Microorganisms,
Год журнала:
2020,
Номер
8(2), С. 308 - 308
Опубликована: Фев. 23, 2020
The
human
oral
cavity
is
home
to
an
abundant
and
diverse
microbial
community
(i.e.,
the
microbiome),
whose
composition
roles
in
health
disease
have
been
focus
of
intense
research
recent
years.
Thanks
developments
sequencing-based
approaches,
such
as
16S
ribosomal
RNA
metabarcoding,
whole
metagenome
shotgun
sequencing,
or
meta-transcriptomics,
we
now
can
efficiently
explore
diversity
microbes,
even
if
unculturable.
Recent
studies
charted
ecosystems
how
they
change
due
lifestyle
conditions.
As
progress,
there
increasing
evidence
important
role
microbiome
conditions,
which
are
not
limited
diseases
cavity.
This,
turn,
opens
new
avenues
for
microbiome-based
diagnostics
therapeutics
that
benefit
from
easy
accessibility
monitoring
manipulation.
Yet,
many
challenges
remain
ahead.
In
this
review,
survey
main
methodologies
currently
used
highlight
major
findings
enabled
by
these
approaches.
Finally,
discuss
future
prospects
field.
Gut,
Год журнала:
2018,
Номер
67(9), С. 1716 - 1725
Опубликована: Июнь 22, 2018
The
microbiome
has
received
increasing
attention
over
the
last
15
years.
Although
gut
microbes
have
been
explored
for
several
decades,
investigations
of
role
microorganisms
that
reside
in
human
attracted
much
beyond
classical
infectious
diseases.
For
example,
numerous
studies
reported
changes
microbiota
during
not
only
obesity,
diabetes,
and
liver
diseases
but
also
cancer
even
neurodegenerative
is
viewed
as
a
potential
source
novel
therapeutics.
Between
2013
2017,
number
publications
focusing
on
was,
remarkably,
12
900,
which
represents
four-fifths
total
40
years
investigated
this
topic.
This
review
discusses
recent
evidence
impact
metabolic
disorders
focus
selected
key
mechanisms.
aims
to
provide
critical
analysis
current
knowledge
field,
identify
putative
issues
or
problems
discuss
misinterpretations.
abundance
metagenomic
data
generated
comparing
diseased
healthy
subjects
can
lead
erroneous
claim
bacterium
causally
linked
with
protection
onset
disease.
In
fact,
environmental
factors
such
dietary
habits,
drug
treatments,
intestinal
motility
stool
frequency
consistency
are
all
influence
composition
should
be
considered.
cases
bacteria
Prevotella
copri
Akkermansia
muciniphila
will
discussed
examples.
European Journal of Immunology,
Год журнала:
2019,
Номер
49(10), С. 1457 - 1973
Опубликована: Окт. 1, 2019
These
guidelines
are
a
consensus
work
of
considerable
number
members
the
immunology
and
flow
cytometry
community.
They
provide
theory
key
practical
aspects
enabling
immunologists
to
avoid
common
errors
that
often
undermine
immunological
data.
Notably,
there
comprehensive
sections
all
major
immune
cell
types
with
helpful
Tables
detailing
phenotypes
in
murine
human
cells.
The
latest
techniques
applications
also
described,
featuring
examples
data
can
be
generated
and,
importantly,
how
analysed.
Furthermore,
tips,
tricks
pitfalls
avoid,
written
peer-reviewed
by
leading
experts
field,
making
this
an
essential
research
companion.
