ACS Sensors,
Год журнала:
2021,
Номер
6(7), С. 2497 - 2522
Опубликована: Июнь 18, 2021
Clustered
regularly
interspaced
short
palindromic
repeats,
CRISPR,
has
recently
emerged
as
a
powerful
molecular
biosensing
tool
for
nucleic
acids
and
other
biomarkers
due
to
its
unique
properties
such
collateral
cleavage
nature,
room
temperature
reaction
conditions,
high
target-recognition
specificity.
Numerous
platforms
have
been
developed
leverage
the
CRISPR
assay
ultrasensitive
applications.
However,
be
considered
new
gold
standard,
several
key
challenges
must
addressed.
In
this
paper,
we
briefly
review
history
of
biosensors,
followed
by
current
status
acid-based
detection
methods.
We
then
discuss
pertaining
CRISPR-based
acid
detection,
recent
breakthroughs
addressing
these
challenges.
focus
upon
future
advancements
required
enable
rapid,
simple,
sensitive,
specific,
multiplexed,
amplification-free,
shelf-stable
biosensors.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2022,
Номер
21(4), С. 3770 - 3798
Опубликована: Июль 1, 2022
Abstract
Food
safety
is
one
of
the
biggest
public
issues
occurring
around
world.
Microbiological,
chemical,
and
physical
hazards
can
lead
to
food
issues,
which
may
occur
at
all
stages
supply
chain.
In
order
tackle
safeguard
consumer
health,
rapid,
accurate,
specific,
field‐deployable
detection
methods
meeting
diverse
requirements
are
imperative
measures
for
assurance.
CRISPR‐Cas
system,
a
newly
emerging
technology,
has
been
successfully
repurposed
in
biosensing
demonstrated
huge
potential
establish
conceptually
novel
with
high
sensitivity
specificity.
This
review
focuses
on
CRISPR‐Cas–based
its
current
status
specifically
inspection.
We
firstly
illustrate
pending
problems
summarize
popular
methods.
then
describe
applications
Finally,
challenges
futuristic
opportunities
proposed
discussed.
Generally
speaking,
still
unsatisfactory
some
ways
such
as
being
time‐consuming,
displaying
unmet
specificity
standards,
there
comparative
paucity
multiplexed
testing
POCT.
Recent
studies
have
shown
that
an
innovative
fast‐expanding
could
make
up
shortcomings
existing
or
even
replace
them.
To
sum
up,
implementation
integration
other
techniques
promising
desirable,
expected
provide
“customized”
“smart”
inspection
coming
future.
Development
of
a
multiplexed
and
sensitive
biosensing
platform
is
priority
for
public
health
security.
We
report
micropore
resistance
counting
based
on
polystyrene
microsphere
size-based
encoding
Clostridium
butyricum
Argonaute
(CbAgo)
decoding
ultrasensitive
detection.
Initially,
we
constructed
target
DNA-modified
coding
system
counting.
Subsequently,
the
precise
recognition
cleavage
capabilities
guide
DNA-activated
CbAgo
protein
enable
encoded
system.
Changes
in
concentration
microspheres
are
presented
as
signal
readout.
The
demonstrated
excellent
performance
detection
three
mycotoxins
(with
sensitivity
range
over
4
orders
magnitude
reaching
pg/mL
level)
two
inflammatory
markers
at
pg/mL.
Combining
enzyme
by
with
counting,
developed
highly
tool
wide-ranging
potential
applications
such
clinical
diagnosis,
food
safety
inspection,
environmental
monitoring.
ACS Nano,
Год журнала:
2021,
Номер
15(5), С. 7848 - 7859
Опубликована: Май 7, 2021
Clustered,
regularly
interspaced
short
palindromic
repeats
(CRISPR)-based
diagnoses,
derived
from
gene-editing
technology,
have
been
exploited
for
less
than
5
years
and
are
now
reaching
the
stage
of
precommercial
use.
CRISPR
tools
some
notable
features,
such
as
recognition
at
physiological
temperature,
excellent
specificity,
high-efficiency
signal
amplification
capabilities.
These
characteristics
promising
development
next-generation
diagnostic
technologies.
In
this
Perspective,
we
present
a
detailed
summary
which
micro/nanotechnologies
play
roles
in
advancement
diagnosis
how
they
involved.
The
use
nanoprobes,
nanochips,
nanodevices,
microfluidic
lateral
flow
strips,
etc.
detection
systems
has
led
to
new
opportunities
CRISPR-based
assay
development,
achieving
equipment-free
detection,
providing
more
compact
systems,
improving
sensitivity
quantitative
Although
tremendous
progress
made,
not
yet
reached
its
full
potential.
We
discuss
upcoming
improvements
will
continue
key
roles.
