Macromolecular Bioscience,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Abstract
In
addition
to
the
20
standard
amino
acids
that
form
building
blocks
of
proteins,
nature
employs
alternative
create
specialized
“noncanonical
peptides.”
These
unique
peptides,
found
in
organisms
from
bacteria
humans,
often
exhibit
unconventional
structures
and
functionalities,
playing
critical
roles
modulating
cellular
processes,
particularly
as
antibiotics.
Their
potential
has
attracted
significant
interest
for
designing
novel
functional
materials
based
on
noncanonical
peptides.
This
review
highlights
recent
advances
generation
application
peptide
assemblies.
It
begins
with
a
definition
including
classic
examples
showcase
their
distinct
useful
biological
activities.
Then
applications
assemblies
developing
anticancer
therapeutics
are
discussed,
focusing
representative
studies
demonstrate
efficacy
versatility
targeting
tumor
cells.
Beyond
oncology,
it
is
explored
how
have
been
utilized
biomaterials,
regenerative
medicine,
molecular
imaging
catalysis.
Finally,
perspectives
offered
future
directions
this
rapidly
evolving
field,
emphasizing
exciting
opportunities
remaining
challenges
will
drive
continued
innovation
applying
peptide‐based
Abstract
The
term
“in
vivo
(“in
the
living”)
chemistry”
refers
to
chemical
reactions
that
take
place
in
a
complex
living
system
such
as
cells,
tissue,
body
liquids,
or
even
an
entire
organism.
In
contrast,
occur
generally
outside
organisms
artificial
environment
(e.g.,
test
tube)
are
referred
vitro.
Over
past
decades,
significant
contributions
have
been
made
this
rapidly
growing
field
of
chemistry,
but
it
is
still
not
fully
understood,
which
transformations
proceed
efficiently
without
formation
by‐products
how
product
environments
can
be
characterized.
Potential
applications
imagined
synthesize
drug
molecules
directly
within
cell
confer
new
cellular
functions
through
controlled
will
improve
understanding
systems
and
develop
therapeutic
strategies.
guiding
principles
contribution
twofold:
1)
Which
translated
from
laboratory
system?
2)
characterization
methods
suitable
for
studying
structure
environments?
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(8), С. 10532 - 10544
Опубликована: Фев. 17, 2024
Materials
made
of
assembled
biomolecules
such
as
amino
acids
have
drawn
much
attention
during
the
past
decades.
Nevertheless,
research
on
relationship
between
chemical
structure
building
block
molecules,
supramolecular
interactions,
and
self-assembled
structures
is
still
necessary.
Herein,
self-assembly
coassembly
fluorenylmethoxycarbonyl
(Fmoc)-protected
aromatic
(tyrosine,
tryptophan,
phenylalanine)
were
studied.
The
individual
Fmoc-Tyr-OH
Fmoc-Phe-OH
in
water
formed
nanofibers,
while
Fmoc-Trp-OH
into
nanoparticles.
Moreover,
when
or
was
coassembled
with
Fmoc-Trp-OH,
nanofibers
transformed
UV–vis
spectroscopy,
Fourier
transform
infrared
fluorescence
spectroscopy
used
to
investigate
interactions
leading
architectures.
π–π
stacking
hydrogen
bonding
main
driving
forces
forming
nanofibers.
Further,
a
mechanism
involving
two-step
process
proposed
based
nucleation
elongation/growth
explain
structural
transformation.
acted
fiber
inhibitor
alter
molecular
process,
locking
step
preventing
formation
This
transformation
useful
for
extending
application
acid
self-
materials
different
fields.
For
example,
could
be
applied
tissue
engineering,
they
exploited
drug
nanocarriers
form
Journal of Advanced Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 1, 2024
The
construction
of
gels
from
low
molecular
weight
gelators
(LMWG)
has
been
extensively
studied
in
the
fields
bio-nanotechnology
and
other
fields.
However,
understanding
gaps
still
prevent
prediction
LMWG
full
design
those
gel
systems.
Gels
with
multicomponent
become
even
more
complicated
because
multiple
interference
effects
coexist
composite
This
review
emphasizes
systems
view
on
(MLMWGs),
summarizes
recent
progress
desired
networks
MLMWGs,
including
self-sorting
co-assembly,
as
well
challenges
approaches
to
hope
that
opportunities
natural
products
peptides
can
speed
up
process
close
between
structures.
is
focused
three
key
concepts.
Firstly,
requires
a
perspective
MLMWGs.
Secondly,
several
protocols
be
applied
control
co-assembly
behaviors
system,
certain
complementary
structures,
chirality
inducing
dynamic
control.
Thirdly,
discussion
anchored
strategies
some
examples
are
provided
for
constructed
small
subtle
designed
short
peptides.
Chemical Science,
Год журнала:
2023,
Номер
14(7), С. 1724 - 1731
Опубликована: Янв. 1, 2023
A
protonation-activated
supramolecular
self-assembly
through
host–guest
interaction
was
successfully
constructed
in
lysosomes,
which
could
selectively
suppress
the
tumor
growth.
The
self-assembly
of
molecules
into
highly
ordered
architectures
is
a
ubiquitous
and
natural
process,
wherein
spontaneously
organize
large
structures
to
perform
diverse
functions.
Drawing
inspiration
from
the
formation
nanostructures,
cell-mediated
has
been
developed
create
functional
assemblies
both
inside
outside
living
cells.
These
techniques
have
employed
regulate
cellular
world
by
leveraging
dynamic
intracellular
extracellular
microenvironment.
This
review
highlights
recent
advances
future
trends
in
self-assembly,
ranging
their
cytocompatible
monomer
designs,
synthetic
strategies,
morphological
control
applications.
assembly
behaviors
are
also
discussed
based
on
dimensionality
self-assembled
morphologies
zero
three
dimensions.
Finally,
this
explores
its
promising
potential
for
biomedical
applications,
clarifying
relationship
between
initial
regulation
therapeutic
effects
subsequent
artificial
assemblies.
Through
rationally
designing
molecular
precisely
controlling
morphologies,
cell
mediated
would
provide
an
innovative
platform
executing
biological
Protein
carboxyl
methyltransferase
(PCMT)
restores
aspartate
isomers
in
proteins
and
plays
a
critical
role
cancer
prognosis.
However,
vivo
detection
of
PCMT
remains
challenging.
Here,
we
report
the
isomerization-regulated
situ
assembly
peptides
into
supramolecular
probes
within
living
cells
for
bladder
cancer.
The
peptide
consists
alternating
hydrophobic
hydrophilic
residues
contains
an
isoAsp
residue
as
kinked
site
to
prevent
facial
amphiphilicity
peptide.
Exposure
converts
Asp
peptide,
thereby
promoting
its
nanofibers.
Incorporation
7-nitro-2,1,3-benzoxadiazole
(NBD)
nanofibers
enables
based
on
hydrophobicity-dependent
fluorescence
NBD
units.
Both
cellular
animal
studies
confirm
capability
efficient
PCMT.
Our
finding
demonstrates
strategy
regulating
systems
thus
provides
new
tool
creation
biomedical
agents
future.
