ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(37), С. 49218 - 49226
Опубликована: Сен. 6, 2024
As
an
important
biomarker,
tumor
cell-derived
exosomes
have
substantial
application
prospects
in
early
cancer
screening
and
diagnosis.
However,
the
unsatisfactory
sensitivity
complicated
sample
pretreatment
processes
of
conventional
detection
approaches
limited
their
use
clinical
Nanopore
sensors,
as
a
highly
sensitive,
label-free,
single-molecule
technology,
are
widely
utilized
molecule
bioparticle
detection.
Nevertheless,
exosome
capture
rate
through
nanopores
is
extremely
low
due
to
surface
charge
densities
effects
electrolyte
concentration
on
structural
stability,
thereby
reducing
throughput.
Here,
we
report
approach
improve
translocations
using
silicon
nitride
(SiN
Abstract
Single‐cell
multiomic
and
exosome
analyses
are
potent
tools
in
various
fields,
such
as
cancer
research,
immunology,
neuroscience,
microbiology,
drug
development.
They
facilitate
the
in‐depth
exploration
of
biological
systems,
providing
insights
into
disease
mechanisms
aiding
treatment.
isolation,
which
is
crucial
for
single‐cell
analysis,
ensures
reliable
cell
isolation
quality
control
further
downstream
analyses.
Microfluidic
chips
small
lightweight
systems
that
efficient
high‐throughput
real‐time
analysis
on‐
or
off‐chip.
Therefore,
most
current
technologies
based
on
microfluidic
technology.
This
review
offers
comprehensive
guidance
to
researchers
across
different
fields
selection
appropriate
chip
analysis.
describes
design
principles,
separation
mechanisms,
characteristics,
cellular
effects
available
isolation.
Moreover,
this
highlights
implications
using
technology
subsequent
analyses,
including
Finally,
challenges
future
prospects
outlined
multiplex
Nanophotonics,
Год журнала:
2025,
Номер
14(8), С. 1045 - 1068
Опубликована: Янв. 17, 2025
Abstract
Enhancing
the
sensitivity
of
biomedical
spectroscopy
is
crucial
for
advancing
medical
research
and
diagnostics.
Metasurfaces
have
emerged
as
powerful
platforms
enhancing
various
spectral
detection
technologies.
This
capability
arises
from
their
unparalleled
ability
to
improve
interactions
between
light
matter
through
localization
enhancement
fields.
In
this
article,
we
review
representative
approaches
recent
advances
in
metasurface-enhanced
spectroscopy.
We
provide
a
comprehensive
discussion
technologies
enhanced
by
metasurfaces,
including
infrared
spectroscopy,
Raman
fluorescence
other
modalities.
demonstrate
advantages
metasurfaces
improving
sensitivity,
reducing
limits,
achieving
rapid
biomolecule
while
discussing
challenges
associated
with
design,
preparation,
stability
procedures.
Finally,
explore
future
development
trends
biological
emphasize
wide-ranging
applications.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(16), С. 6444 - 6449
Опубликована: Апрель 10, 2024
As
two
mainstream
ionic
detection
techniques,
current
rectification
(ICR)
suffers
from
large
fluctuations
in
trace
level
detection,
while
resistive-pulse
sensing
(RPS)
encounters
easy
clogs
high-concentration
detection.
By
rationally
matching
the
nanopore
size
with
DNA
tetrahedron
(TDN),
this
work
bridges
techniques
to
achieve
reliable
wide
linearity.
a
representative
analyte,
miRNA-10b
could
specifically
combine
and
release
TDN
interior
wall,
which
thus
induced
simultaneous
generation
of
distinct
ICR
RPS
signals.
The
signals
be
attributed
balance
between
effective
orifice
surface
charge
density
inner
were
by
complex
passing
through
nanopore.
Such
an
operation
contributed
range
1
fM-1
nM
good
feasibility
method
is
also
validated
single-cell
real
plasma
Nano Letters,
Год журнала:
2023,
Номер
23(17), С. 8249 - 8255
Опубликована: Авг. 29, 2023
Single-cell
analysis
of
the
DNA
repair
protein
is
important
but
remains
unachieved.
Exploration
nanopipettte
technologies
in
single-cell
electroanalysis
has
recently
seen
rapid
growth,
while
θ-nanopipette
represents
an
emerging
technological
frontier
with
its
potential
largely
veiled.
Here
a
first
applied
for
resistive-pulse
sensing
(RPS)
O6-alkylguanine
alkyltransferase
(hAGT).
The
removal
alkyl
mutations
by
hAGT
could
restore
damaged
aptamer
linking
structural
carrier,
allowing
selective
binding
to
thrombin
precisely
matched
size
produce
distinct
RPS
signals
when
passing
through
orifice.
Kinetic
was
studied.
Meanwhile,
device
shows
simultaneous
on-demand
infusion
inhibitors
inactivate
activity,
indicative
drug
screening
enhanced
chemotherapy.
This
work
provides
new
paradigm
θ-nanopipette-based
accompanied
evaluation.
Biosensors,
Год журнала:
2025,
Номер
15(4), С. 199 - 199
Опубликована: Март 21, 2025
Cancer
remains
one
of
the
leading
causes
death
worldwide.
Its
complex
pathogenesis
and
metastasis
pose
significant
challenges
for
early
diagnosis,
underscoring
urgent
need
innovative
non-invasive
tumor
screening
methods.
Exosomes,
small
extracellular
vesicles
that
reflect
physiological
pathological
states
their
parent
cells,
are
uniquely
suited
cancer
liquid
biopsy
due
to
molecular
cargo,
including
RNA,
DNA,
proteins.
However,
traditional
methods
exosome
isolation
detection
often
limited
by
inadequate
sensitivity,
specificity,
efficiency.
Nanopore
technology,
characterized
high
sensitivity
single-molecule
resolution,
offers
powerful
tools
analysis.
This
review
highlights
its
diverse
applications
in
screening,
such
as
magnetic
nanopores
high-throughput
sorting,
electrochemical
sensing
real-time
detection,
nanomaterial-based
assemblies
efficient
capture,
plasmon
resonance
ultrasensitive
These
advancements
have
enabled
precise
demonstrated
promising
potential
diagnosis
breast,
pancreatic,
prostate
cancers,
while
also
supporting
personalized
treatment
strategies.
Additionally,
this
summarizes
commercialized
products
exosome-based
diagnostics
examines
technical
translational
clinical
applications.
Finally,
it
discusses
future
prospects
nanopore
technology
advancing
toward
implementation.
The
continued
progress
not
only
accelerates
precision
medicine
but
represents
a
step
forward
next-generation
screening.
