Abstract
Tumor
metastasis
remains
a
leading
factor
in
the
failure
of
cancer
treatments
and
patient
mortality.
To
address
this,
silver‐induced
absorption
red‐shifted
core‐shell
nano‐particle
is
developed,
surface‐modified
with
triphenylphosphonium
bromide
(TPP)
hyaluronic
acid
(HA)
to
obtain
novel
nanodiagnosis‐treatment
agent
(Ag@CuS‐TPP@HA).
This
diagnosis‐treatment
can
dual‐targets
cells
mitochondria,
exhibits
maximal
light
at
1064
nm,
thereby
enhancing
nesr‐infrared
II
(NIR‐II)
photoacoustic
(PA)
signal
photothermal
effects
under
nm
laser
irradiation.
Additionally,
silver
Ag@CuS‐TPP@HA
catalyze
Fenton‐like
reactions
H
2
O
tumor
tissue,
yielding
reactive
oxygen
species
(ROS).
The
ROS
production,
coupled
enhanced
effects,
instigates
immunogenic
cell
death
(ICD),
substantial
release
tumor‐associated
antigens
(TAAs)
damage‐associated
molecular
patterns,
which
have
improved
immune
suppression
microenvironment
boosting
checkpoint
blockade
therapy,
thus
stimulating
systemic
antitumor
response.
Hence,
Ag@CuS‐TPP@HA,
as
diagnostic‐treatment
agent,
not
only
accomplishes
targeted
NIR‐II
PA
imaging
tissue
addresses
challenge
accurate
diagnosis
deep
vivo,
but
it
also
leverages
ROS/photothermal
therapy
enhance
blockade,
eliminating
primary
tumors
effectively
inhibiting
distant
growth.
Advanced Functional Materials,
Год журнала:
2023,
Номер
33(15)
Опубликована: Янв. 27, 2023
Abstract
Imbalance
of
macrophage
polarization
characterized
by
an
increase
in
the
percentage
pro‐inflammatory
M1
macrophages
and
a
decrease
anti‐inflammatory
M2
is
considered
critical
pathogenic
mechanism
bisphosphonate‐related
osteonecrosis
jaws
(BRONJ).
Because
high
levels
Toll‐like
receptor
4
(TLR4)
mediates
mitochondrial
dyshomeostasis
Zoledronic
Acid
(ZA)‐treated
macrophages,
tetrahedral
DNA
nanomaterial
(TDN)‐modified
with
TLR4‐siRNA
on
each
vertex
(TDN‐TLR4‐4siR)
excellent
biocompatibility
synthesized.
This
novel
TDN‐TLR4‐4siR
reverses
phenotype
imbalance
decreasing
RAW264.7
macrophages.
Mitochondrial
dynamics
analysis
shows
shift
from
short
rod‐like
ultrastructure
to
elongated
shapes
more
network
continuity
ZA‐primed
after
treatment
TDN‐TLR4‐4siR,
along
elevated
expression
Mfn1
Mfn2
.
further
reduces
intracellular
ROS
production
restored
membrane
potential.
Furthermore,
decreased
sequestra
formation
accelerated
healing
extraction
wound
are
observed
group,
resulting
incidence
rat
BRONJ
via
reprogramming
polarized
Consequently,
this
study
establishes
strategy
using
regulate
homeostasis
prevent
BRONJ.
Abstract
Cuproptosis,
an
emerging
form
of
programmed
cell
death,
has
received
tremendous
attention
in
cancer
therapy.
However,
the
efficacy
cuproptosis
remains
limited
by
poor
delivery
efficiency
copper
ion
carriers.
Herein,
complex
nanoparticles
(denoted
as
Cu(I)
NP)
are
developed
that
can
efficiently
deliver
into
cells
to
induce
cuproptosis.
NP
demonstrate
stimulus‐responsive
release
complexes,
which
results
mitochondrial
dysfunction
and
promotes
aggregation
lipoylated
dihydrolipoamide
S‐acetyltransferase
(DLAT),
leading
Notably,
not
only
cuproptosis,
but
also
elicit
robust
immune
responses
suppress
tumor
growth.
Overall,
this
study
provides
a
promising
strategy
for
cuproptosis‐based
Chemical Science,
Год журнала:
2023,
Номер
14(9), С. 2318 - 2324
Опубликована: Янв. 1, 2023
Herein,
giant
DNA
networks
were
assembled
from
two
kinds
of
functionalized
tetrahedral
nanostructures
(f-TDNs)
for
sensitive
detection
and
intracellular
imaging
apurinic/apyrimidinic
endonuclease
1
(APE1)
as
well
gene
therapy
in
tumor
cells.
Impressively,
the
reaction
rate
catalytic
hairpin
assembly
(CHA)
on
f-TDNs
was
much
faster
than
that
conventional
free
CHA
owing
to
high
local
concentration
hairpins,
spatial
confinement
effect
production
networks,
which
significantly
enhanced
fluorescence
signal
achieve
APE1
with
a
limit
3.34
×
10-8
U
μL-1.
More
importantly,
aptamer
Sgc8
could
enhance
targeting
activity
structure
cells,
allowing
it
endocytose
into
cells
without
any
transfection
reagents,
selective
living
Meanwhile,
siRNA
carried
by
f-TDN1
be
accurately
released
promote
cell
apoptosis
presence
endogenous
target
APE1,
realizing
effective
precise
therapy.
Benefiting
specificity
sensitivity,
developed
provide
an
excellent
nanoplatform
cancer
diagnosis
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(18)
Опубликована: Янв. 18, 2023
Abstract
DNA
nanotechnology
relies
on
the
structural
and
functional
information
encoded
in
nucleic
acids.
Specifically,
sequence‐guided
reconfiguration
of
acids
by
auxiliary
triggers
provides
a
means
to
develop
switches,
machines
stimuli‐responsive
materials.
The
present
Review
addresses
recent
advances
construction
applications
dynamic
reconfigurable
nanostructures,
networks
Dynamic
transformations
proceeding
within
engineered
origami
frames
or
between
tiles,
triggered
scaled
supramolecular
structures
are
addressed.
use
frameworks
assemble
chiroplasmonic
optical
devices
operate
switchable
chemical
processes
discussed.
Also,
operation
is
addressed,
design
“smart”
all‐DNA
materials
their
introduced.
Future
perspectives
nanostructures
presented.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(43), С. 23859 - 23873
Опубликована: Окт. 19, 2023
The
precise
control
of
the
artificially
induced
reactions
inside
living
cells
is
emerging
as
an
effective
strategy
for
regulation
cell
functions.
Nevertheless,
manipulation
assembly
exogenous
molecules
into
artificial
architectures
in
response
to
intracellular-specific
signals
remains
a
grand
challenge.
Herein,
we
achieve
self-assembly
deoxyribonucleic
acid
(DNA)
network
cancer
cells,
specifically
responding
telomerase,
and
realize
mitochondrial
interference
consequent
cellular
behaviors.
Two
functional
DNA
modules
were
designed:
mitochondria-targeting
branched
telomerase-responsive
linear
DNA.
Upon
uptake
by
telomerase
primer
responded
strand
displacement
reaction
was
triggered
reverse
transcription
thus
releasing
linker
from
afterward
hybridized
with
form
on
mitochondria.
interfered
function
mitochondria,
realizing
apoptosis
cells.
This
system
further
administered
nude
mouse
tumor
model,
showing
remarkable
suppression
growth.
We
envision
that
telomerase-mediated
intracellular
provides
promising
route
therapy.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(40), С. 21991 - 22008
Опубликована: Сен. 4, 2023
Senolytics,
which
eliminate
senescent
cells
from
tissues,
represent
an
emerging
therapeutic
strategy
for
various
age-related
diseases.
Most
senolytics
target
antiapoptotic
proteins,
are
overexpressed
in
cells,
limiting
specificity
and
inducing
severe
side
effects.
To
overcome
these
limitations,
we
constructed
self-assembling
targeting
with
intracellular
oligomerization
system.
Intracellular
aryl-dithiol-containing
peptide
occurred
only
inside
the
mitochondria
of
due
to
selective
localization
peptides
by
RGD-mediated
cellular
uptake
into
integrin
αvβ3-overexpressed
elevated
levels
reactive
oxygen
species,
can
be
used
as
a
chemical
fuel
disulfide
formation.
This
results
artificial
protein-like
nanoassembly
stable
α-helix
secondary
structure,
disrupt
mitochondrial
membrane
via
multivalent
interactions
because
has
weaker
integrity
than
that
normal
cells.
These
three
specificities
(integrin
αvβ3,
high
ROS,
weak
integrity)
work
combination;
therefore,
this
intramitochondrial
system
selectively
induce
apoptosis
without
effects
on
Significant
reductions
key
senescence
markers
amelioration
retinal
degeneration
were
observed
after
elimination
pigment
epithelium
senolytic
macular
mouse
model
aged
mice,
effect
was
accompanied
improved
visual
function.
provides
treatment
diseases
using
supramolecular
senolytics.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(36)
Опубликована: Июнь 14, 2024
Mitochondria,
one
of
the
most
important
organelles,
represent
a
crucial
subcellular
target
for
fundamental
research
and
biomedical
applications.
Despite
significant
advances
in
design
DNA
nanotechnologies
variety
bio-applications,
dearth
strategies
that
enable
mitochondria
targeting
molecular
imaging
therapy
remains
an
outstanding
challenge
this
field.
In
Minireview,
we
summarize
recent
progresses
on
emerging
application
nanotechnology
mitochondria-targeted
tumor
treatment.
We
first
highlight
engineering
mitochondria-localized
nanosensors
situ
detection
diverse
key
molecules
are
essential
to
maintain
mitochondrial
functions,
including
microRNA,
enzymes,
small
molecules,
metal
ions.
Then,
compile
developments
anti-tumor
therapy,
modularly
designed
nanodevices
delivery
therapeutic
agents,
programmed
assembly
interference.
will
place
emphasis
clarification
chemical
principles
how
nanobiotechnology
can
be
various
Finally,
remaining
challenges
future
directions
field
discussed,
hoping
inspire
further
development
advanced
toolkits
both
academic
clinical
regarding
mitochondria.
Analytical Chemistry,
Год журнала:
2025,
Номер
97(1), С. 382 - 391
Опубликована: Янв. 2, 2025
Evaluating
tumor
radiosensitivity
is
beneficial
for
the
prediction
of
treatment
efficacy,
customization
plans,
and
minimization
side
effects.
Tracking
mitochondrial
DNA
(mtDNA)
repair
process
helps
to
assess
as
mtDNA
determines
fate
cell
under
radiation-induced
damage.
However,
current
probes
developed
monitor
levels
enzymes
suffered
from
complex
synthesis,
uncontrollable
preparation,
limited
selectivity,
poor
organelle-targeting
ability.
Especially,
correlation
between
activity
inherent
tumors
has
not
yet
been
explored.
Here,
we
present
a
mitochondria-targeted
DNA-based
nanoprobe
(TPP-Apt-tFNA)
in
situ
monitoring
enzyme
evaluating
radiosensitivity.
TPP-Apt-tFNA
consists
tetrahedral
framework
precisely
modified
with
three
functional
modules
on
each
vertexes,
that
is,
cell-targeting
aptamer,
mitochondrion-targeting
moiety,
apurinic/apyrimidinic
endonuclease
1
(APE1)-responsive
molecule
beacon.
Once
selectively
internalized
by
cells,
targeted
mitochondrion
specifically
recognized
APE1
activate
fluorescence,
allowing
observation
activity.
The
showed
elevated
mitochondria
cells
oxidative
stress.
Moreover,
enabled
illumination
different
APE1-mediated
cycle
phases.
Furthermore,
using
vitro
vivo,
found
high
repair,
which
allowed
them
recover
lesions,
had
low
sensitivity
radiation
an
unsatisfactory
radiotherapy
outcome.
Our
work
provides
new
imaging
tool
exploring
roles
diverse
biological
processes
guiding
treatment.
