DNA nanotechnology in tumor liquid biopsy: Enrichment and determination of circulating biomarkers
Deleted Journal,
Год журнала:
2023,
Номер
2(1)
Опубликована: Дек. 19, 2023
Abstract
Monitoring
tumor
biomarkers
in
a
non‐invasive
manner
for
diagnosis
has
attracted
increasing
attention.
Liquid
biopsy
mainly
includes
three
types
of
biomarkers:
circulating
cells,
extracellular
vesicles,
and
nucleic
acids.
These
released
by
cells
can
travel
to
other
parts
the
circulation
system.
The
analysis
markers
enables
early
diagnosis,
progression
evolution,
treatment
monitoring,
which
are
important
individualized
clinical
decisions.
In
this
study,
we
summarize
different
DNA
nanotechnology
strategies
that
be
used
capture,
amplify,
measure
discuss
current
challenges
outlook.
Язык: Английский
Applications and Prospects of Micro/nanoelectrodes in Single-cell Imaging and Electrochemical Detection
TrAC Trends in Analytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 118161 - 118161
Опубликована: Янв. 1, 2025
Язык: Английский
The Trend of Nonenzymatic Nucleic Acid Amplification: Strategies and Diagnostic Application
Precision Chemistry,
Год журнала:
2025,
Номер
3(4), С. 187 - 205
Опубликована: Март 3, 2025
Nonenzymatic
nucleic
acid
amplification
reactions,
especially
nonenzymatic
DNA
reactions
(NDARs),
are
thermodynamically
driven
processes
that
operate
without
enzymes,
relying
on
toehold-mediated
strand
displacement
(TMSD)
and
branch
migration.
With
their
sensitive
efficient
signal
capabilities,
NDARs
have
become
essential
tools
for
biomarker
detection
intracellular
imaging.
They
encompass
four
primary
methods:
catalytic
hairpin
assembly
(CHA),
hybridization
chain
reaction
(HCR),
DNAzyme-based
amplification,
entropy-driven
circuits
(EDC).
Based
mechanisms,
can
be
categorized
into
three
types:
stimuli-responsive
NDARs,
which
employ
single
strategies
triggered
by
specific
stimuli
like
pH,
light,
or
biomolecules;
cascade
integrate
two
more
stepwise
enhancement;
autocatalytic
achieve
exponential
through
self-sustained
cycling.
These
advanced
designs
progressively
improve
efficiency,
enhance
sensitivity,
minimize
background
noise,
enabling
precise
of
proteins,
viruses,
acids
as
well
applications
in
cancer
cell
imaging
therapy.
Compared
with
classical
these
approaches
significantly
reduce
leakage,
offering
broader
applicability
diagnostics,
imaging,
therapeutic
contexts.
This
review
summarizes
recent
advancements,
addresses
existing
challenges,
explores
future
directions,
providing
insights
the
development
NDARs.
Язык: Английский
Electrochemical Biosensors for Cancer Diagnosis: Multitarget Analysis to Present Molecular Characteristics of Tumor Heterogeneity
JACS Au,
Год журнала:
2024,
Номер
4(12), С. 4655 - 4672
Опубликована: Дек. 11, 2024
Electrochemical
biosensors
are
gaining
attention
as
powerful
tools
in
cancer
diagnosis,
particularly
liquid
biopsy,
due
to
their
high
efficiency,
rapid
response,
exceptional
sensitivity,
and
specificity.
However,
the
complexity
of
intra-
intertumor
heterogeneity,
with
variations
genetic
protein
expression
profiles
epigenetic
modifications,
makes
electrochemical
susceptible
false-positive
or
false-negative
diagnostic
outcomes.
To
address
this
challenge,
there
is
growing
interest
simultaneously
analyzing
multiple
biomarkers
reveal
molecular
characteristics
tumor
heterogeneity
for
precise
diagnosis.
In
Perspective,
we
highlight
recent
advancements
utilizing
a
specific
emphasis
on
multitarget
analysis
including
tumor-associated
nucleic
acids,
markers,
extracellular
vesicles,
cells.
These
hold
significant
promise
improving
precision
early
diagnosis
monitoring,
well
potentially
offering
new
insights
into
personalized
management.
Язык: Английский
Dual-Targeting PET Tracers Enable Enzyme-Mediated Self-Assembly for the PET Imaging of Legumain Activity
Chunmei Lu,
Ke Li,
Hongjie Xi
и другие.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(38), С. 44654 - 44664
Опубликована: Сен. 13, 2023
Legumain,
a
lysosomal
cysteine
protease
overexpressed
in
variety
of
tumors,
has
been
considered
promising
biomarker
for
various
cancers.
Precise
detection
legumain
activity
the
lysosome
represents
an
important
strategy
early
diagnosis
and
prognosis
tumors.
Small-molecule
probes
with
property
target-enabled
self-assembly
hold
great
potential
molecular
imaging.
In
this
study,
we
reported
two
dual-targeting
radiotracers
([18F]SF-AAN-M
[18F]SF-AAN-HEM)
legumain-mediated
positron
emission
tomography
(PET)
Both
were
synthesized
high
labeling
yield
(>50%)
radiochemical
purity
was
over
99%
via
one-step
straightforward
18F-labeling.
tracers
efficiently
activated
by
reducing
agent
to
self-assemble
into
aggregates
showed
enhanced
retention
legumain-overexpressed
MDA-MB-468
cells
indicating
that
introduction
lysosome-targeting
morpholine
increased
tumor
uptake
extended
addition,
[18F]SF-AAN-HEM
hydrophilic
(histidine-glutamate)3
tag
displayed
significantly
reduced
liver
no
conspicuous
reduction
uptake,
affording
signal-to-noise
ratios
(tumor/liver
tumor/muscle).
All
these
results
suggest
tracer
could
provide
tool
vivo
monitoring
Язык: Английский
Protease-activatable nanoprobe based on peptide decorated Titanium carbide engineering for synchronous monitoring of cancer biomarkers with different spatial distributions
Chemical Engineering Journal,
Год журнала:
2024,
Номер
unknown, С. 157896 - 157896
Опубликована: Ноя. 1, 2024
Язык: Английский
Multispatially Localized DNA Walker Coupling Covalent Organic Framework for Dual-Mode Detection of Nucleocapsid Protein Using the Walking-Recycling-Conversion Strategy
Analytical Chemistry,
Год журнала:
2024,
Номер
96(46), С. 18464 - 18473
Опубликована: Ноя. 6, 2024
DNA
walkers
have
emerged
as
a
powerful
tool
in
bioanalysis;
however,
many
existing
approaches
are
still
restricted
by
low
reaction
kinetics
and
inaccurate
single-mode
detection.
Herein,
fluorescence
(FL)
electrochemical
(EC)
dual-mode
biosensor
was
proposed
based
on
multispatially
localized
walker
(m-DNA
walker)
coupling
covalent
organic
framework
(COF)
using
the
walking-recycling-conversion
strategy.
Specifically,
functionalized
COF
not
only
served
three-dimensional
nanocarrier
but
also
acted
an
effective
quencher
of
walking
tracks.
In
presence
target,
activated
m-DNA
moved
fast
along
numerous
quenching
tracks,
leading
to
cleavage
Cy3-H1
recovery
FL
signal.
To
further
improve
detection
sensitivity,
fragments'
recycling
process
implemented
with
generation
large
amount
S1
S2,
which
caused
assembly
DNA-Fe
Язык: Английский