ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(34), С. 44386 - 44398
Опубликована: Авг. 16, 2024
Extracellular
vesicles
(EVs)
are
present
in
blood
at
much
lower
concentrations
(5-6
orders
of
magnitude)
compared
to
lipoprotein
particles
(LP).
Because
LP
and
EV
overlap
size
density,
isolating
high-purity
EVs
is
a
significant
challenge.
While
the
current
two-step
sequential
isolation
process
using
size-expression
chromatography
(SEC)
followed
by
density
gradient
(DG)
achieves
high
purity,
time-consuming
ultracentrifugation
(UC)
step
DG
hinders
workflow
efficiency.
This
paper
introduces
an
optimized
magnetic
bead
reagent,
LipoMin,
functionalized
with
glycosaminoglycans
(GAGs),
as
rapid
alternative
for
removal
during
second-step
about
10
minutes.
We
evaluated
LipoMin's
efficacy
on
two
sample
types:
(a)
fractions
isolated
exclusion
(SEC
+
LipoMin)
(b)
pellet
obtained
from
(UC
LipoMin).
The
remarkably
simple,
involving
min
incubation
LipoMin
separation
LP-depleted
EV-containing
supernatant.
Results
enzyme-linked
immunosorbent
assay
(ELISA)
revealed
that
removes
98.2%
ApoB
SEC
fractions,
comparable
ability
process.
Importantly,
yield
(CD81
ELISA)
remained
93.0%
Western
blot
analysis
confirmed
key
markers,
flotillin
CD81,
were
not
compromised.
Recombinant
(rEV),
reference
standard,
was
spiked
into
recovered
89%
CD81
protein.
For
UC
ApoA1
decreased
76.5%
while
retaining
90.7%
CD81.
Notably,
both
colorectal
cancer
(CRC)
Alzheimer's
disease
(AD)
samples
processed
displayed
clear
expression
relevant
clinical
markers.
With
(resulting
96%
time
saving
traditional
method),
reagent
offers
efficient
depletion,
paving
way
streamlined
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(17)
Опубликована: Янв. 30, 2023
Abstract
Polymerase
chain
reaction
(PCR)‐based
nucleic
acid
testing
has
played
a
critical
role
in
disease
diagnostics,
pathogen
surveillance,
and
many
more.
However,
this
method
requires
long
turnaround
time,
expensive
equipment,
trained
personnel,
limiting
its
widespread
availability
diagnostic
capacity.
On
the
other
hand,
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
technology
recently
demonstrated
capability
for
detection
with
high
sensitivity
specificity.
CRISPR‐mediated
biosensing
holds
great
promise
revolutionizing
procedures
developing
point‐of‐care
diagnostics.
This
review
focuses
on
recent
developments
both
fundamental
CRISPR
biochemistry
CRISPR‐based
techniques.
Four
ongoing
research
hotspots
molecular
diagnostics‐target
preamplification‐free
detection,
microRNA
(miRNA)
testing,
non‐nucleic‐acid
SARS‐CoV‐2
detection‐are
also
covered.
ACS Nano,
Год журнала:
2024,
Номер
18(14), С. 9784 - 9797
Опубликована: Март 12, 2024
Extracellular
vesicles
(EVs)
secreted
by
all
cell
types
are
involved
in
the
cell-to-cell
transfer
of
regulatory
factors
that
influence
and
tissue
phenotypes
normal
diseased
tissues.
EVs
thus
a
rich
source
biomarker
targets
for
assays
analyze
blood
urinary
disease
diagnosis.
Sensitive
detection
derived
from
specific
populations
is
key
major
hurdle
when
analyzing
complex
biological
samples,
but
innovative
approaches
surveyed
this
Perspective
can
streamline
EV
isolation
enhance
sensitivity
procedures
required
clinical
application
EV-based
diagnostics
therapeutics,
including
nanotechnology
microfluidics,
to
achieve
characterizations.
Finally,
also
outlines
opportunities
challenges
remaining
translation
assays.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(9), С. 2236 - 2252
Опубликована: Янв. 1, 2024
Plant-derived
exosomes
(PDEs)
show
promising
potential
to
be
developed
as
a
therapeutic
agent
against
cancer,
owing
their
multiple
advantages
such
low
toxicity,
biocompatibility,
availability,
affordability,
etc.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(29), С. 11019 - 11027
Опубликована: Июль 7, 2023
A
microdroplet-based
surface-enhanced
Raman
spectroscopy
(microdroplet
SERS)
platform
was
constructed
to
envelop
individual
cells
in
microdroplets,
followed
by
the
SERS
detection
of
their
extracellular
vesicle-proteins
(EV-proteins)
via
in-drop
immunoassays
use
immunomagnetic
beads
(iMBs)
and
immuno-SERS
tags
(iSERS
tags).
unique
phenomenon
is
found
that
iMBs
can
start
a
spontaneous
reorientation
on
probed
cell
surface
based
electrostatic
force-driven
interfacial
aggregation
effect,
which
leads
EV-proteins
iSERS
be
gathered
from
liquid
phase
membrane
interface
significantly
improves
sensitivity
single-cell
analysis
level
due
formation
numbers
hotspots.
Three
two
breast
cancer
lines
were
collected
further
analyzed
machine
learning
algorithmic
tools,
will
helpful
for
deeper
understanding
subtypes
view
EV-proteins.
ACS Applied Bio Materials,
Год журнала:
2023,
Номер
6(7), С. 2576 - 2590
Опубликована: Июнь 14, 2023
Cancer
is
a
complex
deadly
disease
that
has
caused
global
health
crisis
in
recent
epochs.
Colorectal
cancer
(CRC)
the
third
most
common
malignant
gastrointestinal
disease.
It
led
to
high
mortality
due
early
diagnostic
failure.
Extracellular
vesicles
(EVs)
come
with
promising
solutions
for
CRC.
Exosomes
(a
subpopulation
of
EVs)
play
vital
role
as
signaling
molecules
CRC
tumor
microenvironment.
secreted
from
all
active
cells.
Exosome-based
molecular
transport
(DNA,
RNA,
proteins,
lipids,
etc.)
transforms
recipient
cell's
nature.
In
CRC,
cell-derived
exosomes
(TEXs)
regulate
multiple
events
development
and
progression
such
immunogenic
suppression,
angiogenesis,
epithelial–mesenchymal
transitions
(EMT),
physical
changes
extracellular
matrix
(ECM),
metastasis.
Biofluid-circulated
tumor-derived
are
potential
tool
liquid
biopsy.
colorectal
detection
creates
great
impact
biomarker
research.
The
exosome-associated
theranostics
approach
state-of-the-art
method.
this
review,
we
address
associated
progression,
on
screening
(diagnostic
prognostic
biomarkers),
also
highlight
several
clinical
trials,
well
future
directions
exosome-based
Hopefully,
it
will
encourage
researchers
develop
theranostic
fight
The Journal of Liquid Biopsy,
Год журнала:
2023,
Номер
2, С. 100129 - 100129
Опубликована: Ноя. 26, 2023
Cancer
is
an
unsolved
health
crisis
worldwide.
Extracellular
vesicles
(EVs)
address
this
problem
in
a
new
way.
In
cancer,
early
detection
highly
challenging,
exosomes
(a
subpopulation
of
EVs,
originating
from
endosomes)
overcome
limitation.
tumor-derived
(TEXs)
play
role
as
signaling
molecules
cancer
development
and
progression.
TEXs
required
more
detailed
investigation
for
specific
biomarkers
research.
Exosome
heterogeneity
(some
the
facts
regulate
it
such
exosome
size,
origin,
inner
molecular
diversity)
made
complication
liquid
biopsy.
Single
profiling
barcoding
support
overcoming
limitation
developing
precision
oncology.
interdisciplinary
approach
screening
specifically.
Journal of Cellular and Molecular Medicine,
Год журнала:
2023,
Номер
27(11), С. 1603 - 1607
Опубликована: Май 15, 2023
This
short
communication
will
enlighten
the
readers
about
exosome
and
epithelial-mesenchymal
transition
(EMT)
related
to
several
complicated
events.
It
also
highlighted
therapeutic
potential
of
exosomes
against
EMT.
Exosome
toxicology,
heterogeneity,
a
single
profiling
approach
are
covered
in
this
article.
In
future,
could
help
us
get
closer
cancer
vaccine
precision
oncology.
