DNA nanotechnology in tumor liquid biopsy: Enrichment and determination of circulating biomarkers

Deleted Journal, Journal Year: 2023, Volume and Issue: 2(1)

Published: Dec. 19, 2023

Abstract Monitoring tumor biomarkers in a non‐invasive manner for diagnosis has attracted increasing attention. Liquid biopsy mainly includes three types of biomarkers: circulating cells, extracellular vesicles, and nucleic acids. These released by cells can travel to other parts the circulation system. The analysis markers enables early diagnosis, progression evolution, treatment monitoring, which are important individualized clinical decisions. In this study, we summarize different DNA nanotechnology strategies that be used capture, amplify, measure discuss current challenges outlook.

Language: Английский

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Applications and Prospects of Micro/nanoelectrodes in Single-cell Imaging and Electrochemical Detection

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118161 - 118161

Published: Jan. 1, 2025

Language: Английский

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The Trend of Nonenzymatic Nucleic Acid Amplification: Strategies and Diagnostic Application

Precision Chemistry, Journal Year: 2025, Volume and Issue: 3(4), P. 187 - 205

Published: March 3, 2025

Nonenzymatic nucleic acid amplification reactions, especially nonenzymatic DNA reactions (NDARs), are thermodynamically driven processes that operate without enzymes, relying on toehold-mediated strand displacement (TMSD) and branch migration. With their sensitive efficient signal capabilities, NDARs have become essential tools for biomarker detection intracellular imaging. They encompass four primary methods: catalytic hairpin assembly (CHA), hybridization chain reaction (HCR), DNAzyme-based amplification, entropy-driven circuits (EDC). Based mechanisms, can be categorized into three types: stimuli-responsive NDARs, which employ single strategies triggered by specific stimuli like pH, light, or biomolecules; cascade integrate two more stepwise enhancement; autocatalytic achieve exponential through self-sustained cycling. These advanced designs progressively improve efficiency, enhance sensitivity, minimize background noise, enabling precise of proteins, viruses, acids as well applications in cancer cell imaging therapy. Compared with classical these approaches significantly reduce leakage, offering broader applicability diagnostics, imaging, therapeutic contexts. This review summarizes recent advancements, addresses existing challenges, explores future directions, providing insights the development NDARs.

Language: Английский

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Electrochemical Biosensors for Cancer Diagnosis: Multitarget Analysis to Present Molecular Characteristics of Tumor Heterogeneity

JACS Au, Journal Year: 2024, Volume and Issue: 4(12), P. 4655 - 4672

Published: Dec. 11, 2024

Electrochemical biosensors are gaining attention as powerful tools in cancer diagnosis, particularly liquid biopsy, due to their high efficiency, rapid response, exceptional sensitivity, and specificity. However, the complexity of intra- intertumor heterogeneity, with variations genetic protein expression profiles epigenetic modifications, makes electrochemical susceptible false-positive or false-negative diagnostic outcomes. To address this challenge, there is growing interest simultaneously analyzing multiple biomarkers reveal molecular characteristics tumor heterogeneity for precise diagnosis. In Perspective, we highlight recent advancements utilizing a specific emphasis on multitarget analysis including tumor-associated nucleic acids, markers, extracellular vesicles, cells. These hold significant promise improving precision early diagnosis monitoring, well potentially offering new insights into personalized management.

Language: Английский

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Dual-Targeting PET Tracers Enable Enzyme-Mediated Self-Assembly for the PET Imaging of Legumain Activity

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(38), P. 44654 - 44664

Published: Sept. 13, 2023

Legumain, a lysosomal cysteine protease overexpressed in variety of tumors, has been considered promising biomarker for various cancers. Precise detection legumain activity the lysosome represents an important strategy early diagnosis and prognosis tumors. Small-molecule probes with property target-enabled self-assembly hold great potential molecular imaging. In this study, we reported two dual-targeting radiotracers ([18F]SF-AAN-M [18F]SF-AAN-HEM) legumain-mediated positron emission tomography (PET) Both were synthesized high labeling yield (>50%) radiochemical purity was over 99% via one-step straightforward 18F-labeling. tracers efficiently activated by reducing agent to self-assemble into aggregates showed enhanced retention legumain-overexpressed MDA-MB-468 cells indicating that introduction lysosome-targeting morpholine increased tumor uptake extended addition, [18F]SF-AAN-HEM hydrophilic (histidine-glutamate)3 tag displayed significantly reduced liver no conspicuous reduction uptake, affording signal-to-noise ratios (tumor/liver tumor/muscle). All these results suggest tracer could provide tool vivo monitoring

Language: Английский

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Protease-activatable nanoprobe based on peptide decorated Titanium carbide engineering for synchronous monitoring of cancer biomarkers with different spatial distributions

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157896 - 157896

Published: Nov. 1, 2024

Language: Английский

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Multispatially Localized DNA Walker Coupling Covalent Organic Framework for Dual-Mode Detection of Nucleocapsid Protein Using the Walking-Recycling-Conversion Strategy

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(46), P. 18464 - 18473

Published: Nov. 6, 2024

DNA walkers have emerged as a powerful tool in bioanalysis; however, many existing approaches are still restricted by low reaction kinetics and inaccurate single-mode detection. Herein, fluorescence (FL) electrochemical (EC) dual-mode biosensor was proposed based on multispatially localized walker (m-DNA walker) coupling covalent organic framework (COF) using the walking-recycling-conversion strategy. Specifically, functionalized COF not only served three-dimensional nanocarrier but also acted an effective quencher of walking tracks. In presence target, activated m-DNA moved fast along numerous quenching tracks, leading to cleavage Cy3-H1 recovery FL signal. To further improve detection sensitivity, fragments' recycling process implemented with generation large amount S1 S2, which caused assembly DNA-Fe

Language: Английский

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