Designing a CRISPR/Cas12a- and Au-Nanobeacon-Based Diagnostic Biosensor Enabling Direct, Rapid, and Sensitive miRNA Detection

Analytical Chemistry, Год журнала: 2022, Номер 94(17), С. 6566 - 6573

Опубликована: Апрель 22, 2022

Direct, rapid, sensitive, and selective detection of nucleic acids in complex biological fluids is crucial for medical early diagnosis. We herein combine the trans-cleavage ability clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a with Au-nanobeacon to establish a CRISPR-based biosensor, providing rapid miRNA high speed attomolar sensitivity. In this strategy, we first report that activity CRISPR/cas12a, which was previously reported be triggered only by target ssDNA or dsDNA, can activated directly. Therefore, method direct, i.e., does not need conversion into its complementary DNA (cDNA). Meanwhile, as compared traditional reporters molecular beacon (MB) reporters, exhibit improved reaction kinetics assay, miRNA-21 could detected very sensitivity 5 min. Finally, proposed strategy enables determination samples, potential tool

Язык: Английский

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Non-enzymatic signal amplification-powered point-of-care SERS sensor for rapid and ultra-sensitive assay of SARS-CoV-2 RNA

Biosensors and Bioelectronics, Год журнала: 2022, Номер 212, С. 114379 - 114379

Опубликована: Май 17, 2022

Язык: Английский

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Graphene-Based Field-Effect Transistors in Biosensing and Neural Interfacing Applications: Recent Advances and Prospects

Analytical Chemistry, Год журнала: 2023, Номер 95(5), С. 2590 - 2622

Опубликована: Янв. 24, 2023

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTGraphene-Based Field-Effect Transistors in Biosensing and Neural Interfacing Applications: Recent Advances ProspectsSiva Kumar Krishnan*Siva KrishnanCONACYT-Instituto de Física, Benemérita Universidad Autónoma Puebla, Apdo. Postal J-48, Puebla72570, Mexico*[email protected]More by Siva KrishnanView Biographyhttps://orcid.org/0000-0002-9672-9335, Nandini NatarajNandini NatarajDepartment of Chemical Engineering Biotechnology, National Taipei University Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei106, TaiwanMore NatarajView Biography, M. MeyyappanM. MeyyappanCentre for Nanotechnology, Indian Institute Guwahati781039, Assam, IndiaMore MeyyappanView Biographyhttps://orcid.org/0000-0001-9202-412X, Umapada Pal*Umapada PalInstituto PalView Biographyhttps://orcid.org/0000-0002-5665-106XCite this: Anal. Chem. 2023, 95, 5, 2590–2622Publication Date (Web):January 24, 2023Publication History Received4 August 2022Published online24 January 2023Published inissue 7 February 2023https://pubs.acs.org/doi/10.1021/acs.analchem.2c03399https://doi.org/10.1021/acs.analchem.2c03399review-articleACS PublicationsCopyright © 2023 American SocietyRequest reuse permissionsArticle Views2719Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Язык: Английский

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Recent progress in microfluidic biosensors with different driving forces

TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 158, С. 116894 - 116894

Опубликована: Дек. 23, 2022

Язык: Английский

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Framework‐Hotspot Enhanced Trans Cleavage of CRISPR‐Cas12a for Clinical Samples Detection

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(32)

Опубликована: Июнь 7, 2023

Abstract The trans‐cleavage property of CRISPR‐Cas12a system makes it an excellent tool for disease diagnosis. Nevertheless, most methods based on CRISPR‐Cas still require pre‐amplification the target to achieve desired detection sensitivity. Here we generate Framework‐Hotspot reporters (FHRs) with different local densities investigate their effect activity Cas12a. We find that cleavage efficiency increases and rate accelerates increasing reporter density. further construct a modular sensing platform CRISPR‐Cas12a‐based recognition FHR‐based signal transduction. Encouragingly, this enables sensitive (100 fM) rapid (<15 min) pathogen nucleic acids without pre‐amplification, as well tumor protein markers in clinical samples. design provides facile strategy enhanced trans Cas12a, which broadens its applications biosensing.

Язык: Английский

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A DNA Logic Circuit Equipped with a Biological Amplifier Loaded into Biomimetic ZIF‐8 Nanoparticles Enables Accurate Identification of Specific Cancers In Vivo

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(41)

Опубликована: Авг. 24, 2023

DNA logic circuits (DLC) enable the accurate identification of specific cell types, such as cancer cells, but they face challenges weak output signals and a lack competent platforms that can efficiently deliver DLC components to target site in living body. To address these issues, we rationally introduced cascaded biological amplifier module based on Primer Exchange Reaction inspired by electronic circuit devices. As paradigm, three abnormally expressed Hela microRNAs (-30a, -17, -21) were chosen "AND" gate inputs. response inputs was boosted markedly enhancing signal. More importantly, encapsulation into ZIF-8 nanoparticles resulted their efficient delivery site, successfully distinguishing tumor subtype from other tumors vivo. Thus, envision this strategy has great potential for clinical diagnosis.

Язык: Английский

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Bottom-Up Signal Boosting with Fractal Nanostructuring and Primer Exchange Reaction for Ultrasensitive Detection of Cancerous Exosomes

ACS Sensors, Год журнала: 2023, Номер 8(3), С. 1308 - 1317

Опубликована: Март 1, 2023

Exosomes are emerging as promising biomarkers for cancer diagnosis, yet sensitive and accurate quantification of tumor-derived exosomes remains a challenge. Here, we report an ultrasensitive specific exosome sensor (NPExo) that initially leverages hierarchical nanostructuring array primer exchange reaction (PER) quantitation cancerous exosomes. This NPExo uses high-curvature (bottom) fabricated by single-step electrodeposition to enhance capturing the target The immuno-captured thus provides abundant membrane sites insert numerous cholesterol-DNA probes with density much higher than immune pairing, which further allows PER-based DNA extension assemble enzyme concatemers (up) signal amplification. Such bottom-up signal-boosting design imparts ultrahigh sensitivity up 75 particles/mL (i.e., <1 per 10 μL) broad dynamic range spanning 6 orders magnitude. Furthermore, our monitoring subtle exosomal phenotypic transition shows high accuracy in discrimination liver patients from healthy donors via blood samples, suggesting great potential tool clinical diagnostics.

Язык: Английский

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Light-Driven Mitochondrion-to-Nucleus DNA Cascade Fluorescence Imaging and Enhanced Cancer Cell Photoablation

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(19), С. 12941 - 12949

Опубликована: Апрель 30, 2024

Nucleic acids are mainly found in the mitochondria and nuclei of cells. Detecting nucleic mitochondrion nucleus cascade mode is crucial for understanding diverse biological processes. This study introduces a novel acid-based fluorescent styrene dye (SPP) that exhibits light-driven migration from to nucleus. By introducing N-arylpyridine on one side skeleton bis(2-ethylsulfanyl-ethy)-amino unit other side, we SPP excellent DNA specificity (16-fold, FDNA/Ffree) stronger binding force nuclear (−5.09 kcal/mol) than mitochondrial (−2.59 kcal/mol). initially accumulates then migrates within 10 s under light irradiation. tracking damage apoptotic cells, allows successful visualization differences between apoptosis ferroptosis. Finally, triphenylamine segment with photodynamic effects was incorporated into form photosensitizer (MTPA-SPP), which targets photosensitization irradiation enhanced cancer cell treatment. innovative molecule light-triggered mitochondrion-to-nucleus ability provides feasible approach situ identification acids, monitoring subcellular physiological events, efficient therapy.

Язык: Английский

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Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells

Nature Protocols, Год журнала: 2024, Номер 19(4), С. 985 - 1014

Опубликована: Фев. 5, 2024

Язык: Английский

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DNA and Nanomaterials: A Functional Combination for DNA Sensing

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(2), С. 778 - 786

Опубликована: Янв. 25, 2024

Recent decades have experienced tough situations due to the lack of reliable diagnostic facilities. The most recent cases occurred during pandemic, where researchers observed facilities with precision. Microorganisms and viral disease's ability escape diagnosis has been a global challenge. DNA always unique moiety strong precise base-paired structure. in human foreign particles makes identification possible through base pairing. Since then, focused heavily on designing assays targeting particular. Moreover, nanotechnology contributed vastly composite nanomaterials by combining DNA/nucleic acids functional inorganic nanoparticles exploiting their physicochemical properties. These often exhibit or enhanced properties synergistic activity many components. capabilities additional shown combination robust advanced tailoring biosensors. Preceding findings state that conventional strategies exhibited certain limitations such as low range target detection, less biodegradability, subordinate half-life, high susceptibility microenvironments; however, DNA–nanomaterial-based biosensor overcome these meaningfully. Additionally, nucleic studied extensively signal conduction abilities. Here, we review studies biosensors, mechanism action, improved/updated vivo situ. Furthermore, this highlights methodologies utilization exploit interfacial sensing. Lastly, concludes limitations/challenges future directions.

Язык: Английский

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