The
composition
of
glycerolipids
from
a
range
ocean
environments
were
analyzed
to
better
understand
the
biochemical
adaptations
and
remineralization
processes
planktonic
biomass.
Glycerolipids
compose
cell
membranes
energy
stores
microorganisms.
As
group
they
present
useful
target
for
tracking
wide
environmental
stress
responses.
In
Chapter
2
this
work,
I
employ
high
resolution
mass
spectrometry
assemble
global
dataset
lipidomes.
Using
samples
mostly
epipelagic,
find
that
water
temperature
is
major
controlling
factor
on
fatty
acid
saturation
state
hypothesize
derives
known
biophysical
constraints
membranes.
extend
analysis
further
investigate
links
between
long-chain
essential
acids
(LCEFA)
temperature.
My
shows
abundance
one
these
LCEFAs
(eicosapentaenoic
acid,
20:5
n-3)
well
correlated
with
relationship,
project
temperature-based
declines
in
its
due
climate
change
over
next
century.
3,
using
West
Antarctic
peninsula,
assess
pigments
glycerolipid
compositions
microbial
communities
within
seasonal
pack
ice
floes
gain
insights
growth
an
extremophile
environment.
caloric
content
biomass
additionally
calculated
value
higher
trophic
level
consumers
sea
ecosystem.
glycerolipids’
physical
distribution
core
be
calorically
dense
but
highly
geographically
physically
heterogenous.
show
evidence
new
biomarker
(fatty
hydroxy
triacylglycerols,
FAHFA-TAG)
which
have
not
been
detected
before
system.
evaluate
possible
sources
novel
biomarkers
ice.
Lastly,
examine
sinking
material
two
separate
studies
carbon
flux
upper
mesopelagic.
These
revealed
both
influencing
lipid
carbon.
lost
non-selectively
particles,
differing
previous
reports
selective
degradation
lipids.
However,
compositional
differences
surface-suspended
possibly
pointing
selection
mechanisms
at
conception.
use
create
source
metric
predicting
relative
amounts
derived
fecal
pellets
verse
aggregates.
findings
thesis
our
understanding
their
environment
reveal
factors
cycling
Advanced Materials,
Год журнала:
2024,
Номер
36(23)
Опубликована: Март 8, 2024
Abstract
The
sheet‐like
lipid
bilayer
is
the
fundamental
structural
component
of
all
cell
membranes.
Its
building
blocks
are
phospholipids
and
cholesterol.
Their
amphiphilic
structure
spontaneously
leads
to
formation
a
in
aqueous
environment.
Lipids
not
just
elements.
Individual
species,
membrane
structure,
dynamics
influence
regulate
protein
function.
An
exciting
field
emerging
where
membrane‐associated
material
properties
different
systems
used
designing
innovative
solutions
for
widespread
applications
across
various
fields,
such
as
food
industry,
cosmetics,
nano‐
biomedicine,
drug
storage
delivery,
biotechnology,
biosensors,
computing.
Here,
authors
summarize
what
known
about
how
lipids
determine
functions
biological
membranes
this
has
been
or
can
be
translated
into
applications.
Based
on
recent
progress
understanding
dynamics,
physical
properties,
perspective
provided
membrane‐controlled
regulation
extend
current
even
offer
new
The EMBO Journal,
Год журнала:
2024,
Номер
43(8), С. 1653 - 1685
Опубликована: Март 15, 2024
Abstract
Biological
membranes
have
a
stunning
ability
to
adapt
their
composition
in
response
physiological
stress
and
metabolic
challenges.
Little
is
known
how
such
perturbations
affect
individual
organelles
eukaryotic
cells.
Pioneering
work
has
provided
insights
into
the
subcellular
distribution
of
lipids
yeast
Saccharomyces
cerevisiae
,
but
endoplasmic
reticulum
(ER)
membrane,
which
also
crucially
regulates
lipid
metabolism
unfolded
protein
response,
remains
insufficiently
characterized.
Here,
we
describe
method
for
purifying
organelle
from
yeast,
MemPrep.
We
demonstrate
purity
our
ER
membrane
preparations
by
proteomics,
document
general
utility
MemPrep
isolating
vacuolar
membranes.
Quantitative
lipidomics
establishes
membrane.
Our
findings
provide
baseline
studying
biogenesis
important
implications
understanding
role
regulating
(UPR).
The
combined
preparative
analytical
approach
uncovers
dynamic
remodeling
stressed
cells
distinct
molecular
fingerprints
bilayer
stress.
Foods,
Год журнала:
2025,
Номер
14(3), С. 363 - 363
Опубликована: Янв. 23, 2025
Aquatic
products
are
an
important
part
of
the
human
diet,
but
they
easily
contaminated
by
Pseudomonas
spp.,
which
leads
to
food
deterioration
and
economic
loss.
In
this
paper,
main
characteristics
psychrotrophic
in
aquatic
reviewed,
including
its
growth
adaptation
mechanism
biofilm
formation
ability
at
low
temperatures,
key
role
product
spoilage
is
emphasized.
Studies
have
shown
that
can
produce
a
variety
volatile
compounds
decomposing
proteins
amino
acids,
affecting
sensory
quality
safety
products.
A
control
strategies
extend
shelf
life
been
explored,
physical,
chemical,
biological
methods,
particularly
biofilm-specific
inhibition
techniques
such
as
quorum
sensing
application
natural
antimicrobials.
Future
research
should
prioritize
development
novel
anti-biofilm
address
growing
problem
contamination
industry
ensure
public
health.
ACS Chemical Biology,
Год журнала:
2024,
Номер
19(8), С. 1773 - 1785
Опубликована: Июль 29, 2024
Organelles
feature
characteristic
lipid
compositions
that
lead
to
differences
in
membrane
properties.
In
cells,
ordering
and
fluidity
are
commonly
measured
using
the
solvatochromic
dye
Laurdan,
whose
fluorescence
is
sensitive
packing.
As
a
general
lipophilic
dye,
Laurdan
stains
all
hydrophobic
environments
cells;
therefore,
it
challenging
characterize
properties
specific
organelles
or
assess
their
responses
pharmacological
treatments
intact
cells.
Here,
we
describe
synthesis
application
of
Laurdan-derived
probes
read
out
packing
individual
cellular
organelles.
The
set
organelle-targeted
Laurdans
(OTL)
localizes
ER,
mitochondria,
lysosomes,
Golgi
compartments
with
high
specificity
while
retaining
spectral
resolution
needed
detect
biological
changes
ordering.
We
show
ratiometric
imaging
OTLs
can
resolve
heterogeneity
within
as
well
resulting
from
inhibition
trafficking
bioenergetic
processes.
apply
these
organelle-specific
saturated
stress.
While
ER
lysosomal
exogenous
fatty
acids,
mitochondrial
membranes
protected.
then
use
sort
populations
cells
based
on
acid
diet,
highlighting
ability
organelle-localized
distinguish
between
metabolic
state.
These
results
expand
repertoire
targeted
demonstrate
interrogating
dysregulation.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2024,
Номер
16(8), С. a041400 - a041400
Опубликована: Янв. 22, 2024
Robert
Ernst1,2,
Mike
F.
Renne1,2,
Aamna
Jain1,2
and
Alexander
von
der
Malsburg1,2
1Medical
Biochemistry
Molecular
Biology,
Medical
Faculty,
Saarland
University,
66421
Homburg,
Germany
2Preclinical
Center
for
Signaling
(PZMS),
Correspondence:
robert.ernst{at}uks.eu
