Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 215, P. 114559 - 114559

Published: July 13, 2022

Language: Английский

---

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

Analytica Chimica Acta, Journal Year: 2021, Volume and Issue: 1185, P. 338882 - 338882

Published: July 26, 2021

Language: Английский

---

CRISPR/Cas-powered nanobiosensors for diagnostics

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 197, P. 113732 - 113732

Published: Oct. 29, 2021

Language: Английский

---

CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2022, Volume and Issue: 21(4), P. 3770 - 3798

Published: July 1, 2022

Abstract Food safety is one of the biggest public issues occurring around world. Microbiological, chemical, and physical hazards can lead to food issues, which may occur at all stages supply chain. In order tackle safeguard consumer health, rapid, accurate, specific, field‐deployable detection methods meeting diverse requirements are imperative measures for assurance. CRISPR‐Cas system, a newly emerging technology, has been successfully repurposed in biosensing demonstrated huge potential establish conceptually novel with high sensitivity specificity. This review focuses on CRISPR‐Cas–based its current status specifically inspection. We firstly illustrate pending problems summarize popular methods. then describe applications Finally, challenges futuristic opportunities proposed discussed. Generally speaking, still unsatisfactory some ways such as being time‐consuming, displaying unmet specificity standards, there comparative paucity multiplexed testing POCT. Recent studies have shown that an innovative fast‐expanding could make up shortcomings existing or even replace them. To sum up, implementation integration other techniques promising desirable, expected provide “customized” “smart” inspection coming future.

Language: Английский

---

Biosensors for healthcare: current and future perspectives

Trends in biotechnology, Journal Year: 2022, Volume and Issue: 41(3), P. 374 - 395

Published: Dec. 23, 2022

Language: Английский

---

Functional DNA sensors integrated with nucleic acid signal amplification strategies for non-nucleic acid targets detection

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 230, P. 115282 - 115282

Published: April 1, 2023

Language: Английский

---

Micropore Resistance Counting Platform for Multiplexed and Ultrasensitive Detection of Mycotoxins and Biomarkers

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Development of a multiplexed and sensitive biosensing platform is priority for public health security. We report micropore resistance counting based on polystyrene microsphere size-based encoding Clostridium butyricum Argonaute (CbAgo) decoding ultrasensitive detection. Initially, we constructed target DNA-modified coding system counting. Subsequently, the precise recognition cleavage capabilities guide DNA-activated CbAgo protein enable encoded system. Changes in concentration microspheres are presented as signal readout. The demonstrated excellent performance detection three mycotoxins (with sensitivity range over 4 orders magnitude reaching pg/mL level) two inflammatory markers at pg/mL. Combining enzyme by with counting, developed highly tool wide-ranging potential applications such clinical diagnosis, food safety inspection, environmental monitoring.

Language: Английский

---

Programmable DNA Nanoswitch-Regulated Plasmonic CRISPR/Cas12a-Gold Nanostars Reporter Platform for Nucleic Acid and Non-Nucleic Acid Biomarker Analysis Assisted by a Spatial Confinement Effect

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

CRISPR/Cas 12a system based nucleic acid and non-nucleic targets detection faces two challenges including (1) multiple crRNAs are needed for biomarkers (2) insufficient sensitivity resulted from photobleaching of fluorescent dyes the low kinetic cleavage rate a traditional single-strand (ssDNA) reporter. To address these limitations, we developed programmable DNA nanoswitch (NS)-regulated plasmonic CRISPR/Cas12a-gold nanostars (Au NSTs) reporter platform with assistance spatial confinement effect. Through simply programming target recognition sequence in NS, only one crRNA is required to detect both biomarkers. The limit decreased by ∼196-fold miRNA-375 122-fold prostate-specific antigen (PSA), respectively. Moreover, versatile evaluation PSA clinical urine samples can also be achieved, according which prostate cancer healthy groups well identified.

Language: Английский

---

Advances in nanomaterial-based microfluidic platforms for on-site detection of foodborne bacteria

TrAC Trends in Analytical Chemistry, Journal Year: 2021, Volume and Issue: 147, P. 116509 - 116509

Published: Dec. 10, 2021

Language: Английский

---

Advances in Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)-Based Diagnostic Assays Assisted by Micro/Nanotechnologies

ACS Nano, Journal Year: 2021, Volume and Issue: 15(5), P. 7848 - 7859

Published: May 7, 2021

Clustered, regularly interspaced short palindromic repeats (CRISPR)-based diagnoses, derived from gene-editing technology, have been exploited for less than 5 years and are now reaching the stage of precommercial use. CRISPR tools some notable features, such as recognition at physiological temperature, excellent specificity, high-efficiency signal amplification capabilities. These characteristics promising development next-generation diagnostic technologies. In this Perspective, we present a detailed summary which micro/nanotechnologies play roles in advancement diagnosis how they involved. The use nanoprobes, nanochips, nanodevices, microfluidic lateral flow strips, etc. detection systems has led to new opportunities CRISPR-based assay development, achieving equipment-free detection, providing more compact systems, improving sensitivity quantitative Although tremendous progress made, not yet reached its full potential. We discuss upcoming improvements will continue key roles.

Language: Английский

---