A Silver‐Induced Absorption Red‐Shifted Dual‐Targeted Nanodiagnosis‐Treatment Agent for NIR‐II Photoacoustic Imaging‐Guided Photothermal and ROS Simultaneously Enhanced Immune Checkpoint Blockade Antitumor Therapy DOI Creative Commons
Yulong Bai, Jing Hua, Jingjin Zhao

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 11(11)

Published: Dec. 31, 2023

Abstract Tumor metastasis remains a leading factor in the failure of cancer treatments and patient mortality. To address this, silver‐induced absorption red‐shifted core‐shell nano‐particle is developed, surface‐modified with triphenylphosphonium bromide (TPP) hyaluronic acid (HA) to obtain novel nanodiagnosis‐treatment agent (Ag@CuS‐TPP@HA). This diagnosis‐treatment can dual‐targets cells mitochondria, exhibits maximal light at 1064 nm, thereby enhancing nesr‐infrared II (NIR‐II) photoacoustic (PA) signal photothermal effects under nm laser irradiation. Additionally, silver Ag@CuS‐TPP@HA catalyze Fenton‐like reactions H 2 O tumor tissue, yielding reactive oxygen species (ROS). The ROS production, coupled enhanced effects, instigates immunogenic cell death (ICD), substantial release tumor‐associated antigens (TAAs) damage‐associated molecular patterns, which have improved immune suppression microenvironment boosting checkpoint blockade therapy, thus stimulating systemic antitumor response. Hence, Ag@CuS‐TPP@HA, as diagnostic‐treatment agent, not only accomplishes targeted NIR‐II PA imaging tissue addresses challenge accurate diagnosis deep vivo, but it also leverages ROS/photothermal therapy enhance blockade, eliminating primary tumors effectively inhibiting distant growth.

Language: Английский

Functionalized DNA Nanomaterials Targeting Toll‐Like Receptor 4 Prevent Bisphosphonate‐Related Osteonecrosis of the Jaw via Regulating Mitochondrial Homeostasis in Macrophages DOI
Tao Zhang,

Huangshui Ma,

Xiaolin Zhang

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(15)

Published: Jan. 27, 2023

Abstract Imbalance of macrophage polarization characterized by an increase in the percentage pro‐inflammatory M1 macrophages and a decrease anti‐inflammatory M2 is considered critical pathogenic mechanism bisphosphonate‐related osteonecrosis jaws (BRONJ). Because high levels Toll‐like receptor 4 (TLR4) mediates mitochondrial dyshomeostasis Zoledronic Acid (ZA)‐treated macrophages, tetrahedral DNA nanomaterial (TDN)‐modified with TLR4‐siRNA on each vertex (TDN‐TLR4‐4siR) excellent biocompatibility synthesized. This novel TDN‐TLR4‐4siR reverses phenotype imbalance decreasing RAW264.7 macrophages. Mitochondrial dynamics analysis shows shift from short rod‐like ultrastructure to elongated shapes more network continuity ZA‐primed after treatment TDN‐TLR4‐4siR, along elevated expression Mfn1 Mfn2 . further reduces intracellular ROS production restored membrane potential. Furthermore, decreased sequestra formation accelerated healing extraction wound are observed group, resulting incidence rat BRONJ via reprogramming polarized Consequently, this study establishes strategy using regulate homeostasis prevent BRONJ.

Language: Английский

Citations

58
Stimulus‐Responsive Copper Complex Nanoparticles Induce Cuproptosis for Augmented Cancer Immunotherapy DOI Creative Commons

Fuzhen Hu,

Jia Huang,

Tiejun Bing

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)

Published: Jan. 25, 2024

Abstract Cuproptosis, an emerging form of programmed cell death, has received tremendous attention in cancer therapy. However, the efficacy cuproptosis remains limited by poor delivery efficiency copper ion carriers. Herein, complex nanoparticles (denoted as Cu(I) NP) are developed that can efficiently deliver into cells to induce cuproptosis. NP demonstrate stimulus‐responsive release complexes, which results mitochondrial dysfunction and promotes aggregation lipoylated dihydrolipoamide S‐acetyltransferase (DLAT), leading Notably, not only cuproptosis, but also elicit robust immune responses suppress tumor growth. Overall, this study provides a promising strategy for cuproptosis‐based

Language: Английский

Citations

39
Target-mediated self-assembly of DNA networks for sensitive detection and intracellular imaging of APE1 in living cells DOI Creative Commons

Xue-Mei Zhou,

Ying Zhuo,

Ruo Yuan

et al.

Chemical Science, Journal Year: 2023, Volume and Issue: 14(9), P. 2318 - 2324

Published: Jan. 1, 2023

Herein, giant DNA networks were assembled from two kinds of functionalized tetrahedral nanostructures (f-TDNs) for sensitive detection and intracellular imaging apurinic/apyrimidinic endonuclease 1 (APE1) as well gene therapy in tumor cells. Impressively, the reaction rate catalytic hairpin assembly (CHA) on f-TDNs was much faster than that conventional free CHA owing to high local concentration hairpins, spatial confinement effect production networks, which significantly enhanced fluorescence signal achieve APE1 with a limit 3.34 × 10-8 U μL-1. More importantly, aptamer Sgc8 could enhance targeting activity structure cells, allowing it endocytose into cells without any transfection reagents, selective living Meanwhile, siRNA carried by f-TDN1 be accurately released promote cell apoptosis presence endogenous target APE1, realizing effective precise therapy. Benefiting specificity sensitivity, developed provide an excellent nanoplatform cancer diagnosis

Language: Английский

Citations

41
Dynamic Reconfigurable DNA Nanostructures, Networks and Materials DOI Creative Commons
Jianbang Wang, Zhenzhen Li, Itamar Willner

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(18)

Published: Jan. 18, 2023

Abstract DNA nanotechnology relies on the structural and functional information encoded in nucleic acids. Specifically, sequence‐guided reconfiguration of acids by auxiliary triggers provides a means to develop switches, machines stimuli‐responsive materials. The present Review addresses recent advances construction applications dynamic reconfigurable nanostructures, networks Dynamic transformations proceeding within engineered origami frames or between tiles, triggered scaled supramolecular structures are addressed. use frameworks assemble chiroplasmonic optical devices operate switchable chemical processes discussed. Also, operation is addressed, design “smart” all‐DNA materials their introduced. Future perspectives nanostructures presented.

Language: Английский

Citations

35
Telomerase-Mediated Self-Assembly of DNA Network in Cancer Cells Enabling Mitochondrial Interference DOI
Yanfei Guo, Siqi Li,

Zhaobin Tong

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(43), P. 23859 - 23873

Published: Oct. 19, 2023

The precise control of the artificially induced reactions inside living cells is emerging as an effective strategy for regulation cell functions. Nevertheless, manipulation assembly exogenous molecules into artificial architectures in response to intracellular-specific signals remains a grand challenge. Herein, we achieve self-assembly deoxyribonucleic acid (DNA) network cancer cells, specifically responding telomerase, and realize mitochondrial interference consequent cellular behaviors. Two functional DNA modules were designed: mitochondria-targeting branched telomerase-responsive linear DNA. Upon uptake by telomerase primer responded strand displacement reaction was triggered reverse transcription thus releasing linker from afterward hybridized with form on mitochondria. interfered function mitochondria, realizing apoptosis cells. This system further administered nude mouse tumor model, showing remarkable suppression growth. We envision that telomerase-mediated intracellular provides promising route therapy.

Language: Английский

Citations

28
Supramolecular Senolytics via Intracellular Oligomerization of Peptides in Response to Elevated Reactive Oxygen Species Levels in Aging Cells DOI
Sangpil Kim,

Jae‐Byoung Chae,

Dohyun Kim

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(40), P. 21991 - 22008

Published: Sept. 4, 2023

Senolytics, which eliminate senescent cells from tissues, represent an emerging therapeutic strategy for various age-related diseases. Most senolytics target antiapoptotic proteins, are overexpressed in cells, limiting specificity and inducing severe side effects. To overcome these limitations, we constructed self-assembling targeting with intracellular oligomerization system. Intracellular aryl-dithiol-containing peptide occurred only inside the mitochondria of due to selective localization peptides by RGD-mediated cellular uptake into integrin αvβ3-overexpressed elevated levels reactive oxygen species, can be used as a chemical fuel disulfide formation. This results artificial protein-like nanoassembly stable α-helix secondary structure, disrupt mitochondrial membrane via multivalent interactions because has weaker integrity than that normal cells. These three specificities (integrin αvβ3, high ROS, weak integrity) work combination; therefore, this intramitochondrial system selectively induce apoptosis without effects on Significant reductions key senescence markers amelioration retinal degeneration were observed after elimination pigment epithelium senolytic macular mouse model aged mice, effect was accompanied improved visual function. provides treatment diseases using supramolecular senolytics.

Language: Английский

Citations

24
Tetrahedral framework nucleic acids’ role in facilitating chronic diabetic wound repair via the endoplasmic reticulum-mitochondrial pathway DOI
Hao Wang,

Jinglei Gong,

Wen Chen

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 56, P. 102252 - 102252

Published: April 1, 2024

Language: Английский

Citations

12
DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics DOI
Fangzhi Yu, Xiang‐Fei Li,

Chuangui Sheng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(36)

Published: June 14, 2024

Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio-applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria-targeted tumor treatment. We first highlight engineering mitochondria-localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti-tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

Citations

12
Mitochondria-Targeted DNA-Based Nanoprobe for In Situ Monitoring of the Activity of the mtDNA Repair Enzyme and Evaluating Tumor Radiosensitivity DOI
Lanlan Chen, Jingjing Lai, Siqi Dong

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: 97(1), P. 382 - 391

Published: Jan. 2, 2025

Evaluating tumor radiosensitivity is beneficial for the prediction of treatment efficacy, customization plans, and minimization side effects. Tracking mitochondrial DNA (mtDNA) repair process helps to assess as mtDNA determines fate cell under radiation-induced damage. However, current probes developed monitor levels enzymes suffered from complex synthesis, uncontrollable preparation, limited selectivity, poor organelle-targeting ability. Especially, correlation between activity inherent tumors has not yet been explored. Here, we present a mitochondria-targeted DNA-based nanoprobe (TPP-Apt-tFNA) in situ monitoring enzyme evaluating radiosensitivity. TPP-Apt-tFNA consists tetrahedral framework precisely modified with three functional modules on each vertexes, that is, cell-targeting aptamer, mitochondrion-targeting moiety, apurinic/apyrimidinic endonuclease 1 (APE1)-responsive molecule beacon. Once selectively internalized by cells, targeted mitochondrion specifically recognized APE1 activate fluorescence, allowing observation activity. The showed elevated mitochondria cells oxidative stress. Moreover, enabled illumination different APE1-mediated cycle phases. Furthermore, using vitro vivo, found high repair, which allowed them recover lesions, had low sensitivity radiation an unsatisfactory radiotherapy outcome. Our work provides new imaging tool exploring roles diverse biological processes guiding treatment.

Language: Английский

Citations

1
Synthesis, Characterization, In Silico, and In Vitro studies of CuS-ZnO nanocomposite using mushroom extract DOI Creative Commons

S. Anbazhagi,

A. Murugesan,

M. Paramanantham

et al.

Hybrid Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100390 - 100390

Published: Jan. 1, 2025

Language: Английский

Citations

1