Foods,
Год журнала:
2022,
Номер
11(4), С. 544 - 544
Опубликована: Фев. 14, 2022
Food
safety
issues
are
a
worldwide
concern.
Pathogens,
toxins,
pesticides,
veterinary
drugs,
heavy
metals,
and
illegal
additives
frequently
reported
to
contaminate
food
pose
serious
threat
human
health.
Conventional
detection
methods
have
difficulties
fulfilling
the
requirements
for
development
in
modern
society.
Therefore,
novel
rapid
urgently
needed
on-site
screening
of
massive
samples.
Due
extraordinary
properties
nanozymes
aptamers,
biosensors
composed
both
them
provide
considerable
advantages
analytical
performances,
including
sensitivity,
specificity,
repeatability,
accuracy.
They
considered
promising
complementary
method
on
top
conventional
ones
accurate
contaminants.
In
recent
years,
we
witnessed
flourishing
strategies
based
aptamers
contaminants,
especially
models
regulation
by
single-stranded
DNA
(ssDNA)
nanozyme
activity.
However,
applications
nanozyme-based
aptasensors
seldom
reviewed.
Thus,
this
paper
aims
comprehensive
review
safety,
which
arranged
according
different
interaction
modes
ssDNA
nanozymes:
activity
either
inhibited
or
enhanced
ssDNA,
as
signal
tags,
other
methods.
Before
introducing
aptasensors,
via
diverse
factors
is
discussed
systematically
precisely
tailoring
biosensors.
Furthermore,
current
challenges
emphasized,
future
perspectives
discussed.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(17), С. 7053 - 7061
Опубликована: Апрель 20, 2023
False
positives
and
negatives
in
bioanalytical
assays
remain
a
persistent
problem.
Herein,
multifunctional
photoelectrochemical
(PEC)
biosensor
based
on
ZnIn2S4
(ZIS)/ZnS
quantum
dots
(QDs)@Au–Ag-reversed
photocurrent
of
Cu-metal–organic
framework
(MOF)
coupled
with
CRISPR/Cas-12a-shearing
was
innovatively
developed
for
assay
dual
targets.
First,
Cu-MOF
as
good
PEC
material
shows
cathodic
photocurrent.
Then,
numerous
ZIS/ZnS
QDs
were
assembled
to
the
Au–Ag
nanoparticles
(NPs)
prepare
stable
highly
amplified
signal
probe,
which
can
just
match
energy
level
Cu-MOFs
realized
polarity-reversed
first
time.
As
empty-core
nanostructure
NPs
has
high
specific
surface
area
low
density,
bimetallic
nanocrystal
much
increase
reaction
rate
improve
redox
efficiency.
When
target
CEA-produced
cDNA
opened
hairpin
DNA
(HP1
DNA)
electrode,
QDs@Au–Ag
probe
conjugated
electrode
via
hybridization,
achieving
significantly
reversed
current
CEA
detection.
Moreover,
binding
kanamycin/aptamer
generated
acDNA
(activator),
activate
trans-cleavage
activity
CRISPR-CAS12a
system
ssDNA,
so
sheared
caused
obvious
decrease
kanamycin
The
newly
displayed
excellent
properties
MOF
combined
unique
CRISPR-Cas12a
achieve
sensitive
detection
targets,
open
new
sensing
platform
rapid
accurate
analysis
multiple
targets
effectively
avoid
false
results
clinical
testing.
The
burden
of
food
contamination
and
wastage
has
significantly
contributed
to
the
increased
prevalence
foodborne
disease
insecurity
all
over
world.
Due
this,
there
is
an
urgent
need
develop
a
smarter
traceability
system.
Recent
advancements
in
biosensors
that
are
easy-to-use,
rapid
yet
selective,
sensitive,
cost-effective
have
shown
great
promise
meet
critical
demand
for
onsite
immediate
diagnosis
treatment
safety
quality
control
(i.e.
point-of-care
technology).
This
review
article
focuses
on
recent
development
different
monitoring.
In
general,
application
agriculture
pre-harvest
stage)
early
detection
routine
plant
infections
or
stress
discussed.
Afterward,
more
detailed
advancement
past
five
years
within
supply
chain
post-harvest
detect
types
contaminants
smart
packaging
highlighted.
A
section
discusses
perspectives
future
also
mentioned.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2021,
Номер
9
Опубликована: Авг. 24, 2021
Nanozymes
own
striking
merits,
including
high
enzyme-mimicking
activity,
good
stability,
and
low
cost.
Due
to
the
powerful
distinguished
functions,
nanozymes
exhibit
widespread
applications
in
field
of
biosensing
immunoassay,
attracting
researchers
various
fields
design
engineer
nanozymes.
Recently,
have
been
innovatively
used
bridge
nanotechnology
with
analytical
techniques
achieve
sensitivity,
specificity,
reproducibility.
However,
food
are
seldom
reviewed.
In
this
review,
we
summarize
several
typical
provide
a
comprehensive
description
history,
principles,
designs,
nanozyme-based
contaminants
detection.
Based
on
engineering
modification
nanozymes,
classified
then
discussed
detail
via
discriminating
roles
methods,
fluorescence,
colorimetric
electrochemical
assay,
surface-enhanced
Raman
scattering,
magnetic
relaxing
sensing,
electrochemiluminescence.
Further,
representative
examples
nanozymes-based
methods
highlighted
for
analysis
inhibition.
Finally,
current
challenges
prospects
discussed.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(16), С. 6410 - 6416
Опубликована: Апрель 14, 2022
Based
on
luminol-capped
Pt-tipped
Au
bimetallic
nanorods
(NRs)
(L-Au-Pt
NRs)
as
the
anode
emitter
and
SnS2
quantum
dots
(QDs)
hybrid
Eu
metal
organic
frameworks
(MOFs)
(SnS2
QDs@Eu
MOFs)
cathode
emitter,
a
dual-signal
electrochemiluminescence
(ECL)
platform
was
designed
for
ultrasensitive
highly
selective
detection
of
kanamycin
(KAN).
Using
output
mode,
ratiometric
ECL
aptasensor
largely
eliminates
false-positives
or
false-negatives
by
self-calibration
in
KAN
assay
process.
To
stimulate
resonance
energy
transform
(RET)
system,
aptamer
complementary
DNA
are
introduced
conjugation
between
donor
acceptor.
With
specific
recognition
target
its
aptamer,
L-Au-Pt
NRs-apt
partially
peels
off
from
electrode
surface.
Eventually,
RET
system
is
removed,
leading
to
an
increasing
signal
decreasing
signal.
In
view
this
phenomenon,
can
quantify
1
pM
10
nM
with
low
limit
0.32
pM.
This
exhibits
great
practical
potential
environmental
monitoring
food
safety.
RSC Advances,
Год журнала:
2022,
Номер
12(40), С. 26160 - 26175
Опубликована: Янв. 1, 2022
We
present
a
discussion
on
different
types
of
sensors
used
in
food
biomarker
detection
and
highlight
recent
studies
nanozyme-based
to
detect
markers
like
toxins,
pathogens,
antibiotics,
growth
hormones,
metal
ions,
additives,
small
molecules,
drug
residues.
