Quasi-LD-Targeted and ONOO^–-Responsive Fluorescent Probe for Investigating the Interaction of Nonalcoholic Fatty Liver with Drug-Induced Liver Injury

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(14), P. 5967 - 5975

Published: March 29, 2023

Hepatic lipid droplets (LDs) and peroxynitrite (ONOO-) levels are closely related to nonalcoholic fatty liver disease (NAFLD). Additionally, some drug-induced injury (DILI) is often associated with ONOO-. Here, we constructed screened the quasi-LDs-targeted ONOO--responsive fluorescent probe MBDP-Py+ investigate interaction of NAFLD DILI. By monitoring upregulation ONOO- accumulation LDs, was more sensitive efficient than tissue staining serum markers detection in evaluating early toxicity diagnosing anticancer-DILI. More importantly, enhancement fluorescence signals demonstrated that different stages NAFLD, dominant element combined DILI mice models. As degree deepens, synergistic effect two will lead serious damage.

Language: Английский

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An ER‐targeted, Viscosity‐sensitive Hemicyanine Dye for the Diagnosis of Nonalcoholic Fatty Liver and Photodynamic Cancer Therapy by Activating Pyroptosis Pathway

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(9)

Published: Jan. 10, 2024

The concept of molecular design, integrating diagnostic and therapeutic functions, aligns with the general trend modern medical advancement. Herein, we rationally designed smart molecule ER-ZS for endoplasmic reticulum (ER)-targeted diagnosis treatment in cell animal models by combining hemicyanine dyes ER-targeted functional groups (p-toluenesulfonamide). Owing to its ability target ER a highly specific response viscosity, demonstrated substantial fluorescence turn-on only after binding ER, independent other physiological environments. In addition, ER-ZS, being small molecule, allows nonalcoholic fatty liver disease (NAFLD) via imaging based on high stress. Importantly, is type I photosensitizer, producing O

Language: Английский

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Multifunctional Fluorescent Probe for Simultaneous Detection of ONOO^–, Viscosity, and Polarity and Its Application in Ferroptosis and Cancer Models

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(13), P. 5780 - 5787

Published: March 20, 2023

Intracellular peroxynitrite anions (ONOO-) and microenvironments (such as viscosity polarity) play an important role in maintaining redox homeostasis, regulating diffusion, transportation, signal transduction living cells. The abnormality of these factors is often closely related to various physiological/pathological processes. However, owing the lack suitable probes, simultaneous visualization ONOO-, viscosity, polarity ferroptosis cancer models has not been achieved. To meet urgent needs, we presented a multifunctional near-infrared (NIR) fluorescent probe, named MQA-P, for simultaneously detecting within mitochondria. probe exhibited remarkable turn-on response ONOO- with far-red emission about 645 nm was highly sensitive viscosity/polarity NIR channel λem > 704 nm. Facilitated by first time, revealed that erastin-induced accompanied significant upregulation increase (or decrease at same time. Moreover, concurrent use diagnosis successfully achieved only cell/tissue levels but also tumor mice models. Compared one factor, this detection multimarkers provides more reliable method/tool tracking ferroptosis-related pathological processes diagnosis, holding great potential preclinical research, medical imaging-guided surgery.

Language: Английский

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Simultaneous detection of peroxynitrite and viscosity in diabetes by a dual response fluorescent probe

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 406, P. 135432 - 135432

Published: Feb. 2, 2024

Language: Английский

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Engineering of a NIR fluorescent probe for high-fidelity tracking of lipid droplets in living cells and nonalcoholic fatty liver tissues

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 240, P. 115646 - 115646

Published: Aug. 29, 2023

Language: Английский

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Dual-channel fluorescent probe for simultaneously detecting H2S and viscosity/polarity and its application in non-alcoholic fatty liver, tumor tissue, and food spoilage

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 397, P. 134596 - 134596

Published: Sept. 9, 2023

Language: Английский

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Advances and perspectives in fluorescent probes for imaging hepatopathy-related biomarkers

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 497, P. 215407 - 215407

Published: Sept. 12, 2023

Language: Английский

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Nanomaterial-based chemiluminescence systems for tracing of reactive oxygen species in biosensors

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 162, P. 117020 - 117020

Published: March 13, 2023

Language: Английский

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Multifunctional Mitochondria-Targeting Near-Infrared Fluorescent Probe for Viscosity, ONOO^–, Mitophagy, and Bioimaging

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(6), P. 3581 - 3589

Published: May 30, 2023

Irregularities in mitochondrial viscosity and peroxynitrite (ONOO–) concentration can lead to dysfunction. It is still a great challenge develop near-infrared (NIR) fluorescent probes simultaneously detect viscosity, endogenous ONOO–, mitophagy. Herein, multifunctional mitochondria-targeting NIR probe P-1 was first synthesized for detecting used quinoline cations as targeting moiety, arylboronate an ONOO– responsive group, detected the change of by twisted internal charge transfer (TICT) mechanism. The has excellent response during inflammation lipopolysaccharides (LPSs) mitophagy induced starvation at 670 nm. changes nystatin zebrafish showed that able microviscosity vivo. also good sensitivity with detection limit 6.2 nM successfully applied zebrafish. Moreover, ability distinguish between cancer cells normal cells. All these features make promising candidate -associated physiological pathological processes.

Language: Английский

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An ER‐targeted, Viscosity‐sensitive Hemicyanine Dye for the Diagnosis of Nonalcoholic Fatty Liver and Photodynamic Cancer Therapy by Activating Pyroptosis Pathway

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(9)

Published: Jan. 10, 2024

Abstract The concept of molecular design, integrating diagnostic and therapeutic functions, aligns with the general trend modern medical advancement. Herein, we rationally designed smart molecule ER‐ZS for endoplasmic reticulum (ER)‐targeted diagnosis treatment in cell animal models by combining hemicyanine dyes ER‐targeted functional groups ( p ‐toluenesulfonamide). Owing to its ability target ER a highly specific response viscosity, demonstrated substantial fluorescence turn‐on only after binding ER, independent other physiological environments. In addition, , being small molecule, allows nonalcoholic fatty liver disease (NAFLD) via imaging based on high stress. Importantly, is type I photosensitizer, producing O 2 ⋅ − ⋅OH under light irradiation. Thus, irradiating certain period, photodynamic therapy inflicted severe oxidative damage tumor cells hypoxic (2 % ) conditions activated unique pyroptosis pathway, demonstrating excellent antitumor capacity xenograft models. Hence, proposed strategy will likely shed new optics NAFLD cancer therapy.

Language: Английский

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