ETV2 Overexpression Promotes Efficient Differentiation of Pluripotent Stem Cells to Endothelial Cells DOI Creative Commons
Yunfeng Ding,

Soniya Tamhankar,

Feifan Du

и другие.

Biotechnology and Bioengineering, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Differentiating endothelial cells (ECs) from human pluripotent stem (hPSCs) typically takes 2 weeks and requires parameter optimization. Overexpression of cell type-specific transcription factors in hPSCs has shown efficient differentiation into various types. ETV2, a crucial factor for fate, can be overexpressed to induce rapid facile EC (iETV2-ECs). We developed two-stage strategy which involves differentiating inducible ETV2-overexpressing basal induction medium during stage I expanding them an II. By optimizing seeding density composition, we achieved 99% pure CD31+ CD144+ iETV2-ECs without sorting 5 days. demonstrated vitro angiogenesis potential, LDL uptake, cytokine response. Transcriptomic comparisons revealed similar gene expression profiles between traditionally differentiated ECs. Additionally, responded Wnt signaling agonist TGFβ inhibitor acquire brain phenotypes, making scalable source applications including blood-brain barrier modeling.

Язык: Английский

Vascularized human brain organoids: current possibilities and prospects DOI Creative Commons
Lois Kistemaker, Emma J. van Bodegraven, Helga E. de Vries

и другие.

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Human brain organoids (hBOs) are in vitro, 3D, self-organizing tissue structures increasingly used for modeling development and disease. Although they traditionally lack vasculature, recent bioengineering developments enable their vascularization, which partly recapitulates neurodevelopmental processes such as neural tube angiogenesis, formation of neurovascular unit (NVU)-like structures, early barriergenesis. vascularized hBOs (vhBOs) already to model (defects in) development, vascularization efficiency other outcomes differ substantially between protocols overall shortcomings should be considered. For instance, vessel-like vhBOs do not contain blood-like flow nor form a functional blood-brain barrier (BBB). Extended characterization, standardization, the new techniques may broader applications drug transport studies.

Язык: Английский

Процитировано

8

High‐Throughput Formation of Pre‐Vascularized hiPSC‐Derived Hepatobiliary Organoids on a Chip via Nonparenchymal Cell Grafting DOI Creative Commons
Han Fan, J. K. Shang, Junbo Li

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 4, 2025

Abstract Liver organoids have been increasingly adopted as a critical in vitro model to study liver development and diseases. However, the pre‐vascularization of without affecting parenchymal specification remains long‐lasting challenge, which is essential for their application regenerative medicine. Here, large‐scale formation pre‐vascularized human hepatobiliary (vhHBOs) presented epithelial via novel strategy, namely nonparenchymal cell grafting (NCG). Endothelial mesenchymal cells are grafted (hHBOs) at different differentiation stages supplementing with culture medium growth factors. stage hepatic maturation offers an optimal integration efficiency compared specification. Additionally, proves crucial endothelial invading sprouting into during establishment vhHBOs. Ectopic implants mice further displayed vhHBOs vascular networks. Notably, transplanted self‐organized native tissue like zone bile ducts, indicating potential regenerate damaged duct tissues. It believed that will offer technical route form high‐fidelity complex engineering

Язык: Английский

Процитировано

3

Vascular network-inspired diffusible scaffolds for engineering functional midbrain organoids DOI
Hongwei Cai,

Chunhui Tian,

Lei Chen

и другие.

Cell stem cell, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

3

Assembloid models of cell-cell interaction to study tissue and disease biology DOI
Massimo M. Onesto, Ji‐il Kim, Sergiu P. Paşca

и другие.

Cell stem cell, Год журнала: 2024, Номер 31(11), С. 1563 - 1573

Опубликована: Окт. 24, 2024

Язык: Английский

Процитировано

13

Bioengineering tools for next-generation neural organoids DOI Creative Commons
Richard O’Laughlin,

F. C. Cheng,

Hongjun Song

и другие.

Current Opinion in Neurobiology, Год журнала: 2025, Номер 92, С. 103011 - 103011

Опубликована: Март 24, 2025

Язык: Английский

Процитировано

1

Biomanufacturing and lipidomics analysis of extracellular vesicles secreted by human blood vessel organoids in a vertical wheel bioreactor DOI Creative Commons
Justice Ene, Chang Liu,

Farhat Syed

и другие.

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 24, 2025

Extracellular vesicles (EVs) derived from human organoids are phospholipid bilayer-bound nanoparticles that carry therapeutic cargo. However, the low yield of EVs remains a critical bottleneck for clinical translation. Vertical-Wheel bioreactors (VWBRs), with unique design features, facilitate scalable production secreted by blood vessel (BVOs) under controlled shear stress, using aggregate- and microcarrier-based culture systems. Human induced pluripotent stem cell-derived BVOs cultured as aggregates or on Synthemax II microcarriers within VWBRs (40 80 rpm) were compared to static controls. The characterized metabolite profiling, flow cytometry, gene expression EV biogenesis markers. nanoparticle tracking analysis, electron microscopy, Western blotting. Lipidomics provided insights into lipid composition, while functional assays assessed impact in D-galactose-induced senescence model. VWBR cultures showed more aerobic metabolism higher genes control. different conditions comparable size, but yields significantly microcarrier dynamic than aggregates. Lipidomic profiling revealed minimal variation (< 0.36%) total content; however, distinct differences identified chain lengths saturation levels, affecting key pathways such sphingolipid neurotrophin signaling. BVO demonstrated abilities reducing oxidative stress increasing cell proliferation vitro. differentiated (in particular 40 produce 2-3 fold (per mL) bio manufactured have exosomal characteristics cargo, showing properties vitro assays. This innovative approach establishes platform producing defined profiles potential, paving way future vivo studies.

Язык: Английский

Процитировано

1

Organoids: development and applications in disease models, drug discovery, precision medicine, and regenerative medicine DOI Creative Commons
Qigu Yao, Sheng Cheng, Qiaoling Pan

и другие.

MedComm, Год журнала: 2024, Номер 5(10)

Опубликована: Сен. 21, 2024

Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions specific organs. Although field organoids has experienced exponential growth, driven by advances in artificial intelligence, gene editing, bioinstrumentation, a comprehensive overview organoid applications remains necessary. This review offers detailed exploration historical origins characteristics various types, their applications-including disease modeling, drug toxicity efficacy assessments, precision medicine, regenerative medicine-as well as current challenges future directions research. have proven instrumental elucidating genetic cell fate hereditary diseases, infectious metabolic disorders, malignancies, study processes such embryonic development, molecular mechanisms, host-microbe interactions. Furthermore, integration technology with intelligence microfluidics significantly advanced large-scale, rapid, cost-effective thereby propelling progress medicine. Finally, advent high-performance materials, three-dimensional printing technology, also gaining prominence Our insights predictions aim to provide valuable guidance researchers support continued advancement this rapidly developing field.

Язык: Английский

Процитировано

9

Brain organoids: building higher-order complexity and neural circuitry models DOI Creative Commons

Gulimiheranmu Maisumu,

Stephanie M. Willerth, Michael W. Nestor

и другие.

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1

Novel human iPSC models of neuroinflammation in neurodegenerative disease and regenerative medicine DOI Creative Commons

Rose Ana Summers,

Francesca Fagiani, David H. Rowitch

и другие.

Trends in Immunology, Год журнала: 2024, Номер 45(10), С. 799 - 813

Опубликована: Сен. 21, 2024

Язык: Английский

Процитировано

5

Human midbrain organoids: a powerful tool for advanced Parkinson’s disease modeling and therapy exploration DOI Creative Commons
Xin Cui, Xinwei Li, Huimin Zheng

и другие.

npj Parkinson s Disease, Год журнала: 2024, Номер 10(1)

Опубликована: Окт. 20, 2024

Parkinson's disease (PD) is a neurodegenerative disorder marked by the loss of dopaminergic neurons in substantia nigra. Despite progress, pathogenesis remains unclear. Human midbrain organoids (hMLOs) have emerged as promising model for studying PD, drug screening, and potential treatments. This review discusses development hMLOs, their application PD research, current challenges organoid construction, highlighting possible optimization strategies.

Язык: Английский

Процитировано

5