International Journal of Extreme Manufacturing,
Год журнала:
2023,
Номер
6(1), С. 012006 - 012006
Опубликована: Сен. 25, 2023
Abstract
Adequate
vascularization
is
a
critical
determinant
for
the
successful
construction
and
clinical
implementation
of
complex
organotypic
tissue
models.
Currently,
low
cell
vessel
density
insufficient
vascular
maturation
make
vascularized
difficult,
greatly
limiting
its
use
in
engineering
regenerative
medicine.
To
address
these
limitations,
recent
studies
have
adopted
pre-vascularized
microtissue
assembly
rapid
generation
functional
analogs
with
dense
networks
high
density.
In
this
article,
we
summarize
development
module
assembly-based
application
repair
regeneration,
organ-scale
biomanufacturing,
as
well
advanced
modeling.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(21), С. 13116 - 13116
Опубликована: Окт. 28, 2022
The
central
nervous
system
(CNS)
controls
and
regulates
the
functional
activities
of
organ
systems
maintains
unity
between
body
external
environment.
advent
co-culture
has
made
it
possible
to
elucidate
interactions
neural
cells
in
vitro
reproduce
complex
circuits.
Here,
we
classified
as
a
two-dimensional
(2D)
system,
cell-based
three-dimensional
(3D)
tissue
slice-based
3D
an
organoid-based
microfluidic
platform-based
system.
We
provide
overview
these
different
models
their
applications
study
cell
interaction.
application
virus-infected
CNS
disease
is
also
discussed
here.
Finally,
direction
future
research
prospected.
ACS Sensors,
Год журнала:
2024,
Номер
9(7), С. 3466 - 3488
Опубликована: Июль 11, 2024
Organ-on-a-Chip
(OOC)
technology,
which
emulates
the
physiological
environment
and
functionality
of
human
organs
on
a
microfluidic
chip,
is
undergoing
significant
technological
advancements.
Despite
its
rapid
evolution,
this
technology
also
facing
notable
challenges,
such
as
lack
vascularization,
development
multiorgan-on-a-chip
systems,
replication
body
single
chip.
The
progress
has
played
crucial
role
in
steering
OOC
toward
mimicking
microenvironment,
including
microenvironment
replication,
multiorgan
microphysiological
systems.
Additionally,
advancements
detection,
analysis,
organoid
imaging
technologies
have
enhanced
efficiency
Organs-on-Chips
(OOCs).
In
particular,
integration
artificial
intelligence
revolutionized
imaging,
significantly
enhancing
high-throughput
drug
screening.
Consequently,
review
covers
research
Human-on-a-chip,
sensors
OOCs,
latest
applications
biomedical
field.
ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
10(5), С. 3203 - 3217
Опубликована: Апрель 1, 2024
The
intricate
electrophysiological
functions
and
anatomical
structures
of
spinal
cord
tissue
render
the
establishment
in
vitro
models
for
cord-related
diseases
highly
challenging.
Currently,
both
vivo
are
still
underdeveloped,
complicating
exploration
development
effective
therapeutic
drugs
or
strategies.
Organoids
cultured
from
human
induced
pluripotent
stem
cells
(hiPSCs)
hold
promise
as
suitable
diseases.
However,
cultivation
organoids
predominantly
relies
on
Matrigel,
a
matrix
derived
murine
sarcoma
tissue.
Tissue-specific
extracellular
matrices
key
drivers
complex
organ
development,
thus
underscoring
urgent
need
to
research
safer
more
physiologically
relevant
organoid
culture
materials.
Herein,
we
have
prepared
rat
decellularized
brain
hydrogel
(DBECMH),
which
supports
formation
hiPSC-derived
organoids.
Compared
with
DBECMH
exhibited
higher
expression
levels
markers
multiple
compartments
natural
cord,
facilitating
maturation
tissues.
Our
study
suggests
that
holds
potential
replace
Matrigel
standard
medium
organoids,
thereby
advancing
protocols
their
application
modeling
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(10), С. 5788 - 5788
Опубликована: Май 21, 2022
Three-dimensional
(3D)
cultures,
so-called
organoids,
have
emerged
as
an
attractive
tool
for
disease
modeling
and
therapeutic
innovations.
Here,
we
aim
to
determine
if
boundary
cap
neural
crest
stem
cells
(BC)
can
survive
differentiate
in
gelatin-based
3D
bioprinted
bioink
scaffolds
order
establish
enabling
technology
the
fabrication
of
spinal
cord
organoids
on
a
chip.
BC
previously
demonstrated
ability
support
survival
differentiation
co-implanted
or
co-cultured
supported
motor
neuron
excitotoxically
challenged
slice
cultures.
We
tested
different
combinations
cross-linked
material,
analyzed
surface
inside
scaffolds,
then
human
iPSC-derived
(motor
precursors
astrocytes)
be
printed
with
same
protocol,
which
was
developed
BC.
showed
that
this
protocol
is
applicable
cells.
Neural
more
prominent
peripheral
compared
central
parts
construct,
presumably
because
easier
access
differentiation-promoting
factors
medium.
These
findings
show
enzymatically
hydrogel
suitable
building
multicellular,
organoid,
but
further
measures
are
still
required
achieve
uniform
differentiation.
ECS Sensors Plus,
Год журнала:
2023,
Номер
2(4), С. 042401 - 042401
Опубликована: Дек. 1, 2023
Electrochemical,
aptamer-based
(E-AB)
sensors
provide
a
generalizable
strategy
to
quantitatively
detect
variety
of
targets
including
small
molecules
and
proteins.
The
key
signaling
attributes
E-AB
(sensitivity,
selectivity,
specificity,
reagentless
dynamic
sensing
ability)
make
them
well
suited
monitor
processes
in
complex
environments.
A
bioanalytical
challenge
that
could
benefit
from
the
detection
capabilities
is
cell
signaling,
which
involves
release
molecular
messengers
into
extracellular
space.
Here,
we
perspective
on
why
are
for
this
measurement,
sensor
requirements,
pioneering
examples
cellular
measurements.
Abstract
Central
Nervous
System
(CNS)
disorders
represent
a
profound
public
health
challenge
that
affects
millions
of
people
around
the
world.
Diseases
such
as
Alzheimer’s
disease
(AD),
Parkinson’s
(PD),
and
traumatic
brain
injury
(TBI)
exemplify
complexities
diversities
complicate
their
early
detection
development
effective
treatments.
Amid
these
challenges,
emergence
nanotechnology
extracellular
vesicles
(EVs)
signals
new
dawn
for
treating
diagnosing
CNS
ailments.
EVs
are
cellularly
derived
lipid
bilayer
nanosized
particles
pivotal
in
intercellular
communication
within
have
potential
to
revolutionize
targeted
therapeutic
delivery
identification
novel
biomarkers.
Integrating
with
amplifies
diagnostic
capabilities,
opening
avenues
managing
diseases.
This
review
focuses
on
examining
fascinating
interplay
between
theranostics.
Through
highlighting
remarkable
advancements
unique
methodologies,
we
aim
offer
valuable
perspectives
how
approaches
can
bring
about
revolutionary
change
management.
The
objective
is
harness
distinctive
attributes
forge
personalized,
efficient
interventions
disorders,
thereby
providing
beacon
hope
affected
individuals.
In
short,
confluence
heralds
promising
frontier
impactful
treatments
against
diseases,
which
continue
pose
significant
challenges.
By
focusing
personalized
powerful
methods,
might
improve
quality
patients.
Advanced NanoBiomed Research,
Год журнала:
2022,
Номер
2(12)
Опубликована: Ноя. 20, 2022
Organoids
refer
to
3D
stem
cells
that
have
been
developed
model
neurological
disorders
in
vitro.
Typically,
brain
and
heart
organoids
gained
interest
for
their
potential
truly
mimic
the
functional
ability
of
real
organs.
Morphological
analysis
methods
using
immunostaining
slicing
are
explored
extensively
over
past
decade
evaluate
structures
functions
organoids.
However,
destructiveness
these
limits
real‐time
monitoring
dynamic
responses
Therefore,
electrophysiological
with
minimally
invasive
forms
can
be
a
key
solution
an
improved
understanding
nature
complex
Herein,
latest
advances
recording
platforms
reviewed
comprehensive
study
considerations
regarding
factors
is
provided.
Furthermore,
current
challenges
discussed
along
prospects
next‐generation
Abstract
Biohybrid
robots
have
been
developed
for
biomedical
applications
and
industrial
robotics.
However,
the
biohybrid
limitations
to
be
applied
in
neurodegenerative
disease
research
due
absence
of
a
central
nervous
system.
In
addition,
organoids‐on‐a‐chip
has
not
yet
able
replicate
physiological
function
muscle
movement
human
motor
system,
which
is
essential
evaluating
accuracy
drugs
used
treating
diseases.
Here,
system‐based
robot‐on‐a‐chip
composed
brain
organoid,
multi‐motor
neuron
spheroids,
bundle
on
solid
substrateis
proposed
evaluate
drug
effect
diseases
first
time.
The
electrophysiological
signals
from
cerebral
organoid
induced
through
spheroids.
To
Parkinson's
(PD),
patient‐derived
midbrain
generated
incorporated
into
robot‐on‐a‐chip.
PD
successfully
evaluated
by
measuring
movement.
organoid‐based
increased
4.5
±
0.99
µm
18.67
2.25
response
levodopa.
can
serve
as
standard
robot
model
evaluation.
Advanced NanoBiomed Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 23, 2024
Though
animal
models
are
still
the
gold
standard
for
fundamental
biological
studies
and
drug
evaluation
brain
diseases,
concerns
arise
from
an
apparent
lack
of
reflecting
human
genetics
pathophysiology.
Recently,
avatars
such
as
brain‐on‐a‐chip
organoids
which
generated
in
a
3D
manner
using
multiple
types
human‐originated
cells
have
risen
alternative
testing
models.
Particularly
monitoring
functional
neuronal
that
express
action
potentials
or
organoids,
various
methods
measuring
their
electrophysiological
function
been
suggested
study
brain‐related
disease.
Recent
methodologies
analyzing
electrophysiology
different
summarized
this
review.
We
first
emphasize
inherent
features
perspective
cell
culture
environment
accessibility
to
deep
layer.
The
applicable
techniques
then
overviewed
based
on
these
features.
Finally,
we
discuss
unmet
needs
advanced
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