immobilization, where a flexible tightly fixed solid carrier. In case reticular crystal utilized support, feasible engineer next-generation biohybrid catalysts functional complicated media. This can be interpreted by three aspects: (1) highly crystalline skeleton shield immobilized against external stressors. (2) porous network ensures high accessibility interior catalytic decontamination. And (3) adjustable unambiguous structure framework favors in-depth understanding interfacial interaction between enzyme, turn guide us designing active biocomposites. Review aims introduce this emerging technology involving pollutant degradation greenhouse gas (carbon dioxide) conversion, with emphasis on immobilization protocols diverse catalysis principles including single catalysis, cascades, photoenzyme-coupled catalysis. Additionally, remaining challenges forward-looking directions field are discussed. We believe that may offer useful biocatalytic contribute green sustainable manner will inspire more researchers at intersection environment science, biochemistry, materials science communities co-solve problems.

Язык: Английский

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A Review of the Reactivity of Phosphatase Controlled by Clays and Clay Minerals: Implications for Understanding Phosphorus Mineralization in Soils

Clays and Clay Minerals, Год журнала: 2023, Номер 71(2), С. 119 - 142

Опубликована: Апрель 1, 2023

Язык: Английский

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N-terminal PEGylation Enhances Organophosphorus Hydrolase Catalysis for a Promising Fast and Long-Acting Prophylactic Candidate

Journal of Hazardous Materials, Год журнала: 2025, Номер 488, С. 137336 - 137336

Опубликована: Янв. 25, 2025

Язык: Английский

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Unraveling the complexity of organophosphorus pesticides: Ecological risks, biochemical pathways and the promise of machine learning

The Science of The Total Environment, Год журнала: 2025, Номер 974, С. 179206 - 179206

Опубликована: Март 29, 2025

Язык: Английский

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Anchoring Effect-Induced Conformation Remodeling in Epoxy-Functionalized Covalent Organic Frameworks for Enhanced Enzymatic Efficiency

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Апрель 30, 2025

Covalent organic frameworks (COFs) with tunable structures and versatile chemical functionalities offer great promise for enzyme immobilization. This study explores the "anchoring effect" induced by covalent bonding, which significantly enhances enzymatic efficiency. Glucose oxidase (GOx) was covalently immobilized onto an epoxy-functionalized COF (oCOF-TATP) incorporating epoxy groups into framework. The immobilization approach increased loading from 26 mg/g (physical adsorption) to 40 mg/g. Secondary structure analysis revealed that binding promoted a shift α-helix β-sheet structure, more substantial effect than physical adsorption. Molecular dynamics simulations further confirmed anchoring enhanced structural rigidity exposed enzyme's active site. led 8.14% increase in accessible surface area 7.55% rise hydrogen bonding interactions. These improvements resulted significant catalytic enhancements, turnover rate (kcat) efficiency (kcat/KM) of GOx ⊂ oCOF-TATP reaching 197 388% free enzyme, respectively. In glucose oxidation assays, exhibited 115% product compared GOx, maintaining over 95% activity after six reuse cycles demonstrating superior stability under various conditions. represents novel mechanism optimizing performance operational stability.

Язык: Английский

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Engineering a Mesoporous Silicon Nanoparticle Cage to Enhance Performance of a Phosphotriesterase Enzyme for Degradation of VX Nerve Agent

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

Abstract The organophosphate (OP)‐hydrolyzing enzyme phosphotriesterase (PTE, variant L7ep‐3a) immobilized within a partially oxidized mesoporous silicon nanoparticle cage is synthesized and the catalytic performance of enzyme@nanoparticle construct for hydrolysis simulant, dimethyl p‐nitrophenyl phosphate (DMNP), live nerve agent VX benchmarked against free enzyme. In neutral aqueous buffer, optimized shows ≈2‐fold increase in rate DMNP turnover relative to Enzyme@nanoparticles with more hydrophobic surface chemistry interior pores show lower activity, suggesting importance hydration pore on performance. readily separated from neutralized agent; found retain activity through seven decontamination/recovery cycles. stabilizes thermal denaturing enzymatic (trypsin) degradation conditions When incorporated into topical gel formulation, PTE‐loaded nanoparticles high toward an ex vivo rabbit skin model. vitro acetylcholinesterase (AChE) assays human blood that decontaminates VX, preserving biological function AChE when exposed otherwise incapacitating dose.

Язык: Английский

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A novel phosphotriesterase hybrid nanoflower-hydrogel sensor equipped with a smartphone detector for real-time on-site monitoring of organophosphorus pesticides

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 276, С. 133979 - 133979

Опубликована: Июль 17, 2024

Язык: Английский

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Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7822 - 7822

Опубликована: Июль 17, 2024

Mining of organophosphorous (OPs)-degrading bacterial enzymes in collections known strains and natural biotopes are important research fields that lead to the isolation novel OP-degrading enzymes. Then, implementation strategies methods protein engineering nanobiotechnology allow large-scale production enzymes, displaying improved catalytic properties for medical uses protection environment. For applications, enzyme formulations must be stable bloodstream upon storage not susceptible induce iatrogenic effects. This, particular, includes nanoencapsulation bioscavengers origin. In application field bioremediation, these play a crucial role environmental cleanup by initiating degradation OPs, such as pesticides, contaminated environments. microbial cell configuration, can break down chemical bonds OPs usually convert them into less toxic metabolites through biotransformation process or contribute their complete mineralization. purified state, they exhibit higher pollutant efficiencies ability operate under different conditions. Thus, this review provides clear overview current knowledge about applications OP-reacting It presents works focusing on use various bioremediation mitigate pollution medicine alternative therapeutic means against OP poisoning.

Язык: Английский

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