HES1 revitalizes the functionality of aged adipose-derived stem cells by inhibiting the transcription of STAT1 DOI Creative Commons
Chengcheng Li, Sen Ren, Chengqi Yan

и другие.

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 5, 2024

The effectiveness of adipose-derived stem cells (ADSCs) in therapy diminishes with age. It has been reported that transcription factors (TFs) play a crucial role the aging and functionality cells. Nevertheless, there is limited understanding regarding involvement TFs mechanism ADSCs. RNA sequencing (RNA-seq) was utilized to discern differentially expressed genes ADSCs obtained from donors varying ages. exhibiting significant variations across age groups were identified subsequently validated. manipulated exhibit either enhanced expression or reduced levels HES1 STAT1 via lentivirus transfection small interfering (siRNA) techniques. impact these genetic alterations on ADSCs' proliferation, migration, cellular senescence assessed using EdU, transwell, senescence-activated β-galactosidase (SA-β-gal) staining assays. DNA sequences bound by investigated through CUT & Tag assay. Lastly, therapeutic efficacy aged overexpression evaluated skin injury model male Sprague-Dawley rats. 678 showed differential between young old (Y-ADSCs O-ADSCs), 47 being TFs. Notably, TF hairy enhancer split 1 (HES1) notably donors. Introducing resulted improved function suppression senescence, while reducing had opposite effect. Mechanistically, found interact promoter region another TF, signal transducer activator (STAT1), inhibit its transcription. Knocking down could fully reverse negative effects caused decreased ADSCs, leading reduction secretion pro-inflammatory cytokines such as TNF-α, IL-6, IL-8. Ultimately, restoring demonstrated potential promoting wound healing. acts an inhibitor progression modulation expression, suggesting promising avenue for rejuvenating senescent improving

Язык: Английский

Stress, epigenetics, and aging: Unraveling the intricate crosstalk DOI Creative Commons
Zeming Wu, Jing Qu, Weiqi Zhang

и другие.

Molecular Cell, Год журнала: 2023, Номер 84(1), С. 34 - 54

Опубликована: Ноя. 13, 2023

Язык: Английский

Процитировано

54
Emerging epigenetic insights into aging mechanisms and interventions DOI
Zeming Wu, Weiqi Zhang, Jing Qu

и другие.

Trends in Pharmacological Sciences, Год журнала: 2024, Номер 45(2), С. 157 - 172

Опубликована: Янв. 11, 2024

Язык: Английский

Процитировано

20
Aging and the emerging role of cellular senescence in osteoarthritis DOI
Brian O. Diekman, Richard F. Loeser

Osteoarthritis and Cartilage, Год журнала: 2023, Номер 32(4), С. 365 - 371

Опубликована: Дек. 2, 2023

Язык: Английский

Процитировано

25
Roles of chromatin and genome instability in cellular senescence and their relevance to ageing and related diseases DOI
Zeming Wu, Jing Qu, Guang‐Hui Liu

и другие.

Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(12), С. 979 - 1000

Опубликована: Окт. 3, 2024

Язык: Английский

Процитировано

10
Insights and Interventions in Age-Associated Inflammation DOI
Haoyan Huang, Jie Ren, Guang‐Hui Liu

и другие.

Current Opinion in Genetics & Development, Год журнала: 2025, Номер 91, С. 102306 - 102306

Опубликована: Янв. 20, 2025

Язык: Английский

Процитировано

1
The intersection of aging and estrogen in osteoarthritis DOI Creative Commons
Aysegul Atasoy‐Zeybek,

Kelly K. Showel,

Christopher V. Nagelli

и другие.

npj Women s Health, Год журнала: 2025, Номер 3(1)

Опубликована: Фев. 25, 2025

Osteoarthritis (OA) is a chronic joint disease characterized by cartilage degradation, inflammation, and pain. While multiple factors contribute to OA development, age sex are primary risk factors, particularly affecting postmenopausal women. The dramatic increase in after menopause suggests estrogen deficiency accelerates progression. This review explores the molecular mechanisms connecting aging focusing on key genes pathways identified through RNA sequencing.

Язык: Английский

Процитировано

1
Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity DOI Creative Commons
Yihua Lyu, Qiang Fu, Dominika Wilczok

и другие.

Aging, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

Aging | doi:10.18632/aging.206135. Yu-Xuan Lyu, Qiang Fu, Dominika Wilczok, Kejun Ying, Aaron King, Adam Antebi, Aleksandar Vojta, Alexandra Stolzing, Alexey Moskalev, Anastasia Georgievskaya, Andrea B. Maier, Olsen, Anja Groth, Anna Katharina Simon, Anne Brunet, Aisyah Jamil, Anton Kulaga, Asif Bhatti, Benjamin Yaden, Bente Klarlund Pedersen, Björn Schumacher, Boris Djordjevic, Brian Kennedy, Chieh Chen, Christine Yuan Huang, Christoph U. Correll, Coleen T. Murphy, Collin Y. Ewald, Danica Dario Riccardo Valenzano, Dariusz Sołdacki, David Erritzoe, Meyer, A. Sinclair, Eduardo Nunes Chini, Emma C. Teeling, Eric Morgen, Verdin, Erik Vernet, Estefano Pinilla, Evandro F. Fang, Evelyne Bischof, Evi M. Mercken, Fabian Finger, Folkert Kuipers, Frank W. Pun, Gabor Gyülveszi, Gabriele Civiletto, Garri Zmudze, Gil Blander, Harold Pincus, Joshua McClure, James L. Kirkland, Peyer, Jamie N. Justice, Jan Vijg, Jennifer R. Gruhn, Jerry McLaughlin, Joan Mannick, João Passos, Joseph Baur, Joe Betts-LaCroix, John Sedivy, Speakman, Jordan Shlain, Julia von Maltzahn, Katrin I. Andreasson, Kelsey Moody, Konstantinos Palikaras, Kristen Fortney, Laura J. Niedernhofer, Lene Juel Rasmussen, Liesbeth Veenhoff, Lisa Melton, Luigi Ferrucci, Marco Quarta, Maria Koval, Marinova, Mark Hamalainen, Maximilian Unfried, Michael S. Ringel, Milos Filipovic, Mourad Topors, Natalia Mitin, Nawal Roy, Nika Pintar, Nir Barzilai, Paolo Binetti, Parminder Singh, Paul Kohlhaas, D. Robbins, Rubin, Peter O. Fedichev, Petrina Kamya, Pura Muñoz-Canoves, Rafael de Cabo, Richard G. Faragher, Rob Konrad, Roberto Ripa, Robin Mansukhani, Sabrina Büttner, Sara Wickström, Sebastian Brunemeier, Sergey Jakimov, Shan Luo, Sharon Rosenzweig-Lipson, Shih-Yin Tsai, Stefanie Dimmeler, Thomas Rando, Tim Peterson, Tina Woods, Tony Wyss-Coray, Toren Finkel, Tzipora Strauss, Vadim Gladyshev, Valter Longo, Varun Dwaraka, Vera Gorbunova, Victoria Acosta-Rodríguez, Vincenzo Sorrentino, Vittorio Sebastiano, Wenbin Li, Yousin Suh, Alex Zhavoronkov, Morten Scheibye-Knudsen, Daniela Bakula

Язык: Английский

Процитировано

8
Therapeutic strategies targeting cellular senescence for cancer and other diseases DOI Creative Commons
Xuebing Wang, Takeshi Fukumoto, Ken-ichi Noma

и другие.

The Journal of Biochemistry, Год журнала: 2024, Номер 175(5), С. 525 - 537

Опубликована: Фев. 15, 2024

Abstract Cellular senescence occurs in response to endogenous or exogenous stresses and is characterized by stable cell cycle arrest, alterations nuclear morphology secretion of proinflammatory factors, referred as the senescence-associated secretory phenotype (SASP). An increase senescent cells associated with development several types cancer aging-related diseases. Therefore, senolytic agents that selectively remove may offer opportunities for developing new therapeutic strategies against such cancers This review outlines inducers general characteristics cells. We also discuss involvement certain Finally, we describe a series their utilization strategies.

Язык: Английский

Процитировано

6
A panoramic view of cell population dynamics in mammalian aging DOI
Zehao Zhang, Chloe Schaefer,

Weirong Jiang

и другие.

Science, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

To elucidate aging-associated cellular population dynamics, we present PanSci , a single-cell transcriptome atlas profiling over 20 million cells from 623 mouse tissues across different life stages, sexes, and genotypes. This comprehensive dataset reveals more than 3,000 unique states 200 cell populations. Our panoramic analysis uncovered organ-, lineage-, sex-specific shifts of dynamics during lifespan progression. Moreover, identify both systematic organ-specific alterations in immune populations associated with aging. We further explored the regulatory roles system on aging pinpointed specific age-related expansions that are lymphocyte dependent. “cell-omics” strategy enhances comprehension lays groundwork for exploring complex networks diseases.

Язык: Английский

Процитировано

6
Emerging technology has a brilliant future: the CRISPR-Cas system for senescence, inflammation, and cartilage repair in osteoarthritis DOI Creative Commons
Shicheng Jia,

Rongji Liang,

Jiayou Chen

и другие.

Cellular & Molecular Biology Letters, Год журнала: 2024, Номер 29(1)

Опубликована: Май 2, 2024

Abstract Osteoarthritis (OA), known as one of the most common types aseptic inflammation musculoskeletal system, is characterized by chronic pain and whole-joint lesions. With cellular molecular changes including senescence, inflammatory alterations, subsequent cartilage defects, OA eventually leads to a series adverse outcomes such disability. CRISPR-Cas-related technology has been proposed explored gene therapy, offering potential gene-editing tools that are in spotlight. Considering genetic multigene regulatory mechanisms OA, we systematically review current studies on CRISPR-Cas for improving terms inflammation, damage summarize various strategies delivering CRISPR products, hoping provide new perspective treatment taking advantage technology.

Язык: Английский

Процитировано

5