Novel Ratiometric Surface-Enhanced Raman Scattering (SERS) Biosensor for Ultrasensitive Quantitative Monitoring of Human Carboxylesterase-1 in Hepatocellular Carcinoma Cells Using Ag–Au Nanoflowers as SERS Substrate

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(46), P. 18555 - 18563

Published: Nov. 5, 2024

In this study, we developed ratiometric surface-enhanced Raman scattering (SERS) biosensors using Ag-Au alloy nanoflowers as SERS substrates, molecules having amide bonds and alkyne groups (Tag A) reporters, sodium thiocyanate an internal standard molecule B) for the sensitive detection of human carboxylesterase-1 (hCE1) in HepG-2 cells. The correlation between cell damage hCE1 activity levels was investigated. Both Tag A's group B's cyanide produced characteristic signals Raman-silent region (

Language: Английский

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Recent advancements in the applications of separation and enrichment strategies towards SERS detection

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 184, P. 118136 - 118136

Published: Jan. 7, 2025

Language: Английский

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Fully Integrated and High‐Throughput Microfluidic System for Multiplexed Point‐Of‐Care Testing

Small, Journal Year: 2024, Volume and Issue: 20(43)

Published: June 28, 2024

Abstract For every epidemic outbreak, the prevention and treatments in resource‐limited areas are always out of reach. Critical to this is that high accuracy, stability, more comprehensive analytical techniques rely on expensive bulky instruments large laboratories. Here, a fully integrated high‐throughput microfluidic system proposed for ultra‐multiple point‐of‐care immunoassay, termed Dac system. Specifically, only requires handheld portable device automatically recycle repetitive multi‐step reactions including on‐demand liquid releasing, dispensing, metering, collecting, oscillatory mixing, discharging. The performs high‐precision enzyme‐linked immunosorbent assays up 17 samples or targets simultaneously single chip. Furthermore, reagent consumption 2% compared conventional ELISA, microbubble‐accelerated shorten assay time by than half. As proof concept, multiplexed detections achieved detecting at least four infection two singular barcode‐based multi‐target results can rapidly distinguish between five similar cases, allowing accurate therapeutic interventions. Compared clinical instruments, accuracy inflammation classification 92.38% ( n = 105), with quantitative correlation coefficient R 2 0.9838, while specificity 100% sensitivity 98.93%.

Language: Английский

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DNA‐encoded plasmonic bubbles aggregating dual‐microRNA SERS signals for cancer diagnosis

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Abstract Solid bubbles have expanded the SERS assay toolbox, but their detection performance in biofluids is still hampered by irrational design of plasmonic sensing interface. A bubble aggregate‐driven DNA‐encoded reported here that enables simultaneous, ultrasensitive, and specific multiple miRNAs blood samples for accurate cancer diagnosis. In this assay, buoyancy allows them to self‐aggregate at a droplet apex reconfiguration, form single‐layer aggregates with nanogaps, prevent coffee ring effect during evaporation assembly. Furthermore, seamlessly couple dual‐color catalytic hybridization assembly amplify miRNA‐responsive Raman signal, function as both an analyte concentrator signal aggregator without external forces. Using these merits, magnet‐free, portable achieves femtomolar dual‐miRNA quantitation single‐base resolution, simultaneous miRNA across four cell lines, diagnosis (AUC = 1) via analyzing 40 machine learning, thus providing promising tool clinical

Language: Английский

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One-Step Capture and Profiling of Single Exosomes from Whole Blood Using Nanocavities through a Cascade Capture Strategy

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137315 - 137315

Published: Jan. 1, 2025

Language: Английский

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Electric Field-Resistant Bubble-Enhanced Wash-Free Profiling of Extracellular Vesicle Surface Markers

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

Efficient profiling of circulating extracellular vesicles (EVs) benefits noninvasive cancer diagnosis and therapeutic monitoring, but is technically hampered by tedious isolation, multistep washing, poor sensitivity. Here, we report multifunctional bubbles that enable self-separation, wash-free, single-step, ultrasensitive EV surface markers in plasma samples for early treatment monitoring lung cancer. In this assay, the buoyancy-dominated bubble electric field-resistant, allowing EV-responsive release electroactive probes electrohydrodynamic nanoshearing force-enhanced hybridization, self-separation from electrode interface minimizing noise electrochemical measurements, one-step wash-free profiling. This assay achieves sensitivity near a single-EV level, shows high specificity against nontarget EVs, tracks phenotypic changes induced drugs. We further show technology can classify (n = 111) between patients noncancer controls with accuracies >95%, accurate via machine learning, monitor pre/post-surgery efficacy higher accuracy over routine clinical serum markers. bubble-driven provides promising quantitative tool to precision liquid biopsies.

Language: Английский

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Multifunctional Fe3O4@ZIF-8@Ag nanocomposites and electro-driven droplet adsorption strategy for SERS detection of acetamiprid

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161149 - 161149

Published: Feb. 1, 2025

Language: Английский

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Nanostructured Bubbles‐Enhanced Fluorescence for Ultrasensitive Portable MicroRNA Detection

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract The unmet on‐site clinical diagnostics are increasingly demanding portable bioassays, but compromised by sensitivity and throughput. Herein, a nanostructured bubbles‐enhanced fluorescence (nBEF) assay is reported interfacing with smartphone imaging system to enable portable, ultrasensitive, specific miRNA detection in parallel. In nBEF, the high‐curvature bubbles allow physically enhancing optical propagation, chemically fluorescence, assembling more DNA probes, undergoing faster self‐suspension efficient self‐aggregation enhancement relative their smooth counterparts. By coupling bubble‐driven signal amplification plug‐and‐play concatemer, system, this nBEF achieves ultrasensitive fluorescent of at femtomolar level one‐base resolution single test, capable monitoring drug‐induced dynamic expression accurate cancer diagnosis (AUC = 1) through profiling 40 plasma samples after RNA extraction. This further integrates an R‐value analysis RGB module realize automated, readout. Such high‐performance provides low‐cost, highly sensitive tool diagnosis.

Language: Английский

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A microfluidic-based chemiluminescence biosensor for sensitive multiplex detection of exosomal microRNAs based on hybridization chain reaction

Talanta, Journal Year: 2024, Volume and Issue: 281, P. 126838 - 126838

Published: Sept. 7, 2024

Language: Английский

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Au/Ag@ZIF-8 nanocomposite as solid phase extraction adsorbent and SERS substrate for tacrolimus label-free therapeutic drug monitoring in human serum

Talanta, Journal Year: 2024, Volume and Issue: 281, P. 126813 - 126813

Published: Sept. 5, 2024

Language: Английский

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