ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(37), P. 49218 - 49226
Published: Sept. 6, 2024
As
an
important
biomarker,
tumor
cell-derived
exosomes
have
substantial
application
prospects
in
early
cancer
screening
and
diagnosis.
However,
the
unsatisfactory
sensitivity
complicated
sample
pretreatment
processes
of
conventional
detection
approaches
limited
their
use
clinical
Nanopore
sensors,
as
a
highly
sensitive,
label-free,
single-molecule
technology,
are
widely
utilized
molecule
bioparticle
detection.
Nevertheless,
exosome
capture
rate
through
nanopores
is
extremely
low
due
to
surface
charge
densities
effects
electrolyte
concentration
on
structural
stability,
thereby
reducing
throughput.
Here,
we
report
approach
improve
translocations
using
silicon
nitride
(SiN
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(28)
Published: May 20, 2024
Abstract
Single‐cell
multiomic
and
exosome
analyses
are
potent
tools
in
various
fields,
such
as
cancer
research,
immunology,
neuroscience,
microbiology,
drug
development.
They
facilitate
the
in‐depth
exploration
of
biological
systems,
providing
insights
into
disease
mechanisms
aiding
treatment.
isolation,
which
is
crucial
for
single‐cell
analysis,
ensures
reliable
cell
isolation
quality
control
further
downstream
analyses.
Microfluidic
chips
small
lightweight
systems
that
efficient
high‐throughput
real‐time
analysis
on‐
or
off‐chip.
Therefore,
most
current
technologies
based
on
microfluidic
technology.
This
review
offers
comprehensive
guidance
to
researchers
across
different
fields
selection
appropriate
chip
analysis.
describes
design
principles,
separation
mechanisms,
characteristics,
cellular
effects
available
isolation.
Moreover,
this
highlights
implications
using
technology
subsequent
analyses,
including
Finally,
challenges
future
prospects
outlined
multiplex
Nanophotonics,
Journal Year:
2025,
Volume and Issue:
14(8), P. 1045 - 1068
Published: Jan. 17, 2025
Abstract
Enhancing
the
sensitivity
of
biomedical
spectroscopy
is
crucial
for
advancing
medical
research
and
diagnostics.
Metasurfaces
have
emerged
as
powerful
platforms
enhancing
various
spectral
detection
technologies.
This
capability
arises
from
their
unparalleled
ability
to
improve
interactions
between
light
matter
through
localization
enhancement
fields.
In
this
article,
we
review
representative
approaches
recent
advances
in
metasurface-enhanced
spectroscopy.
We
provide
a
comprehensive
discussion
technologies
enhanced
by
metasurfaces,
including
infrared
spectroscopy,
Raman
fluorescence
other
modalities.
demonstrate
advantages
metasurfaces
improving
sensitivity,
reducing
limits,
achieving
rapid
biomolecule
while
discussing
challenges
associated
with
design,
preparation,
stability
procedures.
Finally,
explore
future
development
trends
biological
emphasize
wide-ranging
applications.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(16), P. 6444 - 6449
Published: April 10, 2024
As
two
mainstream
ionic
detection
techniques,
current
rectification
(ICR)
suffers
from
large
fluctuations
in
trace
level
detection,
while
resistive-pulse
sensing
(RPS)
encounters
easy
clogs
high-concentration
detection.
By
rationally
matching
the
nanopore
size
with
DNA
tetrahedron
(TDN),
this
work
bridges
techniques
to
achieve
reliable
wide
linearity.
a
representative
analyte,
miRNA-10b
could
specifically
combine
and
release
TDN
interior
wall,
which
thus
induced
simultaneous
generation
of
distinct
ICR
RPS
signals.
The
signals
be
attributed
balance
between
effective
orifice
surface
charge
density
inner
were
by
complex
passing
through
nanopore.
Such
an
operation
contributed
range
1
fM-1
nM
good
feasibility
method
is
also
validated
single-cell
real
plasma
Nano Letters,
Journal Year:
2023,
Volume and Issue:
23(17), P. 8249 - 8255
Published: Aug. 29, 2023
Single-cell
analysis
of
the
DNA
repair
protein
is
important
but
remains
unachieved.
Exploration
nanopipettte
technologies
in
single-cell
electroanalysis
has
recently
seen
rapid
growth,
while
θ-nanopipette
represents
an
emerging
technological
frontier
with
its
potential
largely
veiled.
Here
a
first
applied
for
resistive-pulse
sensing
(RPS)
O6-alkylguanine
alkyltransferase
(hAGT).
The
removal
alkyl
mutations
by
hAGT
could
restore
damaged
aptamer
linking
structural
carrier,
allowing
selective
binding
to
thrombin
precisely
matched
size
produce
distinct
RPS
signals
when
passing
through
orifice.
Kinetic
was
studied.
Meanwhile,
device
shows
simultaneous
on-demand
infusion
inhibitors
inactivate
activity,
indicative
drug
screening
enhanced
chemotherapy.
This
work
provides
new
paradigm
θ-nanopipette-based
accompanied
evaluation.
Aptamer-functionalized
nanopipettes
are
an
emerging
class
of
biosensors
for
the
label-free
detection
specific
molecules.
While
various
strategies
exist
immobilizing
single-stranded
DNA
aptamers
onto
inner
walls
glass
nanopipettes,
impact
fabrication
method
on
sensor
sensitivity,
signal
change,
reproducibility,
and
reliability
remains
unexplored.
In
this
study,
we
compared
three
methods
found
that
sensors
fabricated
using
gold
nanoparticles
(AuNPs)
synthesized
within
produced
most
reproducible
results
while
also
allowing
control
over
modification
process.
contrast,
two
other
aptamer
immobilization
methods,
which
relied
multistep
polymer
coatings
with
aminated
or
thiolated
coupling,
were
hindered
by
water
sensitivity
uneven
deposition,
resulting
in
inconsistent
responses.
Using
AuNP-coated
successfully
numerous
varying
sizes,
demonstrating
smaller
produce
greater
changes.
Sensors
constructed
diameters
ranging
from
22
to
30
nm
exhibited
large
changes
(>40%)
when
AuNP
synthesis
particles
near
tip
opening
without
causing
blockage.
However,
observed
significantly
lower
(using
same-sized
nanopipettes),
attributed
either
minimal
Au
present
at
conversely
blocked
probe.
These
highlight
critical
role
maximizing
enhancing
identifying
how
why
fail.
This
work
aims
facilitate
broader
adoption
aptamer-functionalized
analytical
sensing
applications.
Chinese Journal of Chromatography,
Journal Year:
2025,
Volume and Issue:
43(5), P. 399 - 412
Published: May 1, 2025
Exosomes
are
nanoscale
vesicles
secreted
by
cells
and
encapsulated
in
lipid
bilayers.
They
play
crucial
roles
cell
communication
involved
a
variety
of
physiological
pathological
processes,
including
immune
regulation,
angiogenesis,
tumor
initiation
metastasis.
carry
biomolecules
from
maternal
therefore
important
vehicles
for
discovering
disease
markers.
Traditional
detection
methods
only
provide
average
cell-population
information
given
sample
cannot
establish
clear
relationships
between
the
biological
functions
exosomes
subtype
owing
to
significant
heterogeneity
associated
with
different
subsets.
Therefore,
characterizing
at
single-cell
single-particle
levels
requires
exosome
specificities
be
further
explored
characteristics
various
subtypes
distinguished.
Commonly
used
characterization
technologies
include
flow
cytometry,
super-resolution
microscopy,
atomic
force
surface-enhanced
Raman
spectroscopy,
proximity
barcoding
assay
MS.
In
this
paper,
we
summarize
recent
advances
separation
based
on
microfluidics
future
applications
prospects
emerging
(such
as
Olink
proteomics,
click
chemistry,
molecular
imprinting)
studying
exosomes.
