Biotechnology and Bioengineering,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
Differentiating
endothelial
cells
(ECs)
from
human
pluripotent
stem
(hPSCs)
typically
takes
2
weeks
and
requires
parameter
optimization.
Overexpression
of
cell
type-specific
transcription
factors
in
hPSCs
has
shown
efficient
differentiation
into
various
types.
ETV2,
a
crucial
factor
for
fate,
can
be
overexpressed
to
induce
rapid
facile
EC
(iETV2-ECs).
We
developed
two-stage
strategy
which
involves
differentiating
inducible
ETV2-overexpressing
basal
induction
medium
during
stage
I
expanding
them
an
II.
By
optimizing
seeding
density
composition,
we
achieved
99%
pure
CD31+
CD144+
iETV2-ECs
without
sorting
5
days.
demonstrated
vitro
angiogenesis
potential,
LDL
uptake,
cytokine
response.
Transcriptomic
comparisons
revealed
similar
gene
expression
profiles
between
traditionally
differentiated
ECs.
Additionally,
responded
Wnt
signaling
agonist
TGFβ
inhibitor
acquire
brain
phenotypes,
making
scalable
source
applications
including
blood-brain
barrier
modeling.
Trends in biotechnology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Human
brain
organoids
(hBOs)
are
in
vitro,
3D,
self-organizing
tissue
structures
increasingly
used
for
modeling
development
and
disease.
Although
they
traditionally
lack
vasculature,
recent
bioengineering
developments
enable
their
vascularization,
which
partly
recapitulates
neurodevelopmental
processes
such
as
neural
tube
angiogenesis,
formation
of
neurovascular
unit
(NVU)-like
structures,
early
barriergenesis.
vascularized
hBOs
(vhBOs)
already
to
model
(defects
in)
development,
vascularization
efficiency
other
outcomes
differ
substantially
between
protocols
overall
shortcomings
should
be
considered.
For
instance,
vessel-like
vhBOs
do
not
contain
blood-like
flow
nor
form
a
functional
blood-brain
barrier
(BBB).
Extended
characterization,
standardization,
the
new
techniques
may
broader
applications
drug
transport
studies.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 4, 2025
Abstract
Liver
organoids
have
been
increasingly
adopted
as
a
critical
in
vitro
model
to
study
liver
development
and
diseases.
However,
the
pre‐vascularization
of
without
affecting
parenchymal
specification
remains
long‐lasting
challenge,
which
is
essential
for
their
application
regenerative
medicine.
Here,
large‐scale
formation
pre‐vascularized
human
hepatobiliary
(vhHBOs)
presented
epithelial
via
novel
strategy,
namely
nonparenchymal
cell
grafting
(NCG).
Endothelial
mesenchymal
cells
are
grafted
(hHBOs)
at
different
differentiation
stages
supplementing
with
culture
medium
growth
factors.
stage
hepatic
maturation
offers
an
optimal
integration
efficiency
compared
specification.
Additionally,
proves
crucial
endothelial
invading
sprouting
into
during
establishment
vhHBOs.
Ectopic
implants
mice
further
displayed
vhHBOs
vascular
networks.
Notably,
transplanted
self‐organized
native
tissue
like
zone
bile
ducts,
indicating
potential
regenerate
damaged
duct
tissues.
It
believed
that
will
offer
technical
route
form
high‐fidelity
complex
engineering
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Апрель 24, 2025
Extracellular
vesicles
(EVs)
derived
from
human
organoids
are
phospholipid
bilayer-bound
nanoparticles
that
carry
therapeutic
cargo.
However,
the
low
yield
of
EVs
remains
a
critical
bottleneck
for
clinical
translation.
Vertical-Wheel
bioreactors
(VWBRs),
with
unique
design
features,
facilitate
scalable
production
secreted
by
blood
vessel
(BVOs)
under
controlled
shear
stress,
using
aggregate-
and
microcarrier-based
culture
systems.
Human
induced
pluripotent
stem
cell-derived
BVOs
cultured
as
aggregates
or
on
Synthemax
II
microcarriers
within
VWBRs
(40
80
rpm)
were
compared
to
static
controls.
The
characterized
metabolite
profiling,
flow
cytometry,
gene
expression
EV
biogenesis
markers.
nanoparticle
tracking
analysis,
electron
microscopy,
Western
blotting.
Lipidomics
provided
insights
into
lipid
composition,
while
functional
assays
assessed
impact
in
D-galactose-induced
senescence
model.
VWBR
cultures
showed
more
aerobic
metabolism
higher
genes
control.
different
conditions
comparable
size,
but
yields
significantly
microcarrier
dynamic
than
aggregates.
Lipidomic
profiling
revealed
minimal
variation
(<
0.36%)
total
content;
however,
distinct
differences
identified
chain
lengths
saturation
levels,
affecting
key
pathways
such
sphingolipid
neurotrophin
signaling.
BVO
demonstrated
abilities
reducing
oxidative
stress
increasing
cell
proliferation
vitro.
differentiated
(in
particular
40
produce
2-3
fold
(per
mL)
bio
manufactured
have
exosomal
characteristics
cargo,
showing
properties
vitro
assays.
This
innovative
approach
establishes
platform
producing
defined
profiles
potential,
paving
way
future
vivo
studies.
Organoids
are
miniature,
highly
accurate
representations
of
organs
that
capture
the
structure
and
unique
functions
specific
organs.
Although
field
organoids
has
experienced
exponential
growth,
driven
by
advances
in
artificial
intelligence,
gene
editing,
bioinstrumentation,
a
comprehensive
overview
organoid
applications
remains
necessary.
This
review
offers
detailed
exploration
historical
origins
characteristics
various
types,
their
applications-including
disease
modeling,
drug
toxicity
efficacy
assessments,
precision
medicine,
regenerative
medicine-as
well
as
current
challenges
future
directions
research.
have
proven
instrumental
elucidating
genetic
cell
fate
hereditary
diseases,
infectious
metabolic
disorders,
malignancies,
study
processes
such
embryonic
development,
molecular
mechanisms,
host-microbe
interactions.
Furthermore,
integration
technology
with
intelligence
microfluidics
significantly
advanced
large-scale,
rapid,
cost-effective
thereby
propelling
progress
medicine.
Finally,
advent
high-performance
materials,
three-dimensional
printing
technology,
also
gaining
prominence
Our
insights
predictions
aim
to
provide
valuable
guidance
researchers
support
continued
advancement
this
rapidly
developing
field.
npj Parkinson s Disease,
Год журнала:
2024,
Номер
10(1)
Опубликована: Окт. 20, 2024
Parkinson's
disease
(PD)
is
a
neurodegenerative
disorder
marked
by
the
loss
of
dopaminergic
neurons
in
substantia
nigra.
Despite
progress,
pathogenesis
remains
unclear.
Human
midbrain
organoids
(hMLOs)
have
emerged
as
promising
model
for
studying
PD,
drug
screening,
and
potential
treatments.
This
review
discusses
development
hMLOs,
their
application
PD
research,
current
challenges
organoid
construction,
highlighting
possible
optimization
strategies.
