Polylactic Acid Micro/Nanoplastic Exposure Induces Male Reproductive Toxicity by Disrupting Spermatogenesis and Mitochondrial Dysfunction in Mice

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Although considered an "eco-friendly" biodegradable plastic, polylactic acid (PLA) microplastic (PLA-MP) poses a growing concern for human health, yet its effects on male reproductive function remain underexplored. This study investigated the toxicity of PLA in mice and potential mechanisms. To this end, our vivo vitro experiments demonstrated that after degradation digestive system, significant number PLA-MP-derived nanoparticles could penetrate blood–testis barrier (BTB) localize within spermatogenic microenvironment. Mice exposed to PLA-MPs long time exhibited toxicity, evidenced by decreased sperm concentration motility, increased deformity rates, disrupted sex hormone levels. Further analysis revealed impaired BTB, induced mitochondrial dysfunction testes, triggered oxidative stress through excessive ROS production from mitochondria, leading further testicular damage. Notably, nanoplastics internalized sheath structure sperm, causing dose-dependent impairments function. Transcriptome analyses indicated spermatogenesis inhibiting expression key mRNA involved process. Collectively, findings highlight toxic effect damaging BTB impairing function, which provides insights into toxicological implications microplastics mammalian fertility.

Язык: Английский

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In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics

Journal of Hazardous Materials, Год журнала: 2024, Номер 469, С. 134030 - 134030

Опубликована: Март 13, 2024

Continuous exposure to plastic pollutants may have serious consequences on human health. However, most toxicity assessments focus non-environmentally relevant particles and rarely investigate long-term effects such as cancer induction. The present study assessed the carcinogenic potential of two secondary nanoplastics: polyethylene terephthalate (PET) generated from bottles, a biodegradable polylactic acid material, respective examples environmentally existing new bioplastics. Pristine polystyrene nanoplastics were also included for comparison. A broad concentration range (6.25 – 200 μg/mL) each nanoplastic was tested in both initiation promotion conditions regulatory assessment-accepted vitro Bhas 42 cell transformation assay. Parallel cultures allowed confirmation efficient cellular internalisation three nanoplastics. Cell growth enhanced by assay, PET conditions. Moreover, number transformed foci significantly increased only highest which showed dose-dependency, indicating that nano can act non-genotoxic tumour promotor. Together, these findings support risk assessment raise concerns regarding whether real-life co-exposure other environmental result synergistic capacities. Secondary (NPLs) waste degradation bioaccumulate tissues cause cancer. Currently, information NPL carcinogenicity remains scarce. By using we first time NPLs, bioplastic, widely spread petroleum-based alternative, respectively. Notably, accelerated bioplastics has been associated with an release NPLs into environment. data will serve plastics.

Язык: Английский

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Polylactic acid nanoplastics (PLA-NPLs) induce adverse effects on an in vitro model of the human lung epithelium: The Calu-3 air-liquid interface (ALI) barrier

Journal of Hazardous Materials, Год журнала: 2024, Номер 475, С. 134900 - 134900

Опубликована: Июнь 13, 2024

The expected increments in the production/use of bioplastics, as an alternative to petroleum-based plastics, require a deep understanding their potential environmental and health hazards, mainly nanoplastics (NPLs). Since one important exposure route NPLs is through inhalation, this study aims determine fate effects true-to-life polylactic acid (PLA-NPLs), using vitro Calu-3 model bronchial epithelium, under air-liquid interphase conditions. To harmful PLA-NPLs more realistic scenario, both acute (24 h) long-term (1 2 weeks) exposures were used. Flow cytometry results indicated that internalized easily barrier (∼10 % at 24 h ∼40 after weeks), which affected expression tight-junctions formation (∼50 less vs control) mucus secretion control), measured by immunostaining. Interestingly, significant genotoxic (DNA breaks) detected comet assay, with being marked than ones (7.01 4.54 DNA damage). When array cellular proteins including cytokines, chemokines, growth factors used, over-expression was found (∼20 5 exposure). Overall, these described hazards posed PLA-NPLs, relevant scenarios, highlighting advantages used epithelium tissue damage, signaling endpoints related inflammation.

Язык: Английский

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Kinetics and toxicity of nanoplastics in ex vivo exposed human whole blood as a model to understand their impact on human health

The Science of The Total Environment, Год журнала: 2024, Номер 948, С. 174725 - 174725

Опубликована: Июль 14, 2024

The ubiquitous presence of nanoplastics (NPLs) in the environment is considered great health concern. Due to their size, NPLs can cross both intestinal and pulmonary barriers and, consequently, blood compartment expected. Understanding interactions between human components required. In this study, simulate more adequate real exposure conditions, whole healthy donors was exposed five different NPLs: three polystyrene approximately 50 nm (aminated PS-NH

Язык: Английский

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Drosophila melanogaster as a tractable eco-environmental model to unravel the toxicity of micro- and nanoplastics

Environment International, Год журнала: 2024, Номер 192, С. 109012 - 109012

Опубликована: Сен. 17, 2024

Язык: Английский

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Ecotoxicity of Biodegradable Microplastics and Bio-based Microplastics: A Review of in vitro and in vivo Studies

Environmental Management, Год журнала: 2024, Номер unknown

Опубликована: Дек. 27, 2024

Язык: Английский

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The comparative toxicity of biobased, modified biobased, biodegradable, and petrochemical-based microplastics on the brackish water flea Diaphanosoma celebensis

The Science of The Total Environment, Год журнала: 2024, Номер 944, С. 173747 - 173747

Опубликована: Июнь 3, 2024

Язык: Английский

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Necroecological Trophic Transfer of Microplastics: Insights into the Ecotoxicity of Petroleum-Derived and Biodegradable Polymers

Archives of Environmental Contamination and Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 8, 2025

Язык: Английский

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The efficient prediction of inflammatory osteolysis caused by polylactic acid through network toxicology and molecular docking strategy

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 291, С. 117876 - 117876

Опубликована: Фев. 1, 2025

Polylactic acid (PLA), as a bioplastic, is extensively utilized in bone tissue engineering for its biocompatibility, adaptability and affordability. However, the toxicological research of PLA still limited. The hydrolysis products induced inflammatory response which caused osteolysis mediated by oxidative damage through recruitment macrophages accumulation foreign body multinucleated giant cells, ultimately leading to failure regeneration. lack effective treatments highlights importance finding new therapies. This study systematically investigated potential molecular mechanisms PLA-induced employing network toxicology docking techniques. We first conducted toxicology-based assessment according structure PLA. result from integrating screening targets multiple databases identified 126 associated with osteolysis, then an interaction diagram was constructed. Gene ontology (GO)/KEGG enrichment analysis clarified that may cause via metabolic pathways cancer, well lipid atherosclerosis. Further STRING Cytoscape software screened 25 core including HSP90AA1, AKT1, SRC, STAT1 FYN. found enriched highly correlated covered 18 targets, supporting scientific hypothesis induces osteolysis. Moreover, results confirmed displayed strong binding ability formed stable binding. Taken together, this not only revealed biological mechanism but also provided evidence future prevention treatment inflammation.

Язык: Английский

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Poly(lactic acid) nanoplastics through laser ablation: Establishing a reference model for mimicking biobased nanoplastics in aquatic environments.

Environmental Science Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

In response to the limited environmentally relevant biobased nanoplastic models, formation of poly(lactic acid) nanoplastics with comparable properties ones deriving from environmental ageing debris, is presented.

Язык: Английский

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