Tackling exosome and nuclear receptor interaction: an emerging paradigm in the treatment of chronic diseases DOI Creative Commons

Babu Santha Aswani,

Mangala Hegde, Ravichandran Vishwa

и другие.

Military Medical Research, Год журнала: 2024, Номер 11(1)

Опубликована: Сен. 26, 2024

Abstract Nuclear receptors (NRs) function as crucial transcription factors in orchestrating essential functions within the realms of development, host defense, and homeostasis body. NRs have garnered increased attention due to their potential therapeutic targets, with drugs directed at demonstrating significant efficacy impeding chronic disease progression. Consequently, these pharmacological agents hold promise for treatment management various diseases. Accumulating evidence emphasizes regulatory role exosome-derived microRNAs (miRNAs) inflammation, progression, therapy resistance, primarily by modulating factors, particularly NRs. By exploiting inflammatory pathways such protein kinase B (Akt)/mammalian target rapamycin (mTOR), nuclear factor kappa-B (NF-κB), signal transducer activator 3 (STAT3), Wnt/β-catenin signaling, exosomes play a pivotal panorama physiology, pathology. The internalization modulates initiates diverse autocrine or paracrine signaling cascades, influencing processes recipient cells survival, proliferation, differentiation, metabolism, cellular defense mechanisms. This comprehensive review meticulously examines involvement exosome-mediated NR regulation pathogenesis ailments, including atherosclerosis, cancer, diabetes, liver diseases, respiratory conditions. Additionally, it elucidates molecular intricacies communication between via NRs, leading immunomodulation. Furthermore, outlines implications exosome-modulated prophylaxis delineates current limitations, provides insights into future perspectives. also presents existing on components emergence resistance.

Язык: Английский

Programming assembly of biomimetic exosomes: An emerging theranostic nanomedicine platform DOI Creative Commons
Xiao Xu, Limei Xu, Caining Wen

и другие.

Materials Today Bio, Год журнала: 2023, Номер 22, С. 100760 - 100760

Опубликована: Авг. 11, 2023

Exosomes have emerged as a promising cell-free therapeutic approach. However, challenges in large-scale production, quality control, and heterogeneity must be overcome before they can used clinically. Biomimetic exosomes containing key components of natural been assembled through extrusion, artificial synthesis, liposome fusion to address these limitations. These exosome-mimetics (EMs) possess similar morphology function but provide higher yields, faster size compared conventional exosomes. This article provides an overview the chemical biological properties various synthetic exosome systems, including nanovesicles (NVs), EMs, hybrid We highlight recent advances production applications nanobiotechnology discuss advantages, limitations, potential clinical programming assembly mimetics.

Язык: Английский

Процитировано

54
Graphene Sensor Arrays for Rapid and Accurate Detection of Pancreatic Cancer Exosomes in Patients’ Blood Plasma Samples DOI Creative Commons
Tianyi Yin, Lizhou Xu, Bruno M. G. Rosa

и другие.

ACS Nano, Год журнала: 2023, Номер 17(15), С. 14619 - 14631

Опубликована: Июль 20, 2023

Biosensors based on graphene field effect transistors (GFETs) have the potential to enable development of point-of-care diagnostic tools for early stage disease detection. However, issues with reproducibility and manufacturing yields sensors, but also Debye screening unwanted detection nonspecific species, prevented wider clinical use technology. Here, we demonstrate that our wafer-scalable GFETs array platform enables meaningful results. As a case study high relevance, an accurate robust portable GFET biosensor pancreatic ductal adenocarcinoma (PDAC) in patients' plasma through specific exosomes (GPC-1 expression) within 45 min. In order facilitate reproducible blood plasma, optimized analytical performance biosensors via application internal control channel test protocol. Based samples from 18 PDAC patients 8 healthy controls, arrays could accurately discriminate between two groups while being able detect cancer stages including 1 2. Furthermore, confirmed higher expression GPC-1 found concentration was average more than magnitude samples. We these characteristics cancerous are responsible increase number target surface graphene, leading improved signal response biosensors. This holds great promise tool rapid diagnosis cancer.

Язык: Английский

Процитировано

48
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

и другие.

Advanced Science, Год журнала: 2024, Номер 11(30)

Опубликована: Июнь 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Язык: Английский

Процитировано

41
Tumor-derived small extracellular vesicles in cancer invasion and metastasis: molecular mechanisms, and clinical significance DOI Creative Commons
Chi Zhang, Chaoying Qin, Saikat Dewanjee

и другие.

Molecular Cancer, Год журнала: 2024, Номер 23(1)

Опубликована: Янв. 19, 2024

Abstract The production and release of tumor-derived small extracellular vesicles (TDSEVs) from cancerous cells play a pivotal role in the propagation cancer, through genetic biological communication with healthy cells. TDSEVs are known to orchestrate invasion-metastasis cascade via diverse pathways. Regulation early metastasis processes, pre-metastatic niche formation, immune system regulation, angiogenesis initiation, matrix (ECM) remodeling, modulation, epithelial-mesenchymal transition (EMT) among pathways regulated by TDSEVs. MicroRNAs (miRs) carried within as double-edged sword can either promote or inhibit cancer progression. serve excellent markers for detection tumors, tumor metastases. From therapeutic point view, risk may be reduced limiting On other hand, represent promising approach vivo delivery cargo present review article discusses recent developments current views field research clinical applications.

Язык: Английский

Процитировано

39
Engineering Exosomes for Therapeutic Applications: Decoding Biogenesis, Content Modification, and Cargo Loading Strategies DOI Creative Commons
Mehrnoosh Bahadorani, Mahboobeh Nasiri,

Kristen Dellinger

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 7137 - 7164

Опубликована: Июль 1, 2024

Abstract: Exosomes emerge from endosomal invagination and range in size 30 to 200 nm. contain diverse proteins, lipids, nucleic acids, which can indicate the state of various physiological pathological processes. Studies have revealed remarkable clinical potential exosomes diagnosing prognosing multiple diseases, including cancer, cardiovascular disorders, neurodegenerative conditions. also be engineered deliver their cargo a specific target. However, further advancements are imperative optimize exosomes' diagnostic therapeutic capabilities for practical implementation settings. This review highlights applications, emphasizing engineering through simple incubation, biological, click chemistry techniques. Additionally, loading agents onto exosomes, utilizing passive active strategies, exploring hybrid artificial discussed. Keywords: exosome, drug delivery, diagnostics, therapeutics

Язык: Английский

Процитировано

18
Mesenchymal stem cells derived exosomes: a new era in cardiac regeneration DOI Creative Commons
Hossein Rayat Pisheh, Mahsa Sani

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 23, 2025

Язык: Английский

Процитировано

3
Research Advances of Engineered Exosomes as Drug Delivery Carrier DOI Creative Commons
Lianghui Huang,

Enguang Wu,

Jiawei Liao

и другие.

ACS Omega, Год журнала: 2023, Номер 8(46), С. 43374 - 43387

Опубликована: Ноя. 9, 2023

Exosomes are nanoscale vesicles secreted by living cells that have similar membrane composition to parental and carry a variety of proteins, lipids, nucleic acids. Therefore, exosomes certain biological activities play an important role in intercellular communication. On the basis its potential as carrier for drug delivery systems, been engineered compensate shortage natural through various engineering strategies improving efficiency, enhancing targeting tissues organs, extending circulating half-life exosomes. This review focuses on loading drugs different strategies, discussions exosome surface modification summarizes advantages disadvantages strategies. In addition, this provides overview recent applications number refractory relapsable diseases. has provide reference further research development

Язык: Английский

Процитировано

37
The Regulation of Exosome Generation and Function in Physiological and Pathological Processes DOI Open Access
Ying Wang,

Tong Xiao,

Chaoran Zhao

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 25(1), С. 255 - 255

Опубликована: Дек. 23, 2023

Exosomes, a type of extracellular vesicle with diameter approximately 100 nm that is secreted by all cells, regulate the phenotype and function recipient cells carrying molecules such as proteins, nucleic acids, lipids are important mediators intercellular communication. Exosomes involved in various physiological pathological processes immunomodulation, angiogenesis, tumorigenesis, metastasis, chemoresistance. Due to their excellent properties, exosomes have shown potential application clinical diagnosis treatment disease. The functions depend on biogenesis, uptake, composition. Thus, deeper understanding these regulatory mechanisms can help find new targets for disease therapy. Therefore, this review summarizes integrates recent advances entire biological process exosomes, starting from formation early-sorting endosomes (ESCs) plasma membrane invagination release fusion multivesicular bodies (MVBs) membrane, well interactions between cells. We also describe discuss exosome production tumor used cancer

Язык: Английский

Процитировано

28
Precise Identification and Profiling of Surface Proteins of Ultra Rare Tumor Specific Extracellular Vesicle with Dynamic Quantitative Plasmonic Imaging DOI

Chunhui Zhai,

Jiang Long, Jie He

и другие.

ACS Nano, Год журнала: 2023, Номер 17(17), С. 16656 - 16667

Опубликована: Авг. 28, 2023

Specific detection of tumor-derived EVs (tEVs) in plasma is complicated by nontumor and non-EV particles. To accurately identify tEVs profile their surface protein expression at single tEV resolution directly with clinical still an unmet need. Here, we present a Dynamic Immunoassay for Single Profiling (DISEP), kinetic assay based on plasmon resonance microscopy (SPRM) specific profiling. DISEP adopts pair low-affinity oligonucleotide probes to respectively label EV proteins functionalize SPRM biosensor interface. labeled the possess distinctive binding kinetics from nonspecific particles plasma, which permits accurate digital plasmonic counting EVs. We demonstrate recognizing target among 350-fold background high sensitivity (4677 per μL). Clinical samples were analyzed discriminate between pancreatic cancer patients (n = 40) healthy donors 45). With panel biomarker signatures (EpCAM, HER2, GPC1), only requires 10 μL primary sample each donor classify tumor area under curve 0.98. provides highly profiling strategy early diagnosis.

Язык: Английский

Процитировано

25
Clinical Impact of Multi-omics profiling of extracellular vesicles in cancer Liquid Biopsy DOI Creative Commons
Mrunal Kulkarni, Rishav Kar,

Srestha Ghosh

и другие.

The Journal of Liquid Biopsy, Год журнала: 2024, Номер 3, С. 100138 - 100138

Опубликована: Янв. 4, 2024

Extracellular vesicles (EVs) are the cell's secreted component. It is majorly classified into microvesicles, apoptotic bodies, and exosomes. Exosomes play a significant role in cancer development progression. Its molecular signature (DNA, RNA, Proteins, lipids) has more priority profiling current decade. In prevention, most challenging part early detection. EVs-based screening develops promising platform. Multi-Omics exosomes profiling-based liquid biopsy support detection efficient way. This approach provides detailed expression data (it may be inner cargos or surface express molecules). article highlited multi-omic exosome theranostics applications cancer, technical challenges, improvisation for future improvement.

Язык: Английский

Процитировано

16