Cell Reports, Год журнала: 2025, Номер 44(5), С. 115576 - 115576
Опубликована: Апрель 14, 2025
Язык: Английский
Trends in biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Human brain organoids (hBOs) are in vitro, 3D, self-organizing tissue structures increasingly used for modeling development and disease. Although they traditionally lack vasculature, recent bioengineering developments enable their vascularization, which partly recapitulates neurodevelopmental processes such as neural tube angiogenesis, formation of neurovascular unit (NVU)-like structures, early barriergenesis. vascularized hBOs (vhBOs) already to model (defects in) development, vascularization efficiency other outcomes differ substantially between protocols overall shortcomings should be considered. For instance, vessel-like vhBOs do not contain blood-like flow nor form a functional blood-brain barrier (BBB). Extended characterization, standardization, the new techniques may broader applications drug transport studies.
Язык: Английский
Процитировано
8
Cell stem cell, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
5
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 4, 2025
Abstract Liver organoids have been increasingly adopted as a critical in vitro model to study liver development and diseases. However, the pre‐vascularization of without affecting parenchymal specification remains long‐lasting challenge, which is essential for their application regenerative medicine. Here, large‐scale formation pre‐vascularized human hepatobiliary (vhHBOs) presented epithelial via novel strategy, namely nonparenchymal cell grafting (NCG). Endothelial mesenchymal cells are grafted (hHBOs) at different differentiation stages supplementing with culture medium growth factors. stage hepatic maturation offers an optimal integration efficiency compared specification. Additionally, proves crucial endothelial invading sprouting into during establishment vhHBOs. Ectopic implants mice further displayed vhHBOs vascular networks. Notably, transplanted self‐organized native tissue like zone bile ducts, indicating potential regenerate damaged duct tissues. It believed that will offer technical route form high‐fidelity complex engineering
Язык: Английский
Процитировано
3
Cell stem cell, Год журнала: 2024, Номер 31(11), С. 1563 - 1573
Опубликована: Окт. 24, 2024
Язык: Английский
Процитировано
14
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Апрель 24, 2025
Extracellular vesicles (EVs) derived from human organoids are phospholipid bilayer-bound nanoparticles that carry therapeutic cargo. However, the low yield of EVs remains a critical bottleneck for clinical translation. Vertical-Wheel bioreactors (VWBRs), with unique design features, facilitate scalable production secreted by blood vessel (BVOs) under controlled shear stress, using aggregate- and microcarrier-based culture systems. Human induced pluripotent stem cell-derived BVOs cultured as aggregates or on Synthemax II microcarriers within VWBRs (40 80 rpm) were compared to static controls. The characterized metabolite profiling, flow cytometry, gene expression EV biogenesis markers. nanoparticle tracking analysis, electron microscopy, Western blotting. Lipidomics provided insights into lipid composition, while functional assays assessed impact in D-galactose-induced senescence model. VWBR cultures showed more aerobic metabolism higher genes control. different conditions comparable size, but yields significantly microcarrier dynamic than aggregates. Lipidomic profiling revealed minimal variation (< 0.36%) total content; however, distinct differences identified chain lengths saturation levels, affecting key pathways such sphingolipid neurotrophin signaling. BVO demonstrated abilities reducing oxidative stress increasing cell proliferation vitro. differentiated (in particular 40 produce 2-3 fold (per mL) bio manufactured have exosomal characteristics cargo, showing properties vitro assays. This innovative approach establishes platform producing defined profiles potential, paving way future vivo studies.
Язык: Английский
Процитировано
2
MedComm, Год журнала: 2024, Номер 5(10)
Опубликована: Сен. 21, 2024
Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions specific organs. Although field organoids has experienced exponential growth, driven by advances in artificial intelligence, gene editing, bioinstrumentation, a comprehensive overview organoid applications remains necessary. This review offers detailed exploration historical origins characteristics various types, their applications-including disease modeling, drug toxicity efficacy assessments, precision medicine, regenerative medicine-as well as current challenges future directions research. have proven instrumental elucidating genetic cell fate hereditary diseases, infectious metabolic disorders, malignancies, study processes such embryonic development, molecular mechanisms, host-microbe interactions. Furthermore, integration technology with intelligence microfluidics significantly advanced large-scale, rapid, cost-effective thereby propelling progress medicine. Finally, advent high-performance materials, three-dimensional printing technology, also gaining prominence Our insights predictions aim to provide valuable guidance researchers support continued advancement this rapidly developing field.
Язык: Английский
Процитировано
9
Current Opinion in Neurobiology, Год журнала: 2025, Номер 92, С. 103011 - 103011
Опубликована: Март 24, 2025
Язык: Английский
Процитировано
1
Trends in biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
1
npj Parkinson s Disease, Год журнала: 2024, Номер 10(1)
Опубликована: Окт. 20, 2024
Parkinson's disease (PD) is a neurodegenerative disorder marked by the loss of dopaminergic neurons in substantia nigra. Despite progress, pathogenesis remains unclear. Human midbrain organoids (hMLOs) have emerged as promising model for studying PD, drug screening, and potential treatments. This review discusses development hMLOs, their application PD research, current challenges organoid construction, highlighting possible optimization strategies.
Язык: Английский
Процитировано
6
Breast Cancer Research, Год журнала: 2024, Номер 26(1)
Опубликована: Июль 1, 2024
Abstract Background Metastasis, the spread, and growth of malignant cells at secondary sites within a patient’s body, accounts for over 90% cancer-related mortality. Breast cancer is most common tumor type diagnosed leading cause lethality in women United States. It estimated that 10–16% breast patients will have brain metastasis. Current therapies to treat with metastasis (BCBM) remain palliative. This largely due our limited understanding fundamental molecular cellular mechanisms through which BCBM progresses, represents critical barrier development efficient affected patients. Methods Previous research relied on co-culture assays rodent neural or slice ex vivo. Given need overcome obstacle human-relevant host study cell-cell communication BCBM, we generated human embryonic stem cell-derived cerebral organoids cell lines. We used MDA-MB-231 its metastatic derivate Br-EGFP, other lines MCF-7, HCC-1806, SUM159PT. leveraged this novel 3D platform investigate crosstalk organoid. Results found SUM159PT formed colonies organoids. Moreover, Br-EGFP showed increased capacity invade expand Conclusions Our model has demonstrated remarkable discern ability The generation BCBM-like structures organoid facilitate microenvironment culture.
Язык: Английский
Процитировано
5