Integrating Artificial DNAzymes with Natural Enzymes on 2D MOF Hybrid Nanozymes for Enhanced Treatment of Bacteria‐Infected Wounds DOI

Fayin Mo,

Chuyan Lin,

Jing Lü

и другие.

Small, Год журнала: 2023, Номер 20(21)

Опубликована: Ноя. 28, 2023

Abstract Removal of invasive bacteria is critical for proper wound healing. This task challenging because these can trigger intense oxidative stress and gradually develop antibiotic resistance. Here, the use a multienzyme‐integrated nanocatalytic platform reported efficient bacterial clearance mitigation inflammatory responses, constructed by physically adsorbing natural superoxide dismutase (SOD), in situ reduction gold nanoparticles (Au NPs), incorporation DNAzyme on 2D NiCoCu metal–organic frameworks (DNAzyme/SOD/Au@NiCoCu MOFs, termed DSAM), which adapt to infected wounds. O 2 H replenishment achieved alleviated hypoxic microenvironment using antioxidant properties SOD. The produced during reaction decomposed peroxidase (POD)‐like activity enhanced Au NPs DNAzyme, releasing highly toxic hydroxyl radicals (•OH) kill bacteria. In addition, it possesses glutathione (GPx)‐like activity, depletes GSH prevents •OH loss. Systematic antimicrobial tests are performed against this nanoplatform. A dual‐mode strategy involving enzyme‐enhanced capacity artificial release an novel enzyme‐integrated therapeutic integrated.

Язык: Английский

Ultrasound-Augmented Multienzyme-like Nanozyme Hydrogel Spray for Promoting Diabetic Wound Healing DOI
Limin Shang, Yixin Yu, Yujie Jiang

и другие.

ACS Nano, Год журнала: 2023, Номер 17(16), С. 15962 - 15977

Опубликована: Авг. 3, 2023

Treatment of diabetic foot ulcers (DFU) needs to reduce inflammation, relieve hypoxia, lower blood glucose, promote angiogenesis, and eliminate pathogenic bacteria, but the therapeutic efficacy is greatly limited by diversity synergy drug functions as well DFU microenvironment itself. Herein, an ultrasound-augmented multienzyme-like nanozyme hydrogel spray was developed using hyaluronic acid encapsulated l-arginine ultrasmall gold nanoparticles Cu1.6O coloaded phosphorus doped graphitic carbon nitride nanosheets (ACPCAH). This possesses five types enzyme-like activities, including superoxide dismutase (SOD)-, catalase (CAT)-, glucose oxidase (GOx)-, peroxidase (POD)-, nitric oxide synthase (NOS)-like activities. The kinetics reaction mechanism sonodynamic/sonothermal synergistic enhancement SOD-CAT-GOx-POD/NOS cascade ACPCAH are fully investigated. Both in vitro vivo tests demonstrate that this can be activated thus accelerating wound healing effectively. study highlights a competitive approach based on nanozymes for development all-in-one therapies.

Язык: Английский

Процитировано

112
Photodynamic Alginate Zn-MOF Thermosensitive Hydrogel for Accelerated Healing of Infected Wounds DOI

Wenshang Zhang,

Bingjie Wang, Guangli Xiang

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(19), С. 22830 - 22842

Опубликована: Май 2, 2023

Antibiotic resistance reduces the effectiveness of infected wound healing, and it is necessary to develop a new strategy promote healing without using antibiotics. Here, we Chlorin e6 (Ce6)-loaded zinc-metal-organic framework (MOF) thermosensitive hydrogel (Ce6@MOF-Gel) based on alginate poly(propylene glycol) 407, which enhances antibacterial effects promotes by novel combining zinc-MOF with photodynamic therapy (PDT). Zinc-MOF can realize acid-responsive release Ce6 improve performance drug destroying integrity bacterial cell membranes enhancing production reactive oxygen species (ROS). Additionally, Ce6@MOF-Gel stability, solubility, properties Ce6. More importantly, inflammation collagen deposition re-epithelialization facilitate healing. Collectively, MOF-based provides new, efficient, safe way for accelerated wounds.

Язык: Английский

Процитировано

71
Nanozyme-Engineered Hydrogels for Anti-Inflammation and Skin Regeneration DOI Creative Commons
Amal George Kurian, Rajendra K. Singh,

Varsha Sagar

и другие.

Nano-Micro Letters, Год журнала: 2024, Номер 16(1)

Опубликована: Фев. 6, 2024

Inflammatory skin disorders can cause chronic scarring and functional impairments, posing a significant burden on patients the healthcare system. Conventional therapies, such as corticosteroids nonsteroidal anti-inflammatory drugs, are limited in efficacy associated with adverse effects. Recently, nanozyme (NZ)-based hydrogels have shown great promise addressing these challenges. NZ-based possess unique therapeutic abilities by combining benefits of redox nanomaterials enzymatic activity water-retaining capacity hydrogels. The multifaceted effects include scavenging reactive oxygen species other inflammatory mediators modulating immune responses toward pro-regenerative environment enhancing regenerative potential triggering cell migration differentiation. This review highlights current state art NZ-engineered (NZ@hydrogels) for regeneration applications. It also discusses underlying chemo-mechano-biological mechanisms behind their effectiveness. Additionally, challenges future directions this ground, particularly clinical translation, addressed. insights provided aid design engineering novel hydrogels, offering new possibilities targeted personalized skin-care therapies.

Язык: Английский

Процитировано

37
Metal natural product complex Ru-procyanidins with quadruple enzymatic activity combat infections from drug-resistant bacteria DOI Creative Commons

Jie Shan,

Xu Jin,

Cong Zhang

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2024, Номер 14(5), С. 2298 - 2316

Опубликована: Янв. 26, 2024

Bacterial infection hampers wound repair by impeding the healing process. Concurrently, inflammation at site triggers production of reactive oxygen species (ROS), causing oxidative stress and damage to proteins cells. This can lead chronic wounds, posing severe risks. Therefore, eliminating bacterial reducing ROS levels are crucial for effective healing. Nanozymes, possessing enzyme-like catalytic activity, convert endogenous substances into highly toxic substances, such as ROS, combat bacteria biofilms without inducing drug resistance. However, current nanozyme model with single enzyme activity falls short meeting complex requirements antimicrobial therapy. Thus, developing nanozymes multiple enzymatic activities is essential. Herein, we engineered a novel metalloenzyme called Ru-procyanidin nanoparticles (Ru-PC NPs) diverse aid infections. Under acidic conditions, due their glutathione (GSH) depletion peroxidase (POD)-like Ru-PC NPs combined H2O2 exhibit excellent antibacterial effects. in neutral environment, NPs, catalase (CAT) decompose O2, alleviating hypoxia ensuring sufficient supply. Furthermore, possess exceptional antioxidant capacity through superior superoxide dismutase (SOD) effectively scavenging excess nitrogen (RNS) environment. maintains balance system prevents inflammation. also promote polarization macrophages from M1 M2, facilitating More importantly, show good biosafety negligible toxicity. In vivo models have confirmed efficacy inhibiting promoting The focus this work highlights quadruple its potential reduce bacteria-infected

Язык: Английский

Процитировано

29
Multifunctional PDA/ZIF8 based hydrogel dressing modulates the microenvironment to accelerate chronic wound healing by ROS scavenging and macrophage polarization DOI
Jinhong Cai, Shuhan Liu,

Qiuling Zhong

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 487, С. 150632 - 150632

Опубликована: Март 24, 2024

Язык: Английский

Процитировано

24
Antibiotics-free nanomaterials against bacterial keratitis: Eliminating infections with reactive oxygen species (ROS) DOI
Liangyu Zhou, Yi Deng, Yujie Ren

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 482, С. 148978 - 148978

Опубликована: Янв. 22, 2024

Язык: Английский

Процитировано

22
Multifunctional hydrogel bioscaffolds based on polysaccharide to promote wound healing: A review DOI
Chuanbo Ding,

Xinglong Liu,

Shuai Zhang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 259, С. 129356 - 129356

Опубликована: Янв. 11, 2024

Язык: Английский

Процитировано

16
Copper‐Based Nanozymes: Potential Therapies for Infectious Wounds DOI Open Access
Haojie Ge, Min Wang,

Xiaolong Wei

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Янв. 5, 2025

Abstract Bacterial infections are a significant obstacle to the healing of acute and chronic wounds, such as diabetic ulcers burn injuries. Traditional antibiotics primary treatment for bacterial infections, but they present issues antibiotic resistance, limited efficacy, potential side effects. This challenge leads exploration nanozymes alternative therapeutic agents. Nanozymes nanomaterials with enzyme‐like activities. Owing their low production costs, high stability, scalability, multifunctionality, have emerged prominent focus in antimicrobial research. Among various types nanozymes, metal‐based offer several benefits, including broad‐spectrum activity robust catalytic properties. Specifically, copper‐based (CuNZs) shown considerable promoting wound healing. They exhibit strong effects, reduce inflammation, enhance tissue regeneration, making them highly advantageous use care. review describes dual functions CuNZs combating infection facilitating repair. Recent advancements design synthesis CuNZs, evaluating promotion, biosafety both vitro vivo on basis core components, critically important.

Язык: Английский

Процитировано

2
Nickel‐Based Metal‐Organic Frameworks Promote Diabetic Wound Healing via Scavenging Reactive Oxygen Species and Enhancing Angiogenesis DOI
Jia Liu,

Zhongyin Chen,

Huan Liu

и другие.

Small, Год журнала: 2023, Номер 20(10)

Опубликована: Ноя. 1, 2023

Abstract Chronic diabetic wounds remain a worldwide challenge for both the clinic and research. Given vicious circle of oxidative stress inflammatory response as well impaired angiogenesis wound tissues, healing process is disturbed poorly responds to current treatments. In this work, nickel‐based metal‐organic framework (MOF, Ni‐HHTP) with excellent antioxidant activity proangiogenic function developed accelerate chronic wounds. The Ni‐HHTP can mimic enzymatic catalytic activities enzymes eliminate multi‐types reactive species through electron transfer reactions, which protects cells from stress‐related damage. Moreover, Ni‐based MOF promote cell migration by activating transforming growth factor‐β1 (TGF‐β1) in vitro reprogram macrophages anti‐inflammatory phenotype. Importantly, effectively promotes suppressing enhancing vivo. This study reports versatile promising MOF‐based nanozyme healing, may be extended combination other dressings enhance management or non‐healing

Язык: Английский

Процитировано

34
Protease-Loaded CuS Nanoparticles with Synergistic Photothermal/Dynamic Therapy against F. nucleatum-Induced Periodontitis DOI
Pengyu Gao, Guotai Li, Zhiguo Wang

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(27), С. 32215 - 32225

Опубликована: Июнь 26, 2023

Periodontitis is a chronic inflammatory disease induced by plaque biofilm, which can lead to the destruction of periodontal support tissue and even teeth loss. The common strategies periodontitis treatment are eliminate bacterial/biofilm-related inflammation subsequently inhibit alveolar bone resorption, for antibiotic therapy most traditional one. However, impenetrable polymeric substances on bacterial biofilms make it difficult antimicrobial agents take effect. In this study, novel nanoparticle protease-loaded CuS NPs was developed, combining advances photodynamic photothermal from enzymatic degradation biofilm protease. activity reactive oxygen generation capacity designed nanoparticles were verified experimental results, constituting basis antibacterial function. Next, high CuS@A onFusobacterium nucleatumand its demonstrated. proper hemo/cytocompatibility CuS-based demonstrated in vitro assays. Last, effective against achieved rat model through significant efficacy inhibiting resorption alleviating inflammation. Thus, developed prove promising material management periodontitis.

Язык: Английский

Процитировано

23