Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science

Current Protocols, Год журнала: 2023, Номер 3(8)

Опубликована: Авг. 1, 2023

The use of Drosophila melanogaster for studies toxicology has grown considerably in the last decade. model long been appreciated as a versatile and powerful developmental biology genetics because its ease handling, short life cycle, low cost maintenance, molecular genetic accessibility, availability wide range publicly available strains data resources. These features, together with recent unique developments genomics metabolomics, make fly especially relevant timely development new approach methodologies movements toward precision toxicology. Here, we offer perspective on how flies can be leveraged to identify risk factors environmental exposures human health. First, review discuss fundamental toxicologic principles experimental design Drosophila. Next, describe quantitative systems approaches resolve architecture candidate pathways controlling susceptibility toxicants. Finally, summarize current state future promise emerging field metabolomics elaborating toxic mechanisms. © 2023 Authors. Current Protocols published by Wiley Periodicals LLC.

Язык: Английский

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Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Март 19, 2022

Abstract With the increase of human activities, cadmium (Cd) pollution has become a global environmental problem affecting biological metabolism in ecosystem. Cd very long half-life humans and is excreted slowly organs, which poses serious threat to health. In order better understand toxicity effects cadmium, third instar larvae Drosophila melanogaster (Canton-S strain) were exposed different concentrations (1.125 mg/kg, 2.25 4.5 9 mg/kg) cadmium. Trypan blue staining showed that intestinal cell damage increased comet assay indicated significantly more DNA high concentrations. The nitroblue tetrazolium (NBT) experiments proved content reactive oxygen species (ROS) increased, exposure could induce oxidative stress. addition, expression mitochondrial adenine nucleotide transferase coding gene ( sesB Ant2 ) apoptosis related genes Debcl, hid, rpr, p53, Sce Diap1 changed, may lead apoptosis. These findings confirmed on stress after early exposure, providing insights into understanding heavy metal animal development.

Язык: Английский

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Effects of heavy metal accumulation mediated by floral rewards on key stages of growth and development of bumblebees (Bombus terrestris L.)

Environmental Toxicology and Chemistry, Год журнала: 2025, Номер 44(1), С. 294 - 301

Опубликована: Янв. 1, 2025

Whether soil heavy metal pollution contributes to the decline in pollinator populations remains inconclusive. Based on detection of metals nectar, pollen, and bodies pollinating insects areas affected by pollution, this study aimed investigate impact adding four metals-zinc (Zn), lead (Pb), copper (Cu), nickel (Ni)-at realistic concentrations diet pre-oviposition period, larval development production adult bees, weight worker bees. The sought evaluate influence accumulation mediated floral rewards key stages growth bumblebee (Bombus terrestris L.). Results revealed that Zn, Pb, Cu significantly extended period queen with Pb doubling time at high Ni showing no significant effect moderate low concentrations. Lead, Cu, medium concentrations, whereas Zn only had a reduced bee yield body bees different among which 25%-40%. concentration was most obvious, reduction 30%. Our findings support hypothesis rewards, can have serious some bumblebees.

Язык: Английский

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Transgenerational Reproductive and Developmental Toxicity Induced by N‐Nitrosodimethylamine and Its Metabolite Formaldehyde in Drosophila melanogaster

Journal of Applied Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

ABSTRACT N ‐Nitrosodimethylamine (NDMA) is a known water disinfection byproduct (DBP) characterized as potent hepatotoxin, promutagen, and probable human carcinogen; this because of the metabolites associated with its biotransformation. The metabolism NDMA produces formaldehyde, another alkylating agent DBP. Both compounds are generated from natural anthropogenic sources, but safety restrictions applied to do not extend uses formaldehyde. Hence, potential health ecological risks concern. Due limited information on long‐term effects exposure these at environmentally relevant concentrations, work aimed compare transgenerational reproductive developmental toxicity separate exposures or metabolite formaldehyde in Drosophila melanogaster over four generations. parental flies were fed (1.19E−06 5 mM) for 48 h during third larval instar. Subsequent offspring (F1–F3) grown under compound‐free conditions. In generation, both modified time emergence reduced number progenies. NDMA, was lethal, affected fertility, weakly induced malformations. next generations, malformed offspring. Reproductive malformations maintained least three suggesting that detrimental could unexposed This first study reporting individual reproduction development between D. , highlighting relevance evaluating multiple generations accurately determine environmental pollutants.

Язык: Английский

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Transgenerational hormesis: What do parents sacrifice for their offspring?

Current Opinion in Environmental Science & Health, Год журнала: 2022, Номер 29, С. 100380 - 100380

Опубликована: Июль 15, 2022

Язык: Английский

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Cadmium chloride exposure impairs the growth and behavior of Drosophila via ferroptosis

The Science of The Total Environment, Год журнала: 2022, Номер 865, С. 161183 - 161183

Опубликована: Дек. 27, 2022

Язык: Английский

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Transgenerational inheritance of wing development defects in Drosophila melanogaster induced by cadmium

Ecotoxicology and Environmental Safety, Год журнала: 2022, Номер 250, С. 114486 - 114486

Опубликована: Дек. 30, 2022

The transgenerational inheritance of phenotype induced by environmental factors is a new focus in epigenetic research. In this study, Drosophila melanogaster (F0) was cultured the medium containing cadmium (Cd, 4.5 mg/kg) from eggs to adults, and offspring (F1-F4) were continuously kept standard (without cadmium). analysis showed that developmental defects on wings apoptosis wing disc cells (F0). transmitted for at least four generations even without Cd afterwards. And effect mRNA expression development related genes (shg, omb, F-actin, Mekk1) can be maintained two or three generations. More importantly, under stress, post-translational modification (PTM) histones H3K4me3 third instar larvae ovaries testes adult flies increased significantly, while levels H3K9me3 H3K27me3 decreased significantly. histone methylation (dSet-1, ash1, Lsd1) significantly these changes one testes. These results suggest phenotypic caused inherited offspring, may regulation methylation. Therefore, adaptability should considered when evaluating toxicity risk cadmium.

Язык: Английский

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Cadmium Transforms Astrocytes into the A1 Subtype via Inducing Gap Junction Protein Connexin 43 into the Nucleus

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(31), С. 12043 - 12051

Опубликована: Июль 20, 2023

Cadmium is highly toxic and present in the environment can be accumulated among various levels of food chain. Both humans animals are at risk from toxicity associated with cadmium. However, neurological endpoint caused by cadmium has not been revealed. The aim our research to explore potential target attack when causing neurotoxicity. 80 male chickens (one day old, weighing 36.49 ± 2.88 g) were randomly divided into four groups independently treated 0, 35, 70, or 140 mg/kg CdCl2 diet for 90 days. result showed that striatum was damaged due a high dose brain, which characterized degeneration neurons astrocyte dysfunction. Transcriptome analysis demonstrated striatal astrocytes transformed A1 state under exposure. Deeper investigation revealed internalization gap junction protein connexin 43 responsible this transformation. Eventually, we conclude internalized anchoring, process accompanied transformation subtype. This study provides new direction exploring effects on nervous system treatment subsequent diseases.

Язык: Английский

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Genotoxicity responses of single and mixed exposure to heavy metals (cadmium, silver, and copper) as environmental pollutants in Drosophila melanogaster

Environmental Toxicology and Pharmacology, Год журнала: 2024, Номер 106, С. 104390 - 104390

Опубликована: Фев. 16, 2024

Язык: Английский

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Grandfathers-to-grandsons transgenerational transmission of exercise positive effects on cognitive performance

Journal of Neuroscience, Год журнала: 2024, Номер 44(23), С. e2061232024 - e2061232024

Опубликована: Май 6, 2024

Physical exercise is a robust lifestyle intervention known for its enhancement of cognitive abilities. Nevertheless, the extent to which these benefits can be transmitted across generations (intergenerational inheritance F1, and transgenerational F2 beyond) remains topic limited comprehension. We have already shown that improvements resulting from physical inherited parents their offspring, proving intergenerational effects. So, we set out explore whether enhancements might extend transgenerationally, impacting generation. In this study, initially examined behavioral traits second generation (F2) male mice, whose grandfathers (F0) had an intervention. Our findings revealed mice with physically active grandpaternal F0 progenitors displayed significantly improved memory recall, encompassing both spatial non-spatial information when compared counterparts sedentary progenitors, first time induced enhancement. Surprisingly, while (as was case F1), adult hippocampal neurogenesis remained unchanged between experimental control groups (unlike in F1). Additionally, our analysis small RNA sequences hippocampus identified 35 differentially expressed miRNAs linked important brain function categories. Notably, two miRNAs, miRNA-144 miRNA-298, negative correlation performance. These highlight enduring transmission associated exercise, even after generations, suggesting moderate training lasting positive effects, possibly orchestrated by specific exert influence multiple generations.

Язык: Английский

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