Quasi-LD-Targeted and ONOO^–-Responsive Fluorescent Probe for Investigating the Interaction of Nonalcoholic Fatty Liver with Drug-Induced Liver Injury

Analytical Chemistry, Год журнала: 2023, Номер 95(14), С. 5967 - 5975

Опубликована: Март 29, 2023

Hepatic lipid droplets (LDs) and peroxynitrite (ONOO-) levels are closely related to nonalcoholic fatty liver disease (NAFLD). Additionally, some drug-induced injury (DILI) is often associated with ONOO-. Here, we constructed screened the quasi-LDs-targeted ONOO--responsive fluorescent probe MBDP-Py+ investigate interaction of NAFLD DILI. By monitoring upregulation ONOO- accumulation LDs, was more sensitive efficient than tissue staining serum markers detection in evaluating early toxicity diagnosing anticancer-DILI. More importantly, enhancement fluorescence signals demonstrated that different stages NAFLD, dominant element combined DILI mice models. As degree deepens, synergistic effect two will lead serious damage.

Язык: Английский

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An ER‐targeted, Viscosity‐sensitive Hemicyanine Dye for the Diagnosis of Nonalcoholic Fatty Liver and Photodynamic Cancer Therapy by Activating Pyroptosis Pathway

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(9)

Опубликована: Янв. 10, 2024

The concept of molecular design, integrating diagnostic and therapeutic functions, aligns with the general trend modern medical advancement. Herein, we rationally designed smart molecule ER-ZS for endoplasmic reticulum (ER)-targeted diagnosis treatment in cell animal models by combining hemicyanine dyes ER-targeted functional groups (p-toluenesulfonamide). Owing to its ability target ER a highly specific response viscosity, demonstrated substantial fluorescence turn-on only after binding ER, independent other physiological environments. In addition, ER-ZS, being small molecule, allows nonalcoholic fatty liver disease (NAFLD) via imaging based on high stress. Importantly, is type I photosensitizer, producing O

Язык: Английский

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Multifunctional Fluorescent Probe for Simultaneous Detection of ONOO^–, Viscosity, and Polarity and Its Application in Ferroptosis and Cancer Models

Analytical Chemistry, Год журнала: 2023, Номер 95(13), С. 5780 - 5787

Опубликована: Март 20, 2023

Intracellular peroxynitrite anions (ONOO-) and microenvironments (such as viscosity polarity) play an important role in maintaining redox homeostasis, regulating diffusion, transportation, signal transduction living cells. The abnormality of these factors is often closely related to various physiological/pathological processes. However, owing the lack suitable probes, simultaneous visualization ONOO-, viscosity, polarity ferroptosis cancer models has not been achieved. To meet urgent needs, we presented a multifunctional near-infrared (NIR) fluorescent probe, named MQA-P, for simultaneously detecting within mitochondria. probe exhibited remarkable turn-on response ONOO- with far-red emission about 645 nm was highly sensitive viscosity/polarity NIR channel λem > 704 nm. Facilitated by first time, revealed that erastin-induced accompanied significant upregulation increase (or decrease at same time. Moreover, concurrent use diagnosis successfully achieved only cell/tissue levels but also tumor mice models. Compared one factor, this detection multimarkers provides more reliable method/tool tracking ferroptosis-related pathological processes diagnosis, holding great potential preclinical research, medical imaging-guided surgery.

Язык: Английский

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Simultaneous detection of peroxynitrite and viscosity in diabetes by a dual response fluorescent probe

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 406, С. 135432 - 135432

Опубликована: Фев. 2, 2024

Язык: Английский

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Engineering of a NIR fluorescent probe for high-fidelity tracking of lipid droplets in living cells and nonalcoholic fatty liver tissues

Biosensors and Bioelectronics, Год журнала: 2023, Номер 240, С. 115646 - 115646

Опубликована: Авг. 29, 2023

Язык: Английский

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Dual-channel fluorescent probe for simultaneously detecting H2S and viscosity/polarity and its application in non-alcoholic fatty liver, tumor tissue, and food spoilage

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 397, С. 134596 - 134596

Опубликована: Сен. 9, 2023

Язык: Английский

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Advances and perspectives in fluorescent probes for imaging hepatopathy-related biomarkers

Coordination Chemistry Reviews, Год журнала: 2023, Номер 497, С. 215407 - 215407

Опубликована: Сен. 12, 2023

Язык: Английский

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Nanomaterial-based chemiluminescence systems for tracing of reactive oxygen species in biosensors

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 162, С. 117020 - 117020

Опубликована: Март 13, 2023

Язык: Английский

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Multifunctional Mitochondria-Targeting Near-Infrared Fluorescent Probe for Viscosity, ONOO^–, Mitophagy, and Bioimaging

ACS Biomaterials Science & Engineering, Год журнала: 2023, Номер 9(6), С. 3581 - 3589

Опубликована: Май 30, 2023

Irregularities in mitochondrial viscosity and peroxynitrite (ONOO–) concentration can lead to dysfunction. It is still a great challenge develop near-infrared (NIR) fluorescent probes simultaneously detect viscosity, endogenous ONOO–, mitophagy. Herein, multifunctional mitochondria-targeting NIR probe P-1 was first synthesized for detecting used quinoline cations as targeting moiety, arylboronate an ONOO– responsive group, detected the change of by twisted internal charge transfer (TICT) mechanism. The has excellent response during inflammation lipopolysaccharides (LPSs) mitophagy induced starvation at 670 nm. changes nystatin zebrafish showed that able microviscosity vivo. also good sensitivity with detection limit 6.2 nM successfully applied zebrafish. Moreover, ability distinguish between cancer cells normal cells. All these features make promising candidate -associated physiological pathological processes.

Язык: Английский

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An ER‐targeted, Viscosity‐sensitive Hemicyanine Dye for the Diagnosis of Nonalcoholic Fatty Liver and Photodynamic Cancer Therapy by Activating Pyroptosis Pathway

Angewandte Chemie, Год журнала: 2024, Номер 136(9)

Опубликована: Янв. 10, 2024

Abstract The concept of molecular design, integrating diagnostic and therapeutic functions, aligns with the general trend modern medical advancement. Herein, we rationally designed smart molecule ER‐ZS for endoplasmic reticulum (ER)‐targeted diagnosis treatment in cell animal models by combining hemicyanine dyes ER‐targeted functional groups ( p ‐toluenesulfonamide). Owing to its ability target ER a highly specific response viscosity, demonstrated substantial fluorescence turn‐on only after binding ER, independent other physiological environments. In addition, , being small molecule, allows nonalcoholic fatty liver disease (NAFLD) via imaging based on high stress. Importantly, is type I photosensitizer, producing O 2 ⋅ − ⋅OH under light irradiation. Thus, irradiating certain period, photodynamic therapy inflicted severe oxidative damage tumor cells hypoxic (2 % ) conditions activated unique pyroptosis pathway, demonstrating excellent antitumor capacity xenograft models. Hence, proposed strategy will likely shed new optics NAFLD cancer therapy.

Язык: Английский

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