Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 3, 2025
The
challenge
of
"false
positive"
signals
significantly
complicates
tumor
localization
and
surgical
resection,
which
are
pivotal
for
successful
surgeries.
Therefore,
the
development
a
method
preoperative
intraoperative
margin
determination
holds
considerable
promise
improving
outcomes.
In
this
study,
zero-crosstalk
ratiometric
tumor-targeting
near-infrared
(NIR)
fluorescent
probe
was
developed
precise
cancer
diagnosis
navigation
via
NIR
fluorescence
imaging.
This
integrates
moiety
that
selectively
homes
in
on
hepatocellular
carcinoma
cells
exhibits
highly
sensitive
response
to
γ-glutamyltranspeptidase
(GGT),
characterized
by
substantial
emission
shift
from
830
650
nm.
unique
property
enhances
its
effectiveness
early
imaging-guided
surgery
resection.
Furthermore,
successfully
monitors
GGT
activity
blood,
cells,
vivo,
endowing
potential
clinic.
Due
efficient
targeting
situ
both
subcutaneous
tumors
orthotopic
hepatic
tumors,
accurate
detection
capability
tumors.
Additionally,
leveraging
imaging,
can
serve
as
sprayable
tool
delineating
margins
with
precision,
thereby
furnishing
real-time
imaging
guidance
during
resection
surgery.
Chemical Society Reviews,
Год журнала:
2024,
Номер
53(8), С. 3976 - 4019
Опубликована: Янв. 1, 2024
This
review
focuses
on
the
design
strategy,
spectroscopic
characteristics,
and
functionalization
of
mitochondrion-targeted
BODIPY
dyes,
providing
an
overview
these
dyes
for
bioimaging
photodynamic
therapy.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(28)
Опубликована: Май 11, 2023
Heptamethine
cyanine
dyes
enable
deep
tissue
fluorescence
imaging
in
the
near
infrared
(NIR)
window.
Small
molecule
conjugates
of
benchmark
dye
ZW800-1
have
been
tested
humans.
However,
long-term
protocols
using
are
limited
by
their
instability,
primarily
because
chemically
labile
C4'-O-aryl
linker
is
susceptible
to
cleavage
biological
nucleophiles.
Here,
we
report
a
modular
synthetic
method
that
produces
novel
doubly
strapped
zwitterionic
heptamethine
dyes,
including
structural
analogue
ZW800-1,
with
greatly
enhanced
stability.
NIR-I
and
NIR-II
versions
these
can
be
conjugated
proteins,
monoclonal
antibodies,
without
causing
undesired
fluorophore
degradation
or
stacking
on
protein
surface.
The
fluorescent
antibody
show
excellent
tumor-targeting
specificity
xenograft
mouse
tumor
model.
stability
provided
molecular
design
will
new
classes
vivo
NIR
experiments
possible
translation
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(16)
Опубликована: Март 5, 2024
Fluorescence
imaging
in
the
second
near-infrared
window
(NIR-II)
is
burgeoning
because
of
its
higher
fidelity
monitoring
physiological
and
pathological
processes
than
clinical
visible/the
fluorescence
imaging.
Notably,
heavily
dependent
on
agents.
So
far,
indocyanine
green,
one
polymethine
dyes,
with
good
biocompatibility
renal
clearance
only
dye
approved
by
Food
Drug
Administration,
but
it
shows
relatively
low
NIR-II
brightness.
Importantly,
tremendous
efforts
are
devoted
to
synthesizing
dyes
for
preclinically
clinically.
They
have
shown
feasibility
customization
structure
properties
fulfill
various
needs
therapy.
Herein,
a
timely
update
special
focus
molecular
design
strategies
fluorescent,
photoacoustic,
multimodal
imaging,
offered.
Furthermore,
progress
sensing
biomarkers
even
reporting
drug
release
illustrated.
Moreover,
imaging-guided
therapies
summarized
regarding
chemo-,
photothermal,
photodynamic,
approaches.
In
addition,
artificial
intelligence
pointed
out
potential
expedite
development.
This
comprehensive
review
will
inspire
interest
among
wide
audience
offer
handbook
people
an
dyes.
Abstract
Intravital
fluorescence
imaging
in
the
second
near‐infrared
window
(NIR‐II,
900–1700
nm)
has
emerged
as
a
promising
method
for
non‐invasive
diagnostics
complex
biological
systems
due
to
its
advantages
of
less
background
interference,
high
tissue
penetration
depth,
contrast,
and
sensitivity.
However,
traditional
NIR‐II
imaging,
which
is
characterized
by
“always
on”
or
“turn
mode,
lacks
ability
quantitative
detection,
leading
low
reproducibility
reliability
during
bio‐detection.
In
ratiometric
can
realize
reliable
analysis
detection
vivo
providing
reference
signals
correction,
generating
new
opportunities
prospects
bioimaging
biosensing.
this
review,
current
design
strategies
sensing
mechanisms
probes
biosensing
applications
are
systematically
summarized.
Further,
challenges,
future
perspectives
designing
also
discussed.
It
hoped
that
review
provide
effective
guidance
promote
adoption
vivo.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(15), С. 6030 - 6036
Опубликована: Апрель 3, 2024
Cysteine
(Cys),
as
one
of
the
biological
thiols,
is
related
to
many
physiological
and
pathological
processes
in
humans
plants.
Therefore,
it
necessary
develop
a
sensitive
selective
method
for
detection
imaging
Cys
organisms.
In
this
work,
novel
near-infrared
(NIR)
fluorescent
probe,
Probe-Cys,
was
designed
by
connecting
furancarbonyl,
new
recognition
moiety,
with
Fluorophore–OH
via
decomposition
IR-806.
The
use
furan
moiety
anticipated
produce
more
effective
fluorescence
quenching
because
electron-donating
ability
O
atom.
Probe-Cys
has
outstanding
properties,
such
group,
an
emission
wavelength
infrared
region
at
710
nm,
linear
range
(0–100
μM),
low
limit
0.035
μM,
good
water
solubility,
excellent
sensitivity,
selectivity
without
interference
Hcy,
GSH,
HS–.
More
importantly,
could
achieve
endogenous
reacting
stimulant
1,4-dimercaptothreitol
(DTT)
inhibitor
N-ethylmaleimide
(NEM)
HepG2
cells
zebrafish.
Ultimately,
successfully
applied
obtain
images
Arabidopsis
thaliana,
revealing
that
content
meristematic
zone
higher
than
elongation
zone,
which
first
time
NIR
probe
used
A.
thaliana.
superior
properties
exhibit
its
great
potential
biosystems
explore
associated
Cys.
Journal of the American Chemical Society,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Liver
disease
poses
a
significant
challenge
to
global
health,
and
its
early
diagnosis
is
crucial
for
improving
treatment
outcomes
patient
prognosis.
Since
fluctuation
of
key
biomarkers
during
the
onset
progression
liver
diseases
can
directly
reflect
health
normal/abnormal
function,
biomarker-based
assays
are
vital
tools
detection
disease.
In
this
context,
small
molecule
fluorescent
probes
have
undeniably
emerged
as
indispensable
analysis,
with
an
ever-growing
number
molecule-based
being
developed
over
recent
years,
sole
aim
monitoring
relevant
This
perspective
will
focus
on
development
application
primarily
last
10
years
diagnosing
range
disease-related
processes.
It
outline
foundational
design
strategies
developing
promising
probes,
their
optical
response
biomarkers,
how
they
been
demonstrated
in
proof-of-concept
imaging
applications.
Current
challenges
new
developments
field
be
discussed,
providing
insights
highlighting
opportunities
field.
