Virus-Mimicking Polymer Nanocomplexes Co-Assembling HCV E1E2 and Core Proteins with TLR 7/8 Agonist—Synthesis, Characterization, and In Vivo Activity

Journal of Functional Biomaterials, Год журнала: 2025, Номер 16(1), С. 34 - 34

Опубликована: Янв. 19, 2025

Hepatitis C virus (HCV) is a major public health concern, and the development of an effective HCV vaccine plays important role in effort to prevent new infections. Supramolecular co-assembly co-presentation envelope E1E2 heterodimer complex core protein presents attractive design strategy for achieving humoral cellular immunity. With this objective, two antigens were non-covalently assembled with immunostimulant (TLR 7/8 agonist) into virus-mimicking polymer nanocomplexes (VMPNs) using biodegradable synthetic polyphosphazene delivery vehicle. The resulting assemblies characterized dynamic light scattering asymmetric flow field-flow fractionation methods directly visualized their vitrified state by cryogenic electron microscopy. vivo superiority VMPNs over individual components Alum-formulated manifests higher neutralizing antibody titers, promotion balanced IgG response, induction immunity—CD4+ T cell responses proteins. aqueous-based spontaneous immunopotentiating molecules enabled carrier offers simple pathway polymer-based supramolecular nanovaccine systems.

Язык: Английский

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Nano Drug-Delivery Systems for Management of AIDS: Liposomes, Dendrimers, Gold and Silver Nanoparticles

Nanomedicine, Год журнала: 2023, Номер 18(3), С. 279 - 302

Опубликована: Фев. 1, 2023

AIDS causes increasing mortality every year. With advancements in nanomedicine, different nanomaterials (NMs) have been applied to treat and overcome its limitations. Among NMs, nanoparticles (NPs) can act as nanocarriers due their enhanced solubility, sustained release, targeting abilities facilitation of drug-dose reductions. This review discusses recent therapeutics for AIDS/HIV using various mainly focused on three classifications: polymeric, liposomal inorganic NMs. Polymeric dendrimers, polyethylenimine-NPs, poly(lactic-co-glycolic acid)-NPs, chitosan the use liposomal-based delivery systems NPs, including gold silver are explored. Recent advances, current challenges future perspectives these NMs better management HIV/AIDS also discussed.

Язык: Английский

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Nanoparticle-based strategies to target HIV-infected cells

Colloids and Surfaces B Biointerfaces, Год журнала: 2022, Номер 213, С. 112405 - 112405

Опубликована: Фев. 10, 2022

Язык: Английский

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Polyester-based long acting injectables: Advancements in molecular dynamics simulation and technological insights

Drug Discovery Today, Год журнала: 2022, Номер 28(2), С. 103463 - 103463

Опубликована: Дек. 5, 2022

Язык: Английский

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Temozolomide Efficacy and Metabolism: The Implicit Relevance of Nanoscale Delivery Systems

Molecules, Год журнала: 2022, Номер 27(11), С. 3507 - 3507

Опубликована: Май 30, 2022

The most common primary malignant brain tumors in adults are gliomas. Glioblastoma is the prevalent and aggressive tumor subtype of glioma. Current standards for treatment glioblastoma include a combination surgical, radiation, drug therapy methods. currently includes temozolomide (TMZ), an alkylating agent, bevacizumab, recombinant monoclonal IgG1 antibody that selectively binds to inhibits biological activity vascular endothelial growth factor. Supplementation radiation with TMZ increased patient survival from 12.1 14.6 months. specificity effect on largely determined by special aspects its pharmacokinetics. orally bioavailable prodrug, which well absorbed gastrointestinal tract converted active metabolite 5-(3-methyl triazen-1-yl)imidazole-4-carbozamide (MTIC) spontaneously physiological condition does not require hepatic involvement. MTIC produced plasma able cross BBB formed locally brain. A promising way increase effectiveness chemotherapy prevent hydrolysis peripheral tissues thereby concentration nanoscale delivery systems can provide. review discusses possible ways efficacy using nanocarriers.

Язык: Английский

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Biomaterials that passively and actively target macrophages promote the regeneration of injured tissues

Biomedical Technology, Год журнала: 2024, Номер 8, С. 17 - 49

Опубликована: Окт. 1, 2024

Язык: Английский

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Polymeric and lipidic nanoparticles in transforming anti-HIV combinational therapy: can they turn the tide?

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 23, 2025

Язык: Английский

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Role of Siglecs in viral infections: A double-edged sword interaction

Molecular Aspects of Medicine, Год журнала: 2022, Номер 90, С. 101113 - 101113

Опубликована: Авг. 16, 2022

Sialic-acid-binding immunoglobulin-like lectins are cell surface immune receptors known as Siglecs that play a paramount role modulators of immunity. In recent years, research has underscored how the underlaying biology this family influences outcome viral infections. While needed to promote effective antiviral responses, they can also pave way dissemination within tissues. Here, we review preclinical findings focusing on interplay between and viruses may translate into promising broad-spectrum therapeutic interventions or key biomarkers monitor course

Язык: Английский

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Membrane fluidity properties of lipid-coated polylactic acid nanoparticles

Nanoscale, Год журнала: 2024, Номер 16(17), С. 8533 - 8545

Опубликована: Янв. 1, 2024

Lipid coating is considered a versatile strategy to equip nanoparticles (NPs) with biomimetic surface coating, but the membrane properties of these nanoassemblies remain in many cases insufficiently understood. In this work, we apply C-Laurdan generalized polarization (GP) measurements probe temperature-dependent polarity hybrid membranes consisting lipid monolayer adsorbed onto polylactic acid (PLA) polymer core as function composition and compare behavior coated NPs (LNPs) that liposomes assembled from identical mixtures. The LNPs were generated by nanoprecipitation aqueous solutions containing two types mixtures: (i) cholesterol, dipalmitoylphosphatidylcholine (DPPC), ganglioside GM3, well (ii) dioleoylphosphatidylcholine (DOPC), DPPC GM3. found exhibit more distinct narrower phase transitions than corresponding retain detectable even for cholesterol or DOPC concentrations yielded no liposomes. These findings together higher GP values case temperatures above transition temperature indicate stabilization through core. LNP binding studies GM3-recognizing cells differences fluidity affect avidity investigated model system.

Язык: Английский

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Nonviral targeted mRNA delivery: principles, progresses, and challenges

MedComm, Год журнала: 2025, Номер 6(1)

Опубликована: Янв. 1, 2025

Abstract Messenger RNA (mRNA) therapeutics have garnered considerable attention due to their remarkable efficacy in the treatment of various diseases. The COVID‐19 mRNA vaccine and RSV been approved on market. Due inherent nuclease‐instability negative charge mRNA, delivery systems are developed protect from degradation facilitate its crossing cell membrane express functional proteins or peptides cytoplasm. However, deficiency transfection efficiency targeted biological distribution still major challenges for systems. In this review, we first described physiological barriers process then discussed design approach recent advances with an emphasis tissue/cell‐targeted abilities. Finally, pointed out existing future directions deep insights into efficient We believe that a high‐precision system can greatly improve therapeutic effects bio‐safety accelerate clinical transformations. This review may provide new direction serve as useful guide researchers who looking suitable system.

Язык: Английский

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