Molecular Therapy — Nucleic Acids,
Год журнала:
2023,
Номер
34, С. 102073 - 102073
Опубликована: Ноя. 10, 2023
The
molecular
weight
of
nucleic
acid
aptamers
(20
kDa)
is
lower
than
the
cutoff
threshold
renal
filtration
(30-50
kDa),
resulting
in
a
very
short
half-life,
which
dramatically
limits
their
druggability.
To
address
this,
we
utilized
3-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-(4-hydroxy-2-oxo-2H-chromen-6-yl)propenamide
(HC)
and
12-((2,5-dioxopyrrolidin-1-yl)oxy)-12-oxododecanoic
(DA),
two
newly
designed
coupling
agents,
for
synergistic
binding
to
human
serum
albumin
(HSA).
Both
HC
DA
are
conjugated
bone
anabolic
aptamer
(Apc001)
against
sclerostin
form
an
Apc001OC
conjugate
with
high
affinity
HSA.
Notably,
could
synergistically
facilitate
prolonging
half-life
Apc001
promoting
its
potential.
Using
blocking
peptides,
mechanism
studies
indicate
that
effect
HC-DA
on
pharmacokinetics
potential
achieved
via
Moreover,
biweekly
at
50
mg/kg
shows
comparable
marketed
antibody
given
weekly
25
mg/kg.
This
proposed
bimolecular
modification
strategy
help
druggability
challenge
half-life.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Ensuring
food
safety
is
a
shared
responsibility
across
the
entire
supply
chain,
encompassing
manufacturers,
processors,
retailers,
consumers,
and
regulatory
bodies.
However,
traditional
detection
methods
have
several
limitations,
including
slow
processing
times,
high
costs,
limited
sensitivity,
susceptibility
to
false
positives
or
negatives.
These
shortcomings
underscore
urgent
need
for
faster,
more
accurate,
cost-effective
technologies.
Aptamers
aptasensors
emerged
as
promising
alternatives.
offer
advantages
over
recognition
probes
due
their
affinity
specificity
diverse
targets.
The
enable
rapid
detection,
cost
reduction,
shelf
life
extension,
minimal
batch-to-batch
variability,
making
them
highly
suitable
applications.
Detecting
small
molecules
such
toxins,
antibiotics,
pesticides,
contaminants,
heavy
metals
remains
challenging
steric
hindrance,
nonspecific
binding,
reduced
accuracy.
Recent
advancements
in
aptamer
technology
focused
on
pre-
postmodifications
enhance
performance.
One
of
most
innovations
development
split
aptamers.
engineered
aptamers,
designed
operate
segments
known
improved
flexibility
binding
specificity,
effectively
addressing
challenges
detecting
small-sized
This
review
examines
evolution
aptamers
aptasensors,
focusing
application
that
are
essential
safety.
It
reported
strategies
modifying
optimizing
selected
providing
details
developing
approach
address
unique
small-molecule
detection.
Additionally,
recent
its
integration
into
aptasensor
highlighted,
showcasing
how
these
revolutionizing
hazards
by
overcoming
limitations
methods.
Journal of Biological Chemistry,
Год журнала:
2023,
Номер
299(10), С. 105225 - 105225
Опубликована: Сен. 9, 2023
Recent
advancements
in
small
RNA
sequencing
have
unveiled
a
previously
hidden
world
of
regulatory
noncoding
RNAs
(sncRNAs)
that
extend
beyond
the
well-studied
interfering
RNAs,
microRNAs,
and
piwi-interacting
RNAs.
This
exploration,
starting
with
tRNA-derived
has
led
to
discovery
diverse
universe
sncRNAs
derived
from
various
longer
structured
such
as
rRNAs,
nucleolar
nuclear
Y
vault
exciting
uncharted
functional
possibilities.
In
this
perspective,
we
discuss
emerging
principles
well-known
RNAi-like
mechanisms,
focusing
on
those
operate
independent
linear
sequence
complementarity
but
rather
function
an
aptamer-like
fashion.
Aptamers
use
3D
structure
for
specific
interactions
ligands
are
modulated
by
modifications
subcellular
environments.
Given
sncRNA
functions
widespread
present
species
lacking
RNAi,
they
may
represent
ancient
principle
predates
RNAi.
We
propose
rethinking
origin
RNAi
its
relationship
these
how
complementary
mechanisms
shape
biological
processes.
Lastly,
highlights
need
caution
using
mimics
research
therapeutics,
their
specificity
is
not
restricted
solely
sequence.
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(10), С. 7052 - 7062
Опубликована: Март 1, 2024
Functional
DNAs
are
valuable
molecular
tools
in
chemical
biology
and
analytical
chemistry
but
suffer
from
low
activities
due
to
their
limited
functionalities.
Here,
we
present
a
chemoenzymatic
method
for
site-specific
installation
of
diverse
functional
groups
on
DNA,
showcase
the
application
this
enhance
catalytic
activity
DNA
catalyst.
Through
introduction
distinct
groups,
such
as
hydroxyl,
carboxyl,
benzyl,
at
specific
positions,
achieve
significant
enhancements
RNA-cleaving
deoxyribozyme
10–23.
A
single
carboxyl
modification
results
100-fold
increase,
while
dual
modifications
(carboxyl
benzyl)
yield
an
approximately
700-fold
increase
when
RNA
cleavage
reaction
is
catalyzed
DNA–RNA
chimeric
substrate.
The
resulting
dually
modified
catalyst,
CaBn,
exhibits
kobs
3.76
min–1
presence
1
mM
Mg2+
can
be
employed
fluorescent
imaging
intracellular
magnesium
ions.
Molecular
dynamics
simulations
reveal
superior
capability
CaBn
recruit
ions
metal-ion-binding
site
2
adopt
catalytically
competent
conformation.
Our
work
provides
broadly
accessible
strategy
functionalization
with
modifications,
offers
highly
active
catalyst
immense
potential
biotechnology.
Biosensors,
Год журнала:
2024,
Номер
14(7), С. 350 - 350
Опубликована: Июль 18, 2024
Aptamers
are
nucleic
acid
sequences
that
specifically
bind
with
target
molecules
and
vital
to
applications
such
as
biosensing,
drug
development,
disease
diagnostics,
etc.
The
traditional
selection
procedure
of
aptamers
is
based
on
the
Systematic
Evolution
Ligands
by
an
Exponential
Enrichment
(SELEX)
process,
which
relies
repeating
cycles
screening
amplification.
With
rapid
development
aptamer
applications,
RNA
XNA
draw
more
attention
than
before.
But
their
troublesome
due
necessary
reverse
transcription
process
(RNA)
or
low
efficiency
accuracy
enzymes
for
amplification
(XNA).
In
light
this,
we
review
recent
advances
in
methods
give
outlook
future
a
non-SELEX
approach,
simplifies
reduces
experimental
costs.
We
first
provide
overview
SELEX
mostly
designed
DNA
introduce
common
tools
methods.
Then
section
current
prepared
demonstrate
efforts
put
into
these
difficulties.
further
predict
trend
lies
do
not
require
divide
immobilized
format
non-immobilized
discuss
how
high-resolution
partitioning
could
facilitate
improvement
accuracy.
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 17, 2024
Cancer-derived
exosomes
carry
a
large
number
of
specific
molecular
profiles
from
cancer
cells
and
have
emerged
as
ideal
biomarkers
for
early
diagnosis.
Accurate
detection
ultralow-abundance
in
complex
biological
samples
remains
great
challenge.
Herein,
novel
SERS
aptasensor
powered
by
cascaded
signal
amplification
CRISPR/Cas13a
Chemical Communications,
Год журнала:
2024,
Номер
60(23), С. 3118 - 3128
Опубликована: Янв. 1, 2024
The
past
few
years
have
seen
expansion
of
organometallic
oligonucleotide
modification
beyond
the
well-established
ferrocene
redox
labels.
New
functionalities
include
improved
base
discrimination
and
luminescent
properties
ribonuclease
activity.
