Journal of Analytical Science & Technology,
Год журнала:
2023,
Номер
14(1)
Опубликована: Июнь 21, 2023
Abstract
The
aberrant
expression
of
microRNA
(miRNA)
is
closely
associated
with
various
pathological
processes,
such
as
the
development
gastric
cancer.
High-efficiency
quantification
miRNAs
significant
for
diagnosis,
prognosis,
and
treatment
cancers.
However,
sensitive
reliable
detection
miRNA
remains
a
huge
challenge.
We
depict
here
novel
fluorescent
approach
label-free
by
exploiting
designed
scaffold
to
integrate
catalytic
hairpin
assembly
primer
exchange
reaction
(PER).
In
this
method,
that
constructed
based
on
hybridization
between
two
structure
probes
(H1
probe
H2
probe),
capable
specifically
recognizing
target
activating
signal
amplification,
PER
process
transcribes
numerous
G-rich
sequences
induce
ThT-based
generation.
Based
efficient
amplification
strategy
generation
mode,
method
exhibits
wide
range
7
orders
magnitude
high
repeatability
(coefficient
variation,
2.76%),
implying
proposed
will
be
robust
tool
in
early
diagnosis
disease.
Graphical
abstract
ACS Nano,
Год журнала:
2024,
Номер
18(22), С. 14569 - 14582
Опубликована: Май 23, 2024
Accurately
assaying
tumor-derived
circulating
extracellular
vesicles
(EVs)
is
fundamental
in
noninvasive
cancer
diagnosis
and
therapeutic
monitoring
but
limited
by
challenges
efficient
EV
isolation
profiling.
Here,
we
report
a
bioinspired
buoyancy-driven
metal-organic
framework
(MOF)
corona
that
leverages
on-bubble
coordination
dual-encoded
surface-enhanced
Raman
scattering
(SERS)
nanotags
to
streamline
rapid
ultrasensitive
profiling
of
plasma
EVs
single
assay
for
diagnostics.
This
integrated
bubble-MOF-SERS
(IBMsv)
allows
barnacle-like
high-density
adhesion
MOFs
on
self-floating
bubble
surface
enable
fast
(2
min,
near
90%
capture
efficiency)
tumor
via
enhanced
EV-MOF
binding.
Also,
IBMsv
harnesses
four-plexed
SERS
profile
the
captured
protein
markers
at
single-particle
level.
Such
sensitive
multiplexed
across
five
types,
revealing
heterogeneous
expression
patterns.
Furthermore,
enables
pilot
clinical
cohort
(
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Фев. 8, 2024
Abstract
Background
Exosomes
are
nanoscale
extracellular
vesicles
(30–160
nm)
with
endosome
origin
secreted
by
almost
all
types
of
cells,
which
considered
to
be
messengers
intercellular
communication.
Cancerous
exosomes
serve
as
a
rich
source
biomarkers
for
monitoring
changes
in
cancer-related
physiological
status,
because
they
carry
large
number
biological
macromolecules
derived
from
parental
tumors.
The
ultrasensitive
quantification
trace
amounts
cancerous
is
highly
valuable
non-invasive
early
cancer
diagnosis,
yet
it
remains
challenging.
Herein,
we
developed
an
aptamer-carrying
tetrahedral
DNA
(Apt-TDNA)
microelectrode
sensor,
assisted
polydopamine
(PDA)
coating
semiconducting
properties,
the
electrochemical
detection
cancer-derived
exosomes.
Results
stable
rigid
structure
and
orientation
Apt-TDNA
ensured
efficient
capture
suspended
Without
PDA
signal
amplification
strategy,
sensor
has
linear
working
range
10
2
–10
7
particles
mL
−1
,
LOD
~
69
42
EIS
DPV,
respectively.
With
coating,
further
amplified,
achieving
single
particle
level
sensitivity
(~
14
6
DPV).
Conclusions
proposed
PDA-assisted
integrates
exosome
capture,
sensitive
feedback
amplification,
provides
new
avenue
development
simple
sensing
techniques
diagnosis
treatment
response.
Graphical
ACS Nano,
Год журнала:
2023,
Номер
17(10), С. 9633 - 9646
Опубликована: Май 5, 2023
Integrating
clinical
rare
cell
enrichment,
culture,
and
single-cell
phenotypic
profiling
is
currently
hampered
by
the
lack
of
competent
technologies,
which
typically
suffer
from
weak
cell–interface
collision
affinity,
strong
nonspecific
adsorption,
potential
uptake.
Here,
we
report
cells-on-a-bubble,
a
bioinspired,
self-powered
bioorthogonal
microbubble
(click
bubble)
that
leverages
clickable
antifouling
nanointerface
DNA-assembled
sucker-like
polyvalent
surface,
to
enable
instant
suspended
isolation
circulating
tumor
cells
(CTCs)
within
minutes.
Using
this
biomimetic
engineering
strategy,
click
bubbles
achieve
capture
efficiency
up
98%,
improved
20%
at
15
times
faster
over
their
monovalent
counterparts.
Further,
buoyancy-activated
bubble
facilitates
self-separation,
3D
suspension
in
situ
phenotyping
captured
single
cancer
cells.
By
using
multiantibody
design,
fast,
affordable
micromotor-like
enables
enrichment
CTCs
cohort
(n
=
42)
across
three
types
treatment
response
evaluation,
signifying
its
great
analysis
organoid
culture.
ACS Nano,
Год журнала:
2024,
Номер
18(45), С. 31174 - 31187
Опубликована: Ноя. 1, 2024
Multiple
and
ultrasensitive
detection
of
pathogenic
bacteria
is
critical
but
remains
a
challenge.
Here,
we
introduce
digital
assay
for
multiplexed
target
DNA
amplification-free
using
botryoidal-like
fluorescent
polystyrene
dots
(PS-dots),
which
were
first
prepared
through
the
hybridization
reaction
between
primer
exchange
chains
nanospheres
that
encapsulated
polymer
signal
preamplification.
The
bacteria's
was
cleavaged
by
argonaute
(Ago)
protein-mediated
multiple
precise
cleavage
reactions,
where
obtained
sequences
bridged
magnetic
beads
(MBs)
PS-dots
via
reaction,
MB-botryoidal
PS-dot
complexes
utilized
as
probes
based
on
colors
sizes
encoding.
An
artificial-intelligence-fluorescent
microsphere
counting
algorithm
applied
to
identify
count
MBs
readout.
This
combined
ultrabright
with
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
The
unmet
on‐site
clinical
diagnostics
are
increasingly
demanding
portable
bioassays,
but
compromised
by
sensitivity
and
throughput.
Herein,
a
nanostructured
bubbles‐enhanced
fluorescence
(nBEF)
assay
is
reported
interfacing
with
smartphone
imaging
system
to
enable
portable,
ultrasensitive,
specific
miRNA
detection
in
parallel.
In
nBEF,
the
high‐curvature
bubbles
allow
physically
enhancing
optical
propagation,
chemically
fluorescence,
assembling
more
DNA
probes,
undergoing
faster
self‐suspension
efficient
self‐aggregation
enhancement
relative
their
smooth
counterparts.
By
coupling
bubble‐driven
signal
amplification
plug‐and‐play
concatemer,
system,
this
nBEF
achieves
ultrasensitive
fluorescent
of
at
femtomolar
level
one‐base
resolution
single
test,
capable
monitoring
drug‐induced
dynamic
expression
accurate
cancer
diagnosis
(AUC
=
1)
through
profiling
40
plasma
samples
after
RNA
extraction.
This
further
integrates
an
R‐value
analysis
RGB
module
realize
automated,
readout.
Such
high‐performance
provides
low‐cost,
highly
sensitive
tool
diagnosis.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 2, 2025
Flap
endonuclease
1
(FEN1)
is
a
specific
enzyme
capable
of
recognizing
and
cleaving
triplex
DNA
structures
releasing
5'-flap
fragments.
It
plays
crucial
role
in
the
metabolism
cells,
participating
replication
repair
damaged
DNA.
Additionally,
FEN1
overexpressed
various
tumor
tissues,
promoting
progression
drug
resistance
through
different
regulatory
mechanisms.
However,
few
significant
advancements
have
been
made
sensitive
analytical
methods
for
detecting
FEN1.
Herein,
this
study,
we
present
highly
flow
cytometry
biosensor
with
solid-phase
interface-mediated
primer
exchange
reaction
amplification
(FCsperA)
analysis.
By
comparing
homogeneous
PER
(h-PER),
found
that
(s-PER)
effectively
suppressed
background
signals,
leading
to
higher
signal-to-background
(S/B)
ratio
exceeding
∼46-fold
when
was
at
×
10
