Extracellular vimentin is a damage-associated molecular pattern protein serving as an agonist of TLR4 in human neutrophils

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 5, 2025

Язык: Английский

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Human embryo live imaging reveals nuclear DNA shedding during blastocyst expansion and biopsy

Cell, Год журнала: 2023, Номер 186(15), С. 3166 - 3181.e18

Опубликована: Июль 1, 2023

Язык: Английский

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Morphogens enable interacting supracellular phases that generate organ architecture

Science, Год журнала: 2023, Номер 382(6673)

Опубликована: Ноя. 23, 2023

During vertebrate organogenesis, increases in morphological complexity are tightly coupled to morphogen expression. In this work, we studied how morphogens influence self-organizing processes at the collective or “supra”-cellular scale avian skin. We made physical measurements across length scales, which revealed morphogen-enabled material property differences that were amplified supracellular scales comparison cellular scales. At scale, found fibroblast growth factor (FGF) promoted “solidification” of tissues, whereas bone morphogenetic protein (BMP) fluidity and enhanced mechanical activity. Together, these effects created basement membrane–less compartments within mesenchymal tissue mechanically primed drive skin budding. Understanding multiscale process requires ability distinguish between proximal occur their functional effects, emerge scale.

Язык: Английский

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Cytoskeletal crosstalk: A focus on intermediate filaments

Current Opinion in Cell Biology, Год журнала: 2024, Номер 87, С. 102325 - 102325

Опубликована: Фев. 14, 2024

The cytoskeleton, comprising actin microfilaments, microtubules, and intermediate filaments, is crucial for cell motility tissue integrity. While prior studies largely focused on individual cytoskeletal networks, recent research underscores the interconnected nature of these systems in fundamental cellular functions like adhesion, migration, division. Understanding coordination distinct networks both time space essential. This review synthesizes current findings intricate interplay between emphasizing pivotal role filaments. Notably, filaments engage extensive crosstalk with microfilaments microtubules through direct molecular interactions, linkers, motors that form bridges, as well via more complex regulation intracellular signaling.

Язык: Английский

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Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM

The Journal of Cell Biology, Год журнала: 2025, Номер 224(4)

Опубликована: Март 10, 2025

Vimentin intermediate filaments (VIFs) form complex, tightly packed networks; due to this density, traditional imaging approaches cannot discern single-filament behavior. To address this, we developed and validated a sparse vimentin-SunTag labeling strategy, enabling single-particle tracking of individual VIFs providing sensitive, unbiased, quantitative method for measuring global VIF motility. Using approach, define the steady-state motility rate, showing constant ∼8% undergo directed microtubule-based motion irrespective subcellular location or local filament density. Significantly, our approach revealed uncorrelated within bundles, an observation seemingly at odds with conventional models cross-linked bundles. acquired high-resolution focused ion beam scanning electron microscopy volumes vitreously frozen cells reconstructed three-dimensional finding that they only loosely organized, semi-coherent structures from which single frequently emerge locally engage neighboring microtubules. Overall, work demonstrates dynamics organization in cellular milieu first time.

Язык: Английский

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Keratin filament mechanics and energy dissipation are determined by metal-like plasticity

Matter, Год журнала: 2023, Номер 6(6), С. 2019 - 2033

Опубликована: Май 22, 2023

Cell mechanics are determined by an intracellular biopolymer network, including intermediate filaments that expressed in a cell-type-specific manner. A prominent pair of keratin and vimentin, as they non-motile motile cells, respectively. Therefore, the differential expression these proteins coincides with change cellular dynamic properties cells. This observation raises question how mechanical already differ on single filament level. Here, we use optical tweezers computational model to compare stretching dissipation behavior two types. We find vimentin behave opposite ways: elongate but retain their stiffness, whereas soften length. finding is explained fundamentally different ways dissipate energy: viscous sliding subunits within non-equilibrium α helix unfolding filaments.

Язык: Английский

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Vimentin at the core of wound healing

Trends in Cell Biology, Год журнала: 2023, Номер 34(3), С. 239 - 254

Опубликована: Сен. 23, 2023

Язык: Английский

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Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation

Journal of The Royal Society Interface, Год журнала: 2024, Номер 21(215)

Опубликована: Июнь 1, 2024

Cell polarity is important for controlling cell shape, motility and division processes. Vimentin intermediate filaments are migration polarization in mesenchymal cells assembly of vimentin microtubule networks dynamically coordinated, but the precise details how mediates remain unclear. Here, we characterize effects on structure function centrosome stability wild-type vimentin-null mouse embryonic fibroblasts. We find that pericentriolar material, promotes centrosome-mediated regrowth increases level stable acetylated microtubules cell. Loss also impairs repositioning during processes occur wound closure. Our results suggest modulates as well network stability, which has implications establish proper persistent migration.

Язык: Английский

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Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures

Journal of Biological Chemistry, Год журнала: 2023, Номер 299(8), С. 104963 - 104963

Опубликована: Июнь 24, 2023

Vimentin intermediate filaments form part of the cytoskeleton mesenchymal cells, but under pathological conditions often associated with inflammation, vimentin depolymerize as result phosphorylation or citrullination, and oligomers are secreted released into extracellular environment. In space, can bind surfaces cells matrix, interaction between trigger changes in cellular functions, such activation fibroblasts to a fibrotic phenotype. The mechanism by which binds external cell membranes whether alone act an adhesive anchor for is largely uncharacterized. Here, we show that various types (normal null fibroblasts, stem A549 lung carcinoma cells) attach spread on polyacrylamide hydrogel substrates covalently linked vimentin. Using traction force microscopy spheroid expansion assays, characterize how different respond Cell attachment spreading vimentin-coated inhibited hyaluronic acid degrading enzymes, synthase inhibitors, soluble heparin N-acetyl glucosamine, all treatments have little no effect same binding collagen-coated hydrogels. These studies highlight effectiveness substrate-bound ligand suggest carbohydrate structures, including glycocalyx glycosylated surface proteins contain novel class adhesion receptors either directly support substrate fine-tune properties.

Язык: Английский

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Reconstitution of cytolinker-mediated crosstalk between actin and vimentin

European Journal of Cell Biology, Год журнала: 2024, Номер 103(2), С. 151403 - 151403

Опубликована: Март 12, 2024

Cell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, intermediate filaments. The biophysical properties each filament type individually have been studied extensively cell-free reconstitution. By contrast, interactions between three cytoskeletal networks relatively unexplored. They coupled via crosslinkers plakin family such as plectin. These challenging proteins for reconstitution because their giant size multidomain structure. Here we engineer a recombinant actin-vimentin crosslinker protein called 'ACTIF' that provides minimal model system plectin, recapitulating its modular design with actin-binding filament-binding domains separated coiled-coil linker dimerisation. We show fluorescence electron microscopy ACTIF has high binding affinity vimentin creates mixed bundles. Rheology measurements ACTIF-mediated crosslinking strongly stiffens composites. Finally, demonstrate modularity this approach creating variant domain Adenomatous Polyposis Coli. Our engineering new characterization understanding mechanical synergy in mesenchymal cells.

Язык: Английский

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