Vaccines,
Год журнала:
2022,
Номер
10(12), С. 2138 - 2138
Опубликована: Дек. 13, 2022
Early
and
effective
diagnosis
of
cancer
is
decisive
for
its
proper
management.
In
this
context
biomarker-based
budding
as
one
the
promising
ways
early
detection,
disease
progression
monitoring,
therapy.
Integration
Biosensing
devices
with
different
metallic/nonmetallic
nanoparticles
offers
amplification
multiplexing
capabilities
simultaneous
detection
biomarkers
(CB’s).
This
study
provides
a
comprehensive
analysis
most
recent
designs
fabrication
methodologies
designed
developing
electrochemical
biosensors
(EB)
cancers.
The
role
in
therapeutics
also
discussed.
Abstract
Osteoporosis
is
a
widely
observed
condition
characterized
by
the
systemic
deterioration
of
bone
mass
and
microarchitecture,
which
increases
patient
susceptibility
to
fragile
fractures.
The
intricate
mechanisms
governing
homeostasis
are
substantially
impacted
extracellular
vesicles
(EVs),
play
crucial
roles
in
both
pathological
physiological
contexts.
EVs
derived
from
various
sources
exert
distinct
effects
on
osteoporosis.
Specifically,
released
osteoblasts,
endothelial
cells,
myocytes,
mesenchymal
stem
cells
contribute
formation
due
their
unique
cargo
proteins,
miRNAs,
cytokines.
Conversely,
secreted
osteoclasts
immune
promote
resorption
inhibit
formation.
Furthermore,
use
as
therapeutic
modalities
or
biomaterials
for
diagnosing
managing
osteoporosis
promising.
Here,
we
review
current
understanding
impact
homeostasis,
including
classification
biogenesis
regulatory
present
an
overview
latest
research
progress
treating
using
EVs.
Finally,
discuss
challenges
prospects
translational
Molecular Therapy — Nucleic Acids,
Год журнала:
2023,
Номер
34, С. 102040 - 102040
Опубликована: Сен. 26, 2023
Therapeutic
genome
editing
has
the
potential
to
cure
diseases
by
directly
correcting
genetic
mutations
in
tissues
and
cells.
Recent
progress
CRISPR-Cas9
systems
led
breakthroughs
gene
tools
because
of
its
high
orthogonality,
versatility,
efficiency.
However,
safe
effective
administration
target
organs
patients
is
a
major
hurdle.
Extracellular
vesicles
(EVs)
are
endogenous
membranous
particles
secreted
spontaneously
all
They
key
actors
cell-to-cell
communication,
allowing
exchange
select
molecules
such
as
proteins,
lipids,
RNAs
induce
functional
changes
recipient
Recently,
EVs
have
displayed
their
for
trafficking
system
during
or
after
formation.
In
this
review,
we
highlight
recent
developments
EV
loading,
surface
functionalization,
strategies
increasing
efficiency
delivering
tissues,
organs,
cells
eventual
use
therapies.
Cells,
Год журнала:
2024,
Номер
13(4), С. 336 - 336
Опубликована: Фев. 13, 2024
Glioblastoma
(GB)
is
a
rare
but
extremely
aggressive
brain
tumor
that
significantly
impacts
patient
outcomes,
affecting
both
duration
and
quality
of
life.
The
protocol
established
by
Stupp
colleagues
in
2005,
based
on
radiotherapy
chemotherapy
with
Temozolomide,
following
maximum
safe
surgical
resection
remains
the
gold
standard
for
GB
treatment;
however,
it
evident
nowadays
extreme
intratumoral
intertumoral
heterogeneity,
as
well
invasiveness
tendency
to
recur,
are
not
compatible
routine
unfortunately
ineffective
treatment.
This
review
article
summarizes
main
challenges
search
new
valuable
therapies
focuses
impact
extracellular
vesicle
(EV)
research
exploitation
may
have
field.
EVs
natural
particles
delimited
lipidic
bilayer
filled
functional
cellular
content
released
uptaken
cells
key
means
cell
communication.
Furthermore,
stable
body
fluids
tolerated
immune
system,
able
cross
physiological,
interspecies,
interkingdom
barriers
target
specific
cells,
releasing
inherent
or
externally
loaded
functionally
active
molecules.
Therefore,
potential
be
ideal
allies
fight
against
improve
prognosis
patients.
present
work
describes
preclinical
results
obtained
so
far
use
treatment,
focusing
EV
sources
molecular
cargo
used
various
studies,
primarily
vivo.
Finally,
SWOT
analysis
performed,
highlighting
advantages
pitfalls
developing
EV-based
therapeutic
strategies.
also
suggests
directions
explore
realize
possibility
exploiting
treatment
GB.
European journal of medical research,
Год журнала:
2024,
Номер
29(1)
Опубликована: Июнь 28, 2024
Abstract
Cancer,
a
prevalent
and
complex
disease,
presents
significant
challenge
to
the
medical
community.
It
is
characterized
by
irregular
cell
differentiation,
excessive
proliferation,
uncontrolled
growth,
invasion
of
nearby
tissues,
spread
distant
organs.
Its
progression
involves
interplay
several
elements
processes.
Extracellular
vesicles
(EVs)
serve
as
critical
intermediaries
in
intercellular
communication,
transporting
molecules
such
lipids,
RNA,
membrane,
cytoplasmic
proteins
between
cells.
They
significantly
contribute
progression,
development,
dissemination
primary
tumors
facilitating
exchange
information
transmitting
signals
that
regulate
tumor
growth
metastasis.
However,
EVs
do
not
have
singular
impact
on
cancer;
instead,
they
play
multifaceted
dual
role.
Under
specific
circumstances,
can
impede
influence
cancer
delivering
oncogenic
factors
or
triggering
an
immune
response.
Furthermore,
from
different
sources
demonstrate
distinct
advantages
inhibiting
cancer.
This
research
examines
biological
characteristics
their
involvement
development
establish
theoretical
foundation
for
better
understanding
connection
Here,
we
discuss
potential
various
therapy,
well
current
status
future
prospects
engineered
developing
more
effective
treatments.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(24), С. 15827 - 15827
Опубликована: Дек. 13, 2022
Neutrophils
represent
the
most
abundant
cell
type
of
leukocytes
in
human
blood
and
have
been
considered
a
vital
player
innate
immune
system
first
line
defense
against
invading
pathogens.
Recently,
several
studies
showed
that
neutrophils
play
an
active
role
response
during
cancer
development.
They
exhibited
both
pro-oncogenic
anti-tumor
activities
under
influence
various
mediators
tumor
microenvironment.
can
be
divided
into
subpopulations,
thus
contradicting
traditional
concept
as
homogeneous
population
with
specific
function
immunity
opening
new
horizons
for
therapy.
Despite
promising
achievements
this
field,
full
understanding
tumor–neutrophil
interplay
is
currently
lacking.
In
review,
we
try
to
summarize
current
view
on
neutrophil
heterogeneity
cancer,
discuss
different
communication
pathways
between
tumors
neutrophils,
focus
implementation
these
findings
develop
neutrophil-based
therapies.
Journal of Clinical Medicine,
Год журнала:
2022,
Номер
11(23), С. 6892 - 6892
Опубликована: Ноя. 22, 2022
Tumor
cells
actively
incorporate
molecules
(e.g.,
proteins,
lipids,
RNA)
into
particles
named
extracellular
vesicles
(EVs).
Several
groups
have
demonstrated
that
EVs
can
be
transferred
to
target
(recipient)
cells,
making
an
important
means
of
intercellular
communication.
Indeed,
are
able
modulate
the
functions
by
reprogramming
signaling
pathways.
In
a
cancer
context,
promote
formation
supportive
tumor
microenvironment
(TME)
and
(pre)metastatic
niches.
Recent
studies
revealed
immune
their
secretome,
including
EVs,
changes
in
TME
immunosuppressive
natural
killer,
dendritic
T
B
monocytes,
macrophages)
allow
establish
propagate.
Despite
growing
knowledge
on
roles
as
modulators
response/escape,
translation
clinical
practice
remains
its
early
stages,
hence
requiring
improved
translational
research
field.
Here,
we
comprehensively
review
current
most
recent
evasion
immunosuppression
both
solid
tumors
hematological
malignancies.
We
also
highlight
utility
EV-mediated
targeting
EV-engineering.
Importantly,
discuss
controversial
role
biology,
limitations
future
perspectives
further
EV
practice.
Biological Research,
Год журнала:
2024,
Номер
57(1)
Опубликована: Янв. 3, 2024
Abstract
Background
Tumor-derived
small
extracellular
vesicles
(sEVs)
can
promote
tumorigenic
and
metastatic
capacities
in
less
aggressive
recipient
cells
mainly
through
the
biomolecules
their
cargo.
However,
despite
recent
advances,
specific
molecules
orchestrating
these
changes
are
not
completely
defined.
Lactadherin
is
a
secreted
glycoprotein
typically
found
milk
fat
globule
membrane.
Its
overexpression
has
been
associated
with
increased
tumorigenesis
metastasis
breast
cancer
(BC)
other
tumors.
neither
its
presence
sEVs
by
BC
cells,
nor
role
sEV-mediated
intercellular
communication
have
described.
The
present
study
focused
on
of
lactadherin-containing
from
MDA-MB-231
triple-negative
(TNBC)
(sEV-MDA231)
promotion
pro-metastatic
non-tumorigenic
non-metastatic
vitro,
as
well
murine
model
peritoneal
carcinomatosis.
Results
We
show
that
lactadherin
it
higher
sEV-MDA231
compared
cell-secreted
measured
ELISA.
Incubation
increases
migration
and,
to
some
extent,
tumoroid
formation
capacity
but
anchorage-independent
growth.
Remarkably,
blockade
results
significant
decrease
those
vitro.
Similarly,
intraperitoneally
treatment
mice
greatly
increase
malignant
ascites
tumor
micronodules,
effects
were
significantly
inhibited
when
was
previously
blocked
sEV-MDA231.
Conclusions
As
our
knowledge,
provides
first
evidence
promoter
of:
(i)
ii)
nodules.
These
understanding
which
be
used
therapeutic
option
for
malignancies.
Membranes,
Год журнала:
2022,
Номер
12(12), С. 1244 - 1244
Опубликована: Дек. 8, 2022
Extracellular
vesicles
(EV)
are
membrane
surrounded
by
a
lipid
bilayer
and
include
microvesicles,
apoptotic
bodies,
exosomes,
exomeres.
Exosome-encapsulated
microRNAs
(miRNAs)
released
from
cancer
cells
involved
in
the
proliferation
metastasis
of
tumor
via
angiogenesis.
On
other
hand,
mesenchymal
stem
cell
(MSC)
therapy,
which
is
being
employed
regenerative
medicine
owing
to
ability
MSCs
differentiate
into
various
cells,
due
humoral
factors,
including
messenger
RNA
(mRNA),
miRNAs,
proteins,
lipids,
encapsulated
exosomes
derived
transplanted
cells.
New
treatments
that
advocate
cell-free
therapy
using
MSC-derived
will
significantly
improve
clinical
practice.
Therefore,
highly
purified
perform
their
original
functions
desirable.
In
this
review,
we
summarized
advances
purification,
modification,
application
EVs
as
novel
strategies
treat
some
diseases.
