LncRNA FOXD1-AS1 regulates pancreatic cancer stem cell properties and 5-FU resistance by regulating the miR-570-3p/SPP1 axis as a ceRNA DOI Creative Commons
Liu Ouyang, Minmin Sun,

Ping-sheng Zhou

и другие.

Cancer Cell International, Год журнала: 2024, Номер 24(1)

Опубликована: Янв. 2, 2024

Abstract Cancer stem cells (CSCs) play a pivotal role in the pathogenesis of human cancers. Previous studies have highlighted long non-coding RNA (lncRNA) modulating stemness CSCs. In our investigation, we identified an upregulation lncRNA FOXD1-AS1 The enforced expression promotes tumorigenesis and self-renewal pancreatic cancer Conversely, knockdown inhibits Furthermore, findings reveal that enhances CSCs by up-regulating osteopontin/secreted phosphoprotein 1(SPP1) acting as ceRNA to sponge miR-570-3p (PC) Additionally, depleted exhibit heightened sensitivity 5-FU-indued cell growth inhibition apoptosis. Analysis patient-derived xenografts (PDX) indicates low level may serve predictor 5-FU benefits PC patients. Moreover, introduction SPP1 can reverse FOXD1-AS1-knockdown 5-FU-induced Importantly, molecular indicated elevated levels lncRNAFOXD1-AS1 are facilitated through METTL3 YTHDF1-dependent m 6 A methylation. summary, results underscore critical functions CSCs, positioning promising therapeutic target for PC.

Язык: Английский

Hypoxia inducible lncRNA-CBSLR modulates ferroptosis through m6A-YTHDF2-dependent modulation of CBS in gastric cancer DOI Creative Commons
Hui Yang, Yiren Hu, Mingzhe Weng

и другие.

Journal of Advanced Research, Год журнала: 2021, Номер 37, С. 91 - 106

Опубликована: Окт. 6, 2021

Tumors are usually refractory to anti-cancer therapeutics under hypoxic conditions. However, the underlying molecular mechanism remains be elucidated. Our study intended identify hypoxia inducible lncRNAs and their biological function in gastric cancer (GC). Differentially expressed were determined by microarray analysis between GC cells exposed (1% O2) normoxia (21% for 24 h. The expression level of CBSLR was manipulated several cell lines perform analyses both vitro vivo. We identified a hypoxia-induced lncRNA-CBSLR that protected from ferroptosis, leading chem-resistance. Mechanically, interacted with YTHDF2 form CBSLR/YTHDF2/CBS signaling axis decreased stability CBS mRNA enhancing binding m6A-modified coding sequence (CDS) mRNA. Furthermore, levels, methylation ACSL4 protein reduced, polyubiquitination degradation ACSL4. This, turn, pro-ferroptosis phosphatidylethanolamine (PE) (18:0/20:4) PE (18:0/22:4) content contributed ferroptosis resistance. Notably, is upregulated, whereas downregulated tissues compared matched normal tissues; patients high CBSLR/low levels have worse clinical outcome poorer response chemotherapy. reveals novel how HIF1α/CBSLR modulates ferroptosis/chemoresistance GC, illuminating potential therapeutic targets tumors.

Язык: Английский

Процитировано

182
Long non-coding RNAs in the doxorubicin resistance of cancer cells DOI

Saeed Ashrafizaveh,

Milad Ashrafizadeh, Ali Zarrabi

и другие.

Cancer Letters, Год журнала: 2021, Номер 508, С. 104 - 114

Опубликована: Март 23, 2021

Язык: Английский

Процитировано

157
Molecular Landscape of LncRNAs in Prostate Cancer: A focus on pathways and therapeutic targets for intervention DOI Creative Commons
Sepideh Mirzaei, Mahshid Deldar Abad Paskeh,

Elena Okina

и другие.

Journal of Experimental & Clinical Cancer Research, Год журнала: 2022, Номер 41(1)

Опубликована: Июль 1, 2022

Abstract Background One of the most malignant tumors in men is prostate cancer that still incurable due to its heterogenous and progressive natures. Genetic epigenetic changes play significant roles development. The RNA molecules with more than 200 nucleotides length are known as lncRNAs these factors do not encode protein. They regulate gene expression at transcriptional, post-transcriptional levels. LncRNAs vital biological functions cells pathological events, hence their undergoes dysregulation. Aim review role alterations development emphasized here. Therefore, were chosen for this purpose level interaction other signaling networks progression examined. Key scientific concepts aberrant has been well-documented rate tumor regulated via affecting STAT3, NF-κB, Wnt, PI3K/Akt PTEN, among molecular pathways. Furthermore, radio-resistance chemo-resistance features cells. Overexpression tumor-promoting such HOXD-AS1 CCAT1 can result drug resistance. Besides, induce immune evasion upregulating PD-1. Pharmacological compounds quercetin curcumin have applied targeting lncRNAs. siRNA tool reduce thereby suppressing progression. Prognosis diagnosis clinical course be evaluated by exosomal lncRNA-p21 investigated serum patients a reliable biomarker.

Язык: Английский

Процитировано

129
Characteristics of the cancer stem cell niche and therapeutic strategies DOI Creative Commons
Feng Ju, Manar Atyah,

Nellie Horstmann

и другие.

Stem Cell Research & Therapy, Год журнала: 2022, Номер 13(1)

Опубликована: Июнь 3, 2022

Distinct regions harboring cancer stem cells (CSCs) have been identified within the microenvironment of various tumors, and as in case their healthy counterparts, these anatomical are termed "niche." Thus far, a large volume studies shown that CSC niches take part maintenance, regulation renewal, differentiation plasticity CSCs. In this review, we summarize discuss latest findings regarding niche morphology, physical terrain, main signaling pathways interactions them. The cellular molecular components CSCs also involve genetic epigenetic modulations mediate support ultimately leading to progression. It suggests crosstalk between plays an important role therapy resistance recurrence. addition, updated diverse therapeutic strategies different cancers basic research clinical trials review. Understanding complex heterogeneity is necessary pre-requisite for designing superior target CSC-specific factors and/or niche.

Язык: Английский

Процитировано

79
Cancer-associated fibroblasts facilitate premetastatic niche formation through lncRNA SNHG5-mediated angiogenesis and vascular permeability in breast cancer DOI Creative Commons
Huan Zeng, Yixuan Hou, Xinyue Zhou

и другие.

Theranostics, Год журнала: 2022, Номер 12(17), С. 7351 - 7370

Опубликована: Янв. 1, 2022

Background: Metastasis is the leading cause of death in patients with breast cancer (BC). Primary tumors create a premetastatic niche (PMN) secondary organs for subsequent metastases. Cancer-associated fibroblasts (CAFs) are predominant stromal component tumor microenvironment and serve as major contributor to metastasis. However, function mechanism primary CAFs remain unclear BC. Methods: We investigated expression profiles lncRNAs pairs NFs derived from tissues using lncRNA microarray. The levels lncSNHG5, ZNF281, IGF2BP2, CCL2 CCL5 were assessed by qRT-PCR; protein related genes (e.g., CCL2, CCL5) analyzed western blotting and/or ELISA immortalized clinical samples. Tubule formation three-dimensional sprouting assays tissue fluorescence staining conducted investigate angiogenesis. In vitro permeability assays, trans-endothelial invasion vivo examine vascular permeability. regulatory lncSNHG5 was RNA sequencing, fluorescent situ hybridization, cellular fractionation assay, mass spectrometry, pull-down, immunoprecipitation, gene-specific m6A chromatin dual luciferase reporter assay actinomycin D treatment NFs. Results: LncSNHG5 highly expressed played an essential role promoting angiogenesis leakiness through regulation ZNF281 CAFs. enhanced mRNA stability binding reader IGF2BP2. Enhanced transcriptionally regulated activate P38 MAPK signaling endothelial cells. High associated metastasis poor prognosis BC patients. inhibitors RS102895, marasviroc cenicriviroc inhibited PMN blocking CCL2/CCR2 CCL5/CCR5. lncSNHG5-ZNF281-CCL2/CCL5 axis plays inducing promote Conclusions: Our work demonstrates that its downstream ZNF281-CCL2/CCL5 play crucial may potential targets diagnosis

Язык: Английский

Процитировано

77
Regulation and signaling pathways in cancer stem cells: implications for targeted therapy for cancer DOI Creative Commons
Zhen Zeng,

Minyang Fu,

Yuan Hu

и другие.

Molecular Cancer, Год журнала: 2023, Номер 22(1)

Опубликована: Окт. 18, 2023

Abstract Cancer stem cells (CSCs), initially identified in leukemia 1994, constitute a distinct subset of tumor characterized by surface markers such as CD133, CD44, and ALDH. Their behavior is regulated through complex interplay networks, including transcriptional, post-transcriptional, epigenetic, microenvironment (TME), epithelial-mesenchymal transition (EMT) factors. Numerous signaling pathways were found to be involved the regulatory network CSCs. The maintenance CSC characteristics plays pivotal role driving CSC-associated metastasis conferring resistance therapy. Consequently, CSCs have emerged promising targets cancer treatment. To date, researchers developed several anticancer agents tailored specifically target CSCs, with some these treatment strategies currently undergoing preclinical or clinical trials. In this review, we outline origin biological explore networks governing discuss implicated investigate influential factors contributing therapy Finally, offer insights into designed eliminate

Язык: Английский

Процитировано

64
Predict lncRNA-drug associations based on graph neural network DOI Creative Commons
Peng Xu, Chuchu Li, Jiaqi Yuan

и другие.

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Апрель 26, 2024

LncRNAs are an essential type of non-coding RNAs, which have been reported to be involved in various human pathological conditions. Increasing evidence suggests that drugs can regulate lncRNAs expression, makes it possible develop as therapeutic targets. Thus, developing

Язык: Английский

Процитировано

28
lncRNA and breast cancer: Progress from identifying mechanisms to challenges and opportunities of clinical treatment DOI Creative Commons
Xin Jin, Wei Du, Wentao Huang

и другие.

Molecular Therapy — Nucleic Acids, Год журнала: 2021, Номер 25, С. 613 - 637

Опубликована: Авг. 19, 2021

Breast cancer is a malignant tumor that has high mortality rate and mostly occurs in women. Although significant progress been made the implementation of personalized treatment strategies for molecular subtypes breast cancer, therapeutic response often not satisfactory. Studies have reported long non-coding RNAs (lncRNAs) are abnormally expressed closely related to occurrence development cancer. In addition, tissue cell-type specificity makes lncRNAs particularly attractive as diagnostic biomarkers, prognostic factors, specific targets. Therefore, an in-depth understanding regulatory mechanisms essential developing new strategies. this review, we systematically elucidate general characteristics, potential mechanisms, targeted therapy discuss emerging functions Additionally, also highlight advantages challenges using biomarkers diagnosis or targets drug resistance present future perspectives clinical practice.

Язык: Английский

Процитировано

82
LncTarD 2.0: an updated comprehensive database for experimentally-supported functional lncRNA–target regulations in human diseases DOI Creative Commons

Hongying Zhao,

Xiangzhe Yin,

Haotian Xu

и другие.

Nucleic Acids Research, Год журнала: 2022, Номер 51(D1), С. D199 - D207

Опубликована: Ноя. 2, 2022

Abstract An updated LncTarD 2.0 database provides a comprehensive resource on key lncRNA–target regulations, their influenced functions and lncRNA-mediated regulatory mechanisms in human diseases. is freely available at (http://bio-bigdata.hrbmu.edu.cn/LncTarD or https://lnctard.bio-database.com/). was with several new features, including (i) an increased number of disease-associated lncRNA entries, where the current release 8360 419 disease subtypes 1355 lncRNAs; (ii) predicted 3312 out regulations as potential diagnostic therapeutic biomarkers circulating tumor cells (CTCs); (iii) addition 536 new, experimentally supported that modulate properties cancer stem cells; (iv) clinical application section 2894 for application. Importantly, RNA-seq/microarray single-cell web tools customizable analysis visualization tool used to mining both tissue samples CTCs blood samples. The provide annotation from perspectives pan-cancer cancer-specific level. will be useful investigation deregulation disease.

Язык: Английский

Процитировано

45
Disheveled3 enhanced EMT and cancer stem-like cells properties via Wnt/β-catenin/c-Myc/SOX2 pathway in colorectal cancer DOI Creative Commons
Zhengguang Li, Zhirong Yang, Wei Liu

и другие.

Journal of Translational Medicine, Год журнала: 2023, Номер 21(1)

Опубликована: Май 5, 2023

Epithelial-to-mesenchymal transition (EMT) and cancer stem-like cells (CSLCs) play crucial role in tumor metastasis drug-resistance. Disheveled3 (DVL3) is involved malignant behaviors of cancer. However, the potential mechanism DVL3 remain elusive EMT CSLCs colorectal (CRC).

Язык: Английский

Процитировано

32