ACS Omega,
Год журнала:
2024,
Номер
9(47), С. 47342 - 47349
Опубликована: Ноя. 12, 2024
Herein,
we
report
a
naturally
occurring
flavonol,
quercetagetin
5,6,7,3′,4′-pentamethyl
ether
(known
as
marionol),
nontoxic
fluorescent
probe
for
rapid,
sensitive,
and
selective
detection
of
Cu2+
in
water.
The
interaction
between
marionol
ions
is
studied
by
various
state-of-the-art
spectroscopic
techniques
such
1H
NMR,
UV–vis,
fluorescence,
high-resolution
mass
spectrometry
(HRMS).
Marionol
shows
strong
fluorescence
quenching
the
presence
Cu2+,
whereas
other
interfering
cations
Fe3+,
Co2+,
Al3+,
Pb2+,
Hg2+,
Zn2+,
Mg2+,
Mn2+,
Ca2+,
Cu+,
Ag+,
Na+,
K+
produce
negligible
changes
fluorescence.
binding
stoichiometry
found
to
be
2:1,
according
Job's
plot
HRMS
analyses.
constant
(Ka)
with
calculated
2.89
×
109
M–2,
limit
0.15
μM.
In
addition,
further
used
quantification
natural
spring
water
samples.
good
recoveries
(92–110%
RSD
<5%)
its
low
toxicity
MCF-7
human
breast
cancer
cells
make
unique
plant-based
ions.
Metal
ions
are
essential
for
all
life.
In
microbial
cells,
potassium
(K+)
is
the
most
abundant
cation
and
plays
a
key
role
in
maintaining
osmotic
balance.
Magnesium
(Mg2+)
dominant
divalent
required
nucleic
acid
structure
as
an
enzyme
cofactor.
Microbes
typically
require
transition
metals
manganese
(Mn),
iron
(Fe),
copper
(Cu),
zinc
(Zn),
although
precise
set
of
metal
needed
to
sustain
life
variable.
Intracellular
pools
can
be
conceptualized
chemically
complex
mixture
rapidly
exchanging
(labile)
ions,
complemented
by
those
reservoirs
that
exchange
slowly
relative
cell
metabolism
(sequestered).
Labile
buffered
transient
interactions
with
anionic
metabolites
macromolecules,
ribosome
playing
major
role.
Sequestered
include
many
metalloproteins,
cofactors,
storage
depots,
some
redeployed
upon
depletion.
Here,
I
review
size,
composition,
dynamics
intracellular
highlight
gaps
understanding.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 3, 2024
A
comprehensive
overview
of
recent
advancements
in
fluorescence
imaging
techniques
for
situ
sensing
various
biomarkers,
emphasizing
the
transformative
potential
artificial
intelligence
shaping
future
bioimaging.
Proceedings of the National Academy of Sciences,
Год журнала:
2025,
Номер
122(3)
Опубликована: Янв. 15, 2025
Copper
is
an
essential
nutrient
for
sustaining
vital
cellular
processes
spanning
respiration,
metabolism,
and
proliferation.
However,
loss
of
copper
homeostasis,
particularly
misregulation
loosely
bound
ions
which
are
defined
as
the
labile
pool,
occurs
in
major
diseases
such
cancer,
where
tumor
growth
metastasis
have
a
heightened
requirement
this
metal.
To
help
decipher
role
etiology
we
report
histochemical
activity-based
sensing
approach
that
enables
systematic,
high-throughput
profiling
status
across
many
cell
lines
parallel.
Coppermycin-1
reacts
selectively
with
Cu(I)
to
release
puromycin,
then
incorporated
into
nascent
peptides
during
protein
translation,
thus
leaving
permanent
dose-dependent
marker
can
be
visualized
standard
immunofluorescence
assays.
We
showcase
utility
platform
screening
pools
National
Cancer
Institute’s
60
(NCI-60)
human
line
panel,
identifying
types
elevated
basal
levels
copper.
Moreover,
use
show
lung
cancer
cells
activation
nuclear
factor-erythroid
2-related
factor
2
(NRF2)
possess
lower
resting
and,
result,
reduced
viability
when
treated
chelator.
This
work
establishes
methods
detection
used
assess
cuproplasia,
emerging
form
copper-dependent
proliferation,
providing
starting
point
broader
investigations
roles
transition
metal
signaling
biology
medicine.
ACS Applied Energy Materials,
Год журнала:
2025,
Номер
8(4), С. 1942 - 1963
Опубликована: Фев. 6, 2025
This
review
article
reports
an
overview
of
the
recent
developments
in
field
electron
delocalization
study
organic
conjugated
molecules
by
utilizing
vibration
frequencies
exhibited
attached
functional
groups
such
as
nitrile
(−C≡N),
alkyne
(−C≡C−),
or
carbonyl
(−C═O).
A
brief
introduction
to
delocalization,
methods
for
study,
and
their
importance
is
given
first,
followed
application
infrared
spectroscopy
molecules.
Details
with
various
reporter
have
been
explained
respective
subsections
based
on
groups.
All
reported
structured
presented
properties
studied
using
group.
Finally,
outlook
this
recently
promising,
exciting,
interesting
probing
provided.
